• Asian Pacific Journal of Cancer Prevention
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• 제17권1호
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• pp.219-223
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• 2016

Background: Promoter hypermethylation is a frequent epigenetic mechanism for gene transcription repression in cancer and is one of the hallmarks of the disease. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) contributes to cell contact-mediated communication. Dysregulation of promoter methylation has been reported in various cancers. Objectives: The objectives of this study were to investigate the CELSR3 hypermethylation level in oral squamous cell carcinomas (OSCCs) using methylation-sensitive high-resolution melting analysis (MS-HRM) and to correlate CELSR3 methylation with patient demographic and clinicopathological parameters. Materials and Methods: Frozen tissue samples of healthy subjects' normal mucosa and OSCCs were examined with regard to their methylation levels of the CELSR3 gene using MS-HRM. Results: MS-HRM analysis revealed a high methylation level of CELSR3 in 86% of OSCC cases. Significant correlations were found between CELSR3 quantitative methylation levels with patient ethnicity (P=0.005), age (P=0.024) and pathological stages (P=0.004). A moderate positive correlation between CELSR3 and patient age was also evident (R=0.444, P=0.001). Conclusions: CELSR3 promoter hypermethylation may be an important mechanism involved in oral carcinogenesis. It may thus be used as a biomarker in OSCC prognostication.

• 대한핵의학회지
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• 제19권2호
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• pp.43-50
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• 1985

With the improvement of hemodialysis, the course of the disease in patient with endstage renal disease has been clearly improved. Nevertheless, among several shortcomings to our present mode of renal replacement therapy, cardiovascular complications have been the leading cause of morbidity and mortality. Several factors such as anemia, arteriovenous shunting of blood, intermittent extracorporeal circulation and hypertension may be contributing. But little is known about the quantitative cardiac hemodynamic characteristics occurred during hemodialysis. The purpose of this study is to observe the sequential hemodynamic changes before, during and after the hemodialysis and to investigate reliable parameters in the detection of ventricular dysfunction. In the present study, equilibrium radionuclide cardiac angiography was performed and left and right ventricular volume indices, ejection phase indices of both ventricular, performance were measured in the 16 stable patients with chronic renal failure treated with maintenance hemodialysis sequentially i.e. before, during (early and late phase) and after the hemodialysis. The results obtained were as follows; 1) The indices of the left ventricular function were not changed during the hemodialysis but increased after the hemodialysis. 2) The indices of the right ventricular function(EF, SVI) were significantly decreased in the early phase (15, 30 minutes after starting extracorporeal circulation) but recovered after the hemodialysis. 3) The ratio of right ventricular to left ventricular ejection fraction was significantly decreased in the early phase and the lung volume indices were significantly increased at the same phase. As a conclusion, hemodialysis improves left ventricular function maybe due to increased contractility, and effects on the right ventricular function maybe due to the increased lung volume in the early phase of hemodialysis.

• Asian Pacific Journal of Cancer Prevention
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• 제16권4호
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• pp.1397-1401
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• 2015

Background: To determine the expressions of Tbx3, a member of subgroup belonging to T-box family, and its prognostic value in pancreatic carcinoma. Materials and Methods: We determined the expression levels of Tbx3 on both mRNA and protein levels in 30 pairs of fresh tumor tissues and paratumor tissues by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively. In addition, protein level of Tbx3 were identified using immunochemistry in 80 pairs of paraffin-embedded specimen. The correlations between Tbx3 expression and various clinicopathological parameters as well as overall survival were evaluated. Results: Tbx3 mRNA and protein levels in tumor tissues were significantly higher than in the paratumor tissues by qRT-PCR ($0.05{\pm}0.007$ vs. $0.087{\pm}0.001$, p<0.001) and western blotting ($1.134{\pm}0.043$ vs. $0.287{\pm}0.017$, p<0.001). The statistical analysis based on immunohistochemical evaluation suggested that Tbx3 aberrant expression was significantly associated with several conventional clinicopathological variables, such as gender, age, tumor position, preoperative CA19-9 level, pathological T staging and N staging. Univariate and multivariate analyses revealed that Tbx3 expression was an independent prognostic factor for overall survival (<0.001). Conclusions: Our results suggest that overexpression of Tbx3 is associated with poor prognosis of pancreatic cancer patients. However, additional clinical trials are needed to accurately validate this observation.

• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.2851-2857
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• 2013

Objective: REPS2 plays important roles in inhibiting cell proliferation, migration and in inducing apoptosis of cancer cells, now being identified as a useful biomarker for favorable prognosis in prostate and breast cancers. The purpose of this study was to assess REPS2 expression and to explore its role in esophageal squamous cell carcinoma (ESCC). Methods: Protein expression of REPS2 in ESCCs and adjacent non-cancerous tissues from 120 patients was analyzed by immunohistochemistry and correlated with clinicopathological parameters and patient outcome. Additionally, thirty paired ESCC tissues and four ESCC cell lines and one normal human esophageal epithelial cell line were evaluated for REPS2 mRNA and protein expression levels by quantitative RT-PCR and Western blotting. Results: REPS2 mRNA and protein expression levels were down-regulated in ESCC tissues and cell lines. Low protein levels were significantly associated with primary tumour, TNM stage, lymph node metastasis and recurrence (all, P < 0.05). Survival analysis demonstrated that decreased REPS2 expression was significantly associated with shorter overall survival and disease-free survival (both, P < 0.001), especially in early stage ESCC patients. When REPS2 expression and lymph node metastasis status were combined, patients with low REPS2 expression/lymph node (+) had both poorer overall and disease-free survival than others (both, P < 0.001). Cox multivariate regression analysis further revealed REPS2 to be an independent prognostic factor for ESCC patients. Conclusions: Our findings demonstrate that downregulation of REPS2 may contribute to malignant progression of ESCC and represent a novel prognostic marker and a potential therapeutic target for ESCC patients.

• Journal of Korean Neurosurgical Society
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• 제54권4호
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• pp.280-288
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• 2013

Objective : Perfusion computed tomography (PCT) has the ability to measure quantitative value and produce maps of mean transit time (MTT), cerebral blood flow (CBF), and cerebral blood volume (CBV). We assessed cerebral hemodynamics by using these parameters and acetazolamide (ACZ) challenge for pre- and post-procedural evaluation in patients with unilateral cerebrovascular stenotic disease. Methods : Thirty patients underwent pre-procedural PCT with ACZ challenge, and 24 patients (80%) was conducted follow up PCT after angioplasty with same protocol. The mean MTT, CBF, and CBV were measured and compared in both middle cerebral arterial (MCA) territories before and after ACZ challenge. Hemispheric ratio and percent change after ACZ challenge were calculated before and after angioplasty. Results : The mean stenosis rate was 76.6%. Significant increases in MTT (32.6%, p=0.000) and significant decreases in CBF (-14.2%, p=0.000) were found in stenotic side MCA territories. After ACZ challenge, there were significant changes in MTT (37.4%, p=0.000), CBF (-13.1%, p=0.000), and CBV (-10.5%, p=0.001) in pre-procedural perfusion study. However, no significant increases were found in MTT, or decreases in CBF and CBV in post-procedural study. There were no significant changes after ACZ challenge also. In addition, the degrees of these changes (before and after ACZ challenge) were highly correlated with the stenotic degrees in pre-procedural perfusion study. Conclusion : PCT with ACZ challenge appears to be a useful tool to assess the cerebral perfusion status especially in patients with unilateral symptomatic stenotic disease.

• 정보처리학회논문지C
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• 제12C권6호
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• pp.855-864
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• 2005

본 논문에서는 악성 코드, 인터넷 웜과 같은 비정상 트래픽의 생성을 탐지하고 대응하는 침입탐지시스템 구조를 제안한다. 제안된 시스템의 경보정보 대응능력 성능분석을 위하여 시스템 모델링을 수행하고, OPNET을 이용하여 시뮬레이터를 설계하고 구현한다. 먼저 비정상적인 트래픽으로 부터 초래되는 경보정보의 도착 프로세스를 모델링 한다. 경보정보가 집중적으로 발생하는 상황을 모델링하기 위하여 트래픽의 burstiness(군집성)를 잘 나타낼 수 있는 IBP(Interrupted Bunoulli Process)를 적용한다. 다음에 성능파라미터에 대한 시스템의 정량적인 이해를 위하여 모의실험을 수행한다. 성능분석 결과를 바탕으로 보안노드의 고속화를 저해하는 요인을 분석하고 성능을 향상시키기 위한 방안을 도출 하고자 한다.

• KSBB Journal
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• 제18권5호
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• pp.347-351
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• 2003

