• Journal of Microbiology and Biotechnology
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• v.31 no.8
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• pp.1069-1078
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• 2021

Sevoflurane postconditioning (SPostC) has been proved effective in cardioprotection against myocardial ischemia/reperfusion injury. It was also reported that heat shock protein 70 (HSP70) could be induced by sevoflurane, which played a crucial role in hypoxic/reoxygenation (HR) injury of cardiomyocytes. However, the mechanism by which sevoflurane protects cardiomyocytes via HSP70 is still not understood. Here, we aimed to investigate the related mechanisms of SPostC inducing HSP70 expression to reduce the HR injury of cardiomyocytes. After the HR cardiomyocytes model was established, the cells transfected with siRNA for HSP70 (siHSP70) or not were treated with sevoflurane during reoxygenation. The lactate dehydrogenase (LDH) level was detected by colorimetry while cell viability and apoptosis were detected by MTT and flow cytometry. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting were used to detect HSP70, apoptosis-, cell cycle-associated factors, iNOS, and Cox-2 expressions. Enzyme-linked immuno sorbent assay (ELISA) was used to measure malondialdehyde (MDA) and superoxide dismutase (SOD). SPostC decreased apoptosis, cell injury, oxidative stress and inflammation and increased viability of HR-induced cardiomyocytes. In addition, SPostC downregulated Bax and cleaved caspase-3 levels, while SPostC upregulated Bcl-2, CDK-4, Cyclin D1, and HSP70 levels. SiHSP70 had the opposite effect that SPostC had on HR-induced cardiomyocytes. Moreover, siHSP70 further reversed the effect of SPostC on apoptosis, cell injury, oxidative stress, inflammation, viability and the expressions of HSP70, apoptosis-, and cell cycle-associated factors in HR-induced cardiomyocytes. In conclusion, this study demonstrates that SPostC can reduce the HR injury of cardiomyocytes by inducing HSP70 expression.

• Proceedings of the Korean Environmental Health Society Conference
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• 2001.11a
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• pp.19-29
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• 2001

Nutrients are key environmental factors in marine ecosystem. They limit algal growth when at low concentrations and cause algal bloom when at high contents. They also control the growth and succession of many other biota including bacteria and zooplankton, either directly or indirectly. Nutrient contents therefore affect both the structure and functions of marine ecosystem. To study the contents and distribution of nutrients in Chinese Bohai Sea, two cruise surveys were undertaken in August 2000 (summer) and January 2001 (winter), respectively. A total of 595 water samples were collected from 91 sites. After collection the samples were transported to the laboratory and five nutrients, i.e., nitrate, nitrite, ammonia, phosphate and silicate, were analyzed. The results showed that tile average concentration of total inorganic nitrogen (TIN) in Bohai Sea in winter (6.5293.717 ${\mu}$mol$.$l$\^$-1/) was significantly higher than that in summer (3.717 ${\mu}$mol$.$l$\^$-1/). The phosphorus concentration in winter (0.660 ${\mu}$mol$.$l$\^$-1/) was also significantly higher than that in summer (0.329 ${\mu}$mol$.$l$\^$-1/). Mean silicate concentration in winter (7.858 ${\mu}$mol$.$l$\^$-1/) was not significantly different from that in summer (7.200 ${\mu}$mol$.$l$\^$-1/). Nutrients also varied considerable among different areas within Bohai Sea. TIN concentration in Laizhou Bay (4.444 ${\mu}$mol$.$l$\^$-1/), for example, was significantly higher than those in Bohai Bay (2.270 ${\mu}$mol$.$l$\^$-1/) and Bohai Straight (2.431 ${\mu}$mol$.$l$\^$-1/), which probably reflects tile discharge of large amounts of nitrogen into Laizhou Bay via Yellow River. The nutrients also showed vertical distribution pattern. In summer, nutrients in bottom layer were generally higher than those in surface and medium layers. In winter, however. nutrients in different layers were not significantly different Compared with historic data, TIN contents increased continuously since early 1980s, phosphorus arid silicone contents, nevertheless, fell down to some degree. Based on atomic ratios of different nutrients, nitrogen is still the main limiting factor for algal growth in Bohai Sea.

• Bulletin of the Korean Mathematical Society
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• v.50 no.3
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• pp.727-730
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• 2013

We prove an inequality between topological entropy and asymptotical growth of periodic orbits for $C^1$ generic diffeomorphisms.

• Journal of applied mathematics & informatics
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• v.19 no.1_2
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• pp.191-201
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• 2005

In this paper, the existence of periodic positive solution and the attractivity are investigated for the rational recursive sequence $x_{n+1} = (A + ax_{n_k})/(b + x{n-l})$, where A, a and b are real numbers, k and l are nonnegative integer numbers.

