• 한국환경보건학회:학술대회논문집
• /
• 한국환경보건학회 2001년도 Proceedings of the 3rd Annual Meeting of Yellow Sea Environment
• /
• pp.17-18
• /
• 2001

• Journal of Microbiology and Biotechnology
• /
• 제20권8호
• /
• pp.1243-1250
• /
• 2010

A cDNA encoding a cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc:153129 from Danio rerio, cystatin B from Theromyzon tessulatum, and the ChainA, stefin B tetramer from Homo sapiens, respectively. The gene was cloned into the intracellular expression vector pET-15b and expressed in Escherichia coli. The recombinant CPI (PA-CPI) was purified by affinity chromatography on Ni-charged resin and ion-exchange chromatography on DEAE-Sepharose FF. The relative molecular mass of PA-CPI was 16 kDa as deduced by SDS-PAGE. Activity analysis showed that the recombinant protein could inhibit the proteolytic activity of papain. A constitutive and secretive expression vector was also constructed, and the cDNA encoding CPI was subcloned into the vector for extracellular expression. Western blotting analysis results showed that the PA-CPI was secreted into the medium. Bioassay demonstrated that E. coli DH5${\alpha}$ harboring pUC18ompAcat-CPI showed a significant difference in mortality to the Asian longhorned beetle Anoplophora glabripennis compared with untransformed E. coli DH5${\alpha}$ and control.

• Journal of Ginseng Research
• /
• 제44권5호
• /
• pp.747-755
• /
• 2020

Background: Ginsenosides accumulation responses to temperature are critical to quality formation in cold-dependent American ginseng. However, the studies on cold requirement mechanism relevant to ginsenosides have been limited in this species. Methods: Two experiments were carried out: one was a multivariate linear regression analysis between the ginsenosides accumulation and the environmental conditions of American ginseng from different sites of China and the other was a synchronous determination of ginsenosides accumulation, overall DNA methylation, and relative gene expression in different tissues during different developmental stages of American ginseng after experiencing different cold exposure duration treatments. Results: Results showed that the variation of the contents as well as the yields of total and individual ginsenosides Rg1, Re, and Rb1 in the roots were closely associated with environmental temperature conditions which implied that the cold environment plays a decisive role in the ginsenoside accumulation of American ginseng. Further results showed that there is a cyclically reversible dynamism between methylation and demethylation of DNA in the perennial American ginseng in response to temperature seasonality. And sufficient cold exposure duration in winter caused sufficient DNA demethylation in tender leaves in early spring and then accompanied the high expression of flowering gene PqFT in flowering stages and ginsenosides biosynthesis gene PqDDS in green berry stages successively, and finally, maximum ginsenosides accumulation occurred in the roots of American ginseng. Conclusion: We, therefore, hypothesized that cold-induced DNA methylation changes might regulate relative gene expression involving both plant development and plant secondary metabolites in such cold-dependent perennial plant species.

• Bulletin of the Korean Chemical Society
• /
• 제34권2호
• /
• pp.475-481
• /
• 2013

By using ionic liquid 1-hexylpyridinium hexafluorophosphate ($HPPF_6$) based carbon ionic liquid electrode (CILE) as the substrate electrode, a $CoMoO_4$ nanorods and myoglobin (Mb) composite was casted on the surface of CILE with chitosan (CTS) as the film forming material to obtain the modified electrode (CTS/$CoMoO_4$-Mb/CILE). Spectroscopic results indicated that Mb retained its native structures without any conformational changes after mixed with $CoMoO_4$ nanorods and CTS. Electrochemical behaviors of Mb on the electrode were carefully investigated by cyclic voltammetry with a pair of well-defined redox peaks from the heme Fe(III)/Fe(II) redox center of Mb appeared, which indicated that direct electron transfer between Mb and CILE was realized. Electrochemical parameters such as the electron transfer number (n), charge transfer coefficient (${\alpha}$) and electron transfer rate constant ($k_s$) were estimated by cyclic voltammetry with the results as 1.09, 0.53 and 1.16 $s^{-1}$, respectively. The Mb modified electrode showed good electrocatalytic ability toward the reduction of trichloroacetic acid in the concentration range from 0.1 to 32.0 mmol $L^{-1}$ with the detection limit as 0.036 mmol $L^{-1}$ ($3{\sigma}$), and the reduction of $H_2O_2$ in the concentration range from 0.12 to 397.0 ${\mu}mol\;L^{-1}$ with the detection limit as 0.0426 ${\mu}mol\;L^{-1}$ ($3{\sigma}$).

