• KSBB Journal
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• v.4 no.3
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• pp.229-234
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• 1989

NAD+ analogs, 8-( 6-aminohexyl) aminonicotinamide adenine dinucleotide and N6-[(6- aminohewl)-carbamoylmethyl]- NAD+, were imobilized on bovine caseins by the action of hansglutaminase. It appears that NAD+ analogs bind with $\alpha$S1-and $\beta$-caseins through formation of the r-glutamylamine bond between the amino groups attached to the hexyl chains in NAD+ analogs and the glutaminyl residues in caseins. The NAD+ analogs immobilized on the caseins were enzymatically reducible by alcohol dehydrogenase. $\beta$-Casein was more useful carrier than the $\alpha$S1-casein and 8-substituted NAD+ analog was more effective than N6-substituted one in immobilization. Michaelis constant of 8-substituted NAD+ analog immobilized on $\beta$-casein in alcohol dehydrogenase reaction was similar to that of free from of NAD+ and that of NAD+ analog. Immobilized NAD+ was much more stable at alkaline pH than free NAD+ and its analog while maximum velocity was reduced to 31% of the free NAD+ analog. The coenzyme casein conjugated was recovered almost completely in casein precipitated by calcium.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.05a
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• pp.523-526
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• 2010

FM Radio communication operating mode is half-duplex mode. FM radio network access control shall be used to detect the presence of active transmissions on a multiple-subscriber-access communications network and shall provide a means to preclude data transmissions from conflicting on the network. In this study, we implemented R-NAD(Random Network Access Delay) that is one of network access control method.

• Journal of the Korea Institute of Military Science and Technology
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• v.17 no.5
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• pp.561-568
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• 2014

The Republic of Korea Army is using R-NAD of MIL-STD-188-220 as a Media Access Control protocol. Under urgent situations, almost all stations transmit data frames and then the network will reach a saturation state. Several articles have been devoted to the study of R-NAD performance. However, most of them focus on comparing the performance of some NADs using network simulation tools. We propose an analytical model to compute the throughput of R-NAD under the assumption of a network traffic saturation. Analytical results were verified by Monte Carlo methods. We have shown that the performance of a success probability and an average idle time remains almost unchanged as the total number of stations increases. We have also shown that Type 1/2/4 operation mode outperforms Type 3 operation mode in throughput. The results showed that the system with a squelch detection achieved a better performance than the one without it. The longer DATA time had a higher throughput.

• Journal of Life Science
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• v.8 no.5
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• pp.486-491
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• 1998

Previously, we have cloned and characterized two ADP-ribosyltransferases (Yac-1 and Yac-2) from mouse Iym-phocyte. Yac-2 transferase contains significant NAD glycohydrolase activity as well as ADP-ribosyltransferase acti-vity. Yac-2 has an arginine at position 221 between two conserved glutamic acids. To investigate the significance of Arg-221 on enzyme activities, Arg-221 was mutagenized to Glu (R221E) and to Ala (R221A). Mutants R221E and R221A were active as wild type for ADP-ribosyltransferase and NAD glycohydrolase activity, suggesting that the arginine 221 in Yac-2 does not play a major role in enzyme activities.

• Journal of Adhesion and Interface
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• v.3 no.2
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• pp.45-52
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• 2002

기존의 점착제 합성방법에서 탈피하여 용액형 점착제(Solution)의 장점과 입자형 점착제(Emulsion)의 장점을 동시에 보유한 NAD (Non-Aqueous Dispersion Polymer)를 합성하였다. NAD는 메탄올 분위기에서 6개월 이상의 분산 안정성을 유지하였다. NAD는 용액에서 입자형 고분자의 형태를 이루고 있음을 도공 공정에서 확인하였다. 또한 기존의 아크릴 점착제의 장점과 고무계점착제의 장점을 결합한 고분자량의 점착제, GP (Graft Polymer)를 합성하였다. GP는 자체가 매우 높은 분자량을 보유하여 가교제의 첨가 없이도 우수한 물성을 나타내었다. GP는 일반적으로 고 분자량의 고분자에서 나타나는 Weissenberg effect 현상을 나타내었다. 이들의 점착제로 응용시 각각의 물성을 비교하여 나타내었다.

