• ALGAE
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• 제35권4호
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• pp.325-335
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• 2020

A new diatom species, Mayamaea vietnamica sp. nov., is described from Cát Tiên National Park in Vietnam. This species was discovered and described from soil samples. Algae from soil ecosystems in Vietnam are almost unknown. The new species is described on the basis of an integrated approach with molecular and morphological data, and comparison with similar species. In terms of molecular data, 18S rDNA (including V4 domain), and partial rbcL plastid genes show M. vietnamica sp. nov. is most closely related to M. terrestris N. Abarca and R. Jahn, and together they form a monophyletic group relative to other members of the genus. M. vietnamica sp. nov. differs from other species in the genus by the number of striae and areolae in 10 ㎛, number of areolae per stria, as well as shape and presence or absence of axial and central areas.

• 한국콘텐츠학회논문지
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• 제14권10호
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• pp.628-638
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• 2014

본 연구는 정신과 환자에게 한국판 회복평가척도(RAS-K)의 신뢰도와 타당도를 검사하기 위해 실시하였다. 회복평가척도의 원본을 가지고 번안, 역번안을 실시하여 한국판 회복평가척도를 만들었다. 이 연구는 만성적인 정신과적 문제를 가지고 있는 18세 이상의 142명의 대상자에게 실시하였다. 평가도구는 한국판 회복평가척도, 한국판 희망척도, 정신건강회복척도, 행동 및 증상 평가 척도를 사용하였다. 신뢰도 검사에서 내적일치도는 .92, 검사-재검사 신뢰도는 .79로 높은 내적일치도를 지니고 있음을 알 수 있었다. RAS-K와의 동시타당도는 한국판 희망평가척도(r=.675, p<0.01), 정신건강회복척도(r=.816, p<0.01)와는 유의미한 관계를 나타내었으나, 행동 및 증상 평가척도와는 관계는 낮았다. 요인분석 결과는 요인이 5개로 추출되었으며 전체 설명 비율이 62.476%였다. 이 연구는 RAS-K는 한국의 정신과적인 문제를 가진 사람들에게 회복을 평가하기에 적절한 도구라는 것을 보여준다.

• BMB Reports
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• 제47권12호
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• pp.685-690
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• 2014

The Ras/Raf/MEK/Erk signaling pathway is important for regulation of cell growth, proliferation, differentiation, survival, and apoptosis in response to a variety of extracellular stimuli. Lack of Erk MAPK activation is observed in several cancer cells despite active activation of Ras. However, little is known about the modulation of Erk1/2 activity by active Ras. Here, we show that overexpression of active H-Ras (H-RasG12R) in NIH3T3 fibroblasts impaired FGF2-induced Erk1/2 phosphorylation, as compared to wild-type cells. Northern blot analysis revealed that prolonged expression of active Ras increased MAP kinase phosphatase 3 (MKP3) mRNA expression, a negative regulator of Erk MAPK. Inhibition of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway abrogated active Ras-induced up-regulation of MKP3 expression, leading to the rescue of Erk1/2 phosphorylation. Our results demonstrated that the Ras/Raf/MEK/Erk signaling cascade is negatively regulated by the PI3K/Aktdependent transcriptional activation of the MKP3 gene.

• Molecules and Cells
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• 제43권10호
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• pp.889-897
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• 2020

