• Journal of Korean society of Dental Hygiene
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• v.19 no.3
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• pp.351-362
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• 2019

Objectives: The study aimed to analyze the factors affecting the maturity of dental biofilm, which was assessed with quantitative light-induced fluorescence-digital(QLF-D), in a sample of Korean older adults. Methods: This cross-sectional study included 67 participants, aged 65 years and older. All participants completed a questionnaire and tests to measure their manual dexterity and handgrip strength, which are parameters that indicate hand function abilities. To evaluate dental biofilm maturity, 804 surfaces of six index teeth were imaged using QLF-D and then quantified as ${\Delta}R$ values. All data were collected from May 25, 2017 to April 30, 2018. The independent t-test, one-way analysis of variance, and step-wise multiple linear regression were performed to analyze the factors associated with the maturity of dental biofilm (${\Delta}R$). Results: The multivariate linear regression analysis revealed that the factor most strongly related to dental biofilm maturity(${\Delta}R$) was manual dexterity (${\beta}=-0.326$), followed by handgrip strength (${\beta}=-0.303$) and use of interdental cleaning devices (${\beta}=-0.283$) (p<0.05). Conclusions: Manual dexterity, handgrip strength, and use of interdental cleaning devices are factors that can predict dental biofilm maturity in adults aged 65 years or older. Therefore, the hand function of a patient should be evaluated first, before assessing the oral hygiene status of the patient or providing him/her with oral health education, and the dental hygienist should provide differentiated oral hygiene care depending on the patient's hand function ability. Finally, dental hygienists should help older adults to recognize the importance of auxiliary oral hygiene devices such as interdental brushes and keep motivating them to use the devices more frequently.

• The Journal of the Korean dental association
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• v.55 no.2
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• pp.156-164
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• 2017

Purpose: The aim of this in vitro study was to assess changes in remineralization by stimulated human saliva over a short period of 48 hours with quantitative light-induced fluorescence (QLF) technology. Materials and Methods: Bovine incisor surfaces were demineralized for 10 days. Two types of stimulated saliva were collected from 7 healthy persons. 24 hours after tooth brushing (Stimulated saliva group) and immediately after tooth brushing with 1,000 ppm NaF dentifrice (Dentifrice saliva group). The specimens were immersed in saliva and fluorescence images were obtained by QLF-digital (QLF-D $biluminator^{TM}$,) at 2, 4, 6, 12, 24, and 48 hours fluorescence loss (${\Delta}F%$) of the lesions. A paired t-test was performed to assess fluorescence differences between before (${\Delta}F_{baseline}$) and after (${\Delta}F_{treatment\;time}$) the remineralization process. Results: Before the remineralization, the mean ${\Delta}F_{baseline}$ of the initial demineralized specimens was $-18.42{\pm}0.15$ (%). In both groups, the ${\Delta}F$ values obtained at baseline and after 2 hours were statistically significant (P < 0.001), indicating recovery of the lesions by approximately 40% after 2 hours. After 48 hours, remineralization rates were slightly higher (49%) for the stimulated saliva group than for the dentifrice saliva group (41%), but the difference was not statistically significant. Conclusions: With QLF minute degrees of remineralization by saliva can be measured in periods as short as 2 hours. Additionally no significantly higher effects of remineralization were observed in the dentifrice saliva group when compared to the stimulated saliva group.

• Journal of the korean academy of Pediatric Dentistry
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• v.37 no.1
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• pp.44-52
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• 2010

The objective of this in vitro study was to detect and monitor demineralization and remineralization of primary teeth according to restorative materials using quantitative light-induced fluorescence (QLF). A single bur hole was drilled on the each sound forty eight primary anterior teeth, and the specimens were divided into three groups. The cavity was restored with $Filtek^{TM}$ Z250(Group 1), F2000(Group 2), $Ketac^{TM}$ N100(Group 3) following the manufacturer's instructions. The teeth were subjected to the demineralizing buffer for 3 days, and then subjected to a remineralizing buffer for 14 days. The change of mineral loss(${\Delta}Q$) according to the stages was evaluated by QLF and the following results were obtained: 1. When demineralization was done, ${\Delta}Q$ was increased as follows. : Group 1 ($-110.79\;{\pm}\;27.77$) < Group 2 ($-104.84\;{\pm}\;28.95$) < Group 3 ($-90.16\;{\pm}\;21.87$) : Resistance to demineralization was statistically significant in Group 3. 2. There was a statistically significant increase in ${\Delta}Q$ of all groups since 1st day of remineralization 3. The rate of remineralization, ${\Delta}$(${\Delta}Q$)/day, showed significant high value in each group on the 1st day then decreased rapidly. 4. There was no statistically significant difference in the degree of remineralization among restorative materials.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.1
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• pp.739-744
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• 2020

