• International Journal of Advanced Culture Technology
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• 제11권2호
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• pp.152-161
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• 2023

Modern people live a life connected with the real world and the virtual world by relying on the new media of enterprises and social consumption led by innovative technologies. In this environment, virtual influencers actively communicate with consumers and build relationships through social media, which is a new marketing tool that has attracted widespread attention. From a business perspective, it is necessary to have a solid understanding of this phenomenon, and then explore communication strategies to effectively develop virtual influencers. To investigate followers' preference for virtual influencers, this study employs the Q-method, which studies human subjective attributes, an empirical research effort to uncover complex issues in human subjectivity. To determine the factors that trigger people's voluntary and active practice and the preference degree of virtual influencers, the Q method is implemented to examine human subjectivity, thoughts and attitudes. According to the results of this study, virtual influencers are a new group of idols full of vitality. The interviews found that there are still many virtual influencers who do not know about followers, but each type can be clearly understood through the intuitive understanding of the interviewees. Divided out, type 1 one egoideal virtual influencers aim to represent an idealized version of the creator or target audience. Embodies ideal physical characteristics, personality or lifestyle desired by the audience. Type 2 is charismatic and attractive, and has the characteristics of most virtual influencers. It is suggested that it can be developed into a potential type, doing brand cooperation, and content production on social media platforms. Type 3: Game animation, derived from the image of characters in games or comics, with stylized features and energetic personalities, which can be integrated into games or entertainment experiences. Type 4 development potential type is the most successful type among virtual imagers, and it is also the purpose of marketing virtual influencers. It is essential that brand endorsement on social media platforms, integrated marketing, and driving advertising traffic. It is recommended to improve production technology to reduce investment costs.

• 상하수도학회지
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• 제33권4호
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• pp.259-268
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• 2019

One of the most effective methods to consistently ensure the safety of a tap water supply can be achieved by application of a comprehensive risk assessment and risk management approach for drinking water supply systems. This approach can be termed water safety plans(WSP) which recommended by WHO(world health organization) and IWA(international water association). For the introduction of WSP into Korea, 150 hazards were identified all steps in drinking water supply from catchment to consumer and risk assessment tool based on frequency and consequence of hazards were developed. Then, developed risk assessment tool by this research was implemented at a water treatment plant($Q=25,000m^3/d$) to verify its applicability, and several amendments were recommended; classification of water source should be changed from groundwater to stream to strengthen water quality monitoring contaminants and frequencies; installation of aquarium to monitor intrusion of toxic substances into raw water; relocation or new installation on-line water quality analyzers for efficient water quality monitoring; change of chlorination chemical from solid phase($Ca(OCl)_2$) to liquid phase(NaOCl) to improve soundness of chlorination. It was also meaningful to propose hazards and risk assessment tool appropriate for Korea drinking water supply systems through this research which has been inconsistent among water treatment authorities.

• 한국물환경학회지
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• 제34권1호
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• pp.46-56
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• 2018

A mcyB-specific Ultra-Rapid quantitative PCR was developed for the quantitative detection of Microcystis aeruginosa, which is often a dominant species in green tide. McyB-specific UR-qPCR was optimized under extremely short times of each step in thermal cycles, based on the specific primers deduced from the mcyB in microcystin synthetase of M. aeruginosa. The M. aeruginosa strain KG07 was used as a standard for quantification, after the microscopic counting and calculation by mcyB-specific UR-qPCR. The water samples from the river water with the Microcystis outbreak were also measured by using both methods. The $1.0{\times}10^8$ molecules of mcyB-specific DNA was recognized inner 4 minutes after beginning of UR-qPCR, while $1.0{\times}10^4$ molecules of mcyB-specific templates was detected inner 7 minutes with quantitative manner. From the range of $1.0{\times}10^2$ to $1.0{\times}10^8$ initial molecules, quantification was well established based on $C_T$ using mcyB-specific UR-qPCR (Regression coefficiency, $R^2=0.9977$). Between the numbers of M. aeruginosa cell counting under microscope and calculated numbers using mcyB-specific UR-qPCR, some differences were often found. The reasons for these differences were discussed; therefore, easy compensation method was proposed that was dependent on the numbers of the cell counting. Additionally, to easily extract the genomic DNA (gDNA) from the samples, a freeze-fracturing of water-sample using liquid nitrogen was tested, by excluding the conventional gDNA extraction method. It was also verified that there were no significant differences using the UR-qPCR with both gDNAs. In conclusion, the mcyB-specific UR-qPCR that we proposed would be expected to be a useful tool for rapid quantification and easy monitoring of M. aeruginosa in environmental water.

