• Microbiology and Biotechnology Letters
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• v.28 no.4
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• pp.223-227
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• 2000

A novel two-step acetone treatment method was developed to enhance the enantioselectivity of Candida rugosa lipase (CRL) toward the hydrolysis of racemic naproxen methyl ester. The acetone-teated CRL was considerably more enantioselective than the crude CRL, yielding an enantiomeric excess of 98~100%. The crude and acetone-treated CRLs were subjected to anion exchange chromatography, and their chromatography profiles were compared. In consequence, both chromatography profiles were found to be almost identical, resulting in two separate lipase peaks (lipase A and B). The lipase B, which is known to be less enantioselective, was treated with acetone using a two-step treatment method. The enantioselectivity of acetone-treated lipase B was dramatically increased, yielding an enantiomeric excess of 99%.

• Journal of the Korean Society of Safety
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• v.13 no.1
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• pp.85-91
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• 1998

Sol-gel derived silica films were prepared by dip-coating onto polymethylmethacylate with Tetraethoxysilane(TEOS) as starting materials. Film properties such as viscosity and thickness were investigated as a function of dip speed, waterprecursor ratio, sol aging time. IR spectra of the gel films prepared from TEOS at various R are given. At small values of R the absorption peaks assignable to C-H vibration in $-OC_2H_5$ groups are observed around 3000 and 1500-1300 $cm^{-1}$. These bands indicate that the -$-OC_2H_5$ groups are retained in the gel at small values of R because of incomplete hydrolysis of TEOS. Film behaviour was interpreted in terms of the dependence of hydrolysis and condensation rates on the interplay between sol pH and waterprecursor ratio. Film thickness was found to increase by approximately a factor of two as waterprecursor ratio increased from two to six. Film thickness also increased with sol prepolymerization time. Surface quality was correlated with processing conditions.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.63.1-63.1
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• 2014

The galaxy disk warp is a common phenomenon, yet their properties and formation mechanism(s) are still unclear. Here, we introduce a new automatic measurement method for the warp properties of stellar disks in nearby edge-on galaxies, including warp's angle, shape, and asymmetry. We obtain isophotal maps of edge-on galaxies and express each of isophote contours in polar coordinates (${\Phi}$, R) centered on the galaxy centers. Two peaks in the ${\Phi}$-R diagram correspond to the outermost tips of each isophote. The locations of peaks, in turn, inform us of the misalignment between their inner and outer galactic planes, i.e., the warp. We apply this method to SDSS Stripe 82 co-added data and discuss its reliability and validity. Based on the measurement of warp properties, we also investigate their correlations with both intrinsic and environmental properties of warped galaxies.

• Journal of the Korean Solar Energy Society
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• v.25 no.4
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• pp.101-110
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• 2005

An experimental study of heat transfer of submerged water jet impinging normally on a flat plate is presented. Heat transfer measurements obtained with Reverse cone type nozzle(Rcone) were compared to those obtained with Cone type nozzle(Cone) and Square edged type nozzle(Vert) of the same diameter(D=8mm) for different jet velocities in the range of $3{\sim}7m/s(Re_D=30000{\sim}70000)$ and various nozzle-to target spacings($H/D=2{\sim}10$). The local Nusselt number profiles exhibited a sharp drop for $r/D{\leq}0.5$ and 2nd, 3rd peaks revealed at r/D=2, 3 respectively, followed by a slower decrease there after. The peaks were weakened with increasing the nozzle-to target spacing and decreasing the jet velocity. The stagnation Nusselt number of the Reverse cone type nozzle was larger than those of the other two nozzles for H/D=2. 10, but Cone type nozzle had the highest value for $H/D=4{\sim}8$. Also average Nusselt number of the Reverse cone type nozzle was higher than those of the other two nozzles at $H/D=2{\sim}10$, except for $V_o=7ms$ of H/D=6.