This study evaluates the erosive potential and effects of mixed alcohols by analyzing the pH, titratable acidity, and fluorescence loss degree (△F). Following alcohol groups were investigated: Soju, Calamansi+soju, Yakult+soju, Cola+soju, and Energy drink+soju. The ratio of soju:beverage in the alcohol mixtures was 7:3. Ed. Notes: The sentence lacks clarity. Please review if the edit correctly portrays the meaning. If not, please revise appropriately. Measurement of the pH and titratable acidity (the amount of 1M NaoH solution required to raise to pH 5.5 (TA5.5) and 7.0 (TA7.0)) of alcohols was achieved by stirring with pH meter. The erosive effect of the alcohol mixtures on bovine tooth (△F) after 1, 2, 4, and 6 hours exposure were analyzed by quantitative light-induced fluorescence (QLF-D). All the mixed alcohols in this study showed an acidic pH, lower than 4.5. The average pH of mixed alcohols was 3.17 ± 0.50 whereas the pH of Soju was 8.6 ± 0.01. The TA5.5 and TA7.0 values of the mixed alcohols were 0.5~18 and 0.5~23.5, respectively. △F of the three tested mixed alcohol groups (except yakult+soju group) were observed to increase in a time-dependent manner. The calamansi mixed alcohol had the highest acidity potential and erosive effect among the tested groups. Taken together, the results indicate that the mixed alcohols have a strong erosive effect and potential on dental enamel.

• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.2
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• pp.287-296
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• 2008

The purpose of this in vitro study was to compare the remineralization effect of commercially available anticariogenic products, exactly low level fluoride mouthrinse(500 ppm NaF), tooth cream with Casein phosphopeptide-amorphous calcium phosphate(CPP-ACP) and fluoride mouthrinse plus tooth cream on artificial caries lesion. Artificial caries lesion was induced at the buccal surface of permanent third molar and the specimens were then divided(16 specimens/group) into four group. Twice a day during 28 days specimens of each group were treated with a commercially anticariogenic product as follows and applied to the pH cycling system. Group 1: control group (No treatment) Group 2: Tooth $Mousse^{(R)}$ (GC Co. Japan) Group 3: $chikachika^{(R)}$ (Samil Co. Korea) Group 4: $chikachika^{(R)}$+Tooth Mousse$^{(R)}$ The long-term change of mineral loss(${\Delta}Q$) was evaluated by quantitative light-induced fluorescence (QLF) and the following results were obtained: 1. ${\Delta}Q$ of Group 1 was not noticed statistically significant during 28 days comparing that prior to treatment. There was a statistically significant increase in ${\Delta}Q$ of Group 2 and 3 since 14 days. So was in ${\Delta}Q$ of Group 4 since 7 days. 2. ${\Delta}Q$ was increased as follows: Group 1< Group 2, 3< Group 4. 3. Comparing with Group 1, Group 2 was a statistically significant increase since 7 days and Group 3 and 4 were since 3 days. Comparing Group 2 with 3, there was not noticed statistically significant during whole duration. Group 4 was significantly higher than Group 2 and 3 after 28 days. 4. All groups demonstrated a decrease in the rate of remineralization as time goes on.

• The Journal of Korea Assosiation for Disability and Oral Health
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• v.13 no.2
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• pp.99-103
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• 2017

The primary responsibility for the oral hygiene of the disabled is usually the parents. Dental care of the disabled is early detection and recovery of lesions and continuous management. For this to be successful it is necessary to use diagnostic tool that can detect the early stages of dental caries which is difficult to detect with the naked eye. It is also important to educate and motivate the caregivers on oral hygiene management. Quantitative Light-induced Fluorescence-Digital (Billuminator, Inspektor Researh Systems BV, Amsterdam, The Netherlands), which provides overall caries inspection and visual information, can be useful for caregiver education. A 3-year-old girl who was hospitalized with Pallister-Killian syndrome, Hypothyroidism visited our clinic with chief complaint of rugged upper incisors. This girl had multiple dental caries and oral hygiene was very poor. Periodic QLF-D images were taken to provide caregiver education and oral hygiene management was improved. A 13-year-old girl with cerebral palsy visited our clinic for regular check up. Using QLF-D, we explained to the parents that there is a need for treatment of dental caries, and education of oral hygiene management was conducted. Improvement of oral hygiene in the disabled can be achieved through caregiver education. QLF-D is a diagnostic device that can detects early caries by irradiating light in the visible ray area to the teeth. It can also detects microleakage of restoration, plaque and calculus without disclosing agent. Clinicians can use the QLF-D to perform a general oral examination for the disabled. Also, QLF-D can be used to store visual information and educate caregivers. The accumulation of information using QLF-D makes it possible to provide feedback on oral care of parents, which is more advantageous for caregivers education.