초임계 이산화탄소를 이용하여 감초의 glycyrrhizic acid를 추출하기 위하여 일정 유속 (3 mL/min)에서 추출온도와 압력 보조용매의 종류와 첨가량에 대한 효과를 조사하여 보았다. 순수한 초임계 이산화탄소만을 사용해서 감초로부터 glycyrrhizic acid를 거의 추출할 수 없었다. 99.9% methanol, ethanol과 isopropanol을 보조용매로 초임계 이산화탄소에 첨가하여 추출한 경우, 추출수율의 증대에 큰 영향을 미치지 않았으나, 70% (v/v) aqueous methanol, ethanol과 isopropanol을 이산화탄소에 10% (v/v)으로 첨가한 경우에는 급격한 수율의 증대를 볼 수 있었고, 70% (v/v) aqueous methanol을 첨가하였을 때가 96.62 wt%로 가장 높은 회수율을 나타내었다. 한편, 70% (v/v) aqueous ethanol을 보조용매로 사용한 경우의 가장 높은 회수율과 빠른 추출속도를 나타낸 추출압력과 추출온도는 각각 40 MPa과 8$0^{\circ}C$였고, 이 조건하에서 다양한 농도의 aqueous ethanol (50∼90% (v/v))을 보조용매로 사용하였을 때 60% (v/v) aqueous ethanol을 보조용매로 첨가한 경우가 104.11 wt%로 가장 높은 회수율과 빠른 추출속도를 나타내었다. 또한 이산화탄소에 대한 60% (v/v) aqueous ethanol의 첨가량은 15% (v/v)을 첨가하였을 때 60분 내에 최고 회수율 (104.57 wt%)에 도달하였다.

• 한국산학기술학회논문지
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• 제20권12호
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• pp.280-286
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• 2019

본 연구는 발의 피로와 통증을 줄이고 발의 정상적인 기능을 유지해주는 기능적 인솔 적용 유무가 노인 보행 시 하지의 생체 역학적 변인 및 패턴에 미치는 영향을 규명하고자 하였다. 본 연구의 대상자는 10명(연령, 73.2 세: 신장, 152.1 cm: 체중, 59.4 kg)이며, 기능적 인솔 착용과 비 착용 시의 보행을 분석하였다. 보행분석은 퀄리시스 시스템 (Qualisys system)으로 촬영 후 비쥬얼 3D (Visual-3D)로 분석하였다. 그 결과, 기능적 인솔을 착용했을 경우, 보장과 유각기 속도가 유의미하게 증가하였다 (p<.05). 또한 하지의 신전 모멘트가 입각기에서 유의미하게 증가하였다 (p<.05). 본 연구 결과 기능적 인솔은 노인의 보행 안정성을 향상시는 효과가 있었다. 그러나 이 연구의 결과를 일반화하기 위해서는 더 많은 대상자들을 통해 보다 많은 정량적 자료를 축적할 필요가 있으며, 대상자들에게 오랜 시간동안 인솔을 사용하게 한 후 보행변인 및 패턴의 변화를 검토하는 연구가 추후 이뤄져야 할 것으로 판단된다.

• 융합신호처리학회논문지
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• 제9권2호
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• pp.112-120
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• 2008

본 연구는 전통적인 수지(手指)를 이용한 진맥환경 하에서 객관성 있는 정량적 정보를 수집하기 위해 한의학 진단법에 입각한 맥상(脈象)과 병상(病象)진단을 위한 시스템을 개발하는데 그 목적이 있다. 이를 위하여 우선, 최적합한 진단정보를 취득하기 위한 진맥기로서 골무형태의 센서유닛을 제안하고, 이것을 인지, 중지, 약지에 장착하여 전통적인 방법으로 척관촌(尺關寸)점의 맥압파를 측정하는 방법을 제시하였다. 다음으로, 이 진맥기를 사용하여 한의 진단학에서 정의하고 있는 부침지삭허실(浮沈遲數虛實)맥으로 표현되는 육조맥(六祖脈)과 홍(洪), 유(濡), 혁(革), 산(散)맥 등을 포함하는 28맥을 객관적으로 인식할 수 있음을 해석적으로 밝혔다. 끝으로, 이 진맥기로서 측정한 압맥파로부터 맥상 진단에 기초가 되는 주요 파라미터를 추출하는 방법을 제시했다.

• 한국수산과학회지
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• 제39권spc1호
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• pp.214-222
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• 2006

Dominant species of macrobenthos were analyzed based on differentiation of three distinct methods: the density based method, the biomass based method and LeBris method, by considering the frequency of occurrence using quantitative data collected over 5 years (1993-1998) at 21 stations in Chonsu Bay. Sedimentary environments as well as species composition and diversity showed the spatial and temporal distribution patterns. The ranks of dominant species as determined by the density based method were more similar to the results by the LeBris method than to those from the biomass based method. Considering the temporal variation, LeBris method were more efficient than any other methods for the determination of dominant species in Chonsu Bay. Lumbrineris longifolia, Theora fragilis, and Moerella jedoensis were recognized by all three methods. A one-way analysis of variance indicated spatial distributions patterns among most of the dominant species. These species showed positive correlations to sedimentary parameters such as mean grain size. However, T. fragilis and Paraprinospio pinnata showed the temporal patterns in their distribution, and were also correlated to the benthic environment, organic content and dissolved oxygen. Some dominant species, e.g., T. fragilis, S. scutata, G. gurjanovae proved to be useful benthic indicators based on the environmental variations determinated by long-term benthic ecological monitoring in Chonsu Bay.