• Natural Product Sciences
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• v.18 no.4
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• pp.227-232
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• 2012

Ten known compounds, 7-hydroxysauchinone (1), sauchinone (2), di-O-methyltetrahydrofuriguaiacin B (3), henricine (4), saucerneol K (5), meso-dihydroguaiaretic acid (6), (-)-guaiacin (7), (3R,4S)-4-(4-hydroxy-3-methoxyphenyl)-4-methoxy-3-methylbutan-2-one (8), (E)-7-(4-hydroxy-3-methoxyphenyl)-7-methylbut-8-en-9-one (9), and licarin A (10), were isolated from aerial part of Sarurus chinensis. The chemical structures of these compounds were determined on the basis of spectroscopic analyses including 2D NMR. Compounds 1 - 10 were evaluated for their cytotoxic activity against the HL-60, MCF-7, and A549 cancer cell lines in in vitro.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.521-523
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• 2001

White spot syndrome virus (WSSV) causes the most serious epizootic in cultured penaeid shrimp. The epizootic started in 1992, and spread through east and south east Asia and into other shrimp growing countries of the region. WSSV has also been reported from Penaeus chinensis cultured in Korean peninsula (Park et al., 1998; Kim et al., 1999). However, this paper deals with the WSSV infection in cultured and wild P. chinensis, purification and characterization of the pathogen, and the occurrence of other concomitant infection. (omitted)

• Proceedings of the Korean Society of Crop Science Conference
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• 1995.10a
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• pp.42-43
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• 1995

• Korean journal of applied entomology
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• v.33 no.4
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• pp.225-229
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• 1994

The methanol extracts from 30 species of oriental medicinal plants belonging to 24 families were tested for their lhicidal and antifeeding activit~es against diamondback moth (Plutello xylostella L) and tobacco cutworn (Spodoptera litura F.) by a leaf-dipping method at a concentration of 5, 000 ppm. The methanol extract from Copti chinensis only showed a potent larvicidal activity against P xylostello. Strong antifeeding activity against P. xylostello was observed from the extmds from Platycodon grandiflorurn, Codonopsis pilosula, Asomm sieboldii, Rhus chinensis and Uthospermum erythrorhizon And a potent antifeeding activity against S. liturn was obtained from Akebia quinata and Equlsetum hyemale extracts. A significant antifeeding activity against both species was obtained from R chinensis and C. chinensis extracts.

• Journal of Applied Biological Chemistry
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• v.43 no.3
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• pp.176-179
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• 2000

Nonpolar taxoides extracted from a large-scale cell culture of Taxus chinensis were isolated through the normal and reverse phase column chromatographies, and their compounds were identified via NMR spectroscopy. The complete separation method was systematically established and described. In dichloromethane, dissolved paclitaxel and other taxoids with hexane were precipitated during the purification of paclitaxel from the plant cell culture of T. chinensis through a large-scale process while the relatively nonpolar taxane derivatives remained dissolved in the hexane phase. 13-Deoxy baccatin III (I), baccatin VI (II), taxchinin I (III), $2{\alpha}$, $5{\alpha}$, $10{\beta}$, $14{\beta}$-tetraacetoxy-4(20), 11-taxadiene(IV), 1-deoxy baccatinVI(V), and taxayuntin C (VI) were isolated through column chromatography and identified via NMR spectroscopy. Compounds I and IV were found to the major components, aside from paclitaxel, in the plant cell culture of T. chinensis. The concentrations of I and IV were compared with the that concentration of the paclitaxel in each of plant cell culture. The possible applications of compounds I, II, IV, and V were discussed.

• Journal of Animal Science and Technology
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• v.63 no.1
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• pp.191-193
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• 2021

Lactococcus lactis is a fermentative lactic acid bacterium that is used extensively in food fermentations. The L. lactis strain K_LL005 was isolated from the grasshopper (Oxya chinensis sinuosa) gut in Korea. In this study, we reported the complete genome sequence of Lactococcus lactis K_LL005. The final complete genome assembly consist of one circular chromosome (2,375,093 bp) with an overall guanine + cytosine (G + C) content of 35.0%. Annotation results revealed 2,281 protein-coding sequences (CDSs), 19 rRNAs, and 68 tRNA genes. Lactococcus lactis K_LL005 has a gene encoding xylose metabolism such as xylR, xylA, and xylB (xylRAB).

