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Catalytic Oxidoreduction of Pyruvate/Lactate and Acetaldehyde/Ethanol Coupled to Electrochemical Oxidoreduction of $NAD^+$/NADH  

Shin, In-Ho (Department of Biological Engineering, Seokyeong University)
Jeon, Sung-Jin (Department of Biological Engineering, Seokyeong University)
Park, Hyung-Soo (Samsung Engineering R＆D Center)
Park, Doo-Hyun (Department of Biological Engineering, Seokyeong University)
Publication Information
Journal of Microbiology and Biotechnology / v.14, no.3, 2004 , pp. 540-546 More about this Journal
Abstract
We deviced a new graphite-Mn(II) electrode and found that the modified electrode with Mn(II) can catalyze NADH oxidation and $NAD^+$ reduction coupled to electricity production and consumption as oxidizing agent and reducing power, respectively. In fuel cell with graphite-Mn(II) anode and graphite-Fe(III) cathode, the electricity of 1.5 coulomb (A x s) was produced from NADH which was electrochemically reduced by the graphite-Mn(II) electrode. When the initial concentrations of pyruvate and acetaldehyde were adjusted to 40 mM and 200 mM, respectively, about 25 mM lactate and 35 mM ethanol were produced from 40 mM pyruvate and 200 mM acetaldehyde, respectively, by catalysis of ADH and LDH in the electrochemical reactor with $NAD^+$ as cofactor and electricity as reducing power. By using this new electrode with catalytic function, the bioelectrocatalysts are engineered; namely, oxidoreductase (e.g., lactate dehydrogenase) and $NAD^+$ can function for biotransformation without electron mediator and second oxidoreductase for $NAD^+$/NADH recycling.
Keywords
Electrochemical oxidoreduction; lactate dehydrogenase (LDH); alcohol dehydrogenase (ADH); graphite-Mn(II) electrode; NADH fuel cell;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 9  (Related Records In Web of Science)
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5 /
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16 /
[ Messing,R.A.(ed.) ] / Immobilized Enzymes for Industrial Reactor
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