• Proceedings of the Korean Statistical Society Conference
• /
• 한국통계학회 2003년도 추계 학술발표회 논문집
• /
• pp.325-329
• /
• 2003

한우 6번 염색체 유전자 지도에서 QTL (quantitative trait loci) 분석을 실시하여 선별된Locus 값을 순열검정(Permutation Test)을 이용하여 유의성 검정을 실시하였다. 한편, 우수경제형질 DNA marker들을 K-평균 군집법을 실시 파악하였다. 이들 QTL과 K-평균법에 의해 한우의 염색체 6번 ILSTS035의 우수 DNA marker 235번을 선별하였다. 선별된 DNA Marker 235번을 출품우에 적용하여 Bootstrap 방법을 이용하여 신뢰구간을 구하여 검정하였다.

• Korean Journal of Poultry Science
• /
• 제22권2호
• /
• pp.97-104
• /
• 1995

Recently, DNA fingerprinting has been utilized as the most powerful tool for genetic analysis and improvement of poultry. This technique enables us to solve several problems of poultry breeding ; traits of low heritability, difficulty in keeping the performance records, measuring in late of life, and sex limited traits. Application of DNA fingerprinting is chiefly focused to individual and population identification, evolution force, quantitative trait marker, introgression of new gene, and prediction of heterosis. Thus, research work on DNA fingerprinting will he accelerated to analyze genetic components exactly and improve the performance of poultry.

• Communications for Statistical Applications and Methods
• /
• 제11권3호
• /
• pp.657-668
• /
• 2004

Permutation test has been applied for the QTL(quantitative trait loci) analysis and we selected a major locus. K -means clustering analysis, for the major DNA Marker mining of ILSTS035 microsatellite loci in Hanwoo chromosome 6, has been described. Finally, bootstrap testing method has been adapted to calculate confidence intervals and for finding major DNA Markers.

• Asian-Australasian Journal of Animal Sciences
• /
• 제12권7호
• /
• pp.1129-1134
• /
• 1999

The rapid development of the sheep genetic linkage map over the last five years has given us the ability to follow the inheritance of chromosomal regions. Initially this powerful resource was used to find markers linked to monogenic traits but there is now increasing interest in using the genetic linkage map to define the complex of genes that control multigenic production traits. Of particular interest are those production traits that are difficult to measure and select for using classical quantitative genetic approaches. These include resistance to disease where a disease challenge (necessary for selection) poses too much risk to valuable stud animals and meat and carcass qualities which can be measured only after the animal has been slaughtered. The goal for the new millennium will be to fully characterise the genetic basis of multigenic production traits. The genetic linkage map is a vital tool required to achieve this.

• Journal of the Korean Data and Information Science Society
• /
• 제25권5호
• /
• pp.1127-1135
• /
• 2014

Genome-wide association studies (GWAS) are designed to discover genetic variants such as single nucleotide polymorphisms (SNPs) that are associated with human complex traits. Although there is an increasing interest in the application of GWAS methodologies to population-based cohorts, many published GWAS have adopted a case-control design, which raise an issue related to a sampling bias of both case and control samples. Because of unequal selection probabilities between cases and controls, the samples are not representative of the population that they are purported to represent. Therefore, non-random sampling in case-control study can potentially lead to inconsistent and biased estimates of SNP-trait associations. In this paper, we proposed inverse-probability of sampling weights based on disease prevalence to eliminate a case-control sampling bias in estimation and testing for association between SNPs and quantitative traits. We apply the proposed method to a data from the Korea Association Resource project and show that the standard estimators applied to the weighted data yield unbiased estimates.

• Development and Reproduction
• /
• 제4권2호
• /
• pp.153-160
• /
• 2000

In this study, genetic variation in the 5 strains of 2N-cont, meiotic-G2N, mitotic-G2N and two types of clones with different genetic backgrounds was investigated by developing their tolerance to water temperature and salinity, which is a physiological trait, into a quantitative trait. The temperature was set at 19$^{\circ}C$, 22.5$^{\circ}C$, 25$^{\circ}C$ and 30$^{\circ}C$, each of which was combined with 0$\textperthousand$, 15$\textperthousand$ and 30$\textperthousand$ of salinity respectively, making 12 groups in all. In the mean survival time (MST), samples with 15$\textperthousand$ of salinity showed the longest survival time at all temperatures. The 2N-cont had the longest 126.16 h followed by clone-11 and clone-15 surviving for 113.22 h and 91.05 h respectively. Gynogenetic diploids showed the shortest 87,32 h and 36.56 h. At 22.5 and 25$^{\circ}C$, MST of each strain was significantly short, showing similar results to those of the groups at 19$^{\circ}C$. The 2N-cont had the longest MST while clones had a longer MST than gynogenetic diploids. This could be due to gynogenesis which causes homozygosis among malignant harmful genes, leading to its appearance in populations and resulting in early death in individuals with such genes. On the other hand, MST of clones was longer than that of gynogenetic fish. This could be because the 1st gynogenetic generation, which is a parental population, has already had its malignant genes removed, while the clones of the 2nd gynogenetic generation have had their superior genes fixed as well as their tolerance and survival improved. When temperature was raised to 22.5$^{\circ}C$ and 25$^{\circ}C$, increase in variation was observed in gynogenetic diploids and decrease in clones in 15$\textperthousand$ of salinity. This shows that such a trait is genetic to a certain extent. Consequently, if this character is developed into a quantitative trait and applied to selective breeding, it could be a useful character to secure superior strains and individuals, and also it would be possible to improve populations genetically through selection.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권9호
• /
• pp.1210-1213
• /
• 2004

