• 생명과학회지
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• 제21권1호
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• pp.96-101
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• 2011

ATP와 L-methionine으로부터 SAM synthetase (MetK)에 의해 생합성 되는 S-adenosylmethionine (SAM)은 세포내 메틸화에 필요한 메틸기를 제공하는 중심적인 공급체의 역할을 할뿐만 아니라 방선균에서는 일차 및 이차대사산물의 생산 조절에 관여하고 있다는 사실이 밝혀졌다. 이에 논 연구에서는 산업적으로 매우 중요한 항진균성 항생물질인 natamycin을 생산하는 S. natalensis로부터 SAM synthetase 코드하는 metK 유전자를 클로닝하고 동정하였다. S. natalensis에서 클로닝된 metK는 1,209 bp의 염기를 가진 유전자로써 아미노산서열에서 S. pristinaespiralis ATCC 25486과 S. peucetius ATCC 27952의 MetK와 96%, S. violaceusniger Tu 4113과 95% 일치하는 매우 높은 상동성을 보였다. 또 pSET152ET 벡터를 이용해 구축한 metK 고발현용 재조합 플라스미드 pCD1를 S. lividans TK24의 genomic DNA에 도입하여 actinorhodin 생산 유도를 시도해 본 결과 R5 고체배지에서 pCD1이 도입되지 않은 균주에서는 actinorhodin 생산을 전혀 확인할 수 없었지만 pCD1이 도입된 형질전환체에서는 actinorhodin 생산이 강하게 유도되었으며 R4 액체배지에서는 actinorhodin 생산량이 10배 증가되었다. 따라서 본 연구를 통해 클로닝된 S. natalensis 유래 metK 유전자는 방선균에서 이차대사산물의 생산을 유도할 수 있음을 확인할 수 있었다.

• 한국수소및신에너지학회논문집
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• 제27권4호
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• pp.462-469
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• 2016

This paper presents the results of thermodynamic cycle analysis and performance tests of alternative mixtures in low temperature applications. Two near-azeotropic binary mixtures R-152a/R-1270 (35:65 by wt.%) and R-290/E170 (35:65 by wt.%) are considered in this study. They have zero ODP (Ozone Depletion Potential) and much lower GWP (Global Warming Potential) than R-404A which is an alternative of R-502. Refrigeration cycle characteristics such as cooling capacity, coefficient of performance, suction and discharge pressures and temperatures are compared to those for the baseline refrigerants (R-502 and R-404A) cycles. The performance tests are conducted at the evaporation and condensation temperatures of $5^{\circ}C$ and $45^{\circ}C$, subcooling and superheating temperatures of $5^{\circ}C$, respectively. Performance comparisons between baseline and alternative refrigerants are conducted on the same cooling capacity. The system performance of newly proposed refrigerant mixtures show promising results.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.1264-1269
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• 2004

This research describes the application of hydrocarbon refrigerants for heat pump system which is needed for fish farm. Tested refrigerants are HCFC-22 and hydrocarbon refrigerants(CARE 50 and ASR-20). CARE 50 is mixture of R-290 and R-170, and ASR-20 is mixture of R-152a, R-290 and other additives. Heat pump consist of shell and tube heat exchanger, scroll compressor, expansion valve and accumulator. Manual expansion valve is used for testing of wide range evaporating temperature. Hydrocarbon refrigerants show a good performance as an alternative for HCFC-22 in the range of evaporating temperature from $-6^{\circ}C$ to $6^{\circ}C$.

• 한국경영과학회:학술대회논문집
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• 한국경영과학회 2008년도 추계학술대회 및 정기총회
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• pp.152-156
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• 2008

This study examines the relationships between R&D investment and Market value of Firms using data of small and medium enterprises (SMEs) in the manufacturing sector of Korea. In particular, this paper investigates the role of technology innovation system in the impact of R&D investment on firm's Market value of Firms. Findings from the previous studies on the relationship between R&D investment and Market value of Firms are positive relationship. Main of the previous studies demonstrated a positive impact of R&D investment on Market value of Firms. On the other hand, some recent studies showed this is not the case. Those studies persisted that the technological innovation system for managing and efficiently utilizing R&D investment and capability has to be built in order for R&D investment to give rise to increases in Market value of Firms. According to the Oslo manual by OECD, it is assumed that a technology innovation system can becharacterized as three factors :capability for technological innovation, capability for technology commercialization, capability for technological innovation management. This study divides sample firms into two groups using the "Inno-Biz" certificate system of the Korean Small and Medium Business Administration (SMBA): Inno-Biz firms Vs. Not Inno-Biz firms. The system selects innovative SMEs denoted as "Inno-Biz" using the above factors as criteria. The results revealed that the technology innovation system has the moderating effect to R&D investment on Market value of Firms.

• Transactions on Electrical and Electronic Materials
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• 제10권5호
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• pp.152-155
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• 2009

The microstructure and the electrical properties of a ZnO varistor, which was composed of a ZnO-$Bi_2O_3$-$Sb_2O_3$-CoO- $MnO_2$ -NiO-$Nd_2O_3$ system, were investigated at various $Y_2O_3$ addition concentrations. $Y_2O_3$ played a role in the inhibition of the grain growth. As the $Y_2O_3$ content increased, the average grain size decreased from $6.8{\mu}m$ to $4{\mu}m$, and the varistor voltage($V_{1mA}$) greatly increased from 275 to 400 V/mm. The nonlinearity coefficient ($\alpha$) decreased from 72 to 65 with increasing $Y_2O_3$ amount. On the other hand, the leakage current ($I_L$) increased from 0.2 to 0.9 ${\mu}A$. These results confirmed that doping the varistors with $Y_2O_3$ is a promising production route for production of a higher fine-grained varistor voltage ($V_{1mA}$) which can dramatically reduce the size of the varistors.

