• Title/Summary/Keyword: R&BD

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DNA Dynamics: a Fluorescence Resonance Energy Transfer Study Using a Long-Lifetime Metal-Ligand Complex

  • Kang, Jung-Sook;Lakowicz, Joseph-R.;Piszczek, Grzegorz
    • Archives of Pharmacal Research
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    • v.25 no.2
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    • pp.143-150
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    • 2002
  • Fluorescent probes bound to DNA typically display nanosecond decay times and reveal only nanosecond motions. We extend the time range of measurable DNA dynamics using $[Ru(pby)_2(dppz)]^{2+}$ (bpy=2.2'-bipyridine, dppz=dipyrido[3,2-a2',3'-c]phenazine) (RuBD) which displays a mean lifetime near 90 ns. To test the usefulness of RuBD as a probe for diffusive processes in calf thymus DNA, we compared the efficiencies of fluorescence resonance energy transfer (FRET) using three donors which display lifetimes near 5 ns for acridine orange (AO), 22 ns for ethidum bromide (EB) and 92 ns for RuBD, with nile blue (NB) as the acceptor. The F rster distances for AO-NB, EB-NB and RuBD-NB donor-acceptor pairs were 42.3, 52.3, and $30.6{\;}{\AA}$, respectively. All three donors showed dramatic decreases in fluorescence intensities and more rapid intensity decays with increasing NB concentrations. The intensity decays of AO and EB in the presence of varying concentrations of NB were satisfactorily described by the one-dimensional FRET model without diffusion (Blumen and Manz, 1979). In the case of the long-lifetime donor RuBD, the experimental phase and modulation somewhat deviated from the recovered values computed from this model. The recovered NB concentrations and FRET efficiencies from the model were slightly larger than the expected values, however, the recovered and expected values did not show a significant difference. Thus, it is suggested that the lifetime of RuBD is too short to measure diffusive processes in calf thymus DNA.

Methodology for Deriving Required Quality of Product Using Analysis of Customer Reviews (사용자 리뷰 분석을 통한 제품 요구품질 도출 방법론)

  • Yerin Yu;Jeongeun Byun;Kuk Jin Bae;Sumin Seo;Younha Kim;Namgyu Kim
    • Journal of Information Technology Applications and Management
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    • v.30 no.2
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    • pp.1-18
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    • 2023
  • Recently, as technology development has accelerated and product life cycles have been shortened, it is necessary to derive key product features from customers in the R&D planning and evaluation stage. More companies want differentiated competitiveness by providing consumer-tailored products based on big data and artificial intelligence technology. To achieve this, the need to correctly grasp the required quality, which is a requirement of consumers, is increasing. However, the existing methods are centered on suppliers or domain experts, so there is a gap from the actual perspective of consumers. In other words, product attributes were defined by suppliers or field experts, but this may not consider consumers' actual perspective. Accordingly, the demand for deriving the product's main attributes through reviews containing consumers' perspectives has recently increased. Therefore, we propose a review data analysis-based required quality methodology containing customer requirements. Specifically, a pre-training language model with a good understanding of Korean reviews was established, consumer intent was correctly identified, and key contents were extracted from the review through a combination of KeyBERT and topic modeling to derive the required quality for each product. RevBERT, a Korean review domain-specific pre-training language model, was established through further pre-training. By comparing the existing pre-training language model KcBERT, we confirmed that RevBERT had a deeper understanding of customer reviews. In addition, all processes other than that of selecting the required quality were linked to the automation process, resulting in the automation of deriving the required quality based on data.

Optical Memory Disc Using Super-resolution Phenomenon of Super-RENS Technology

  • Kim, Ju-Ho;Hwang, In-O;Kim, Hyeon-Gi;Yun, Du-Seop;Park, In-Sik
    • Proceedings of the Optical Society of Korea Conference
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    • 2004.02a
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    • pp.248-251
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    • 2004
  • 근접장 초해상 기술 중 대표적인 Super-RENS (Super Resolution Near Field Structure) 기술을 이용하여 25GB 용량의 BD(Blu-ray Disc) 다음 세대로 예상되는 100 ${\sim}$ 200 GB 용량의 Media 및 기록 재생 기기를 개발하고자 한다. 현재까지 이 기술의 어려움이었던 Stability 특성은 10만회 재생 내구성을 확인하엿고 CNR (신호 대 잡음 비) 특성은 50 nm 미세 마크에 대하여 40 dB의 특성을 확보 하였다. 이 특성은 대략 75 GB에 대응하는 것으로 BD System을 그대로 사용하면서 달성한 세계 최고의 기록 밀도로 평가된다. 향후 과제는 Jitter. Error Rate등 실제 신호 특성의 확보로서, 이의 확보를 통하여 기술의 상품화 가능성이 확인될 것이다.

