• Title/Summary/Keyword: Quantitative Research Method

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Quantitative Analysis of Two Genetically Modified Maize Lines by Real-Time PCR

  • Lee Seong-Hun;Kang Sang-Ho;Park Yong-Hwan;Min Dong-Myung;Kim Young-Mi
    • Journal of Microbiology and Biotechnology
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    • v.16 no.2
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    • pp.205-211
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    • 2006
  • A quantitative analytical method to detect new lines of genetically modified (GM) maize, NK603 and TC1507, has been developed by using a real-time polymerase chain reaction (PCR). To detect these GM lines, two specific primer pairs and probes were designed. A plasmid as a reference molecule was constructed from an endogenous DNA sequence of maize, a universal sequence of a cauliflower mosaic virus (CaMV) 35S promoter used in most GMOs, and each DNA sequence specific to the NK603 and TC1507 lines. For the validation of this method, the test samples of 0, 0.1, 0.5, 1.0, 3.0, 5.0, and 10.0% each of the NK603 and TC1507 GM maize were quantitated. At the 3.0% level, the biases (mean vs. true value) for the NK603 and TC1507 lines were 3.3% and 15.7%, respectively, and their relative standard deviations were 7.2% and 5.5%, respectively. These results indicate that the PCR method developed in this study can be used to quantitatively detect the NK603 and TC1507 lines of GM maize.

Age Related Analysis of Ultrasound Images of Normal Skeletal Muscle (연령에 따른 정상 골격근의 초음파 영상 분석)

  • Jeong, Jin-Gyu;Kim, Kye-Yoep;Kim, Jong-Man;Ki, Tae-Youl
    • Physical Therapy Korea
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    • v.13 no.1
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    • pp.47-53
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    • 2006
  • Since ultrasound has different reflections depending on components of organization, analysis of ultrasound images of skeletal muscle can offer both quantitative and qualitative reports as concerns skeletal muscle structure. This study is focused on the ultrasound method for evaluating the structural characteristics of skeletal muscle and also conducted to examine its practicality. After obtaining images of the elbow flexors from an ultrasound image device with 88 normal subjects whose ages were between twenty and seventy years old (44 men and 44 women), muscular density and white area index (WAI) which indicated structural characteristics of skeletal muscle were analyzed with gray scale analysis. The study examined correlations between subject's age and items which obtained from measuring ultrasound images and the differences in relations to sex and age. Muscular density and WAI had a high correlation with age and were significantly increased in men and women with greater age. The quantitative evaluation method of skeletal muscle structure which analyzed the ultrasound images has high practicality because it is a non-invasive method which complements physical therapy diagnosis and research methods and promotes functionality evaluation.

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Internal Factors Affecting Firm Performance: A Case Study in Vietnam

  • NGUYEN, Van Hau;NGUYEN, Thi Thu Cuc;NGUYEN, Van Thu;DO, Duc Tai
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.5
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    • pp.303-314
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    • 2021
  • The higher the firm performance, the more chances enterprises can expand and develop their production, create jobs, and improve the workers' living quality. The main objective of this study was to measure the internal factors influencing the firm's performance of food and beverage (F&B) firms listed on the Hanoi Stock Exchange (HNX). Data was collected on 15 F&B firms listed on the HNX from 2015 to 2019 We use mixed research method, both qualitative and quantitative. For the quantitative research method, the supporting tool is Stata13 software. The results via Ordinary Least Squares (OLS) regression method show the impacts of internal factors with the following observed variables: the ratio of short-term debt to total liabilities (CS1) and total assets (S2) have an opposite impact (-) on ROA and ROE; debt-to-total assets ratio (CS2) has an opposite effect (-) on ROA; growth of total assets (G2) of the growth factor positively affects (+) ROA and ROE, the remaining factors do not affect ROA and ROE; and internal factors do not influence ROS. Based on the findings, some recommendations have been proposed to help the F&B firms listed on the Hanoi Stock Exchange improving their firm performance in the future.