K-RAS is frequently mutated in human lung adenocarcinomas (ADCs), and the p53 pathway plays a central role in cellular defense against oncogenic K-RAS mutation. However, in mouse lung cancer models, oncogenic K-Ras mutation alone can induce ADCs without p53 mutation, and loss of p53 does not have a significant impact on early K-Ras-induced lung tumorigenesis. These results raise the question of how K-Ras-activated cells evade oncogene surveillance mechanisms and develop into lung ADCs. RUNX3 plays a key role at the restriction (R)-point, which governs multiple tumor suppressor pathways including the p14^ARF-p53 pathway. In this study, we found that K-Ras activation in a very limited number of cells, alone or in combination with p53 inactivation, failed to induce any pathologic lesions for up to 1 year. By contrast, when Runx3 was inactivated and K-Ras was activated by the same targeting method, lung ADCs and other tumors were rapidly induced. In a urethane-induced mouse lung tumor model that recapitulates the features of K-RAS-driven human lung tumors, Runx3 was inactivated in both adenomas (ADs) and ADCs, whereas K-Ras was activated only in ADCs. Together, these results demonstrate that the R-point-associated oncogene surveillance mechanism is abrogated by Runx3 inactivation in AD cells and these cells cannot defend against K-Ras activation, resulting in the transition from AD to ADC. Therefore, K-Ras-activated lung epithelial cells do not evade oncogene surveillance mechanisms; instead, they are selected if they occur in AD cells in which Runx3 has been inactivated.

• Journal of Plant Biotechnology
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• 제45권3호
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• pp.273-278
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• 2018

We performed an analysis of the effect of postharvest treatment of rhizomes of Aconitum septentrionale Koelle with the biological preparation of Bacispecin based on a cytokinin producing strain of Paenibacillus ehimensis IB-739 on the content of alkaloid lappaconitine in rhizomes. The total alkaloid content was assayed based on exhaustive extraction method. The lappaconitine content was measured by HPLC. The efficiency of the preparation was dependant on either the concentration or intensity of the growth processes in rhizomes. Both parameters varied depending on the stage of development of plants and the amount of precipitation. In the years with normal precipitation, concentrations of Bacispecin ranging from 5 to 10 g/l were found to be most effective for increasing lappaconitine content in rhizomes of A. septentrionale. Whereas, under local drought conditions, the concentration of preparation should be less than 2 g/l.

• Carbon letters
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• 제3권3호
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• pp.142-145
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• 2002

Multiwall carbon nanotubes (MWNT) were produced using the arc-discharge graphite evaporation technique. Composite films were developed using MWNT dispersed in polystirol polymer. In the present work, various properties of the polymeric thin film containing carbon nanotubes were investigated by optical absorption, electrical resistivity and the same have been discussed.

• The Korean Journal of Physiology and Pharmacology
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• 제24권4호
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• pp.319-328
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• 2020

High fructose intake induces hyperglycemia and hypertension. However, the mechanism by which fructose induces metabolic syndrome is largely unknown. We hypothesized that high fructose intake induces activation of the renin-angiotensin system (RAS), resulting in hypertension and metabolic syndrome. We provided 11-week-old Sprague-Dawley rats with drinking water, with or without 20% fructose, for two weeks. We measured serum renin, angiotensin II (Ang II), and aldosterone (Aldo) using ELISA kits. The expression of RAS genes was determined by quantitative reverse transcription polymerase chain reaction. High fructose intake increased body weight and water retention, regardless of food intake or urine volume. After two weeks, fructose intake induced glucose intolerance and hypertension. High fructose intake increased serum renin, Ang II, triglyceride, and cholesterol levels, but not Aldo levels. High fructose intake increased the expression of angiotensinogen in the liver; angiotensin-converting enzyme in the lungs; and renin, angiotensin II type 1a receptor (AT1aR), and angiotensin II type 1b receptor (AT1bR) in the kidneys. However, expression of AT1aR and AT1bR in the adrenal glands did not increase in rats given fructose. Taken together, these results indicate that high fructose intake induces activation of RAS, resulting in hypertension and metabolic syndrome.