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Validation of the Korean Version of the Biological Rhythms Interview of Assessment in Neuropsychiatry

  • Cho, Chul-Hyun;Jung, Seo-Yeon;Kapczinski, Flavio;Rosa, Adriane R;Lee, Heon-Jeong
    • Psychiatry investigation
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    • v.15 no.12
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    • pp.1115-1120
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    • 2018
  • Objective The Biological Rhythms Interview of Assessment in Neuropsychiatry (BRIAN) is a scale used to clinically evaluate disturbances in biological rhythm. In this study, we aimed to examine the reliability and validity of the Korean version of the BRIAN (K-BRIAN) in a Korean population. Methods A total of 181 participants, including 141 outpatients with bipolar disorder (BD; type I, 62; type II, 79) and 40 controls, were recruited. Construct validity was tested by comparing the mean K-BRIAN scores of the BD patients and control subjects. Concurrent validity was tested by evaluating the association between the K-BRIAN and the Morningness-Eveningness Questionnaire (MEQ). Results The mean K-BRIAN scores of the control subjects and patients with BD differed significantly (p<0.001). Particularly, the mean K-BRIAN score was considerably lower among control subjects (mean${\pm}$standard deviation=$35.00{\pm}8.88$) than among patients with BD type I ($41.19{\pm}12.10$) and type II ($50.18{\pm}13.73$). The Cronbach's alpha for the K-BRIAN was 0.914. The K-BRIAN was found to correlate with the MEQ (r=-0.45, p<0.001). Conclusion The findings affirm that the K-BRIAN has good construct validity and internal consistency. This suggests that the K-BRIAN can be used to assess biological rhythms in the Korean population, especially for patients with mood disorder.

Analysis of Corporate R&D Capability with Industrial's Innovation Trend (산업별 기술혁신패턴에 따른 기업의 R&D 역량 비교 연구)

  • Shon, Hee-Jeon;Park, Mun-Su
    • Journal of Information Technology and Architecture
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    • v.10 no.1
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    • pp.47-62
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    • 2013
  • In this paper, we analyze the comparative advantage of industry innovation (R & D) activities. The method is that companies are classified suppliers dominated- industry, productionintensive industries and science-based industries, and data of Statistical analysis were collected HCCP (KRIVET). The result is that Tipping phenomena of science-based is apparent and suppliers dominated- industry is the lack of comparative advantage. The implications are as follows. suppliers dominated- industries that specialize in R & D capabilities, support R & D capability is required. Second, the policy in terms of support for R & BD (linking technology commercialization support innovation) should be strengthened. Third, SMEs in the leveling down of industry R & D capabilities should be supplemented.

Refractive Indexes of Porous Thin Films Prepared From Organic-templated Polymethylsilsesquioxanes

  • Kim, Jung-Soo;Nam, Dae-Geun;Oh, Weontae
    • Transactions on Electrical and Electronic Materials
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    • v.15 no.6
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    • pp.305-308
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    • 2014
  • Organosilyl-modified and star-shaped poly (${\varepsilon}$-caprolactone) (m-PCL) was prepared, and added to polymethylsilsesquioxane (PMSSQ), to make composites. The end groups of m-PCL are chemically similar to PMSSQ, and m-PCL mixed well with PMSSQ in the composite. Porous PMSSQ film was made by further calcination of the composite film at elevated temperature. m-PCL-templated PMSSQ and the as-prepared porous PMSSQ were structurally, optically, and thermally characterized in thin films. The chemical binding of m-PCL and PMSSQ effectively suppressed the phase separation of PMSSQ and m-PCL during the curing process. After calcination at elevated temperature, there remained many pores in the PMSSQ matrix. The refractive indices of the resulting porous PMSSQ thin films decreased with increase of the film porosities, depending on the initial m-PCL loadings.

A Optical System Design of LED Marine Lanterns Based on a TIR Collimator Lens (전반사 렌즈를 이용한 LED 등명기 광학계 설계)

  • Go, Dong Hyun;Lee, Yoon Chul
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.29 no.11
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    • pp.1-5
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    • 2015
  • In this paper, we propose the optical system design for a medium sized LED marine lanterns which simplifies the multi-layer structure into a single structure. In order to satisfy the target fixed intensity(35,000cd) and vertical divergence($-2.5^{\circ}{\sim}-4.0^{\circ}$, $+2.5^{\circ}{\sim}+4.0^{\circ}$), we use the total internal reflection collimator lens. And a Monte Carlo simulation has been utilized to optimize a condition of a LED package, TIR lens and outside lens. The computer simulation results indicated that this LED marine lanterns can produce of a fixed intensity(35,382cd) and vertical divergence($-3.1^{\circ}{\sim}+2.5^{\circ}$). Using the this optical system, we achieve the target value of LED lanterns.

Cloning of ori region of R-plasmid pSBK203 and construction of new shuttle-vectors for E. coli & B. subtilis using cloned fragments (R-plasmid pSBK203의 ori 부위 재조합 및 이를 이용한 E.coli와 B.subtilis 간의 Shuttle-Vector 구성)

  • 권동현;석종성;변우현
    • Korean Journal of Microbiology
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    • v.25 no.4
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    • pp.262-273
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    • 1987
  • The replication region of the chloramphenical resistance plasmid pSBK203 of Staphylococcus aureus was cloned using pBR322 and pBD9 as vectors. Cloned replication tegion and chloramphenicol resistance gene were recombined to pBR322. The reconstructed vector behaved as a shuttle vector for E. coli and B. subtilis.

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