• Proceedings of the Korea Information Processing Society Conference
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• 한국정보처리학회 2002년도 추계학술발표논문집 (상)
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• pp.197-200
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• 2002

최근 분산 컴퓨팅 환경에서 데이터와 서비스의 복제는 통신 비용의 감소, 데이터 가용성 증가, 그리고 단일 서버의 병목현상을 피하기 위해 필수적이다. 기존의 대표적인 복제 프로토콜로 네트워크를 논리적으로 구성하는 Tree quorum 프로토콜과 Grid 프로토콜이 있다. Tree quorum 프로토콜은 최선의 경우 가장 우수한 읽기 성능을 보이는 반면 트리의 높이가 증가할수록 노드의 수가 기하급수적으로 증가한다는 단점을 가지고 있다. Grid 프로토콜은 읽기 동작에 있어 높은 가용성을 가지는 반면 고장이 없는 환경에서도 같은 읽기 및 쓰기 성능을 보이는 단점을 가지고 있다. 따라서 본 논문에서는 기존의 복제 프로토콜이 가지는 문제점을 해결하고, 복제 노드의 추가와 삭제가 보다 용이한 하이브리드 복제 프로토콜을 제안한다. 제안된 복제 프로토콜은 같은 수의 노드를 갖는 tree quorum 프로토콜에 비해 적은 읽기 비용을 가지며, 효율적인 노드의 구성을 통해 기존 복제 프로토콜보다 높은 데이터의 가용성을 가진다.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.226-233
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• 2007

Members of Methylobacterium, referred as pink-pigmented facultative methylotrophic bacteria, are frequently associated with terrestrial and aquatic plants, tending to form aggregates on the phyllosphere. We report here that the production of autoinducer molecules involved in the cell-to-cell signaling process, which is known as quorum sensing, is common among Methylobacterium species. Several strains of Methylobacterium were tested for their ability to produce N-acyl-homoserine lactone (AHL) signal molecules using different indicators. Most strains of Methylobacterium tested could elicit a positive response in Agrobacterium tumefaciens harboring lacZ fused to a gene that is regulated by autoinduction. The synthesis of these compounds was cell-density dependent, and the maximal activity was reached during the late exponential to stationary phases. The bacterial extracts were separated by thin-layer chromatography and bioassayed with A. tumefaciens NTI (traR, tra::lacZ749). They revealed the production of various patterns of the signal molecules, which are strain dependent. At least two signal molecules could be detected in most of the strains tested, and comparison of their relative mobilities suggested that they are homologs of N-octanoyl-$_{DL}$-homoserine lactone ($C_8-HSL$) and N-decanoyl-$_{DL}$-homoserine lactone ($C_{10}-HSL$).

• Journal of Korea Multimedia Society
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• 제22권8호
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• pp.913-922
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• 2019

The Internet of Things (IoT) envisions smart objects collecting and sharing data at a massive scale via the Internet. One challenging issue is how to disseminate data to relevant data consuming objects efficiently. In such a massive IoT network, Mission critical data dissemination imposes constraints on the message transfer delay between objects. Due to the low power and communication range of IoT objects, data is relayed over multi-hops before arriving at the destination. In this paper, we propose a quorum-based adaptive dissemination algorithm (QADA) for the critical data in the monitoring-based applications of massive IoTs. To design QADA, we first design a new stepped-triangular grid structures (sT-grid) that support data dissemination, then construct a triangular grid overlay in the fog layer on the lower IoT layer and propose the data dissemination algorithm of the publish/subscribe model that adaptively uses triangle grid (T-grid) and sT-grid quorums depending on the mission critical in the overlay constructed to disseminate the critical data, and evaluate its performance as an analytical model.

