• Mass Spectrometry Letters
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• 제13권4호
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• pp.139-145
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• 2022

Protein glycosylation is a common post-translational modification by non-template-based biosynthesis. In fungal biotechnology, which has great applications in pharmaceuticals and industries, the importance of research on fungal glycoproteins and glycans is accelerating. In particular, the importance of quantitative analysis of fungal glycans is emerging in research on the production of filamentous fungal proteins by genetic modification. Reliable mass spectrometry-based techniques for quantitative glycomics have evolved into chemical, enzymatic, and metabolic stable isotope labeling methods. In this study, we intend to expand quantitative glycomics by metabolic isotope labeling of glycans in Aspergillus niger, a filamentous fungus model, by the MILPIG method. We demonstrate that incubation of filamentous fungi in a culture medium with carbon-13 labeled glucose (1-¹³C₁) efficiently incorporates carbon-13 into N-linked glycans. In addition, for quantitative validation of this method, light and heavy glycans are mixed 1:1 to show the performance of quantitative analysis of various N-linked glycans simultaneously. We have successfully quantified fungal glycans by MILPIG and expect it to be widely applicable to glycan expression levels under various biological conditions in fungi.

• 대한방사선기술학회지:방사선기술과학
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• 제41권4호
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• pp.329-337
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• 2018

This study was investigated quantitative ultrasound (QUS) usage in Korea for the QUS quality control guidelines. A total of 344 questionnaires collected from July 24th to August 25th 2017 were analyzed. Questionnaires were created through user interviews, expert group advice, literature review and field observation. As a result of the general characteristics of quantitative ultrasound holding amounted to 81.98% of clinic and 6.69% of hospitals. The main user was radio-logical technologists as 31.39%. The contact methods of the gel pad (balloon) were the most used at 56.68% and the scan region was 91.9% of calcaneus. The quantitative ultrasound quality control cycle was 67.37% when the abnormality was found in the equipment, and 63.66% when the accuracy control was implemented according to the manual. The phantoms of QUS were 34.30% of the manufacturer's own phantoms. User of QUS had never received education for quality control of quantitative ultrasound as 62.20%. This study was expected to be useful when creating detailed quality control guidelines in the future, as well as guidelines for the quality control of Korea's standard quantitative ultrasound.

• 웰빙융합연구
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• 제6권2호
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• pp.39-46
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• 2023

Purpose: The small-scale environmental impact assessment system in Korea was introduced and implemented in August 2000, but it has a problem that it cannot guarantee implementation due to the large proportion of qualitative reduction measures for each evaluation item. Therefore, when preparing a small-scale environmental impact assessment, research was conducted on how to improve the existing simple listing-type reduction measures and qualitative evaluation standards to quantitative reduction measures and evaluation standards reflecting regional characteristics. Research design, data and methodology: The small-scale environmental impact assessment system in Korea was introduced and implemented in August 2000, but it has a problem that it cannot guarantee implementation due to the large proportion of qualitative reduction measures for each evaluation item. Therefore, when preparing a small-scale environmental impact assessment, research was conducted on how to improve the existing simple listing-type reduction measures and qualitative evaluation standards to quantitative reduction measures and evaluation standards reflecting regional characteristics. Results: As a result of the analysis of qualitative and quantitative factors, the arithmetic sum of the qualitative factors of the total six projects is 160, accounting for 80% of the total number of reduction measures, and the quantitative factors are 40, accounting for 20%. Among them, the number of qualitative reduction measures reached 97.4% for animal and plant items, and more than 90% for air quality, noise and vibration, and eco-friendly resource circulation items. Conclusions: Therefore, it is necessary to avoid establishing qualitative reduction measures and set quantitative measures as the basis, but to specify the specifications, size, and installation location related to the reduction measures, and to calculate the numerical reduction efficiency.

• Food Science and Biotechnology
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• 제17권3호
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• pp.573-577
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• 2008

$^1H$-Nuclear magnetic resonance (NMR) spectrometry was applied to the quantitative analysis of coumarins in the roots of Angelica gigas without any chromatographic purification. The experiment was performed by the analysis of each singlet germinal methyl, which was well separated in the range of 1.0-2.0 ppm in the $^1H$-NMR spectrum. The quantity of the compounds was calculated by the ratio of the intensity of each compound to the known amount of internal standard (dimethyl terephthalate). These results were compared with the conventional gas chromatography (GC) method. The contents of decursin and decursinol angelate in A. gigas were determined $1.98{\pm}0.07$, $1.13{\pm}0.08%$ in quantitative $^1H$-NMR method and $2.06{\pm}0.24$, $1.17{\pm}0.24%$ in GC method, respectively. The advantages of quantitative $^1H$-NMR analysis are that can be analyzed to identify and quantify, and no reference compounds required for calibration curves. Besides, it allows rapid and simple quantification for coumarins with an analysis time for only 10 min without any preprocessing.

• 한국자기공명학회논문지
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• 제11권2호
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• pp.115-121
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• 2007

A new quantitative analysis of chloride by the HERETIC NMR method which does not need internal or external references was described. The results showed that the use of HERETIC peak corresponding $500\;{\mu}g/mL$ of chloride calibration showed less than 4 % standard deviations from 50 to $5000\;{\mu}g/mL$ range of chloride concentrations.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권2호
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• pp.149-153
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• 2002

Induction of non-diapause eggs and manifestation of quantitative characters were studied in different seasons by low temperature incubation of eggs of a low yielding diapause with coloured cocoons silkworm breed, RDI of Bombyx mori. Hundred percent of nandiapause eggs were induced when the egg incubation was carried out at 15$^{\circ}C$ followed by incubation initially at $25^{\circ}C$ and then at $15^{\circ}C$. The diapause-nondia-pause relation was found to be reversible. Analysis of variance study demonstrated significant variation in all the quantitative characters whereas significant variation due to different incubation treatments was observed for larval span, fifth instar larval span, cocoon yield/10,000 larvae by weight, cocoon weight, cocoon shell weight, and cocoon shell ratio. study on hybrid vigour skewed significant heterosis over mid parent value for four economic characters viz., cocoon weight, cocoon shell weight, cocoon shell ratio and filament length in a Fl hybrid between RD1 with diapause eggs and Japanese type bivoltine $NB_{4} D_{2}$.

