• Title/Summary/Keyword: Quinones

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Optimization of Protein Extraction for Lichen Thalli

  • Kondratiuk, Anna S.;Savchuk, Oleksiy M.;Hur, Jae-Seoun
    • Mycobiology
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    • v.43 no.2
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    • pp.157-162
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    • 2015
  • Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buffer containing polyvinylpolypyrrolidone, ascorbic acid, Triton X-100, polyethylene glycol, proteinase, and oxidase inhibitors in sodium phosphate buffer was developed. This extraction buffer showed high efficiency for all lichen species tested in the study.

Characterization of Microbial Community in Biological Wastewater Treatment System Using Respiratory Quinone Profiles

  • Lim Byung-Ran;Ahn Kyu-Hong;Lee Yonghun
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2003.05a
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    • pp.111-114
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    • 2003
  • The dynamics of microbial community structure of the various domestic wastewater treatment processes were examined using a novel approach of quinone profiles. The compositions of microbial quinone of 5 sites fer plant and lab-scale activated sludge were analyzed. More than 14 kinds of quinones were observed in the activated sludges tested in this study. The microbial community structure of the plant activated sludge processes a little differed from that of the lab-scale submerged MBR systems. The dominant quinones were UQ-8, UQ-10 followed $MK-8(H_4)$, MK-7 and MK-6. The molar ratio of ubiquinones to menaquinones (UQ/MK) changed from 0.81 to 1.9, indicating that aerobic bacteria dominated the microbial community of the activated sludge examined. The microbial diversity of the activated sludges calculated from the all quinone compositions was 9.5-11.9 and the microbial equability of the activated sludges was 0.64-0.79.

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The Electrochemical Reaction Mechanism and Applications of Quinones

  • Kim, R. Soyoung;Chung, Taek Dong
    • Bulletin of the Korean Chemical Society
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    • v.35 no.11
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    • pp.3143-3155
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    • 2014
  • This tutorial review provides a general account of the electrochemical behavior of quinones and their various applications. Quinone electrochemistry has been investigated for a long time due to its complexity. A simple point of view is developed that considers the relative stability of the reduced quinone species and the values of the first and second reduction potentials. The 9-membered square scheme in buffered aqueous solutions is explained and semiquinone radical stability is discussed in this context. Quinone redox reaction has also been employed in various studies. Diverse examples are presented under three broad categories defined by the roles of quinone: molecular tool for physical chemistry, versatile electron mediator, and charge storage for energy conversion devices.

Lipid analysis of streptomycetes isolated form volcanic soil

  • Kim, Seung-Bum;Kim, Min-Young;Seong, Chi-Nam;Ouk, Kang-Sa;Hah, Yung-Chil
    • Journal of Microbiology
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    • v.34 no.2
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    • pp.184-191
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    • 1996
  • The cellular fatty acids and quinones of streptomycetes isolated from volcanic soils were analysed. The strains contained fatty acids of 14 to 17 carbon chains, and 12-methyltetradecanoic acid and 14 methylpentadecanoic acid were dominant in most strains. The total profiles consisted of 74% branched fatty acid family, 16.8% linear family and 8.2% unsaturated family. The largest cluster of grey spore meases defined by numerical classification was separated from the remainders in the principal component analysis, but the other clusters were overlapped with one another. In the analysis of respiratory quinones, all of the strains contained either the menaquinone of 9 isoprene units with 6 hydrogenations of 8 hydrogenations as the major species. The distribution of menaquinones among the clusters could provide an important key in the chemotaxonomy of streptomycetes.

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Effects of Aloe Extract on Ethanol Metabolism (알로에 추출물이 알콜대사에 미치는 영향)

  • 정정철;이주영;김미정;정진호
    • Journal of Food Hygiene and Safety
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    • v.11 no.1
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    • pp.31-34
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    • 1996
  • Aloe, being used widely as a health food and also as a traditional folk remedy for burns and constipation, contains quinone derivatives particularly in its skin. Thus, we have investigated the effect of extracts of Aloe in ethanol metabolism. The dried powder of water extract of skinned Aloe (300 mg/kg body weight given to rats by oral adminstration at 30 min prior to oral adminstration of ethanol given at a does of 4 gm/kg) and the freeze-dried Aloe gel commercial product (600 mg/kg) which was prepared after selective elimination of quinones were found not to increase the ethanol metabolism rate in vivo. This result suggested that quinones, missing from the above preparations, might be responsible for enhancing ethanol metabolism rate.

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Isoprenoid Quinone Profiles of the Leclercia adecarboxylata KCTC $l036^T$

  • Shin, Yong Kook;Jung Sook Lee;Chang Ouk Chun;Hong Joong Kim;Yong Ha Park
    • Journal of Microbiology and Biotechnology
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    • v.6 no.1
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    • pp.68-69
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    • 1996
  • The isoprenoid quinone composition of Leclercia adecarboxylata KCTC $1036^T$ was determined by using high-performance liquid chromatography. L. adecarboxylata KCTC $1036^T$ are characterized by their production of both ubiquinone-7, ubiquinone-8 and menaquinone-8 as major quinones. It is clear that the analysis of isoprenoid quinone profiles provides a new criterion of great promise for identifying Leclercia strains.

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