• Natural Product Sciences
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• 제25권2호
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• pp.172-180
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• 2019

Infections with Pseudomonas aeruginosa are difficult to treat not only because it is often associated with multidrug-resistant infections but also it is able to form biofilm. The aim of this study was to evaluate the antibiofilm and anti-Quorum Sensing (QS) activities of Thymbra capitata essential oils (EOs) against Beta Lactamase (BL) producing P. aeruginosa and the reference strain P. aeruginosa 10145. GC/MS analysis showed that thymol (23.25%) is the most dominant compound in T. capitata EOs. The MICs of T. capitata EOs against P. aeruginosa (BL) and P. aeruginosa 10145 were 1.11%. At sub MIC (0.041, 0.014 and 0.0046%), the EOs of T. capitata remarkably inhibited the biofilm formation of both strains tested and complete inhibition of the biofilm formation was reported at 0.041%. The EOs of T. capitata were found to inhibit the swarming motility, aggregation ability and hydrophobic ability of P. aeruginosa (BL) and P. aeruginosa 10145. Interestingly, the EOs of T. capitata reduce the production of three secreted virulence factors that regulated by QS system including pyocyanin, rhamnolipids and LasA protease. The potent antibiofilm and anti-QS activities of T. capitata EOs can propose it as a new antibacterial agent to control pseudomonas infections.

• Journal of Environmental Science International
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• 제30권5호
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• pp.369-378
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• 2021

In this study, we investigated the effects of indole on biofilm formation inhibition in Pantoea agglomerans (P. agglomerans). In the biofilm growth assay, indole inhibited biofilm formation across all the growth time. Depending on biofilm growth stage, indole exhibited biofilm inhibition and anti-bacterial effects on planktonic cells. Through the analysis of the proportion rate between biofilm and Colony Forming Units (CFU) and inhibition rate of indole, we confirmed that depending on the biofilm stage of P. agglomerans, indole treatment timing was more important than the treatment duration. By comparing gene expression rates through rt-qPCR P.agglomerans affected by indole was found to significantly change quorum sensing (pagI/R) and indole transportation (bssS) gene expressions. Throughout all, indole exhibited both antimicrobial and anti-biofilm effects on P. agglomerans. In addition, we confirmed the anti-biofilm effects of indole on mature biofilm. In conclusion, indole as a signal molecule, can exhibit anti-biofilm effects through bacterial quorum sensing inhibition and indole affects. Therefore, indole can regulate biofilm bacteria especially gram-negative opportunistic pathogens.

• Biomolecules & Therapeutics
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• 제29권3호
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• pp.263-267
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• 2021

Periodontal disease is primarily associated with bacterial infection such as dental plaque. Dental plaque, an oral biofilm harboring a complex microbial community, can cause various inflammatory reactions in periodontal tissue. In many cases, the local bacterial invasion and host-mediated immune responses lead to severe alveolar bone destruction. To date, plaque control, non-surgical, and surgical interventions have been the conventional periodontal treatment modalities. Although adjuvant therapies including antibiotics or supplements have accompanied these procedures, their usage has been limited by antibiotic resistance, as well as their partial effectiveness. Therefore, new strategies are needed to control local inflammation in the periodontium and host immune responses. In recent years, target molecules that modulate microbial signaling mechanisms, host inflammatory substances, and bone immune responses have received considerable attention by researchers. In this review, we introduce three approaches that suggest a way forward for the development of new treatments for periodontal disease; (1) quorum quenching using quorum sensing inhibitors, (2) inflammasome targeting, and (3) use of FDA-approved anabolic agents, including Teriparatide and sclerostin antibody.

• Journal of Korea Multimedia Society
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• 제24권8호
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• pp.1101-1113
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• 2021

The rapid growth of sense-and-respond applications and the emerging cloud computing model present a new challenge: providing publish/subscribe middleware as a scalable and elastic cloud service. The publish/subscribe interaction model is a promising solution for scalable data dissemination over wide-area networks. In addition, there have been some work on the publish/subscribe messaging paradigm that guarantees reliability and availability in the face of node and link failures. These publish/subscribe systems are commonly used in information-centric networks and edge-fog-cloud infrastructures for IoT. The IoT has an edge-fog cloud infrastructure to efficiently process massive amounts of sensing data collected from the surrounding environment. In this paper. we propose a quorum-based hierarchical fault-tolerant publish/subscribe systems (QHFPS) to enable reliable delivery of messages in the presence of link and node failures. The QHFPS efficiently distributes IoT messages to the publish/subscribe brokers in fog overlay layers on the basis of proposing extended stepped grid (xS-grid) quorum for providing tolerance when faced with node failures and network partitions. We evaluate the performance of QHFPS in three aspects: number of transmitted Pub/Sub messages, average subscription delay, and subscritpion delivery rate with an analytical model.