• 한국농공학회논문집
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• 제46권5호
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• pp.107-116
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• 2004

A GIS-based paddy inundation simulation system which is capable of simulating temporal and spatial inundation processes was established and applied in this paper. The system is composed of HEC-GeoHMS, and HEC-GeoRAS modules which interface the GIS and flood runoff models, and HEC-HMS, and HEC-RAS models which estimate the flood runoff. It was used to simulate storm runoff and inundation for a small rural watershed, the Baran HP#7, which is 10.69 $km^2$ in size. The simulated peak runoff, time to peak, and total direct runoff for eight storms were compared with the observed data. The results showed that the coefficient of determination ($R^2$) for the observed peak runoff was 0.99 and an error, RMSE, 11.862 $m^3$/s for calibration stages. In the model verification, $R^2$ was 0.99 and RMSE 1.296 $m^3$/s. Paddy inundation for each paddy growing stages in study watershed were estimated using verified inundation simulation system when probability rainfall was applied.

• 대한토목학회논문집
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• 제30권1C호
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• pp.45-51
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• 2010

지하철 터널 시공, 지하공간 시공, 대심도 굴착 등과 같이 도심지에서의 토목구조물 설계와 시공에 있어서 큰 어려움 중의 하나는 도심지 지반조사이다. 여러 가지 지장물, 전기 잡음, 교통 진동 등과 같은 자연적, 인위적 장애물로 인하여 어느 지반조사 기법이든지 그 결과의 신뢰성은 그리 높지 않은 상황이다. 본 연구에서는 이와 같이 현실적 요구가 높은 고품질 도심지 지반조사 기법의 개발을 목표로 하여 선행연구를 수행하였고, 일차적으로 HiRAS(Hybrid Integration of Resistivity and Surface Wave Velocities) 라고 하는 표면파-전기비저항 병합기법을 개발하였다. HiRAS 기법은 표면파 기법인 CapSASW 기법과 전기비저항 기법인 PDC-R 기법을 병합한 것으로서, 지반 매질의 강성 평가에 우수한 CapSASW 기법의 장점과, 전기잡음에 대한 내성과 지층변화 평가에 우수한 특성을 가진 PDC-R 기법의 장점을 동시에 활용하고자 한 것이다. 표면파 기법과 전기비저항 기법을 동시에 활용하는 역산해석의 측면에서는 표면파 기법의 탄성파 속도와 전기비저항 시험의 전기비저항 간의 부지고유관계를 이용하여 병합역산을 수행하는 전략을 채택하였다. 또한 HiRAS 기법 개발과정에서 부차적으로 지반매질의 포아송비 분포를 2차원으로 평가할 수 있는 성과도 도출하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제37권5호
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• pp.396-402
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• 2011

Introduction: Epidermal growth factor is a single-chain polypeptide consisting of 53 amino acids with potent mitogenic activity that stimulates the proliferation of a range of normal and neoplastic cells through an interaction with its specific receptor (epidermal growth factor receptor, EGFR). This interaction plays a key role in tumor progression including the induction of tumor cell proliferation. An increased EGFR copy number have been associated with a favorable response to EGFR tyrosine kinase inhibitors therapy. In contrast, K-ras mutations tend to predict a poor response to such therapy. The aim of this study was to determine the correlation between the clinicopathological factors and the up-regulation of EGFR expression and Kras mutations in oral squamous cell carcinoma. Materials and Methods: This study examined the immunohistochemical staining of EGFR, K-ras mutation detection with peptide nucleic acid (PNA)-based real-time polymerase chain reaction (PCR) clamping in 20 specimens from 20 patients with oral squamous cell carcinoma. Results: 1. In the immunohistochemical study of poorly differentiated and invasive oral squamous cell carcinoma, a high level of EGFR staining was observed. The correlation between immunohistochemical EGFR expression and histological differentiation, as well as the tumor size of the specimens was significant (Pearson correlation analysis, significance [r] >0.5, P<0.05). 2. In PNA-based real-time PCR clamping analysis, a K-ras mutation was not detected in all specimens. Conclusion: These findings suggest that the up-regulation of the EGFR may play a role in the progression and invasion of oral squamous cell carcinoma that is, independent of a K-ras mutation.