Discretization Method for Continuous Data using Wasserstein Distance (Wasserstein 거리를 이용한 연속형 변수 이산화 기법)

  • Ha, Sang-won;Kim, Han-joon
    • Database Research
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    • v.34 no.3
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    • pp.159-169
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    • 2018
  • Discretization of continuous variables intended to improve the performance of various algorithms such as data mining by transforming quantitative variables into qualitative variables. If we use appropriate discretization techniques for data, we can expect not only better performance of classification algorithms, but also accurate and concise interpretation of results and speed improvements. Various discretization techniques have been studied up to now, and however there is still demand of research on discretization studies. In this paper, we propose a new discretization technique to set the cut-point using Wasserstein distance with considering the distribution of continuous variable values with classes of data. We show the superiority of the proposed method through the performance comparison between the proposed method and the existing proven methods.

Change of MS Method and Comparison of SIFT-MS Method

  • CHOI, Jong-Sun;KWON, Lee-Seung;LEE, Ji-Hoon;KIM, Romertta;KWON, Woo-Taeg
    • Journal of Wellbeing Management and Applied Psychology
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    • v.5 no.1
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    • pp.37-46
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    • 2022
  • Purpose: This study examines the history of the evolution of MS analysis and intends to consider the future direction of technological development through the difference from the latest technology, SIFT-MS. Research design, data and methodology: A method of analysis will be described in detail at the below by SIFT-MS (Selected Ion Flow Mass Spectrometry), which is a technology developed by a company called SIFT Technologies. Results: The initial concept of mass spectrometry was begun in the late 1890s, and it continues to evolve even after the 21st century through the ripening stage of the 20th century. The development process of mass spectrometry by year has been described in detail in the Main text. Conclusions: Mass spectrometry, qualitative and quantitative analysis of substances plays a very important role in the research and medical fields. The development of these analytical methods is expected to continue in the future, and faster and more accurate qualitative analysis and mass spectrometry will be developed than the level currently reached. In addition, it is expected that hardware and software will be configured so that non-analysis experts can handle it easily, and it will be used as a technology that is more closely related to our lives.

A study on the quantitation of asbestos by the visual estimation and point counting method (시야평가법과 포인트계수법에 의한 석면정량평가 연구)

  • Choi, Yun-Ho;Kim, Tae-Hwa;Bae, Yong-Soo;Kim, Tae-Hyun;Kim, Hyeon-Ja;Jang, Eun-Ah;Hwang, Beom-Goo
    • Analytical Science and Technology
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    • v.27 no.3
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    • pp.153-160
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    • 2014
  • While variety of cases of studies about asbestos analysis methods are released internationally, the results of Asbestos Containing Materials (ACM) according to differences in the method of the analysis is becoming an issue. In this study, homogeneity ensured ACM samples were analyzed by visual estimation method and point counting method, and the result cound be used not only to improve the reliability on asbestos analysis of the institutions and analysts but also to obtain the basic data of Polarizing Light Microscope (PLM) analysis by comparing and evaluating. Asbestos analysis were divided into qualitative and quantitative analysis method. The quantitative analysis was performed by visual estimation method and point counting method (total 400 points) of EPA 600-R-93-116 method by using PLM. Firstly, The following was the result of homogeneity of the samples by ANOVA (Analysis of variance) and the results were satisfied. The results of qualitative analysis showed that the samples were chrysotile and amosite, and about the results of quantitative analysis, asbestos concentration determined by point counting method tend to be lower than concentrations determined by visual estimation method and also, pont counting method was a little more complicated and time-consuming.

Research Trends in Elementary Mathematics Education: Focused on the Papers Published in Domestic Journals During the Recent 5 Years (초등수학교육 연구동향 - 최근 5년간 게재된 국내 학술지 논문을 중심으로 -)

  • Ha, Su-Hyun;Pang, Jeong-Suk;Ju, Mi-Kyung
    • The Mathematical Education
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    • v.49 no.1
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    • pp.67-83
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    • 2010
  • The purpose of this study was to analyze the research trends of elementary mathematics education with regard to the topics, methods, subjects, and mathematical contents of such research. For this purpose, the papers published in domestic journals during the recent 5 years (2004-2009) were analyzed. A total of 383 papers from 8 professional journals were analyzed with the 4 criteria. First, the frequent research topics included instructional design and methods, learners' perspectives and abilities, and analysis of curriculum and textbooks in order. Second, qualitative research methodology was used twice as many as quantitative one. Whereas a survey was the most frequent quantitative research method, content analysis and case study were for qualitative methods. Third, research subjects included mainly typical students, specifically fifth and sixth graders. Papers dealing with lower graders or low-achievers as well as pre-service teachers were rare. Lastly, whereas the research on geometry as well as number and operations was active, that of measurement as well as probability and statistics was not. On the basis of these results, this paper includes several implications for the future research direction in elementary mathematics education.

