• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.207-214
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• 1996

To examine the developmental capacity of manipulated embryos after ultrarapid refreezing and thawing, mouse embryos were biopsied at 4-cell stage, frozen twice at 4-cell and morula stages, respectively, and then transferred to rec-ipients. Single blastomeres were biopsied from 4-cell embryos by a modified aspiration method. Biopsied 4-cell embryos were equilibrated into freezing medium at room temperature for 2.5 min, loaded into 40 $\mu$I of freezing medium in 0.25 ml plastic straw and then directly immersed into liqiud nitrogen. Freezing medium for 4-cell embryos consisted of 4.0 M ethylene glycol and O.25 M sucrose in dPBS supplemented with 6 mg/lm BSA. Morulae were frozen into freezing medium containing 5.0 M glycerol instead of ethylene glycol. Thawing was conducted by agitating each straw in 3TC water for 20 sec. The c content of each straw was expelled into 0.5 ml of dilution medium, which consisted of 0.25 M sucrose and 3 mg/ml BSA in dPBS. The thawed embryos were rehydrated in dilution medium for 10 min, washed 3 times with dPBS and then cultured in M16 medium at 37$^{\circ}C$, 5% CO$_2$ in air. Blastocysts that developed from frozen or refrozne biopsied embryos were transferred to recipients on Day 3 of pseudopregnancy, respectively. In vitro and in vivo developmental rates of the biopsied and intact 4~cell embryos after freezing and thawing were 78 (10l/130) and 25% (10/40), and 91 (114/125) and 30% (12/40), respectively. Although the rates of in vitro development of biopsied and intact embryos to blastocyst stage were significantly different after freezing and thawing (P

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.9
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• pp.1197-1204
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• 2012

As an effort to develop functional food ingredients and to discover the biological activity, the total phenolic content, total flavonoid content, DPPH and ABTS radical scavenging activity, SOD-like activity, ferric reducing antioxidant power (FRAP), and $Fe^{2+}$ chelating of Aster scaber were measured using a 70% ethanol extract and various solvent fractions. As a result, the total phenolic concent was highest in an ethyl acetate fraction of 141.9 mg GAE eq/g and the total flavonoid content was 105.6 mg QUE eq/g. The DPPH radical scavenging activity was highest in an ethyl acetate fraction of 97.1% at a concentration of 1,000 ${\mu}g/mL$ (p<0.05). The ABTS radical scavenging activity showed a 86.9% ethyl acetate fraction and a 57.9% butanol fraction at a concentration of 125 ${\mu}g/mL$, and higher than that of positive control (${\alpha}$-tocopherol and BHT) (p<0.05). The SOD-like activity showed 42.8% in an ethyl acetate at a concentration of 1,000 ${\mu}g/mL$. The ethyl acetate fraction showed the highest value of FRAP at 1051.9 ${\mu}M$ and a concentration of 1,000 ${\mu}g/mL$ (p<0.05). The $Fe^{2+}$chelating was highest in the 70.1% chloroform fraction at a concentration of 500 ${\mu}g/mL$ (p<0.05). There is the highest correlation between DPPH radical scavenging activity and FRAP (r=0.981) as compared to other antioxidant assays (p<0.01). With these results, we confirmed that the ethyl acetate fraction of Aster scaber has great antioxidant potential. So it can be expected to be developed into a specific functional food ingredient.

• Journal of Hospice and Palliative Care
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• v.5 no.2
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• pp.125-135
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• 2002

Purpose : The purpose of this study was to investigate the impact of depression, discomfort, spirituality, physical care, and opioid use on pain with terminally ill cancer patients in the hospice units. Method : The convenient sample of this study consisted of 58 terminally ill cancer patients at three hospice units in university-affiliated hospitals. Patients were interviewed with structured questionnaires. The data was analyzed using ANOVA, Pearson correlation coefficient, and multivariate multiple regression. Result : The results of this study were as follows : 1) The mean age of the participants was approximately 57 years. Regarding diagnosis, stomach cancer showed the highest frequency (24.1%), followed by lung cancer (17.2%) and rectal cancer (13.8%). Regarding motivation for admission to the hospice unit, the majority of the participants indicated pain control (67.2%), followed by spiritual care (39.7%), and symptom relief (27.6%). 2) The mean pain level measured by VAS was 5.13 (${\pm}2.61$). Regarding pain type, the highest pain frequency the participants experienced was deep pain (53.4%), followed by multiple pain (20.7%), intestinal pain (17.3%), and neurogenic (5.2%) and superficial pain (3.4%). 3) Regarding the factors influencing pain, the pain level was significantly affected by the depression level (P<0.01) and the opioid use (P<0.01). Conclusion, In summary, the higher the level of pain the terminally ill cancer patents had the higher the depression level as well as the opioid use. Thus, health care professionals need to continuously provide holistic care for them to die comfortably.