• 한국노년학
• /
• 제38권3호
• /
• pp.453-466
• /
• 2018

본 연구의 목적은 정책 비교를 통한 한국의 장기요양보험 제도가 중국 청도의 장기요양보험 시스템 구축에 미치는 영향과 시사점을 검토하는데 있다. 중국과 한국의 문화배경, 생활습관 및 인구구조 등 다양한 측면에서 매우 유사하여 한국 장기요양보험의 성공적인 경험은 중국 청도의 장기요양보험 제도를 구축하는 데 큰 도움이 될 것으로 판단된다. 이에 본 연구는 문헌연구를 통해 Gilbert & Terrell의 사회복지정책분석 프레임워크에서 청도와 한국의 장기요양보험 정책을 비교해 보았다. 정책비교를 통해 청도의 현재 시범 정책 문제점들로 입법 지원 부족, 재정 독립 그리고 심사 기준 등이 명확하지 않고 인적자원 부족을 논의하였다. 이에 다양한 차원에서의 정책비교를 통해 법제 지원, 평가기준 상세화, 혜택범주 확대화, 서비스네트워크 강화, 재원 최적화를 위해 청도의 장기요양보험 개선 제안들을 결론으로 제시해 보았다.

• Nuclear Engineering and Technology
• /
• 제55권8호
• /
• pp.2812-2822
• /
• 2023

The supercritical carbon dioxide (sCO₂) Brayton power cycle is more effective than the conventional power cycle and is more widely applicable to heat sources. The inlet working conditions of the compressor have a higher influence on their operating performance because the thermophysical properties of the CO₂ vary dramatically close to the critical point. The flow in the sCO₂ compressor is simulated and the compressor performance is analyzed. The results show that the sCO₂ centrifugal compressor operates outside of its intended parameters due to the change in inlet temperature. The sCO₂ compressor requires more power as the inlet temperature increases. The compressor power is 582 kW when the inlet temperature is at 304 K. But the power is doubled when the inlet temperature increases to 314 K, and the change in the isentropic efficiency is within 5%. The increase in the inlet temperature significantly reduces the risk of condensation in centrifugal compressors. When the heat load of the sCO₂ power system changes, the inlet pressure to the turbine can be kept constant by regulating the rotational speed of compressors. With the increase in rotational speed, the incidence loss and condensation risk increase.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권9호
• /
• pp.3747-3752
• /
• 2015

Background: Colorectal cancer is one of the most commonly occurring cancers in China. Dietary fibre has been thought to decrease the risk of colorectal cancer in Western countries. However, studies investigating the association between dietary fibre (particularly soluble and insoluble fibres) and colorectal cancer have hitherto been lacking in China. Objective: This case-control study examined the effect of dietary fibre intake on the risk of colorectal cancer, stratified by tumour site. Materials and Methods: The study included 265 cases (colon cancer, 105; rectal cancer, 144; colon and rectal cancer, 16) and 252 controls residing in Qingdao. A food frequency questionnaire that included 121 food items was used to collect dietary information. Odds ratio (OR) and 95% confidence intervals (CI) were calculated using unconditional logistic regression analysis. Results: For food groups, controls in the study consumed more vegetables, soy food and total fibre than did colorectal cancer patients (p<0.05). The intakes of fruit, meat and sea-food did not differ significantly between cases and controls. However, we did not find any association between soy food intake and colon cancer. We observed inverse associations between total fibre intake and colorectal, colon and rectal cancer (Q4 vs Q1: OR=0.44, 95%CI, 0.27-0.73; OR=0.40, 95%CI, 0.21-0.76; OR=0.52, 95%CI, 0.29-0.91). Vegetable fibre intake showed similar inverse associations (Q4 vs Q1: OR=0.51, 95%CI, 0.31-0.85; OR=0.48, 95%CI, 0.25-0.91; OR=0.53, 95%CI, 0.29-0.97). In addition, inverse associations were observed between soluble fibre and insoluble fibre and both colorectal cancer and colon cancer. No relationship was found between colorectal cancer and fruit, soy or grain fibre intakewhen the results were stratified by tumour site. Conclusions: The present study suggests that vegetable fibre and total fibre play very important roles in protecting against colorectal cancer. Soluble and insoluble fibres were inversely associated with only colorectal cancer and colon cancer.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권4호
• /
• pp.1197-1201
• /
• 2012

