• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1358-1365
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• 2021

To clarify the role of long intergenic nonprotein-coding RNA 1232 (LINC01232) in the progression of gastric cancer and the potential mechanism, we analyzed the expression of LINC01232 in TCGA database using the GEPIA online tool, and the LINC01232 level in gastric cancer cell lines was detected by quantitative real time-polymerase chain reaction (qRT-PCR) as well. Cell proliferation assay, colony formation assay, transwell assay and tumor formation experiment in nude mice were conducted to observe the biological behavior changes of gastric cancer cells through the influence of LINC01232 knockdown. LncATLAS database and subcellular isolation assay were used for subcellular distribution of LINC01232 in gastric cancer cells. The interaction among LINC01232, zeste homolog 2 (EZH2) and kruppel-like factor 2 (KLF2) was clarified by RNA-protein interaction prediction (RPISeq), RNA immunoprecipitation (RIP), qRT-PCR and chromatin immunoprecipitation (ChIP) assay. Rescue experiments were further conducted to elucidate the biological function of LINC01232/KLF2 axis in the progression of gastric cancer. LINC01232 was upregulated in stomach adenocarcinoma (STAD) tissues and gastric cancer lines. LINC01232 knockdown inhibited the proliferative capacities of gastric cancer cells in vitro, and impaired in vivo tumorigenicity. LINC01232 was mainly distributed in the cell nucleus where it epigenetically repressed KLF2 expression via binding to the enhancer of EZH2, which was capable of binding to promoter regions of KLF2 to induce histone H3 lysine 27 trimethylation (H3K27me3). LINC01232 exerts oncogenic activities in gastric cancer via inhibition of KLF2, and therefore, the knockdown of KLF2 could reverse the regulatory effect of LINC01232 in the proliferative ability of gastric cancer cells.

• Journal of Genetic Medicine
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• 제15권2호
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• pp.64-71
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• 2018

Purpose: Overgrowth syndromes are conditions that involve generalized or localized areas of excess growth. In this study, the clinical, molecular, and genetic characteristics of Korean patients with overgrowth syndrome were analyzed. Materials and Methods: We recruited 13 patients who presented with overgrowth syndrome. All patients fulfilled inclusion criteria of overgrowth syndrome. Analysis of the clinical and molecular investigations of patients with overgrowth syndrome was performed retrospectively. Results: Among the 13 patients with overgrowth syndrome, 9 patients (69.2%) were found to have molecular and genetic causes. Among the seven patients with Sotos syndrome (SS), two had a 5q35microdeletion that was confirmed by fluorescent in situ hybridization. In two patients with SS, intragenic mutations including a novel mutation, c.5993T>A (p.M1998L), were found by Sanger sequencing. One patient had one copy deletion of NDS1 gene which was confirmed by multiplex ligation-dependent probe amplification. Among five patients with Beckwith-Wiedemann syndrome, three had aberrant imprinting control regions; 2 hypermethylation of the differentially methylated region of H19, 1 hypomethylation of the differentially methylated region of Kv. In one patient displaying overlapping clinical features of SS, a de novo heterozygous deletion in the chromosomal region 7q22.1-22.3 was found by single nucleotide polymorphism-based microarray. Conclusion: Considering high detection rate of molecular and genetic abnormalities in this study, rigorous investigations of overgrowth syndrome may be an important tool for the early diagnosis and genetic counseling. A detailed molecular analysis of the rearranged regions may supply the clues for the identification of genes involved in growth regulation.

• 보건행정학회지
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• 제29권2호
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• pp.146-159
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• 2019

Background: The purpose of this study is to measure the Korean health literacy level and to analyse its gaps according to the factors of socioeconomic and health status and health behaviors. Based on this, policy implications were reviewed to improve the understandability on health information and to reduce the gap among socioeconomic groups. Methods: HLS-EU-Q47, a tool developed by the European Health Literacy Project, was used to conduct a face-to-face interview survey on the health literacy for the samples from general population. Results: The public general health literacy (HL) index was 34.5 out of 50. HL is consisted of three sub-dimensions: healthcare (HC-HL), disease prevention (DP-HL), and health promotion (HP-HL). And a HL analysis found scores of 34.7 points for HC-HL, 35.4 points for DP-HL, and 33.3 points for HP-HL. The level of all HL was different according to socioeconomic characteristics and health behavior. Conclusion: Based on the results of this study, more research activities on health-related literacy need to be conducted, and monitoring system on the HL level needs to be developed and implemented. In addition, a program to improve HL levels needs to be developed in order to strengthen the basis for a more sustainable healthcare system as an agenda with national health policy priority.

