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Expression of Arabidopsis thaliana SIK (Stress Inducible Kinase) Gene in a Potato Cultivar (Solanum tuberosum L. 'Taedong Valley')

  • Yoon Jung-Ha;Fang Yi-Lan;Park Eung-Jun;Kim Hye-Jin;Na Yun-Jeong;Lee Dong-Hee;Yang Deok-Chun;Lim Hak-Tae
    • Plant Resources
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    • v.8 no.3
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    • pp.202-208
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    • 2005
  • Osmotic stress is one of major limiting factors in crop production. In particular, seasonal drought often causes the secondary disease in the field, resulting in severe reduction in both quality and productivity. Recent efforts have revealed that many genes encoding protein kinases play important roles in osmotic stress signal transduction pathways. Previously, the AtSIK (Arabidopsis thaliana Stress Inducible Kinase) mutants have shown to enhance tolerance to abiotic stresses, accompanying with higher expression of abiotic stress-related genes than did the wild-type plants. In this study, we have transformed potato (cv. Taedong Valley) with the AtSIK expression cassette. Both PCR and RT-PCR using AtSIK-specific primers showed stable integration and expression of the AtSIK gene in individual transgenic lines, respectively. Foliar application of herbicide ($Basta^{(R)}$) at commercial application rate (0.3% (v/v)) revealed another evidence of stable gene introduction of T-DNA which includes the bar gene for herbicide resistance. Overexpression of the AtSIK gene under dual CaMV35S promoter increased sensitivity to salt stress (300 mM NaCl), which was demonstrated by the reduction rate of chlorophyll contents in leaves of transgenic potato lines. These results suggest that possible increase of osmotic tolerance in potato plants may be achieved by antisense expression of AtSIK gene.

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Equimolar Carbon Dioxide Absorption by Ether Functionalized Imidazolium Ionic Liquids

  • Sharma, Pankaj;Park, Sang-Do;Park, Ki-Tae;Jeong, Soon-Kwan;Nam, Sung-Chan;Baek, Il-Hyun
    • Bulletin of the Korean Chemical Society
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    • v.33 no.7
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    • pp.2325-2332
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    • 2012
  • A series $[C_3Omim]$[X] of imidazolium cation-based ILs, with ether functional group on the alkyl side-chain have been synthesized and structure of the materials were confirmed by various techniques like $^1H$, $^{13}C$ NMR spectroscopy, MS-ESI, FTIR spectroscopy and EA. More specifically, the influence of changing the anion with same cation is carried out. The absorption capacity of $CO_2$ for ILs were evaluated at 30 and $50^{\circ}C$ at ambient pressure (0-1.6 bar). Ether functionalized ILs shows significantly high absorption capacity for $CO_2$. In general, the $CO_2$ absorption capacity of ILs increased with a rise in pressure and decreased when temperature was raised. The obtained results showed that absorption capacity reached about 0.9 mol $CO_2$ per mol of IL at $30^{\circ}C$. The most probable mechanism of interaction of $CO_2$ with ILs were investigated using FTIR spectroscopy, $^{13}C$ NMR spectroscopy and result shows that the absorption of $CO_2$ in ether functionalized ILs is a chemical process. The $CO_2$ absorption results and detailed study indicates the predominance of 1:1 mechanism, where the $CO_2$ reacts with one IL to form a carbamic acid. The $CO_2$ absorption capacity of ILs for different anions follows the trend: $BF_4$ < DCA < $PF_6$ < TfO < $Tf_2N$. Moreover, the as-synthesized ILs is selective, thermally stable, long life operational and can be recycled at a temperature of $70^{\circ}C$ or under vacuum and can be used repeatedly.