• 설비공학논문집
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• 제4권2호
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• pp.123-136
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• 1992

A preliminary thermodynamic design model of two-evaporator refrigerator/freezer system is constructed. This system is based on Lorentz-Meutzner cycle using refrigerant mixtures. This model screens alternative refrigerant (R32, R125, R143a, R22, R134a, R152a, R124, R142b, R123) mixtures to select the best performance-giving refrigerant mixtures and its composition for the system. Also, it estimates the effects of cooling temperatures of intercoolers, evaporator's area ratio, cooling load ratio on the performance of the system. The COP of the system ranges from 1.4 to 1.6, which is superior to that of the single evaporator system charged with R12 by 13% to 29%. Among 15 mixtures, R22/R123, R143a/R123, R32/R142b, and R32/R124 (in the order of high COP) are most recommendable. For the case of R22/R123, R22 mass fraction more than 0.5(Load Ratio=1.0) or 0.7(Load Ratio=0.33) is recomended in order to replace R12 without reduction in volumetric capacity when keeping the compressor as the same one. COP has the highest value with X(R22)=0.7 and 0.8, respectively. For the case of R143a/R123, in the similar manner, mass fraction of R143a is more than 0.5 or 0.6 while best performance occurs at X(R143a)=0.8. Higher temperature intercooler is more important for the performance of the system than lower temperature intercooler. The area ratio of evaporators is roughly proportional to load ratio of the evaporators.

• Journal of Advanced Marine Engineering and Technology
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• 제37권8호
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• pp.836-842
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• 2013

본 연구에서는 고효율 20 kW급 해양온도차 발전사이클을 새롭게 제안하고, 발전사이클의 성능이 최대가 되는 작동유체를 선정하기 위해서 단일냉매 15종과 혼합냉매 16종을 적용하여 발전사이클의 성능을 비교 분석하였다. 시스템 효율, 필요 냉매순환량, 그리고 TPP와 같은 인자를 사용하여 발전사이클의 특성을 분석하였다. 우선, 발전사이클의 효율 측면에서는 R32/R152a(87:13)이 가장 높으며, 필요 냉매 순환량은 R717이 가장 낮다. 그리고 논문에서 새롭게 제시한 TPP의 분석 결과에서는 R32/R134a 70:30이 가장 적합한 냉매로 나타났다. 이상의 결과로부터, 각각의 분석인자에 적합한 냉매는 그 목적에 따라서 다르다는 것을 확인할 수 있었다. 따라서 이러한 분석인자에 맞는 냉매의 선정이 필요하다.

• Journal of Microbiology
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• 제41권2호
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• pp.152-156
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• 2003

Through enrichment on two nutrient agars, 57 Thermus isolates were recovered from 15 hot spring samples taken from the Rehai geothermal area, Tengchong, China. Unique growth characteristics were observed when the strains were transferred from YIM14 medium to Thermus medium. Phylogenetic analysis showed that the 16S rDNA sequences of the isolates and clones from the Rehai geothermal area farmed a monophyletic group on the phylogenetic tree. A secondary structure comparison showed that their 16S rRNAs have unique secondary structure characteristics.

• Child Health Nursing Research
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• 제7권3호
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• pp.371-382
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• 2001

This study was conducted to investigate correlation theory of planned behavior, self efficacy and health locus of control about smoking cessation behavior for university student. We sampled 204 university student smokers using questionnaires and analyzed the data using Pearson correlation coefficient. The results of this study were as follows : 1. There were significant positive correlation between theory of planned behavior(intention : r=.215, p=.005, attitude : r=.160, p=.025, perceived behavior control : r=.298, p=.000) and self efficacy. 2. There were significant positive correlation between theory of planned behavior(attitude : r=.228, p=.002) and internal health locus of control. There were significant positive correlation between theory of planned behavior(attitude : r=.203, p=.004, subjective norm : r=.141, p=.047) and external health locus of control. There were significant negative correlation between theory of planned behavior(perceived behavior control : r=-.152, p=.034) and accidental health locus of control. This study has shown a correlation between theory of planned behavior and self efficacy and health locus of control. As a result of these findings, I suggest to develop the intervention program for smoking cessation.

• 대한기계학회논문집
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• 제15권6호
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• pp.2189-2205
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• 1991

본 연구에서는 혼합냉매의 열역학적 물성치를 정확하게 예측하기 위하여 척력 과 인력항으로 된 간단한 형태의 상태방정식을 택하고 이를 이용하여 순수성분에 대한 열역학적 물성치 자료와 혼합냉매에 대한 기액평형상태 자료를 이용하여 혼합물에 대 한 열역학적 물성치를 보다 더욱 정확하게 예측 할 수 있는 방법에 관해 연구하고자 한다.혼합냉매에 대한 상태방정식과 이상기체 상태의 비열자료를 기초로 열역학적 관계식을 이용하여, 압력-엔탈피, 온도-엔트로피 관계를 공식화하며, 혼합냉매에 대한 열펌프 및 냉동사이클 해석에 필요한 자료를 제시한다.