A Quantitative Evaluation on Steel Corrosion by Polarization Resistance Method (분극저항법에 의한 철근부식의 정량적 평가)

  • 정우용;손영무;윤영수;변근주
    • Proceedings of the Korea Concrete Institute Conference
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    • 2000.04a
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    • pp.688-693
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    • 2000
  • Recently great efforts and investment have been made in order to evaluate concrete durability by steel corrosion. But most of study is relatively or qualitatively estimated, therefore it has a great limitation in evaluating a remaining service life of concrete. In this research, steel corrosion rate was measured quantitatively by polarization resistance method and multi-regressed considering chloride, carbonation, coverage depth, relative humidity, W/C, and the use of deicing salts. And a half cell potential method was used at th same time for the purpose of comparing with polarization resistance method.

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Studies on the Enzymatic Hydrolysis of Lignocellulosic Materials for the Alternative Fuels(III) - Quantitative Recycling of Cellulase Enzyme in the Enzymatic Hydrolysis of Steam-Exploded Woods - (대체연료(代替燃料) 생산(生産)을 위한 목질재료(木質材料)의 가수분해(加水分解)에 관한 연구(硏究) (III) - 폭쇄(爆碎)처리재의 산소분해시(酸素分解時) Cellulase 산소(酸素)의 정량적(定量的) 회수(回收)에 관하여 -)

  • Cho, Nam-Seok;Lim, Chang-Suk;Lee, Jae-Sung;Park, Shin
    • Journal of the Korean Wood Science and Technology
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    • v.19 no.1
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    • pp.14-21
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    • 1991
  • Steam-exploded woods were delignified by two-stage with a 0.3% NaOH extraction and oxygen-alkali bleaching and were subjected to the enzymatic hydrolysis with cellulase enzyme. Also, an improved almost quantitative recycle process of cellulase enzyme was discussed. In enzyme recovery by affinity method, The first recycling showed relatively high hydrolysis rate of 96.4%. Even at the third recycle, hydrolysis rate was 87.0 percents. In the case of cellulase recovery by ultrafiltration method, first 2 recycling treatments resulted in very high hydrolysis rates, 96.8% and 95.0%, respectively. Even the third recycling showed about 93.6%. Steam-explosion treatment of oak wood followed by 2-stage delignification with alkali and oxygen-alkali produced a excellant substrate for the enzymatic hydrolysis that allowed almost quantitative recycle of cellulase.

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The Recommended Approaches for the Evaluation of Testicular Toxicity with Awareness of the Spermatogenic Cycle and Quantitative Testicular Toxicity Evaluation Methods (정자생성 주기법을 이용한 고환독성 평가 필요성과 정량적인 고환독성 평가방법에 대한 고찰)

  • 손우찬;김종춘;유일재
    • Toxicological Research
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    • v.19 no.2
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    • pp.83-90
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    • 2003
  • Since histopathological examination was known to be the most sensitive evaluation for testicular toxicity, regulatory authorities have been published the guidelines on practical testicular assay approach. Those guidelines specified details of evaluation including fixation, embedding, stain-ing, histological examination and also seminiferous tubular staging methods. However, there have been confusing understanding among toxicologists and even pathologists on staging theory and its application on industrial testicular toxicity. Guidelines did not intend to conduct quantitative assay with staging but recommended the use of knowledge of staging. To count each tubular stage with statistical analysis is known to be time consuming and labor burdening work but the significance of toxicity has little value. It also has been pointed out that the application of staging theory for longer-term toxicity considered to be lacking of rationale. It could be recommended that qualitative assay with aware-ness of germ cell loss is more efficient method rather than quantitative counting of each tubular stage. Therefore it would be required that comprehensive understanding of testicular toxicity evaluation and the use of testicular staging method.