Suggested and Preferred Amount of Clothing in a Winter Indoor Condition

  • Shim, Huen-Sup;Jeong, Woon-Seon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.35 no.12
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    • pp.1418-1424
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    • 2011
  • This study proposes the suggested amount of clothing (SAC) and examines the preferred amount of clothing (PAC) for thermal comfort in mild cold conditions. Six male and nine female college students were systematically exposed to mild cold conditions by reducing the amount of clothing (Step I, 1.2clo ${\rightarrow}$ Step II, 1.0clo ${\rightarrow}$ Step III, 0.8clo ${\rightarrow}$ Step IV, 0.7clo). The subjects were then asked to adjust the amount of clothing to attain overall thermal comfort until they maintained thermal comfort for 10 minutes without changing the amount of clothing (Step V). The experiment was carried out in a climatic chamber at $19.5^{\circ}C$, 50%R.H. Body composition was measured and individual cold climate adaptability was surveyed before starting the experiment. Rectal temperature ($T^{re}$), skin temperature ($T_{sk}$), and oxygen consumption ($\dot{V}O_2$) were measured and the overall thermal sensation was voted in each step. PAC was obtained from the garments weight selected by each subject in Step V. SAC was proposed based on the change of oxygen consumption (${\Delta}\dot{V}O_2$). As a result, males showed higher $\bar{T}_{sk}$ and greater $O_2$ than females (p<.01). SAC obtained from $\dot{V}O_2$ were 652.0 (SE 3.9) g/$m^2$ for males and 766.0 (SE 2.5) g/$m^2$ for females and it was significantly different between groups (p<.01). PAC of males and females were 1.6 and 1.5 times heavier than SAC. In conclusion, females were more sensitive to the cold stress and recommended larger amount of clothing than males.

Development of a Rapeseed Reaping Equipment Attachable to a Conventional Combine (Ill) - Analysis of Principal Factor for Loss Reduction of Rapeseed Mechanical Harvesting - (보통형 콤바인 부착용 유채 예취장치 개발 (III) - 유채 기계 수확 손실 절감을 위한 요인 구명 -)

  • Lee, C.K.;Choi, Y.;Jun, H.J.;Lee, S.K.;Moon, S.D.;Kim, S.S.
    • Journal of Biosystems Engineering
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    • v.34 no.2
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    • pp.114-119
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    • 2009
  • Field test was conducted to investigate primary factors reducing rapeseed harvesting using a reciprocating cutter-bar of combine. The results showed that the correlation between crop moisture content and yield loss had a U-type, which indicated that the yield reduction increased at too high and too low crop moisture contents. The proper ranges of crop moisture contents were 27${\sim}$35%, 21${\sim}$56%, and 62${\sim}$73% in case of grain, pod and stem, respectively. Crop moisture content was negatively correlated with header loss, but positively correlated with threshing loss. In contrary, stem moisture content showed positive correlations with total loss, threshing loss and separation loss. Working speed was positively correlated with header loss. Total flow rate, pod flow rate and stem flow rate were highly correlated with threshing loss and separation loss. However, grain flow rate did not show any correlation with total loss. According to the principal component analysis, two principal components were derived as components with eigenvalues greater than 1.0. The contribution rates of the first and the second components were 52.7% and 38.9%, which accounted for 91.6% of total variance. As a contributive factor influencing total loss of rapeseed mechanical harvesting, a crop moisture content factor was greater than a crop flow rate factor. The stepwise multiple regression analysis for total loss was conducted using crop moisture content factor, crop flow rate factor and coefficient. However, the model did not show any correlation among independent and dependent factors ($R^2$=0.060).

An Study on the Cylinder Wall Temperature and Performance of Gasoline Engine according to Engine Speed (가솔린기관의 회전수 변화에 따른 실린더 벽면온도 변화 및 기관성능에 관한 연구)

  • Kwon, K.R.;Oho, Y.O.;Kang, N.H.
    • Journal of Power System Engineering
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    • v.6 no.1
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    • pp.20-26
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    • 2002
  • The purpose of this study is preventing the stick, scuffing, scratch between piston and cylinder in advance, and obtaining data for duration test in actual engine operation. The temperature gradient in cylinder bore according to coolant temperature were measured using $1.5{\ell}$ class diesel engine. 20 thermocouples were installed 2mm deep inside from cylinder wall near top ring of piston in cylinder block, at which points major thermal loads exist. It is suggested as proper measurement points for engine design by industrial engineers. Under full load and $70^{\circ}$, $80^{\circ}C$ and $90^{\circ}C$ coolant temperature conditions, the temperature in cylinder block and engine oil increased gradually according to the increase of coolant temperature, the siamese side temperature of top dead center is $142^{\circ}C$ in peripheral distribution, that is about $20^{\circ}C$ higher than that at thrust, anti-thrust, and rear side temperature, respectively. The maximum pressure of combustion gas in $70^{\circ}C$ coolant temperature is about 2 bar lower than those of $80^{\circ}C$ and $90^{\circ}C$ coolant temperature. The engine torque in $80^{\circ}C$, $90^{\circ}C$ coolant temperature condition is about 4.9Nm higher than that of $70^{\circ}C$ coolant temperature.