• Bulletin of the Korean Chemical Society
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• v.20 no.9
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• pp.1067-1072
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• 1999

Structures of unprotonated [(NH3)n+(n = 1-6)] and protonated [NH4+(NH3)n-1(n = 1-6)] ammonia cluster cations have been optimized with ab initio Hartree-Fock (HF) and second-order MФller-Plesset (MP2)/6-31+G ** levels and the harmonic vibrational frequencies have also been evaluated. In unprotonated cluster cations, NH3+ forms as a central core of the first ammonia solvation shell. In protonated cluster cations, NH4+ forms as a central core. In unprotonated dimer and trimer cations, there are two types of isomers (hydrogen-bonded and head-to-head interactions). In both cluster cations, the hydrogen-bonded isomers are more stable. In the hydrogen-bonded dimer cation, the proton transfer reaction takes place from (NH3-HN+H2) to (NH4+-NH2). But in the other unprotonated cluster cations, the proton transfer does not take place. In unprotonated pentamer and hexamer, a NH3+ core has both interactions in a complex. On the other hand, in unprotonated tetramer a core has only the hydrogen-bonded type combined with neutral ammonia molecules. With increasing cluster cation size, the bond lengths [R(NN)] between two nitrogen atoms and the distances [R(N ...H)] of the hydrogen-bond increase reg-ularly. In the calculated infra-red absorption bands for ammonia cluster cations, the characteristic peaks of the bridged NH vibration of the hydrogen-bonded clusters appear near 2500 cm-1 . With increasing size, the peaks shift from 2306 cm-1 to 2780 cm-1 .

• Mass Spectrometry Letters
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• v.10 no.2
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• pp.50-55
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• 2019

The fragmentation statistics of ion trap CID (Collision-Induced Dissociation) spectra using 87,661 tandem mass spectra of doubly charged tryptic peptides are analyzed here. In contrast to the usual method of using intensity information, the frequency of occurrence of fragment ions, with respect to the position of the cleavage site and the residues at these sites is studied in this paper. The analysis shows that the frequency of occurrence of fragment ion peaks is more towards the middle of the peptide than its ends. It was noted that amino acid with an aromatic and basic side chain at N- & C- terminal end of the peptide stimulates more peaks at the lower end of the spectrum. The residue pair effect was shown when the amide bond occurs between acidic and basic residues. The fragmentation at these sites (D/E-H/R/K) stimulates the generation of the y-ion peak. Also, the cleavage site H-H/R/K stimulates the generation of b-ions. K-P environment in the peptide sequence has more tendency to generate y-ions than b-ions. Statistical analysis helps in the visualization of the CID fragmentation pattern. Cleavage pattern along the length of the peptide and the residue pair effects, enhance the knowledge of fragmentation behavior, which is useful for the better interpretation of tandem mass spectra.

• Steel and Composite Structures
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• v.17 no.6
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• pp.891-906
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• 2014

This study aims to present a three dimensional finite element model to investigate the wave propagation in a concrete filled steel tubular column (CFSC) due to transient impact load. Both the concrete and steel are regarded as linear elastic material. The impact load is simulated by a semi sinusoidal impulse. Besides the CFSC models, a concrete column (CC) model is established for comparing under the same loading condition. The propagation characteristics of the transient waves in CFSC are analyzed in detail. The results show that at the intial stage of the wave propagation, the velocity waves in CFSC are almost the same as those in CC before they arrive at the steel tube. When the waves reach the column side, the velocity responses of CFSC are different from those of CC and the difference is more and more obvious as the waves travel down along the column shaft. The travel distance of the wave front in CFSC is farther than that in CC at the same time. For different wave speeds in steel and concrete material, the wave front in CFSC presents an arch shape, the apex of which locates at the center of the column. Differently, the wave front in CC presents a plane surface. Three dimensional effects on top of CFSC are obvious, therefore, the peak value and arrival time of incident wave crests have great difference at different locations in the radial direction. High-frequency waves on the waveforms are observed. The time difference between incident and reflected wave peaks decreases significantly with r/R when r/R < 0.6, however, it almost keeps constant when $r/R{\geq}0.6$. The time duration between incident and reflected waves calculated by 3D FEM is approximately equal to that calculated by 1D wave theory when r/R is about 2/3.