• Korean Journal of Ichthyology
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• v.28 no.2
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• pp.125-133
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• 2016

Sharks (Selachii) comprising about 510 valid species worldwide. Forty-three shark species belonging to 30 genera, 19 families and 8 orders have been found in Korean coastal waters, and all these are registered at the IUCN Red List as followings: endangered (EN) 1 species (2.3%) (vs. worldwide 15 spp., 3.2%), vulnerable (VU) 11 spp. (25.6%) (vs. 48 spp., 10.3%), near threatened (NT) 10 spp. (23.3%) (vs. 67 spp., 14.4%), least concern (LC) 9 spp. (20.9%) (vs. 115 spp., 24.7%), data deficient (DD) 12 spp. (27.9%) (vs. 209 spp., 44.9%), and critically endangered (CR) absent (vs. 11 spp., 2.4%). Twelve species among 43 sharks distributing in Korean waters are assessed as Threatened in the IUCN Red List categories and criteria as followings: Sphyrna lewini (EN, A2bd+4bd), Rhincodon typus (VU, A2bd+3d), Cetorhinus maximus (VU, A2ad+3d), Carcharodon carcharias (VU, A2cd+3cd), Isurus oxyrinchus (VU, A2abd+3bd+4abd), Alopias pelagicus (VU, A2d+4d), A. vulpinus (VU, A2bd+3bd+4bd), Carcharhinus plumbeus (VU, A2bd+4bd), S. zygaena (VU, A2bd+3bd+4bd), Squalus suckleyi (VU, A2bd+3bd+4bd), Squatina japonica (VU, A2d+4d), and S. nebulosa (VU, A2d+4d). Eighteen chondrichthyan species are registered in the list of the CITES Appendices, among them five sharks are distributing in Korean waters as Appendix II, i.e., R. typus, C. maximus, C. carcharias, S. lewini and S. zygaena. The scientific name of "Gobsangeo" has been changed from Squalus acanthias of the North Pacific to S. suckleyi, and "Mojorisangeo" from S. megalops of the Northwest Pacific to brevirostris.

• Journal of Embryo Transfer
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• v.16 no.2
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• pp.117-125
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• 2001

Vitrification of oocytes has been applied recently fur pigs, but remains elusive. The purpose of this study is to investigate the effects of vitrification in open pulled straws (OPS) on in vitro survival of porcine oocytes. When immature follicular oocytes frozen-thawed were cultured for in vitro maturation, maturation rates to metaphase-II stage were higher in oocytes with (25%) than without (15%) cumulus cells. After In vitro fertilization of oocytes frozen-thawed, the maturation rates were also significantly (P<0.05) higher in oocytes with (41%) that than without (17%) cumulus cells. However, the penetration rates were higher in oocytes without (19%) that than with (9%) cumulus. In another experiment, porcine oocytes matured in vitro were frozen and thawed for in vitro fertilization. The penetration rates were higher than in oocytes without (35%) that than with (26%) cumulus cells. However, the proportions of oocytes dead after in vitro fertilization were significantly (P<0.05) higher in oocytes with that than without cumulus cells. On the other hand, the rates of penetration and dead oocytes at 6 h after in vitro fertilization were not significant differences between oocytes with and without cumulus cells. However, the proportions of dead oocytes with (18%) and without (16%) cumulus cells were higher than in oocytes of control group (0%). These finding indicated the possible broader application for OPS, as they demonstrated that the maturation and fertilization in vitro by frozen-thawed oocytes may be accompained by cumulus cells and culture periods according to the requirements of the survival ability after freezing of mature and immature oocytes in pigs.