• 한국국방경영분석학회지
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• 제22권2호
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• pp.166-181
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• 1996

Aircraft survivability is determined by the susceptibility and the vulnerability. The aircraft susceptibility and vulnerability depend upon the hardware and software factors. Each of the hardware and software factors consisted of the qualitative and quantitative attributes varies according to the time of the mission. In order to establish the mathermatical model to analyze and evaluate the aircraft survivability, qualitative factors have to be transformed into quantitative factors. Even if many researches in the area of dynamic concept analysis and conversion of qualitative factors into the quantitative factors has been insufficient. This research enhances these insufficient area by developing a reliable aircarft survivability analysis method. The major areas of this research are as follows. First, a method for the conversion of the qualitative factors into the quantitative factors is developed by combining the Fuzzy Set Theory concept and the Delphi Technique. Second, by using the stochastic network diagram for the dynamic survivability analysis, the aircraft survivability and the probability of kill are calculated from the state probability for the situation during mission. The advantage of the analysis technique developed in this research includes ease of use and flexibility. In other words, in any given aircraft's mission execution under any variable probability density function, the developed computer program is able to analyze and evaluate the aircraft survivability.

• 한국도서관정보학회지
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• 제24권
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• pp.107-161
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• 1996

This study aims at introducing 'ethnography' which is one of the most representative qualitative research methods into Library and Information Science research in Korea.. Ethnography, derived from anthropology, helps researchers to understand the whole and deep aspect of the research object. That is because the researcher puts himself into the life-world of the research object and observes it for a long time. Ethnography can be used as an alternative method to quantitative research methods. Until now, Library and Information Science research in Korea has used quantitative research methods in almost every case. From the 1980s so-called 'scientific methods' using hypotheses testing, have provided the major premise for research methodology in Library and Information Science of Korea. And the researchers have seen their research object(Korean Libraries) not in the native perspective but largely in the western(especially American) perspective. There is a need in Korea for more culturally relative research. So the desirability of introducing ethnography and other qualitative research methods into Library and Information Science research in Korea can be summarized as follows : I. Ethnography and other qualitative methods are needed for the researchers to overcome the limitation of quantitative methods which have formed the main methodological paradigm in Library and Information Science research in Korea. While those quantitative scientific methods can be a n.0, pplied to the social sciences, they are not adequate for the social sciences. It is because the research objects of the social sciences are human and social phenomena. II. It is needed that Library and Information Science research in Korea pay more attention to the speciality of Korean libraries. To do researches based on the viewpoint of cultural-relativism, researchers should consider the cultural context of Korean libraries. During the past years researchers in other social science fields in Korea, especially sociology and pedagogy, have gradually a n.0, pplied the methods of ethnography to their fields. These social scientists have attempted to escape from ethnocentrism, a problem which has greatly influenced past and present research methods. To get a holistic and in-depth understanding of Korean libraries on the present stage, and to solve their problems radically, it seems imperative that Library and Information Science research in Korea pay more attention to qualitative research methods such as ethnography.

• Korean Journal of Radiology
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• 제25권6호
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• pp.518-539
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• 2024

Coronary computed tomography angiography (CCTA) has emerged as a pivotal tool for diagnosing and risk-stratifying patients with suspected coronary artery disease (CAD). Recent advancements in image analysis and artificial intelligence (AI) techniques have enabled the comprehensive quantitative analysis of coronary atherosclerosis. Fully quantitative assessments of coronary stenosis and lumen attenuation have improved the accuracy of assessing stenosis severity and predicting hemodynamically significant lesions. In addition to stenosis evaluation, quantitative plaque analysis plays a crucial role in predicting and monitoring CAD progression. Studies have demonstrated that the quantitative assessment of plaque subtypes based on CT attenuation provides a nuanced understanding of plaque characteristics and their association with cardiovascular events. Quantitative analysis of serial CCTA scans offers a unique perspective on the impact of medical therapies on plaque modification. However, challenges such as time-intensive analyses and variability in software platforms still need to be addressed for broader clinical implementation. The paradigm of CCTA has shifted towards comprehensive quantitative plaque analysis facilitated by technological advancements. As these methods continue to evolve, their integration into routine clinical practice has the potential to enhance risk assessment and guide individualized patient management. This article reviews the evolving landscape of quantitative plaque analysis in CCTA and explores its applications and limitations.

• ETRI Journal
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• 제28권5호
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• pp.621-630
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• 2006

Observation of the effects of a handset subsidy on the mobile telecommunication industry has revealed two different aspects. The activation of various mobile services and the handset market, has led to the rapid acceleration of the related technological development, which is a desirable result, while rising prices and the overspending of related resources are undesirable. A great deal of research has been conducted to assess both desirable and undesirable factors using qualitative methods. However, quantitative studies into the effects of a handset subsidy are rare. In this study, we consider the positive and negative effects on consumer welfare of a change in demand and prices brought about by a handset subsidy. Then, we quantitatively compare the positive and negative effects and analyze their direct effects on consumer welfare.