• Korean Chemical Engineering Research
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• v.54 no.1
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• pp.120-126
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• 2016

Eco-friendly acrylic resin/clay nanocomposites containing pristine montmorillonite (PM) or modified clays (30B and 25A) were prepared from acrylic and styrenic monomers using non-aqueous dispersion (NAD) polymerization. Effect of nanoclays on physical properties of polymerization product and resulting nanocomposites was investigated. In view of NAD particle stability, addition of nanoclay at the beginning of polymerization is proved to be good. Results of gel fraction, acid value and viscosity of the NAD product showed that nanocomposites containing clay 25A showed better physical properties than the ones with other clays. GPC results exhibit the increase in molecular weight and decrease in polydispersity index for the 25A nanocomposite. Increase in layer distance confirmed from XRD analysis showed good dispersion of 25A in the nanocomposite. Thermal and dynamic mechanical analysis showed that highest glass transition temperature and storage modulus for 25A nanocomposites. These results indicate that 25A nanoclay gives the best properties in the process of non-aqueous dispersion polymerization of acrylic resin/nanoclay nanocomposites.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.540-546
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• 2004

We deviced a new graphite-Mn(II) electrode and found that the modified electrode with Mn(II) can catalyze NADH oxidation and $NAD^+$ reduction coupled to electricity production and consumption as oxidizing agent and reducing power, respectively. In fuel cell with graphite-Mn(II) anode and graphite-Fe(III) cathode, the electricity of 1.5 coulomb (A x s) was produced from NADH which was electrochemically reduced by the graphite-Mn(II) electrode. When the initial concentrations of pyruvate and acetaldehyde were adjusted to 40 mM and 200 mM, respectively, about 25 mM lactate and 35 mM ethanol were produced from 40 mM pyruvate and 200 mM acetaldehyde, respectively, by catalysis of ADH and LDH in the electrochemical reactor with $NAD^+$ as cofactor and electricity as reducing power. By using this new electrode with catalytic function, the bioelectrocatalysts are engineered; namely, oxidoreductase (e.g., lactate dehydrogenase) and $NAD^+$ can function for biotransformation without electron mediator and second oxidoreductase for $NAD^+$/NADH recycling.

• Microbiology and Biotechnology Letters
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• v.7 no.3
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• pp.165-173
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• 1979

Recovery component for cell division in UV-irradiated E. coli B was detected with use of the cell extract of E. coli B/r which is a resistant mutant of E. coli B against UV-irradiation. The active substance was non-dialyzable and increased the activity by adding B-NAD remarkably. One more factor for increasing or promoting the restoration recognized was magnesium. Magnesium was effective to stabilze the substance in procedure of isolation. Two active substances were obtained from sucrose gradient centrifugation. One of them was recovred from the botton area and the other from top area just below below surface. the former was not stabilized by magnesium, while the latter stabilized the activity by it remarkably. The former which did not require magnesium was insensitive to protease and the latter which required magnesium was sensitive to it. Both were insensitive to RNase and DNase. Recovery ratio was doubled by using nitrogen gas than aeration in purification process. DNA-ligase less mutant was revealed same activity on it's recovery ratio with the parent strain of E. coli K-12. The active substance stimulating the filament cell may exist as a complex which is inactivated easily in the dissociated state ana requrie B-NAD or magnesium.

• Journal of Life Science
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• v.19 no.7
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• pp.852-858
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• 2009

The homeostasis of the pyridine nucleotide pool [NAD(P)H and NAD(P)$^+$] is maintained in Rhodobacter sphaeroides mutant strains defective in the cytochrome bci complex or the cytochrome c oxidases in terms of its concentration and redox state. Aerobic derepression of the puf operon, which is under the control of the PrrBA two-component system, in the CBB3 mutant strain of R. sphaeroides was shown to be not the result of changes in the redox state of the pyridine nucleotides and the ubiquinone/ubiquinol pool. Using the bc$_1$ complex knock-out mutant strain of R. sphaeroides, we clearly demonstrated that the inhibitory effect of cbb$_3$, oxidase on spectral complex formation is not caused indirectly by the redox change of the ubiquinone/ubiquinol pool.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.598-598
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• 2003