Objective: To study the relationship between clinical pathologic characteristics, treatment modalities and prognostic factors in HER-2 (Human Epidermal growth factor Receptor-2) overexpressed breast carcinoma. Materials and Methods: Major clinico-pathological factors including therapeutic modalities and survival status of 371 breast cancer patients with HER2 over-expression, teated at Yantai Yuhuangding Hospital from March of 2002 to December of 2010 were retrospectively studied, with special attention focused on survival-related factors. Results: The median age of the total 371 patients in this study was 48 years at time of diagnosis, among which, the leading pathological type was infiltrating ductal carcinoma (92.5%); 62.8% presented with a primary tomor larger than 2 cm in diameter at diagnosis, 51.0% had axillary lymph node (ALN) metastases; ER (Estrogen receptor)/PR (Progesterone receptor) double negative occured in 52.8% of cases, and PCNA (proliferation cell nuclear antigen) (+++) was found in 55.1%. HER-2 overexpressed patients were usually in advanced stage when the diagnosis was made (72.8% at stages IIA~IIIC). The prognosis and survival were assessed in 259 patients with complete follow-up data. 5-year DFS (disease-free survival) and OS (overall survival) rate was 68.0% and 78.0% respectively. Univariate analysis revealed that age, tumor size, ALN metastases, LVSI (lymph-vascular space involvement), PCNA status, hormonal therapy, chemotherapy cycles, and HER-2 overexpression, correlated closely with the prognosis. ALN metastases, LVSI, PCNA status and chemotherapy cycles were independent predictors of survival. Conclusions: HER-2 overexpressed breast cancer has special clinical and pathological characteristics, with advanced clinical stages and high rate of ER/PR double negative. Lymph node metastases, LVSI, PCNA and chemotherapy cycles are independent predictors of prognosis.

• 한국산학기술학회논문지
• /
• 제20권12호
• /
• pp.671-677
• /
• 2019

칭다오의 근대 도시계획은 20세기 초 독일 식민시기 부터 시작되었다. 당시 독일인들은 칭다오 계획에서 근대 도시계획 기법과 도시 건설 기준을 채택했다. 비록 독일 식민시기 이후에도 칭다오의 도시계획은 일본 점령시기와 중화민국 자치정부 시기를 거치면서 도시 계획이 끊임없이 바뀌는 과정을 겪기는 하였으나, 초기 독일인들에 의해 수립된 도시계획의 기본 골격은 지속적으로 유지되었다. 이러한 측면에서 칭다오의 도시계획 발전사는 여러 관점에서 높은 연구 가치를 지니고 있다. 따라서 본 논문은 칭다오의 도시 계획적 특징을 시기별로 구분하여 특징을 분석하고자 한다. 먼저 청도 식민시대의 도시계획에 대한 시간 구분을 실시하였다. 시기별 역사적 배경을 결합하여 각종 문헌 자료를 분석하다. 또한 표를 통해 시기별 도시계획 도면 자료등을 정리하여 칭다오 근대 도시계획의 발전의 변화를 뚜렷이 표현하였다. 본 논문은 칭다오 현재 도시계획 분석을 위한 풍부한 기초 자료를 제공 할 것이다. 본 논문은 총 3장으로 구성된다. 제1장은 서론으로 연구 목적과 범위를 서정하여 제2장은 식민시기 칭다오의 도시계획의 과정을 분석하였다. 제3장에서 특징을 정리하여 결론을 맺다.

• Nutrition Research and Practice
• /
• 제17권4호
• /
• pp.780-788
• /
• 2023

BACKGROUND/OBJECTIVES: This study examined the relationship between famine exposure in early life and the risk of type 2 diabetes in adulthood during the 1959-1961 Chinese Famine. SUBJECTS/METHODS: A total of 3,418 individuals aged 35-74 years free of diabetes from two studies in 2006 and 2009 were followed up prospectively in 2009 and 2012, respectively. Famine exposure was classified as unexposed (individuals born in 1962-1978), fetal exposed (individuals born in 1959-1961), child exposed (individuals born in 1949-1958), and adolescent/adult exposed (born in 1931-1948). A logistic regression model was used to assess the relationship between famine exposure and diabetes after adjustment for potential covariates. RESULTS: During a three-year follow-up, the age-adjusted incidence rates of type 2 diabetes were 5.7%, 14.5%, 12.7%, and 17.8% in unexposed, fetal-exposed, child-exposed, and adolescent/adult-exposed groups, respectively (P < 0.01). Relative to the unexposed group, the relative risks (95% confidence interval) for diabetes were 2.15 (1.29-3.60), 1.53 (0.93-2.51), and 1.65 (0.75-3.63) in the fetal-exposed, child-exposed, and adolescent/adult-exposed groups, after controlling for potential covariates. The interactions between famine exposure and obesity, education level, and family history of diabetes were not observed, except for the urbanization type. Individuals living in rural areas with fetal and childhood famine exposure were at a higher risk of type 2 diabetes, with relative risks of 8.79 (1.82-42.54) and 2.33 (1.17-4.65), respectively. CONCLUSIONS: These findings indicate that famine exposure in early life is an independent predictor of type 2 diabetes, particularly in women. Early identification and intervention may help prevent diabetes in later life.