• 천문학논총
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• 제34권1호
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• pp.1-16
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• 2019

"Hon-Chon-Jeon-Do" is a woodcut star map with the size of $79.4cm{\times}127.5cm$, and was widely disseminated as it was made into a set with Kim, Jung Ho's "Yeoji-Jeon-Do". This study confirmed that Yixiang kaocheng xubian ("의상고성속편") star catalogue was used as a source to produce the star map, and the stereographic projection was applied with the projection center being the mid-point (Q) between the celestial and ecliptic north poles. The 'mid-circle' around the Q is arisen between the equator and the ecliptic, and on this circle, the hour angle and the ecliptic longitude of a star can be marked using the same scale. This means that the hour of the day and the season of the year can be read on the same dial of the mid-circle, and the application of this character in the practical use was the key point of the star map production. By observing either transits or positions of the 28 xiu (宿), it is easy to find the corresponding season and time by simply reading the dial on the mid-circle. This is just the function of a portable almanac and thus by disseminating it widely, the convenience of the people would have been promoted. For this reason, it can be stated that "Hon-Chon-Jeon-Do" was a practical astronomical tool which was produced by the western astronomical projection method and was used to find time and season. Choi, Han Ki and Kim, Jung Ho are strong candidates for the makers of this star map. The time of production is estimated to be 1848 ~ 1857, and "Hon-Chon-Jeon-Do" could be regarded as a good contributor to popularization of astronomy in the late Joseon Dynasty.

• Animal Bioscience
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• 제34권1호
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• pp.134-142
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• 2021

Objective: To understand the athletic characteristics of Thoroughbreds, high-throughput analysis has been conducted using horse muscle tissue. However, an in vitro system has been lacking for studying and validating genes from in silico data. The aim of this study is to validate genes from differentially expressed genes (DEGs) of our previous RNA-sequencing data in vitro. Also, we investigated the effects of exercise-induced stress including heat, oxidative, hypoxic and cortisol stress on horse skeletal muscle derived cells with the top six upregulated genes of DEGs. Methods: Enriched pathway analysis was conducted using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) tool with upregulated genes in horse skeletal muscle tissue after exercise. Among the candidates, the top six genes were analysed through geneMANIA to investigate gene networks. Muscle cells derived from neonatal horse skeletal tissue were maintained and subjected to exercise-related stressors. Transcriptional changes in the top six genes followed by stressors were investigated using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: The inflammation response pathway was the most commonly upregulated pathway after horse exercise. Under non-cytotoxic conditions of exercise-related stressors, the transcriptional response of the top six genes was different among types of stress. Oxidative stress yielded the most similar expression pattern to DEGs. Conclusion: Our results indicate that transcriptional change after horse exercise in skeletal muscle tissue strongly relates to stress response. The qRT-PCR results showed that stressors contribute differently to the transcriptional regulation. These results would be valuable information to understand horse exercise in the stress aspect.

• Entomological Research
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• 제48권5호
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• pp.390-399
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• 2018