• The Korean Journal of Pain
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• v.1 no.1
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• pp.106-119
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• 1988

For the relief of pain in 3 cases of whiplash syndromes (case I, II and IV) and in one of reflex sympathetic dystrophy (case III), we have carried out six intentional. total spinal blocks (TSB) which attempted two times in case I, three in case II and one in carte III whoso various symptoms were chronically unresponsive to the usual conservative treatments, and a time of cervical epidural and right suprascapular nerve block in case W whose acute symptom lasted 4 drys following the cervical injury (see fables from 1 to 9). During the 753, we have observed clinically the sequential charges of respiration, lid and pupil reflexes, body motion and consciousness. And checked the blood pressure, pulse rate and arterial Pco2. The effectiveness of those blocks has been assessed by using the Visual Analog Scale which is designed to measure the patient$\acute{s}$ subjective intensity of pain and also we have found out the sequelae following those blocks. The methods of the blocks were as the following: 1. Under the N.P.O. for 8~10 hours, the preparations of immediate cardiopulmonary resuscitation and premedication with atropine 0.5mg at thirty minutes before the TSB, it was performed by injecting the mixture of 2% mepivacaine 10 or 15ml and normal saline 10 or 5ml through No. 23 G. spinal needle into the subarachnoid space of $C_7-T_1$ interspinous region with fully flexed neck on the lateral posture. Immediately after the injection of the local anesthetic in the lateral position, the patient$\acute{s}$ were hasten to change Trendelenburg$\acute{s}$ position in order to act the drugs cephalad and to make easy controlled respiration with oxygen. 2. The cervical epidural block was done by injecting the mixture of 0.5% bupivacaine 4ml, normal saline 4ml and triamcinolone 15mg through No. 18 G. Tuohy needle into the epidural space on the same region and posture as the above without premedication.7he suprascapular nerve block was done by injecting of 0.5% bupivacaine 3ml only into the right suprascapular fossa on the sitting posture. The results were as the following: 1. The cessation of respiration was seen within 5 minutes following the subarachnoidal injection of the above 20ml mixture in 2 to 3 minutes and then soon the consciousness began to disappear. The loss of Lid and pupil reflexes noted between 5 to 10 minutes and the size of the dilated pupils was equal between 5 to 20 minutes, but the pupil of the dependent side on tile lateral position was dilated 1 to 3 minutes earlier than that of the independent. The patients had r=ever responded to any stimulations during the TSB except their heart funtion. 2. The recovery of the TSB was as the following, firstly the ankle and lower limb of the independent side began to move slightly with in 34 to 75 minutes after the injection and then that of the dependent Secondly the neck and upper limb moved 6 to 15 minutes later than the lower limb. Thirdly the self respiration began to appear between 40 to 80 minutes from the block. The lid and pupil reacted to touch and light respectively between 40 to 80 minutes but the pupil of the independent side responded earlier than that of the depends. Lastly the consciousness recovered completely between 80 to 125 minutes from the block. 3. In the cardiopulmonary function during the TSB, the blood pressure were stable except the 210/130 tory at the and block of case I. There were bradycardias between 65 to 85 minutes in case I and II but no arrythmia on the EKG. The level of the arterial Pco2 was maintained to 43~45 torr during the TSB. 4. The effectiveness of the above blocks was no pain(0%) in case IV, and light (10~20%) in case I and II but no improvement in case III. 5. The right arm weakness has been complicated as to be Injected accidently the "COLD" local anesthetic at the End block of case I.

• The Korean Journal of Rehabilitation Nursing
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• v.3 no.1
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• pp.27-42
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• 2000