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.2
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• pp.227-233
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• 2004

Chronic exposure to solar radiation, particularly ultraviolet (UV) light, causes a variety of adverse reactions on human skin, such as sunburn, photoaging and photocarcinogenesis. Free radicals and reactive oxygen species (ROS) caused by UV exposure or other environmental facts play critical roles in cellular damage. And, repeated-UV irradiation activated the expression of the matrix metalloproteinase (MMP) and induced skin irritation. Therefore, the development of effective and safe photoprotectants that can reduce and improve the skin damage has been required. The purpose of this study was to investigate the photo-protective effect of several chinese medical plants (Juniperus chinensis) on the UV -induced skin cell damages. We tested free radical and superoxide scavenging effect in vitro. Fluorometric assays of the proteolytic activities of MMP-1 (collagenase) were performed using fluorescent collagen substrates. UVA induced MMP-1 synthesis and activity were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in skin fibroblasts. We also examined anti-inflammatory effects by the determination test of proinflammatory cytokine, interleukin 6 in HaCaT keratinocytes. Expression of prostaglandin E$_2$ (PGE$_2$) after UVB irradiation was measured by competitive enzyme immunoassay(EIA) using PGE$_2$ monoclonal antibody. In the human skin we tested anti-irritation effect on the SLS-induced damage skin after appling the extract containing emulsion. We found that Juniperus chinensis extract had potent radical scavenging effect by 98% at 100$\mu\textrm{g}$/mL. The extract of Juniperus chinensis showed strong inhibitory effect on MMP-1 activities by 97% at 100 $\mu\textrm{g}$/mL and suppressed the UVA induced expression of MMP-1 by 79% at 25$\mu\textrm{g}$/mL. This extract also showed strong inhibition on MMP-2 activity in UVA irradiated fibroblast by zymography. In the test of proinflammatory cytokines of human keratinocytes Juniperus chinensis extract decreased expression of interleukin 6 about 30%. The amount of PGE$_2$ by HaCaT keratinocytes was significantly increased at the doses of above 10 mJ/$\textrm{cm}^2$ of UVB (p < 0.05). At the concentrations of 3.2-25$\mu\textrm{g}$/mL of this extract, the production of PGE$_2$ by HaCaT keratinocytes (24 h after 10mJ/$\textrm{cm}^2$ UVB irradiation) was significantly inhibited in culture supernatants (p < 0.05). In SLS-induced skin irritation model in vivo, we found to reduce skin erythema and improve barrier recovery after appling Juniperus chinensis extract containing emulsion when compared to irritated non-treated and placebo-treated skin. Our results suggest that Juniperus chinensis extract can be effectively used for the prevention of UV and SLS-induced adverse skin reactions and applied as anti-aging and anti-irritation cosmetics.

• Proceedings of the PSK Conference
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• 2000.04a
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• pp.185.1-185.1
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• 2000

• The Korean Journal of Malacology
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• v.17 no.1
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• pp.13-18
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• 2001

The relationship between shell length and ring radius in each ring group was expressed as a regression line. Therefore, there is a correspondence in each ring formation. Based on the monthly variation of the marginal index (Ml') of the shell, it is assumed that the ring of this species was formed once a year during the period of August to October, and the main period of the annual ring formation was August through September. The relationship between shell length (SL) and total weight (TW) was expressed by the equation TW = 2.2476 $\times$ 10$^{-5}$ SL$^{3.536}$ ($r^2$= 0.90). Shell length (SL) and shell height (SH; mm) were highly correlated with the equation SH = 0.7545 SL - 0.0145 ($r^2$= 0.93). The shell length (SL)-shell width (SW) relation was expressed by the equation SW = 0.5336 SL- 2.4253 ($r^2$= 0.87). Growth curves for shell length and total weight fitted to the von Bertalanffy's equation were expressed as follows: SL$_{t}$ =60.02[1 - e$^{-0.6458(t-0.3895)}$ ], Twt = 43.63[1 - e$^{0.6458(t-0.3895)}$ ]$^{3.536}$ .

• The Korean Journal of Pesticide Science
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• v.18 no.1
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• pp.8-13
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• 2014

This study evaluated in vitro cytotoxicity of 5 pesticides, including 2 herbicides, 2 germicides, and an insecticide, as an alternative to the fish acute toxicity test. The in vitro cytotoxicity was tested using a neutral red uptake (NRU) assay with epithelioma papulosum cyprini (EPC) cells that originated from the epidermal tissue of Cyprinus carpio (common carp). An in vivo fish acute toxicity test was conducted according to OECD Test Guideline No. 203 using Aphyocypris chinensis (Chinese bleak), Oryzias latipes (Japanese medaka), and C. carpio. The results showed that the sensitivity of the cell viability assay for the pesticides was similar to the fish acute test in ranking order despite having approximately 10 times less absolute sensitivity. The $r^2$ correlation values were calculated as 0.38 (p = 0.26), 0.76 (p = 0.05) and 0.90 (p = 0.01) for A. chinensis, O. latipes, and C. carpio, respectively. These results suggested that the potential of EPC cell viability assay as an alternative to the fish acute toxicity test due to their good correlation and NRU assay is expected to serve as a useful tool for predicting acute fish lethality for pesticides if further studies with a large set of pesticides are conducted.