• Journal of Korea Multimedia Society
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• 제25권8호
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• pp.1075-1084
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• 2022

Internet of Things(IoT) has become a key available technology for efficiently implementing device to device(D2D) services in various domains such as smart home, healthcare, smart city, agriculture, energy, logistics, and transportation. A lightweight publish/subscribe(Pub/Sub) messaging protocol not only establishes data dissemination pattern but also supports connectivity between IoT devices and their applications. Also, a Pub/Sub broker is deployed to facilitate data exchange among IoT devices. A scalable edge-based publish/subscribe (Pub/Sub) broker overlay networks support latency-sensitive IoT applications. In this paper, we design a hypercube grid quorum(HGQ) for distributed Pub/Sub systems based IoT applications. In designing HGQ, the network of hypercube structures suitable for the publish/subscribe model is built in the edge layer, and the proposed HGQ is designed by embedding a mesh overlay network in the hypercube. As their applications, we propose an HGQ-based mechansim for dissemination of the data of sensors or the message/event of IoT devices in IoT environments. The performance of HGQ is evaluated by analytical models. As the results, the latency and load balancing of applications based on the distributed Pub/Sub system using HGQ are improved.

• Journal of Microbiology and Biotechnology
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• 제34권4호
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• pp.795-803
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• 2024

Microorganisms usually coexist as a multifaceted polymicrobial community in the natural habitats and at mucosal sites of the human body. Two opportunistic human pathogens, Pseudomonas aeruginosa and Staphylococcus aureus commonly coexist in the bacterial infections for hospitalized and/or immunocompromised patients. Here, we observed that autolysis of the P. aeruginosa quorum-sensing (QS) mutant (lasRmvfR) was suppressed by the presence of the S. aureus cells in vitro. The QS mutant still displayed killing against S. aureus cells, suggesting the link between the S. aureus-killing activity and the autolysis suppression. Independent screens of the P. aeruginosa transposon mutants defective in the S. aureus-killing and the S. aureus transposon mutants devoid of the autolysis suppression revealed the genetic link between both phenotypes, suggesting that the iron-dependent metabolism involving S. aureus exoproteins might be central to both phenotypes. The autolysis was suppressed by iron treatment as well. These results suggest that the interaction between P. aeruginosa and S. aureus might be governed by mechanisms that necessitate the QS circuitry as well as the metabolism involving the extracellular iron resources during the polymicrobial infections in the human airway.

• Korean Journal of Microbiology
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• 제50권2호
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• pp.128-136
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• 2014

Acyl homoserine lactone (AHL) lactonase has been proved to be the AHL-degrading enzyme with the highest substrate specificity for AHL molecules and has shown a considerable potential as low-cost and efficient quorum quenching (QQ) technique. However, few studies focused on its inhibitory effect on biofilm formation which is also a quorum sensing (QS)-regulated phenomenon. In this study, QQ activity of six isolates from biofouled reverse osmosis membranes was studied using Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NTL4 as biosensors under various conditions. All of the isolates belonged to the genus Bacillus and showed QQ activity regardless of the acyl chain length or substitution of AHL molecule. The isolates were capable of significantly inhibiting biofilm formation (46.7-58.3%) by Pseudomonas aeruginosa PAO1 and produced heat-sensitive extracellular QQ substances. The LC-MS analysis of the QQ activity of a selected isolate, RO1S-5, revealed the degradation of N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12 AHL) and the production of corresponding acyl homoserine (3-oxo-C12-HS), which indicated the activity of AHL lactonase. The broad AHL substrate range and high substrate specificity suggested that the isolate would be useful for the control of biofilm-related pathogenesis and biofouling in industrial processes.