The purpose of this study was to identify the degree of sexual adjustment and quality of life in married men with spinal cord injuries and to furnish primary data to sexuality rehabilitation nursing practice which can lead to ideal sexual life and quality of life those men with SCI. Seventy married men with SCI were conveniently sampled in Kwangju, Suncheon, Mokpo and Seoul for responding the questionnaire, which was based on this research. With the research scale, SIS (Sexual interest and satisfaction) by Siosteen et al.(1990) and SB(Sexual behaviour) by Kreuter et al.(1996) were used for the measurement of sexual adjustment. And, for measuring quality of life was used SCI QL-23(Spinal cord injury of life-23) scale by Lundqvist et al.(1997). Data were collected from January 20 to March 20, 1999, using a structured questionnaire. A hundred volumes of questionnaire were used. and 85 volumes were collected, 70 volumes were used as research data after excluding 15 volumes unsuitable to data analysis. The obtain data were analysed using percentage, t-test, ANOVA, Duncan test, and Pearson's correlation by SAS PC+ program. The results were as follows: 1. The mean score of sexual interest and satisfaction of the subjects was 8.42. out of 18. The ways of their sexual behavior after spinal cord injuries were embracing and caressing (62.9%), kissing(58.6%), caressing breast with hands (55.7%), caressing breast with hands (52.9%), caressing genitals with hands (37.2%), caressing genitals with mouth(30.0%) and sexual intercourse (18.6%). The mean score of quality of life was 52.53 out of 100. 2. Age(F=3.24, p=.045) and caregiver (F=4.02, p=.022)were major variables which reveals significant differences in terms for sexual interest and satisfaction. The later results on Duncan's test showed that subjects who were in their 30s or 40s were significant higher than subjects whose age were in their 50s in their sexual interest and satisfaction. Also subjects with their spouse's care or mother's care were higher than those with other's care. 3. Subjects with incomplete paraplegia were higher than those with the complete paraplegia in sexual interest and satisfaction (F=3.01, p=.036). 4. Variables that showed the significant differences in the quality of life were education(t=2.860, p=.007) and period of marriage(t=2.125, p=.037). and occupational status(t=-2.161, p=.034). High school graduates, those who married before spinal cord injuries and those who didn't have occupation were higher than the other subjects. 5. Variables that revealed significant differences in the quality of life were time passage after spinal cord injuries(F=8.72, p=.001) and injured level of spinal cord(F=3.32, p=.042). Duncan's test showed that subjects who had lived for less than 4 years were higher those with time passage of 5-9 years and 10 years. Also subjects with lumbar injuries were higher than those with thoracic injuries in terms of quality of life. 6. There was negatively correlated between sexual interest and satisfaction and quality of life(r=-.256, p<.05). As a result of these findings sexuality rehabilitation for individuals with SCI was very important issue for their quality of life. Thus, registered nurses who care clients with SCI should activily participate in the client's sexual needs. Also, various sexual behaviors as well as sexual intercourse should be encouraged for the sexual adjustment of client's with SCI.

• Korean Journal of Agricultural Science
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• v.2 no.1
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• pp.125-130
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• 1975

This investigation was carried out to define the cultivation state of Ginseng seedling and to study the growth of important characters by location and correlations between characters, the results obtained are summarized as follows; 1. Most of farmers culitvating ginseng seedling had not any special consideration for the improvement of soil, and they applied green manure only as basic fertilizer but they did not apply chemical fertilizer. 2. Any efforts on the improvement of ginseng seed were not found. Seed was harvested from 3 years old ginseng plant. Seedling density was 1.8 cm by 2 cm. 3. Growth of all characters showed significant differences among farms. Coefficients of variation among individual seedling plants was largest at root diameter and smallest at root length. 4. Gromth of all characters without root diameter by planted location under the shadow was poor at front row, however root diameter was shortest at back row. 5. Significant high correlatons were rocognized between root diameter and stem diameter, between leaf blade and stem diameter and between leaf blade and leaf length, but stem length were not correlated with any characters. It is noticable that negative correlations were found between stem length and root diameter, between stem length and root length and between stem length and stem diameter.

• The Journal of Korean Academy of Prosthodontics
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• v.47 no.2
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• pp.125-135
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• 2009

Statement of problem: Loosening or fracture of the abutment screw is one of the common problems related to the dental implant. Generally, in order to make the screw joint stable, the preload generated by tightening torque needs to be increased within the elastic limit of the screw. However, additional tensile forces can produce the plastic deformation of abutment screw when functional loads are superimposed on preload stresses, and they can elicit loosening or fracture of the abutment screw. Therefore, it is necessary to find the optimum tightening torque that maximizes a fatigue life and simultaneously offer a reasonable degree of protection against loosening. Purpose: The purpose of this study was to present the influence of tightening torque on the implant-abutment screw joint stability with the 3 dimensional finite element analysis. Material and methods: In this study, the finite element model of the implant system with external butt joint connection was designed and verified by comparison with additional theoretical and experimental results. Four different amount of tightening torques(10, 20, 30 and 40 Ncm) and the external loading(250 N, $30^{\circ}$) were applied to the model, and the equivalent stress distributions and the gap distances were calculated according to each tightening torque and the result was analyzed. Results: Within the limitation of this study, the following results were drawn; 1) There was the proportional relation between the tightening torque and the preload. 2) In case of applying only the tightening torque, the maximum stress was found at the screw neck. 3) The maximum stress was also shown at the screw neck under the external loading condition. However in case of applying 10 Ncm tightening torque, it was found at the undersurface of the screw head. 4) The joint opening was observed under the external loading in case of applying 10 Ncm and 20 Ncm of tightening torque. 5) When the tightening torque was applied at 40 Ncm, under the external loading the maximum stress exceeded the allowable stress value of the titanium alloy. Conclusion: Implant abutment screw must have a proper tightening torque that will be able to maintain joint stability of fixture and abutment.