• Design & Manufacturing
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• 제13권2호
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• pp.54-59
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• 2019

CNC(Computer Numerical Control) machine tools are being used in various industrial fields such as aircraft and automobiles. The machining conditions used in the mold industry are used, and the simulation and the experiment are compared. The tool used in the experiment was carried out to increase the reliability of the simulation of the cutting machining. The program used in the 3D-FEM (finite element method) was the AdvantEdge and predicted by down-milling. The tool model is used 3D-FEM simulation by using the cutting force, temperature prediction. In this study, we carried out the verification of cutting force by using a 3-axis tool dynamometer (Kistler 9257B) system when machining the plastic mold Steel machining of NAK-80. The cutting force experiment data using on the charge amplifier (5070A) is amplified, and the 3-axis cutting force data are saved as a TDMS file using the Lab-View based program using on NI-PXIe-1062Q. The machining condition 7 was the most similar to the simulation and the experimental results. The material properties of the NAK-80 material and the simulation trends reflected in the reverse design of the tool were derived similarly to the experimental results.

• 정보처리학회논문지D
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• 제9D권4호
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• pp.677-686
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• 2002

내장형 시스템의 실시간 운영체제(RTOS)에서는 메모리와 CPU 속도 등의 제한적인 환경에서 원하는 기능을 최적으로 최단시간에 구현할 수 있는 개방형 개발도구 환경이 요구된다. 이러한 개방형 개발환경에는 타겟 시스템에 최소의 부담을 주면서 사용자가 편리하게 원하는 정보를 원격지에서 대화식으로 빠르게 접근, 참조, 제어 할 수 있는 원격 대화형 쉘이 필수적이다. 본 논문에서는 원격지에서 모듈별 로딩, 태스크의 스폰과 더불어 태스크 상태 등을 확인할 수 있는 원격 대화형 쉘 프로그램의 설계와 그에 대한 구현방법을 기술하고 원격지에서 타겟의 부담을 최소화하여 실시간 운영체계에서 정보접근이 용이하고 유지보수가 쉬운 개발환경이 가능하도록 하였다. 이 대화형 쉘은 Q-PLUS RTOS와 연동되어 작동하며 ARM계열의 EBSA285 타겟보드와 NT 호스트상에서 구현, 테스트되었다.

• 한국식품위생안전성학회지
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• 제33권1호
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• pp.23-30
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• 2018

본 논문에서는 유통 농산물 잔류농약 부적합 현황 분석을 위하여 네트워크 분석 기법을 적용하였다. 분석용 데이터는 "2017년도 식품안전관리지침"내 "유통농산물별 잔류농약 부적합 현황"을 참고하였으며, 주요 분석 기법으로는 중심성 분석(연결정도 중심성, 근접 중심성, 매개 중심성)을 적용하였다. 연결정도 중심성 분석결과 chlorpyrifos와 diazinon에서 "연결된 노드"가 가장 많은 것으로 확인되었다. 근접 중심성에서는 19종을 제외한 농약성분들 사이에서 유사한 정도를 보여주고 있었다. 매개 중심성 분석 결과에서는 fluioxonil과 chlorpyrifos가 가장 높은 경향을 보여주어 이들이 농약성분 네트워크에서 "가교" 역할을 수행 하는 것으로 파악되었다. 네트워크 분석 결과, 기존의 통계분석을 통해서는 분석 할 수 없었던 농약성분들 사이의 "관계" 데이터를 확인 할 수 있었다. 향후 이러한 분석기법은 최적화/정교화 과정을 통해서 보다 정밀하게 농약성분 부적합 현황 분석을 위한 도구로서 적용되리라 기대한다.

• Journal of Veterinary Science
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• 제22권6호
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• pp.87.1-87.12
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• 2021

Background: African swine fever virus (ASFV), classical swine fever virus (CSFV), and porcine reproductive and respiratory syndrome virus (PRRSV) are still prevalent in many regions of China. Co-infections make it difficult to distinguish their clinical symptoms and pathological changes. Therefore, a rapid and specific method is needed for the differential detection of these pathogens. Objectives: The aim of this study was to develop a multiplex real-time quantitative reverse transcription polymerase chain reaction (multiplex qRT-PCR) for the simultaneous differential detection of ASFV, CSFV, and PRRSV. Methods: Three pairs of primers and TaqMan probes targeting the ASFV p72 gene, CSFV 5' untranslated region, and PRRSV ORF7 gene were designed. After optimizing the reaction conditions, including the annealing temperature, primer concentration, and probe concentration, multiplex qRT-PCR for simultaneous and differential detection of ASFV, CSFV, and PRRSV was developed. Subsequently, 1,143 clinical samples were detected to verify the practicality of the assay. Results: The multiplex qRT-PCR assay could specifically and simultaneously detect the ASFV, CSFV, and PRRSV with a detection limit of 1.78 × 10⁰ copies for the ASFV, CSFV, and PRRSV, but could not amplify the other major porcine viruses, such as pseudorabies virus, porcine circovirus type 1 (PCV1), PCV2, PCV3, foot-and-mouth disease virus, porcine parvovirus, atypical porcine pestivirus, and Senecavirus A. The assay had good repeatability with coefficients of variation of intra- and inter-assay of less than 1.2%. Finally, the assay was used to detect 1,143 clinical samples to evaluate its practicality in the field. The positive rates of ASFV, CSFV, and PRRSV were 25.63%, 9.36%, and 17.50%, respectively. The co-infection rates of ASFV+CSFV, ASFV+PRRSV, CSFV+PRRSV, and ASFV+CSFV+PRRSV were 2.45%, 2.36%, 1.57%, and 0.17%, respectively. Conclusions: The multiplex qRT-PCR developed in this study could provide a rapid, sensitive, specific diagnostic tool for the simultaneous and differential detection of ASFV, CSFV, and PRRSV.

