• Biomolecules & Therapeutics
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• 제1권1호
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• pp.9-19
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• 1993

DA-125, a new anthracycline antibiotic, showed antitumor activity against animal tumors and human tumors. Therefore we studied the cardiotoxic potential of DA-125 in hamsters and rats as a part of safety research, and compared it with that of doxorubicin(DXR). In acute cardiotoxicity test model used hamsters DA-125 was administered intravenously at a dose of 6, 9, 12 mg/kg, and DXR at 3 mg/kg was given. The electrocardiogram(ECG) of hamsters was recorded for 30 minutes after administration. The DA-125 caused slight ECG alterations at a dose of 6 mg/kg. At a dose of 12 mg/kg DA-125 induced moderate to remarkable changes in ECG like decrease of heart rate, widening of PR interval and 07 interval, and A-V block in 3 out of 5 animals. The severity of ECG alteration at 12 mg/kg of DA-125 was similar to that at 3mg/kg of DXR and these changes caused by DA-125 and DXR recovered within 10 minutes after injection. In chronic cardiotoxicity test model used rats, DA-125 was administered intravenously once a week for three weeks at a dose of 6, 9mg/kg and DXR was given at a dose of 6mg/kg. Electrocardiogram was recorded every week from the start of administration to 2 weeks after the last administration and the animals were sacrificed for histological heart examination at 1 week or 2 weeks after the last administration. DA-125 did not cause any abnormal changes in ECG and in histological heart examination due to administration, but DXR caused widening of ST segment, QRS complex, and QT interval from 1 week after administration and these changes were continued to necropsy. These alterations in ECG were accompanied by cardiac histological lesions such as vacuolation in myocardiac cells, interstitial edema and necrosis of myocytes. These results suggest that DA-125 is less cardiotoxic than DXR.

• Smart Structures and Systems
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• 제26권5호
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• pp.657-666
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• 2020

A rotational pulse-echo ultrasonic propagation imager that can inspect cylindrical specimens for material nondestructive evaluations is proposed herein. In this system, a laser-generated ultrasonic bulk wave is used for inspection, which enables a clear visualization of subsurface defects with a precise reproduction of the damage shape and size. The ultrasonic waves are generated by a Q-switched laser that impinges on the outer surface of the specimen walls. The generated waves travel through the walls and their echo is detected by a Laser Doppler Vibrometer (LDV) at the same point. To obtain the optimal Signal-to-Noise Ratio (SNR) of the measured signal, the LDV requires the sensed surface to be at a right angle to the laser beam and at a predefined constant standoff distance from the laser head. For flat specimens, these constraints can be easily satisfied by performing a raster scan using a dual-axis linear stage. However, this arrangement cannot be used for cylindrical specimens owing to their curved nature. To inspect the cylindrical specimens, a circular scan technology is newly proposed for pulse-echo laser ultrasound. A rotational stage is coupled with a single-axis linear stage to inspect the desired area of the specimen. This system arrangement ensures that the standoff distance and beam incidence angle are maintained while the cylindrical specimen is being inspected. This enables the inspection of a curved specimen while maintaining the optimal SNR. The measurement result is displayed in parallel with the on-going inspection. The inspection data used in scanning are mapped from rotational coordinates to linear coordinates for visualization and post-processing of results. A graphical user interface software is implemented in C++ using a QT framework and controls all the individual blocks of the system and implements the necessary image processing, scan calculations, data acquisition, signal processing and result visualization.

• 대한의생명과학회지
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• 제21권3호
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• pp.135-143
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• 2015

Electrocardiogram measures the electric impulses generated by the heart during its cycle. Recently genome-wide association studies on electrocardiogram traits revealed many relevant genetic loci. Therefore, these findings need to be validated and investigated to determine the underlying mechanisms using mouse models. Invasive radiotelemetry has been widely used to record the electrocardiogram in mice because it has several advantages over non-invasive measurements. However, radiotelemetry is expensive and requires complicated surgery. On the other hand, a non-invasive method using 3 electrodes (one for earth) for lead II is easy to establish and allows for rapid measurement. In this study, eleven mice were measured with this non-invasive method and no statistical difference among them was found in any ECG measurements. In addition, repeat measurement in the same mouse was performed in 9 sets of experiment and the results indicated that non-invasive method was reliable for reproducibility. Further it was shown that measurements for 1, 5, 10, and 15 minutes were not different so that a short recording such as 5 minutes was enough to estimate the ECG values including heart rate. Further this method was validated by measuring the ECG of Balb/c and FVB that were previously shown to differ in ECG values by radiotelemetry. Significant differences were found in heart rate, PR interval and corrected QT interval between these mouse strains. This study partially proved that non-invasive method also could provide the accuracy and reproducibility. Based on these results, the non-invasive ECG recordings of lead II is recommended as a useful method for quick test in mouse model.