Quantitative trait loci (QTL) mapping can be applied to detect chromosomal locations that control economic traits in farm animals. Teat number has been considered as one of the most important factors to evaluate mothering ability of sow. Especially, teat number is more important when the number is less than the litter size. This study was conducted to identify QTL affecting teat number in the Korean native pig${\times}$Landrace resource family. A total of 240 animals was genotyped for 132 polymorphic microsatellites covering the 18 pig autosomes. Mean and standard deviation of teat number in $F_2$animals is 13.46${\pm}$1.40. QTL was analyzed using F2 QTL Analysis Servlet of QTL express. A QTL for teat number on SSC9 was significant at the 1% chromosome-wide level and three suggestive QTL were detected on SSC3, 7 and 14. All QTL detected in this study had additive effect and Landrace alleles were associated with higher teat number in comparison with Korean native pig for three of four QTL.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권10호
• /
• pp.1350-1353
• /
• 2004

To further investigate the regions on porcine chromosome 7 that are responsible for economically important traits, phenotypic data from a total of 287 F2 individuals were collected and analyzed from 1998 to 2000. All animals were genotyped for eight microsatellite loci spanning the length of chromosome 7. QTL analysis was performed using interval mapping under the line-cross model. A permutation test was used to establish significance levels associated with QTL effects. Observed QTL effects were (chromosomewide significance, position of maximum significance in centimorgans): Birth weight (<0.01, 3); Carcass length (<0.05, 80); Longissimus muscle area (<0.01, 69); Skin percentage (<0.01, 69); Bone percentage (<0.01, 74); Fat depths at shoulder (<0.05, 54);Mean fat depth (<0.05, 81); Moisture in m. Longissimus Dorsi (<0.05, 88). Additional evidence was also found which suggested QTL for dressing percentage and fat depths at buttock. This study offers confirmation of several QTL affecting growth and carcass traits on SSC7 and provides an important step in the search for the actual major genes involved in the traits of economic interest.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권3호
• /
• pp.287-291
• /
• 2010

The purpose of this study was to detect quantitative trait loci (QTL) for growth and carcass quality traits on BTA6 in a population of Hanwoo cattle. Three hundred and sixty one steers were produced from 39 sires that were sired by 17 grandsires in the two Hanwoo farming branches of the National Livestock Research Institute of Korea, between Spring 2000 and Fall 2002. DNA samples were collected for all of the steers, sires and grandsires, and the phenotypes for six growth and carcass quality traits were measured at 24 months of age. Twelve microsatellite markers were chosen on BTA6 and a linkage map was constructed by using seven of the twelve markers. Then, a chromosome-wide QTL scan was performed by applying an Animal Model, in which effects of QTL alleles within the grand sires were fitted as a random term. Three QTL were detected at the 5% chromosome-wise level for backfat thickness, average daily gain, and final weight. The most likely positions for the QTL were in the proximal region, i.e. 0 cM, 35 cM, and 63 cM, respectively. Also, another QTL for longissimus dorsi muscle area was detected at the 10% chromosome-wise level at 67 cM. These results were, in general, consistent with our previous report, in which candidate gene analyses showed that a SNP near ILSTS035 flanked by BM4621 (62.5 cM) and BMS2460 (81.3 cM) was associated with final weight, carcass weight, average daily gain, and longissimus dorsi muscle area in the same Hanwoo population.

• Asian-Australasian Journal of Animal Sciences
• /
• 제30권8호
• /
• pp.1081-1085
• /
• 2017

Objective: Previously, we reported quantitative trait loci (QTLs) affecting backfat thickness (BFT) traits on pig chromosome 5 (SW1482-SW963) in an F2 intercross population between Landrace and Korean native pigs. The aim of this study was to evaluate glutamate receptor-interacting protein 1 (GRIP1) as a positional candidate gene underlying the QTL affecting BFT traits. Methods: Genotype and phenotype analyses were performed using the 1,105 $F_2$ progeny. A mixed-effect linear model was used to access association between these single nucleotide polymorphism (SNP) markers and the BFT traits in the $F_2$ intercross population. Results: Highly significant associations of two informative SNPs (c.2442 T>C, c.3316 C>G [R1106G]) in GRIP1 with BFT traits were detected. In addition, the two SNPs were used to construct haplotypes that were also highly associated with the BFT traits. Conclusion: The SNPs and haplotypes of the GRIP1 gene determined in this study can contribute to understand the genetic structure of BFT traits in pigs.