• Molecules and Cells
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• v.38 no.6
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• pp.496-505
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• 2015

A variant peak was detected in the analysis of RP-HPLC of rHu-EPO, which has about 7% relative content. Fractions of the main and the variant peaks were pooled separately and further analyzed to identify the molecular structure of the variant peak. Total mass analysis for each peak fraction using ESI-TOF MS shows differences in molecular mass. The fraction of the main peak tends to result in higher molecular masses than the fraction of the variant. The detected masses for the variant are about 600-1000 Da smaller than those for the main peak. Peptide mapping analysis for each peak fraction using Asp-N and Glu-C shows differences in O-glycopeptide profiles at Ser126. The O-glycopeptides were not detected in the fraction of the variant. It is concluded that the variant peak is non-O-glycosylated rHu-EPO and the main peak is fully O-glycosylated rHu-EPO at Ser126.

• International Journal of Automotive Technology
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• v.8 no.4
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• pp.493-504
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• 2007

This paper proposes a stereo vision-based forward obstacle detection and distance measurement method. In general, stereo vision-based obstacle detection methods in automotive applications can be classified into two categories: IPM (Inverse Perspective Mapping)-based and disparity histogram-based. The existing disparity histogram-based method was developed for stop-and-go applications. The proposed method extends the scope of the disparity histogram-based method to highway applications by 1) replacing the fixed rectangular ROI (Region Of Interest) with the traveling lane-based ROI, and 2) replacing the peak detection with a constant threshold with peak detection using the threshold-line and peakness evaluation. In order to increase the true positive rate while decreasing the false positive rate, multiple candidate peaks were generated and then verified by the edge feature correlation method. By testing the proposed method with images captured on the highway, it was shown that the proposed method was able to overcome problems in previous implementations while being applied successfully to highway collision warning/avoidance conditions, In addition, comparisons with laser radar showed that vision sensors with a wider FOV (Field Of View) provided faster responses to cutting-in vehicles. Finally, we integrated the proposed method into a longitudinal collision avoidance system. Experimental results showed that activated braking by risk assessment using the state of the ego-vehicle and measuring the distance to upcoming obstacles could successfully prevent collisions.

• ALGAE
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• v.19 no.2
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• pp.79-90
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• 2004

Nuclear DNA contents of spermatia from eight ceramiacean and four dasyacean algae (Ceramiales, Rhodophyta) and microspores from two land plants were estimated by fluorescence microscopic image processing and their nuclear SSU rDNA sequence data were analyzed. In frequency distribution patterns, the DAPI-stained nuclear volume (NV) of spermatia showed two peaks corresponding to 1C and 2C. Nuclear 2C DNA contents estimated from NV were 0.45-2.31 pg in ceramiacean and 0.40-0.57 pg in dasyacean algae and 8.42-9.51 pg in two land plants, Capsicum annuum and Nicotiana tabacum. By nuclear patterning of vegetative cells derived from an apical cell, 2C DNA contents of spermatia were 2.31 pg in an alga having uninucleate and non-polyploid nucleus (Aglaothamnion callophyllidicola), 0.45-1.94 pg in algae having uninucleate and polyploid nucleus (Antithamnion spp. and Pterothamnion yezoense), and 0.40-0.62 pg in algae having multinucleate and non-polyploid nuclei (Griffithsia japonica and dasyacean algae). Each mature spermatium and microspore (pollen grain) seemed to have a 2C nucleus, which may provide a genetic buffering system to protect the genetic content of a spermatium and microspore from potentially lethal mutations. Nuclear DNA content and SSU rDNA sequence of Antithamnion sparsum from Korea were reasonably different from those of Antithamnion densum from France. The data did not support the previous taxonomic studies that these two taxa could be conspecific.