• Journal of Power Electronics
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• 제16권5호
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• pp.1904-1917
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• 2016

The moving average filter (MAF) is widely utilized to improve the disturbance rejection capability of phase-locked loops (PLLs). This is of vital significance for the grid-integration and stable operation of power electronic converters to electric power systems. However, the open-loop bandwidth is drastically reduced after incorporating a MAF into the PLL structure, which makes the dynamic response sluggish. To overcome this shortcoming, some new techniques have recently been proposed to improve the transient response of MAF-based PLLs. In this paper, a comprehensive performance comparison of advanced MAF-based PLL algorithms is presented. This comparison includes HPLL, MPLC-PLL, QT1-PLL, and DMAF-PLL. Various disturbances, such as grid voltage sag, voltage flicker, harmonics distortion, phase-angle and frequency jumps, DC offsets and noise, are considered to experimentally test the dynamic performances of these PLL algorithms. Finally, an improved positive sequence extraction method for a HPLL under the frequency jumps scenario is presented to compensate for the steady-state error caused by non-frequency adaptive DSC, and a satisfactory performance has been achieved.

• 한국지반신소재학회논문집
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• 제16권1호
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• pp.73-83
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• 2017

모래 및 실트 함유량이 우세한 서해안 저소성 지반(인천, 화성, 군산)에서 실시된 실내 및 현장시험으로부터 얻어진 비배수전단 강도를 이용하여 피에조콘계수(Nkt)를 분석한 후, 이에 대한 적용성을 평가하였다. 인천, 화성 및 군산지역에서 얻어진 일축압축 강도에 의한 콘계수(Nkt)는 19~23, 간이 CU 강도에 의한 값은 13~13.8, 현장베인강도에 의한 값은 11.6~13.1로 평가되었다. 이는 저소성 실트 지반조건에서 일축압축강도가 과소평가되는 원인에 의한 것으로 간이 CU 강도 적용조건과 비교했을 때 콘계수(Nkt)가 약 1.8배 전후로 커지며 분산되는 경향을 나타냈다. 저소성 실트 지반에서 수행된 CPTU 데이터를 이용하여 콘계수(Nkt)를 평가할 때에는 지반의 입도분포, 액소성한계 등의 물리적 특성, 지층 내의 sandseam 분포 등으로 인한 콘선단저항(qt) 및 주면마찰력(fs)의 불규칙한 분포, 간극수압계수(Bq)를 종합적으로 분석하여 수행하는 것이 바람직할 것으로 판단된다.

• The Korean Journal of Physiology and Pharmacology
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• 제14권5호
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• pp.305-310
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• 2010

The human $ether$-$a$-$go$-$go$-related gene ($hERG$) channel is important for repolarization in human myocardium and is a common target for drugs that prolong the QT interval. We studied the effects of two antipsychotics, tiapride and sulpiride, on hERG channels expressed in $Xenopus$ oocytes and also on delayed rectifier $K^+$ currents in guinea pig cardiomyocytes. Neither the amplitude of the hERG outward currents measured at the end of the voltage pulse, nor the amplitude of hERG tail currents, showed any concentration-dependent changes with either tiapride or sulpiride ($3{\sim}300{\mu}M$). However, our findings did show that tiapride increased the potential for half-maximal activation ($V_{1/2}$) of HERG at $10{\sim}300{\mu}M$, whereas sulpiride increased the maximum conductance ($G_{max}$) at 3, 10 and $100{\mu}M$. In guinea pig ventricular myocytes, bath applications of 100 and $500{\mu}M$ tiapride at $36^{\circ}C$ blocked rapidly activating delayed rectifier $K^+$ current ($I_{Kr}$) by 40.3% and 70.0%, respectively. Also, sulpiride at 100 and $500{\mu}M$ blocked $I_{Kr}$ by 38.9% and 76.5%, respectively. However, neither tiapride nor sulpiride significantly affected the slowly activating delayed rectifier $K^+$ current ($I_{Ks}$) at the same concentrations. Our findings suggest that the concentrations of the antipsychotics required to evoke a 50% inhibition of IKr are well above the reported therapeutic plasma concentrations of free and total compound.

• 생명과학회지
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• 제17권2호통권82호
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• pp.204-210
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• 2007

평활근 ${\alpha}$-트로포마이오신의 높은 액틴 친화력은 아미노산 잔기 Gln276 및 Thr277에 기인한다는 이전 보고에 따라, 2 잔기 중 어느 잔기가 액틴 친화력에 더 중요한 가를 알아보기 위하여 골격근 트로포마이오신의 His 혹은 Ala 단일 잔기를 각각 Gln 혹은 Thr으로 치환한 돌연변이 트로포마이오신을 제작하여 대장균에서 대량발현 시킨 후 정제하여 액틴 결합력을 측정하였다. 비록 비아세틸화된 트로포마이오신의 경우 Gln 및 Thr 잔기가 최고 액틴친화력을 위해 모두 필요하나, 돌연변이 트로포마이신 중 Gln 잔기를 가진 돌연변이 트로포마이오신들이 다른 돌연변이 트로포마이오신들에 비하여 3에서 4배 높은 액틴친화력을 보였다. 이러한 결과는 평활근 ${\alpha}$-트로포마이오신의 높은 액틴 친화력은 Thr277 잔기보다 Gln276 잔기에 주로 기인한다는 것을 의미한다.