• The korean journal of orthodontics
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• v.44 no.3
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• pp.113-118
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• 2014

Objective: The purpose of this in vitro study was to examine the effects of fluoridated, casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP)-containing, and functionalized ${\beta}$-tricalcium phosphate (fTCP)-containing toothpastes on remineralization of white spot lesions (WSLs) by using Quantitative light-induced fluorescence (QLF-D) Biluminator$^{TM}$ 2. Methods: Forty-eight premolars, extracted for orthodontic reasons from 12 patients, with artificially induced WSLs were randomly and equally assigned to four treatment groups: fluoride (1,000 ppm), CPP-ACP, fTCP (with sodium fluoride), and control (deionized water) groups. Specimens were treated twice daily for 2 weeks and stored in saliva solution (1:1 mixture of artificial and human stimulated saliva) otherwise. QLF-D Biluminator$^{TM}$ 2 was used to measure changes in fluorescence, indicating alterations in the mineral contents of the WSLs, immediately before and after the 2 weeks of treatment. Results: Fluorescence greatly increased in the fTCP and CPP-ACP groups compared with the fluoride and control groups, which did not show significant differences. Conclusions: fTCP- and CPP-ACP-containing toothpastes seem to be more effective in reducing WSLs than 1,000-ppm fluoride-containing toothpastes.

• Journal of the korean academy of Pediatric Dentistry
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• v.38 no.4
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• pp.317-326
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• 2011

QLF-D system composed with DSLR(digital single-lens reflex) camera, and the images of natural enamel caries and artificial caries was developed from 2 days to 14 days captured by QLF-D system. The correlation between lesion depth of the polarized microscope and luminosity ratio of QLF-D image was analyzed and the results were summarized as follows: 1. The Pearson correlation value between the lesion depth of polarized microscope images and luminosity ratio of QLF-D images was 0.969(p<0.01). 2. From Regression analysis of lesion depth from polarized image by demineralized period, the equation was y = 8.67x - 1.16(p<0.05). 3. From Regression analysis of luminosity ratio from QLF-D image by demineralized period, the equation was y = 3.53x + 6.42(p<0.05). From the results, QLF-D system can detect the enamel caries at the very early stage and can monitor the progression of demineralization and remineralization. For the convenient use of QLF-D system in the laboratory, the image analysing software was needed to analyze of interest site of enamel caries lesion.

• Journal of the korean academy of Pediatric Dentistry
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• v.43 no.4
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• pp.443-451
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• 2016

Subjects of this study were : $FluoroDose^{(R)}$ (FD, Centrix Inc., USA), $Enamelast^{TM}$ (EL, Ultradent Product Inc., USA), $Clinpro^{TM}$ white varnish (CW, 3M ESPE, USA), $CavityShield^{TM}$ (CS, 3M ESPE, USA), V $varnish^{TM}$ (VV, Vericom, Korea), MI $varnish^{TM}$ (MI, GC, Japan). The amount of fluoride ion release was measured eight times during 168 hours to see change in accumulation with the course of time using a measuring instrument. And the remineralization rate was measured with Quantitative Light-induced Fluorescence (QLF). V $varnish^{TM}$ group and MI $varnish^{TM}$ group showed high remineralization rates with statistically significance while $CavityShield^{TM}$ group was the lowest rate of remineralization (p < 0.05). After that, several chosen samples were scanned through electron microscope (SEM). Demineralized enamel was observed as the number of enamel crystal was very small; enamel rods and crystals were highly protruding. Remineralized groups with fluoride varnishes show the decreasing tendency of the surface roughness compared to the demineralized enamel.

• Journal of Korean Academy of Oral Health
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• v.41 no.1
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• pp.22-27
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• 2017

Objectives: Dental plaque is composed of 700 bacterial species. It is known that some oral microorganisms produce porphyrin, and thus, they emit red fluorescence when illuminated with blue light at a specific wavelength of <410 nm. Porphyromonas gingivalis belongs to the genus Porphyromonas, which is characterized by the production of porphyrin. The aim of this study was to evaluate red fluorescence emission of some oral microorganisms interacting with P. gingivalis. Methods: Five bacterial strains (P. gingivalis, Streptococcus mutans, Lactobacillus casei, Actinomyces naeslundii, and Fusobacterium nucleatum) were used for this study. Tryptic soy agar medium supplemented with hemin, vitamin K3, and sheep blood was used as a growth medium. The fluorescence emission of bacterial colonies was evaluated under 405 nm-wavelength blue light using a Quantitative Light-induced Fluorescence Digital (QLF-D) camera system. Each bacterium was cultured alone and co-cultured in close proximity with P. gingivalis. The red/green (R/G) ratio of fluorescence image was calculated and the differences of R/G ratio according to each growth condition were compared using the Mann-Whitney test (P<0.05). Results: Single cultured S. mutans, L. casei and A. naeslundii colonies emitted red fluorescence (R/G ratio=$2.15{\pm}0.06$, $4.31{\pm}0.17$, $5.52{\pm}1.29$, respectively). Fusobacterium nucleatum colonies emitted green fluorescence (R/G ratio=$1.36{\pm}0.06$). The R/G ratios of A. naeslundii and F. nucleatum were increased when P. gingivalis was co-cultured with each bacterium (P<0.05). In contrast, the R/G ratios of S. mutans and L. casei were decreased when P. gingivalis was co-cultured with each bacterium (P=0.002, 0.003). Conclusions: This study confirmed that P. gingivalis could affect the red fluorescence of other oral bacteria under 405 nm-wavelength blue light. Our findings concluded that P. gingivalis has an important role for red fluorescence emission of dental biofilm.