• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.68-68
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• 2005

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.69-70
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• 2005

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2002년도 총회 및 추계학술발표회
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• pp.126-126
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• 2002

• 식물병연구
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• 제25권4호
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• pp.233-236
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• 2019

2017년, 감귤류에 Citrus vein enation virus (CVEV)의 발생보고가 있었다. 식물방역법상 관리급 병원체인 CVEV에 대한 검역조치를 위해 감귤의 주요 재배지인 제주도를 포함한 4개도 29개 시군에서 온주밀감, 유자, 만숙성 감귤류(한라봉, 천혜향, 레드향, 황금향) 재배과원 등 203개소에서 시료를 수집하고 reverse transcription polymerase chain reaction (RT-PCR) 방법과 시퀀싱을 통해 진단하였다. 진단결과, 양성반응을 보인 시료를 대상으로 외피단백질 유전자 부위를 분석하기 위해 GenBank에 등록된 분리주들과의 염기서열과 아미노산 서열을 비교한 결과, 98% 이상의 매우 높은 상동성을 확인하였다. 전체 시료에 대하여 RT-PCR 진단 결과, 136개 과원(67%)에서 CVEV가 검출되었으며, 유자 과원의 85.4%, 온주밀감 과원의 77.8%가 CVEV에 감염된 것으로 파악됐다. 또한, 감귤의 주요 재배지인 제주도에서 90.6%의 과원이 CVEV에 감염된 것으로 확인하였다. 기주별·지역별 발생실태 조사를 토대로 국내 감귤류에 CVEV가 만연되어 있음을 확인하였다. 본 연구를 바탕으로, CVEV의 발생실태 조사와 검역병원체 여부를 검토하여 2018년 5월 CVEV를 비검역 병원체로 변경하였다.

• 한국동물위생학회지
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• 제42권4호
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• pp.289-296
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• 2019

A novel rapid procedure with liquid chromatography tandem mass spectrometry (LC-MS/MS) detection has been developed by changing various conditions including sample preparation such as QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology. This work has been involved the optimization and validation of detection method for fluoroquinolones which are widespread used in livestock especially in the chicken. Five grams of homogenized chicken muscle were extracted with QuEChERS EN and acetonitrile containing 5% formic acid and cleaned with anhydrous magnesium sulfate and C₁₈ sorbent. The separation was performed on Acquity UPLC HSS T3 (2.1 mm×100 mm, 1.8 ㎛) column. The mobile phase A and B were composed of water containing 0.1% formic acid and acetonitrile containing 0.1% formic acid, respectively. Flow rate was 0.25 mL/min and column temperate was 40℃. LC-MS/MS with multiple reaction monitoring has been optimized for ten fluoroquinolones (ciprofloxacin, danofloxacin, difloxacin, enrofloxacin, marbofloxacin, norfloxacin, ofloxacin, orbifloxacin, pefloxacin and sarafloxacin). The method developed in this study has been presented good linearity with correlation coefficient (R²) of 0.9971~0.9998. LOD and LOQ values ranged from 0.09 to 0.76 ppb and from 0.26 to 2.29 ppb, respectively. The average recoveries were from 77.46 to 111.83% at spiked levels of 10.0 and 20.0 ㎍/kg. Relative standard deviation (%) ranged 1.28~11.90% on intra-day and 3.10~8.38 % on inter-day, respectively. This analysis method was applicable to the livestock residue laboratories and was expected to be satisfactory for the residue surveillance system.

• 한국가금학회지
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• 제31권2호
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• pp.119-128
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• 2004

Highly Pathogenic Avian Influenza (HPAI) is a very acute systemic disease in poultry, particularly in chickens and turkeys caused by HPAI viruses. An outbreak of HPAI caused by subtype H5N1, was first reported in a broiler breeder farm on December 10, 2003 in Korea, although there had been twenty one outbreaks of the disease reported in the world before. Since mid-December 2003, eight Asian countries have confirmed outbreaks of HPAI due to the same subtype. The outbreak has also resulted in at least twenty three fatal human cases in Vietnam and Thailand as of May 17, 2004 according to the WHO. Regarding the first outbreak of recent Asian HPAI, it has been suspected that some Asian countries with the exception of Korea and Japan veiled the fact of HPAI outbreaks since the last half of 2003, even though it was first reported in Korea. There have been total nineteen outbreaks of HPAI among chicken and duck farms in 10 provinces in Korea since Dec. 2003 and approximately 5,280,000 birds were slaughtered from 392 farms for eradication of the disease and preemptive culling. The origin of the H5Nl HPAI virus introduced into the country are unknown and still under epidemiological investigation. Current status of outbreaks and characteristics of HPAI will be reviewed and discussed on the basis of genetic, virological, clinicopathological, and ecological aspect, as well as future measures for surveillance and prevention of the disease in Korea.