• Corrosion Science and Technology
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• 제15권2호
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• pp.84-91
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• 2016

The transport and deposition of corrosion products in pressurized water nuclear reactor (PWR) steam generators have led to corrosion (SCC, denting etc.) problems. Lancing, mechanical cleaning and chemical cleaning have been used to reduce these problems. The methods of lancing and mechanical cleaning have limitations in removing corrosion products due to the structure of steam generator tubes. But high temperature chemical cleaning (HTCC) with EDTA is the most effective method to remove corrosion products regardless of the structure. However, EDTA in chemical cleaning aqueous solution and chemical cleaning environments affects the integrity of materials used in steam generators. The nuclear power plants have to perform the pre-test (also called as qualification test (QT)) that confirms the effect on the integrity of materials after HTCC. This is one of the series studies that assess the effect, and this study determines the effects of 20 % EDTA aqueous solution on defective tubes in high temperature chemical cleaning environments. The depth and magnitude of defects in steam generator (SG) tubes were measured by eddy current test (ECT) signals. Surface analysis and magnitude of defects were performed by using SEM/EDS. Corrosion rate was assessed by weight loss of specimens. The ECT signals (potential and depth %) of defective tubes increased marginally. But the lengths of defects, oxides on the surface and weights of specimens did not change. The average corrosion rate of standard corrosion specimens was negligible. But the surfaces on specimens showed traces of etching. The depth of etching showed a range on the nanometer. After comprehensive evaluation of all the results, it is concluded that 20 % EDTA aqueous solution in high temperature chemical cleaning environments does not have a negative effect on defective tubes.

• The Korean Journal of Physiology and Pharmacology
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• 제15권5호
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• pp.291-297
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• 2011

The effect of cyclosporin A (CsA), an immunosuppressant, on human ether-a-go-go-related gene (HERG) channel as it is expressed in human embryonic kidney cells was studied using a whole-cell, patch-clamp technique. CsA inhibited the HERG channel in a concentration-dependent manner, with an $IC_{50}$ value and a Hill coefficient of $3.17{\mu}m$ and 0.89, respectively. Pretreatment with cypermethrine, a calcineurin inhibitor, had no effect on the CsA-induced inhibition of the HERG channel. The CsA-induced inhibition of HERG channels was voltage-dependent, with a steep increase over the voltage range of the channel opening. However, the inhibition exhibited voltage independence over the voltage range of fully activated channels. CsA blocked the HERG channels predominantly in the open and inactivated states rather than in the closed state. Results of the present study suggest that CsA acts directly on the HERG channel as an open-channel blocker, and it acts independently of its effect on calcineurin activity.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2019년도 학술발표회
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• pp.264-264
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• 2019

개수로 흐름 해석을 위해 수치모형을 적용할 때 반드시 거쳐야 하는 과정이 격자망을 구성하는 일이다. 불규칙한 형상의 자연수로를 모의할 때 격자망 생성은 쉬운 일이 아니며, 따라서 가시적으로 격자망 생성을 돕고, 격자망의 수정도 용이한 도구가 요구된다. 따라서 본 연구에서는 수심적분된 흐름방정식을 지배방정식으로 하여 개수로 흐름 해석을 용이하게 하고자 그래픽 사용자 인터페이스(GUI)를 개발하였으며, 이를 소개하고자 한다. 격자망은 기본적으로 사각형과 삼각형 요소로 구성될 수 있으며, 유한차분모형 등에서는 정형사각형 격자망을, 유한요소모형에서는 비정형 사각형 및 삼각형 격자망 또는 혼합망을 생성시킬 수 있다. 이산점(scatter points)이나 절점(node points)을 생성하거나 기존의 자료를 불러들여 삼각망 또는 사각망을 형성시킬 수 있으며, 연속선(polylines)을 작성하여 형성된 폐다각형(polygones)을 이용하여 정규 또는 비정규의 삼각망 또는 사각망을 형성시킬 수 있다. 또한 두 점사이를 선형 또는 반원 형태로 편향 정도(biased value)를 설정하여 원하는 개수만큼 나눌 수 있도록 하여, 보다 효율적인 격자형성이 가능토록 하였다. 기존 상용 프로그램에서 작성된 격자를 불러들여 활용 가능하며, 백그라운드 이미지로 지형도나 위성사진을 띄어놓고 이미지 상에서 격자를 형성할 수도 있다. 기본적으로 마우스를 이용하여 화면의 이동, 확대 및 축소와 점, 선, 요소의 생성 및 선택이 가능하다. 본 프로그램은 Qt와 modern OpenGL을 바탕으로 제작되었으며, 마이크로소프트사의 windows 뿐만 아니라 Mac OS, Linux 버전의 설치 파일 작성이 가능하다.