• Research in Community and Public Health Nursing
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• v.12 no.1
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• pp.125-141
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• 2001

The purpose of this study was to investigate the factors affecting hearing loss in adolescent's use of PCPs, and to give the basic data for adolescent's hearing conservation program development and prevention education against their hearing loss. This study was a descriptive research about three factors affecting hearing loss; the knowledge and attitude about noise, the perception of hearing loss and the hearing threshold. The subjects of this study were 383 students in two general high schools and two vocational high schools in Teagu. They have been using PCPs but with no current or past ear disease. This study was carried out from Sep. 1. 2000 to Oct. 24, 2000. The instrument used for the knowledge and attitude about noise was a questionnaire developed by Rhee. Kyung Yong and Yi. Kwan Hyung(1996). The instrument used for the perception of hearing loss was a Smith Hearing Screening Questionnaire. A Belton Model 112 Audiometer. air-conduction hearing test instrument. was used for the hearing threshold. Data was analysed by a SPSS/Win 10.0 program with frequency. percentage, t-test. ANOVA and pearson correlation. The results of this study were as follows; 1. The average of concern about hearing scored the highest $3.66{\pm}0.70$. The average of perceived susceptibility scored $2.64{\pm}0.85$ and the average of knowledge about noise scored $2.13{\pm}0.56$. The average of total knowledge and attitude about noise scored $2.82{\pm}0.46$. The average of discomfort of hearing loss($2.51{\pm}0.81$) scored higher than that fear of hearing loss($1.35{\pm}0.53$). The average of total perception of hearing loss scored $1.93{\pm}0.59$. The hearing threshold of the subjects scored the highest at 500Hz(Lt. $23.21{\pm}6.62$, Rt. $23.39{\pm}7.02$) and scored higher in order of 1000Hz, 2000Hz, 4000Hz and 8000Hz. 2. The knowledge and attitude about noise and the perception of hearing loss were both affected only by one important characteristic, which was general and vocational high schools. The knowledge and attitude about noise raked (t=5.258, p=0.000), and perception of hearing loss raked(t=2.241. p=0.026). However. several other important characteristics also impacted significantly on the knowledge and attitudes about noise. They included grade (t = 1. 987. p=0.048), father's education(F=2.745. p=0.043), marks(F=3.157, p=0.044), drinking(t=2.307, p=0.022) and smoking(t=2.587, p=0.010). The left hearing threshold differed significantly by sex at 1000Hz(t=5.175, p<0.001) and 8000Hz (t=3.334, p<0.01). According to general and vocational high schools (p<0.001), at 500Hz (t=-5.056), 1000Hz (t=-5.253), 2000Hz (t=-4.905), 4000Hz (t=-4.704) and 8000Hz (t=-5.204) significant differences were also shown. Marks were significant at 1000Hz (F=3.824, p<0.05) and drinking was found to be significant at 500Hz(t=2.203, p<0.05). The right hearing threshold differed significantly by sex at l000Hz(t=5.557. p<0.001). 4000Hz(t=2.234. p<0.05) and 8000Hz (t=2.730. p<0.01). According to general and vocational high schools(p<0.001) at 500Hz (t=-4.730), 1000Hz(t=-6.271). 2000Hz (t=-4.573). 4000Hz(t=-3.554) and 8000Hz (t=-3.405) significant differences were also shown. Grades impacted at 500Hz(t=2.201. p<0.05) and 4000Hz(t=2.511. p<0.05), while marks were significant at l000Hz(F=4.1l5. p<0.05) and drinking was significant at 500Hz(t=2.333. p<0.05). 3. The left hearing threshold in accordance with use of PCPs differed significantly at 2000Hz(F=2.996. p=0.03l) according to volume level and at 8000Hz(F=2.197. p=0.022) according to duration${\times}$hours per day. The right hearing threshold differed significantly at l000Hz(F=3.075. p=0.028) according to volume level and at 8000Hz(F=2.925. p=0.034) according to duration. 4. The knowledge and attitudes about noise showed a light positive correlation with the perception of hearing loss. A positive correlation was shown. as stated previously in all Hz, between the left hearing threshold and the right hearing threshold, especially the highest correlation at 2000Hz(r=0.761. p=0.000). This study has shown that the factors related to adolescent's use of PCPs are important as they impact significantly an adolescent's hearing. These results then indicate that in future, when designing a hearing conservation program and prevention education this data should be considered.