• 대한수의학회지
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• 제60권4호
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• pp.195-202
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• 2020

Feline calicivirus (FCV) infection results in a common upper respiratory disease associated with oral ulceration in cats. Although FCV infection has been reported in cats worldwide, the biologic and genetic features of South Korean FCV are unclear. We aimed to investigate the biological and genetic features of South Korean FCV isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate FCV from 58 organ homogenate samples. The FCV isolates were confirmed by cytopathic effects, immunofluorescence, electron microscopy, and reverse transcription polymerase chain reaction assays. Viral genetic analysis was carried out with VP2 gene and complete genomes of FCVs. Five viruses propagated in CRFK cells were confirmed to be FCVs. The FCV17D283 isolate showed the highest viral titer of 107.2 TCID50/mL at 36 h post-inoculation. Korean FCV isolates did not grow well in Vero, BHK-21, A72, or Madin-Darby canine kidney cells. The FCV17D03 and FCV17D283 isolates had the highest genetic similarity (80.1% and 86.9%) with the UTCVM-H1 and 14Q315 strains, which were isolated in the United States and South Korea in 1995 and 2014, respectively. We isolated five FCVs from cats and detected important genetic differences among them. FCV isolates did not show any virulent effects in mice.

• Journal of Veterinary Science
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• 제21권5호
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• pp.64.1-64.14
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• 2020

Background: Canine distemper virus (CDV) infection results in high morbidity and mortality in dogs. There has been no report about Isolation of Korean CDV since 1980 in Korea. Objectives: To investigate the biological properties and the genetic characterization of Korean CDV. Methods: Vero cells expressing dog signaling lymphocyte activation molecule (dSLAM) gene named as Vero/dSLAM were used to isolate CDV using 17 samples. Diagnostic methods such as cytopathic effects, immunofluorescence assay, peroxidase linked assay, electron microscopy, rapid immunodiagnostic assay, and reverse transcription polymerase chain reaction were used to confirm the Korean CDV isolate as a CDV. The genetic analysis was performed through cloning and sequencing of hemagglutinin gene of CDV isolate. Results: A virus propagated in Vero/dSLAM cell was confirmed as CDV (CD1901 strain) based on the above methods. The CD1901 strain showed the highest viral titer (10^5.5 50% tissue culture infectious dose [TCID₅₀]/mL) in the Vero/dSLAM cells at 4 days post inoculation, but did not form a fork on chorioallantoic membrane of 7-day-old egg. Ribavirin, a nucleotide analogue anti-viral agent, inhibits moderately the Korean CDV propagation in the Vero/dSLAM cells. The nucleotide and amino acid sequences of the H gene of CD1901 strain were compared with those of other CDV strains. The CD1901 strain belonged to Asia 1 group and had the highest similarity (99.9%) with the BA134 strain, which was isolated in China in 2008. Conclusions: We constructed successfully Vero/dSLAM and isolated one Korean CDV isolate (CD1901 strain) from a naturally infected dog. The CD1901 strain belonged to Asia 1 genotype.

• 한국응용곤충학회지
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• 제62권3호
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• pp.131-137
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• 2023

호박꽃과실파리(Z. scutellata)는 박과작물에 피해를 주는 중요한 해충이다. 현재 큐루어(Cuelure)는 국내에서 검역 대상 해충으로 규정된 오이과실파리, 타우과실파리, 및 퀸슬랜드과실파리와 더불어 호박꽃과실파리 수컷 성충의 예찰에 다양한 제형으로 활용되고 있다. 본 연구는 액체, 고체, 및 왁스제형의 큐루어에 대한 호박꽃과실파리 수컷 성충의 야외 유인효과를 검증하기 위한 것으로 2021년 4월부터 10월까지 제주 2개 지역과 경북 1개 조사에서 2주 간격으로 모니터링하였다. 호박꽃과실파리의 발생밀도가 높은 제주지역에서는 7월부터 8월에 액체와 왁스제형의 큐루어에 호박꽃과실파리가 더 많이 유인되어 통계적인 유의차가 있었으나, 호박꽃과실파리의 발생밀도가 낮은 경북지역에서는 제형별 유인력에 차이가 없었다. 야외조사 결과를 바탕으로 액체와 왁스제형의 큐루어가 호박꽃과실파리 수컷 성충 예찰에 권장되며, 이 두 제형의 큐루어에 유인되는 검역 대상 과실파리류의 국내 예찰에 효과적일 수 있다.

• 한국임상수의학회지
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• 제28권4호
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• pp.348-351
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• 2011

The purpose of this study was to determine the distribution of gram-negative bacteria isolated from companion animals with sepsis, and to investigate the antimicrobial susceptibility patterns of the isolates. Bacterial pathogens were isolated from specimens of dogs and cats submitted to National Veterinary Research and Quarantine Service between 2008 and 2009. A total of 44 gram-negative pathogens were isolated from necropsied organs. The most common isolates were E. coli (n = 33), K. pneumoniae (n = 4) and B. bronchiseptica (n = 4). Most of gram-negative isolates were susceptible to ceftiofur (68.2%), colistin (84.1%), florfenicol (84.1%) and spectinomycin (61.4%). Most of those were resistant to ampicillin (77.3%), erythromycin (86.4%), flumequine (65.9%), lincomycin (97.7%), oxytetracycline (61.4%), penicillin (100%), streptomycin (63.6%), spiramycin (97.7%), sulfamethoxazole (90.9%), tylosin (97.7%) and tiamulin (100%). In conclusion, colistin and florfenicol could be useful against sepsis due to gram-negative bacteria.