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Cathodic Properties of $LiCoO_2$ Synthesized by a Sol-Gel Method for Lithium Ion Battery

  • 조봉준;정의덕;심윤보
    • Bulletin of the Korean Chemical Society
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    • v.19 no.1
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    • pp.39-44
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    • 1998
  • $LiCoO_2$ powder was synthesized in an aqueous solution by a sol-gel method and used as a cathode active material for a lithium ion rechargeable battery. The layered $LiCoO_2$ powders were prepared by igniting in air for 12 hrs at 600 ℃ $(600-LiCoO_2)$ and 850 ℃ $(850-LiCoO_2)$. The structure of the $LiCoO_2$ powder was assigned to the space group R bar 3 m (lattice parameters a=2.814 Å and c=14.04Å). The SEM pictures of $600-LiCoO_2$ revealed homogeneous and fine particles of about 1 μm in diameter. Cyclic voltammograms (CVs) of $600-LiCoO_2$ electrode displayed a set of redox peaks at 3.80/4.05 V due to the intercalation/deintercalation of the lithium ions into/out of the $LiCoO_2$ structure. CVs for the $850-LiCoO_2$ electrode had a major set of redox peaks at 3.88/4.13 V, and two small set of redox peaks at 4.18/4.42 V and 4.05/4.25 V due to phase transitions. The initial charge-discharge capacity was 156-132 mAh/g for the $600-LiCoO_2$ electrode and 158-131 mAh/g for the $850-LiCoO_2$ electrode at the current density of 0.2 mA/cm2. The cycleability of the cell consisting of the $600-LiCoO_2$ electrode was better than that of the $850-LiCoO_2$. The diffusion coefficient of the $Li^+$ ion in the $600-LiCoO_2$ electrode was calculated as $4.6{\times}10^{-8}\; cm^2/sec$.

Effect of chemical input during wet air oxidation pretreatment of rice straw in reducing biomass recalcitrance and enhancing cellulose accessibility

  • Morone, Amruta;Chakrabarti, Tapan;Pandey, R.A.
    • Korean Journal of Chemical Engineering
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    • v.35 no.12
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    • pp.2403-2412
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    • 2018
  • The present study was aimed at evaluating the effect of variable sodium carbonate ($Na_2CO_3$) loading during wet air oxidation (WAO) pretreatment of rice straw in reducing biomass recalcitrance. The research study was intended to increase the cellulose recovery, hemicellulose solubilization, lignin removal in the solid fraction and limiting the generation of inhibitors in the liquid fraction while reducing the chemical input. The operating condition of $169^{\circ}C$, 4 bar, 18 min and 6.5 g/L $Na_2CO_3$ loading resulted in maximum cellulose recovery of 82.07% and hemicellulose solubilization and lignin removal of 85.43% and 65.42%, respectively, with a total phenolic content of 0.36 g/L in the liquid fraction. The crystallinity index increased from 47.69 to 51.25 along with enzymatic digestibility with an increase in $Na_2CO_3$ loading from 0 to 6.5 g/L as a result of removal of barriers for saccharification via effective cleavage of ether and ester bonds cross-linking the carbohydrates and lignin as indicated by FT-IR spectroscopy. A further increase in the $Na_2CO_3$ loading to 9.5 g/L did not significantly increase the sugar release. Thus, it was concluded that 6.5 g/L $Na_2CO_3$ during WAO is sufficient to increase the delignification and deacetylation, leading to significant changes in apparent cellulose crystallinity inter alia improvement in cellulose accessibility and digestibility of rice straw.