• 한국수자원학회논문집
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• 제54권10호
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• pp.819-833
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• 2021

본 연구는 안동댐 하류(4,565.7 km²)를 대상으로 준분포형 수문모델인 SWAT과 서식처모델인 PHABSIM을 연계하여 적용성을 평가하고, 대상어종에 대해 환경생태유량을 산정하였다. 대상유역 내 다목적 댐 2개(안동댐: ADD, 임하댐: IHD)의 실제운영자료를 구축하여 SWAT에 적용하였으며 추가로 1개의 수위관측소(구담: GD)을 선정하여 ADD, IHD, GD에 대해 유입·유출량 검보정을 수행하였다. 검보정 결과 R²는 0.52 ~ 0.74, NSE는 0.48 ~ 0.71, RMSE는 0.92 ~ 2.51 mm/day로 분석되었다. 검보정된 GD의 유출량을 이용하여 2012년부터 2020년까지의 유황분석을 실시한 결과, 관측값의 유황분석 결과와 비교하여 평균 Q185는 36.5 m³/sec (-1.4%), 평균 Q275는 23.8 m³/sec (0%)로 분석되어 Q185와 Q275를 PHBASIM의 유량 경계조건으로 적용하였다. PHABSIM의 대상하천은 GD가 위치한 410 m 구간으로 선정하였고 하천기본계획보고서 기반으로 하천단면 및 수리인자를 구축하였다. 대상하천의 우점종은 피라미, 아우점종은 돌고기로 분석되었으며 문헌조사를 통해 대상어종의 HSI를 수집하였다. PHABSIM 수위 모의결과, Q185, Q275에서 각각 -0.12, +0.00 m의 오차가 발생하였고, 유속모의의 경우 Q185, Q275에서 각각 +0.06, +0.09 m/s의 오차가 발생하여 연계모의가 적절하게 된 것을 확인하였다. PHABSIM의 서식처 모의 결과, 피라미의 평균 가중가용면적 WUA와 환경생태유량은 76,817.0 m²/1000m, 20.0 m³/sec, 돌고기의 평균 WUA와 환경생태유량은 46,628.6 m²/1000m, 9.0 m³/sec로 분석되어 피라미가 돌고기보다 대상하천에 대해 적응성이 높은 것을 확인할 수 있었다.

• 대한토목학회논문집
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• 제13권5호
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• pp.13-18
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• 1993

본 논문의 목적은 LRB 기초분리장치가 설치된 교량의 내진효과를 해석하는 것이다. 기초분리방법은 지진력이 구조물에 전달되기 전에 그 영향을 최소화시키는 방법으로 현재 건물, 발전소, 교량 등의 구조물에 실용화되고 있다. 본 연구에서는 대표적인 기초분리장치인 Lead Rubber Bearing이 설치된 교량의 단순화된 모델을 개발하고 여러 크기의 LRB에 대하여 지진의 영향을 해석하였다. 단순화된 교량모델의 운동방정식을 Newmark ${\beta}$ 방법에 의해 시간이력해석을 수행하였다. 기초분리장치의 모델로는 이중선형성을 갖는 스프링을, 교각모델로는 비선형성 및 강성저하를 고려한 Q-HYST모델을 사용하였다. 해석예로 미국 네바다주에 소재한 Rose Creek 교량에 대한 남북방향 El Centro지진(1940)의 영향을 해석하였다. 상부구조 횡방향처짐, 교각연성 및 교각 밑면 전단력을 구하였다.

• Archives of Pharmacal Research
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• 제21권3호
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• pp.305-309
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• 1998

Human cytochrome P450 1A2 is one of the major cytochrome P450s in human liver. It is known to be capable of activating a number of carcinogens such as arylamines and heterocyclic amines. In order to develop the new bacterial mutagenicity test system with human P450, a full length of human P450 1A2 cDNA inserted into pCW bacterial expression vector was introduced to Escherichia coli WP2 uvrA strain which is a well-known E. coli strain for bacterial reverse mutagenicity assay. Expressed human P450 1A2 showed typical P450 hemoprotein spectra. Maximum expression was achieved at 48 hrs after incubating at $30^{\circ}C$ in terrific broth containing ampicillin, IPTG and other supplements. High level expression of P450 1A2 in E. coli WP2 uvrA membranes was determined in SDS-PAGE. The well-known mutagens 2-aminoanthracene and MElQ increased the revertant colonies of E. coli WP2 uvrA expressing human P450 1A2 without an exogenous rat hepatic post-mitochondrial supernatant (S9 fraction) in a dose-dependent manner. The results show that the functional expression of human P450 in bacterial mutagenicity tester strain will provide a useful tool for studying the mechanism of the mutagenesis and carcinogenesis of new drugs and environmental chemicals.