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.114-114
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• 2019

• Computers and Concrete
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• v.30 no.4
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• pp.237-242
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• 2022

This paper is concerned with exponential stability in mean square for stochastic static neutral neural networks with varying delays. By using Lyapunov functional method and with the help of stochastic analysis technique, the sufficient conditions to guarantee the exponential stability in mean square for the neural networks are obtained and some results of related literature are extended.

• Journal of Navigation and Port Research
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• v.39 no.5
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• pp.423-429
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• 2015

The expansion on the scale of international trade and rapid economic growth in Northeast Asia have caused intense competition among global companies for securing international logistics center in Northeast Asia. Analyzing location competitiveness for international logistics center in Northeast Asia, this paper aims to extract implications for enhancing locational competitiveness. Employing the relative importance among location selection factors extracted from AHP analysis in prior study, we evaluated the major five port-cities in Northeast Asia including Busan, Gwangyang, Shanghai, Qingdao and Tokyo. In the evaluations, Shanghai (3.926) ranked as the first, followed by Busan (3.859), Qingdao (3.555), Tokyo (3.013) and Gwangyang (2.915). Furthermore, the causal relationships between determinants for location choice decision (logistics factor, cost factor, market factor, service factor and environmental factor) and dependent variables (competitiveness of international logistics center, potentiality to growth, present intention to move into and future plan to move into) were analyzed to provide implications. Results provide useful insights for further improvements, and helps strategic agenda for future development of port-cities.

• Geomechanics and Engineering
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• v.12 no.1
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• pp.35-52
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• 2017

In order to evaluate the grouting effects of water-rich fault in tunnels systematically, a feasible and scientific method is introduced based on the extension theory. First, eight main influencing factors are chosen as evaluation indexes by analyzing the changes of permeability, mechanical properties and deformation of surrounding rocks. The model of evaluating grouting effects based on the extension theory is established following this. According to four quality grades of grouting effects, normalization of evaluation indexes is carried out, aiming to meet the requirement of extension theory on data format. The index weight is allocated by adopting the entropy method. Finally, the model is applied to the grouting effects evaluation in water-rich fault F4-4 of Qingdao Jiaozhou Bay Subsea Tunnel, China. The evaluation results are in good agreement with the test results on the site, which shows that the evaluation model is feasible in this field, providing a powerful tool for systematically evaluating the grouting effects of water-rich fault in tunnels.

• Bulletin of the Korean Chemical Society
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• v.25 no.5
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• pp.673-675
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• 2004

The tetranuclear copper(II) cluster compound $[Cu_4OCl_6(C_{14}H_{12}N_2)_4]$ has been synthesized by hydrothermal reaction and studied by X-ray diffraction. The four copper(II) atoms locate four capsheaves of a tetrahedral skeletal structure and a oxygen atom as interstitial atom occupies the center position of the same tetrahedron, and each edge of the Cu-Cu tetrahedron is bridged by one ${\mu}_2$-Cl anion. The copper atom possesses slightly distorted trigonal bipyramidal geometry with three ${\mu}_2$-Cl atoms in equatorial position and the interstitial O atom and one N atom from 3-benzyl-benzimidazole ligand occupying axial position. The Cu-Cu distances are in the range of 3.0986-3.1162 ${\AA}$. The EPR spectrum suggested that the copper(II) ground state $d_{x2-y2}$ and the coordination geometry was trigonal bipyramidal.

• Steel and Composite Structures
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• v.36 no.5
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• pp.493-506
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• 2020

The load-slip relationship of the shear connection is an important parameter in design and analysis of composite structures. In this paper, a load-slip curve prediction method of the shear connection based on the artificial neural networks (ANNs) is proposed. The factors which are significantly related to the structural and deformation performance of the connection are selected, and the shear stiffness of shear connections and the transverse coordinate slip value of the load-slip curve are taken as the input parameters of the network. Load values corresponding to the slip values are used as the output parameter. A twolayer hidden layer network with 15 nodes and 10 nodes is designed. The test data of two different forms of shear connections, the stud shear connection and the perforated shear connection with flange heads, are collected from the previous literatures, and the data of six specimens are selected as the two prediction data sets, while the data of other specimens are used to train the neural networks. Two trained networks are used to predict the load-slip curves of their corresponding prediction data sets, and the ratio method is used to study the proximity between the prediction loads and the test loads. Results show that the load-slip curves predicted by the networks agree well with the test curves.