Hydrodynamics and Solid Circulation Characteristics of Oxygen Carrier for 0.5 MWth Chemical Looping Combustion System (0.5 MWth 케미컬루핑 연소시스템 적용을 위한 산소전달입자의 수력학 특성 및 고체순환 특성)

  • RYU, HO-JUNG;KIM, JUNGHWAN;HWANG, BYUNG WOOK;NAM, HYUNGSEOK;LEE, DOYEON;JO, SUNG-HO;BAEK, JEOM-IN
    • Journal of Hydrogen and New Energy
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    • v.29 no.6
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    • pp.635-641
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    • 2018
  • To select the operating condition of 0.5 MWth chemical looping combustion system, minimum fluidization velocity, transition velocity to fast fluidization and solid circulation rate were measured using mass produced new oxygen carrier (N016-R4) which produced by spray drying method for 0.5 MWth chemical looping combustion system. A minimum fluidization velocity decreased as the pressure increased. The measured transition velocity to fast fluidization was 2.0 m/s at ambient temperature and pressure. The measured solid circulation rate increased as the solid control valve opening increased. We could control the solid circulation rate from 26 to $93kg/m^2s$. Based on the measured minimum fluidization velocity and transition velocity to fast fluidization, we choose appropriate operating conditions and demonstrated continuous solid circulation at high pressure condition (5 bar-abs) up to 24 hours.

Analytical, experimental and numerical study of timber-concrete composite beams for bridges

  • Molina, Julio C.;Calil, Carlito Junior;de Oliveira, Diego R.;Gomes, Nadia B.
    • Computers and Concrete
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    • v.24 no.2
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    • pp.103-115
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    • 2019
  • In this study, the strength and stiffness (EI) of wood-concrete composite beams for bridges with T-shaped cross section were evaluated. Two types of connectors were used: connectors bonded with epoxy adhesive and connectors attached to the wood just by pre-drilling (without adhesive). The connectors consisted of common steel bars with a diameter of 12.5 mm. Initially, the strength and stiffness (EI) of the beams were analyzed by bending tests with the load applied at the third point of the beam. Subsequently, the composite beams were evaluated by numerical simulation using ANSYS software with focus on the connection system. To make the composite beams, Eucalyptus citriodora wood and medium strength concrete were used. The slip modulus K and the ultimate strength values of each type of connector were obtained by direct shear tests performed on composite specimens. The results showed that the connector glued with epoxy adhesive resulted in better strength and stiffness (EI) for the composite beams when compared to the connector fixed by pre-drilling. The differences observed were up to 10%. The strength and stiffness (EI) values obtained analytically by $M{\ddot{o}}hler^{\prime}$ model were lower than the values obtained experimentally from the bending tests, and the differences were up to 25%. The numerical simulations allowed, with reasonable approximation, the evaluation of stress distributions in the composite beams tested experimentally.

Synthesis and Characteristics of Hard Coating Solution Using Colloidal Silica and Organic Silane through Sol-Gel Process (졸-겔 법에 의한 콜로이드 실리카와 유기 실란을 이용한 하드코팅 용액의 제조 및 특성)

  • Son, Dae Hee;Lee, Yun-Yi;Kim, Seong Jin;Hong, Seong-Soo;Lee, Gun-Dae;Park, Seong Soo
    • Applied Chemistry for Engineering
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    • v.22 no.6
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    • pp.691-696
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    • 2011
  • Organic-inorganic hybrid coating solutions were prepared by using a sol-gel method for transparent film. In this study, colloidal silica (CS) and alkoxy silane such as vinyltrimethoxysilane (VTMS), and [3-(methacryloyloxy)]propyltri methoxy silane (MAPTMS) were used in various conditions such as types of organic silane, weight ratios of CS to silane and reaction times. Coating solutions which were bar coated on the PET (polyethyleneterephthalate) film and cured were investigated on the chemical and physical properties. The pencil hardness and adhesion of coating films were increased with increasing the organic silane content in the coating solution compared to that of PMMA (polymethamethylcrylate) coating solution.