In order to precisely assess gene expression levels, the suitable internal reference genes must be served to quantify real-time reverse transcription polymerase chain reaction (RT-qPCR) data. For armyworm, Mythimna separata, which reference genes are suitable for assessing the level of transcriptional expression of target genes have yet to be explored. In this study, eight common reference genes, including ${\beta}$-actin (${\beta}$-ACT), 18 s ribosomal (18S), 28S ribosomal (28S), glyceraldehyde-3-phosphate (GAPDH), elongation fator-alpha ($EF1{\alpha}$), TATA box binding protein (TBP), ribosomal protein L7 (RPL7), and alpha-tubulin (${\alpha}$-TUB) that in different developmental stages, tissues and insecticide treatments of M. separata were evaluated. To further explore whether these genes were suitable to serve as endogenous controls, three software-based approaches (geNorm, BestKeeper, and NormFinder), the delta Ct method, and one web-based comprehensive tool (RefFinder) were employed to analyze and rank the tested genes. The optimal number of reference genes was determined using the geNorm program, and the suitability of particular reference genes was empirically validated according to normalized HSP70, and MsepCYP321A10 gene expression data. We found that the most suitable reference genes for the different experimental conditions. For developmental stages, 28S/RPL7 were the optimal reference genes, both $RPL7/EF1{\alpha}$ were suitable for experiments of different tissues, whereas for insecticide treatments, $28S/{\alpha}-TUB$ were suitable for normalizations of expression data. In addition, $28S/{\alpha}-TUB$ were the suitable reference genes because they have the most stable expression among different developmental stages, tissues and insecticide treatments. Our work is the first report on reference gene selection in M. separata, and might serve as a precedent for future gene expression studies.

• 정보보호학회논문지
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• 제31권3호
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• pp.323-330
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• 2021

최근에는 양자 컴퓨터의 빠른 연산의 장점이 알려지면서 큐비트를 활용한 양자회로에 대한 관심이 높아지고 있다. 그루버 알고리즘은 n-bit의 보안 레벨의 대칭키 암호와 해시 함수를 n/2-bit 보안 레벨까지 낮출 수 있는 양자 알고리즘이다. 그루버 알고리즘은 양자 컴퓨터상에서 동작하기 때문에 적용 대상이 되는 대칭키 암호와 해시함수는 양자 회로로 구현되어야 한다. 이러한 연구 동기로, 최근 들어 대칭키 암호 또는 해시 함수를 양자 회로로 구현하는 연구들이 활발히 수행되고 있다. 하지만 현재는 큐비트의 수가 제한적인 상황으로 최소한의 큐비트 개수로 구현하는 것에 관심을 가지고 효율적인 구현을 목표로 하고 있다. 본 논문에서는 국산 해시함수 LSH 구현에 큐빗 재활용, 사전 연산을 통해 사용 큐빗 수를 줄였다. 또한, Mix, Final 함수와 같은 핵심 연산들을 IBM에서 제공하는 양자 프로그래밍 툴인 ProjectQ를 사용하여 양자회로로 효율적으로 구현하였고 이에 필요한 양자 자원들을 평가하였다.

• 정보보호학회논문지
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• 제31권6호
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• pp.1149-1156
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• 2021

Brute force 공격을 가속화 시키는 Grover 알고리즘은 대칭키 암호의 키 복구에 적용 가능하며, NIST에서는 대칭키 암호에 대한 Grover 공격 비용을 양자 후 보안 강도 추정에 활용하고 있다. 본 논문에서는 DES를 양자 회로로 최적화 구현하여 Grover 알고리즘 공격 비용을 추정한다. NIST에서는 128, 192, 256-bit 키를 사용하는 대칭키 암호에 대해 AES의 공격 비용을 기준으로 양자 후 보안 강도를 추정하고 있다. DES에 대해 추정한 공격 비용은 DES가 양자 컴퓨터의 공격으로부터 어느 정도의 내성을 가지고 있는지 분석해볼 수 있다. 현재 64-bit 키를 사용하는 대칭키 암호들에 대한 양자 후 보안 지표가 아직 존재하지 않기 때문에 본 논문에서 추정한 64-bit 키를 사용하는 DES에 대한 Grover 공격 비용이 기준으로 활용될 수 있다. 제안하는 DES의 양자 회로 구현 적합성 및 공격 비용 분석에는 양자 프로그래밍 툴인 ProjectQ가 사용되었다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2023년도 학술발표회
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• pp.156-156
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• 2023

본 연구는 SWAT(Soil and Water Assessment Tool)과 PHABSIM(Physical Habitat Simulation System)을 활용하여 남강댐유역(2,983.0 km²)을 대상으로 유역환경변화에 따른 환경생태유량 확보량을 산정하였다. 유역환경변화를 고려하기 위해 유역환경변화 요인(토지이용, 지하수 이용, 산림생장, 도로개발, 토양깊이)를 1980s(1976~1985), 2010s(2006~2019)로 구분하여 보정된 SWAT에 적용하였다. 유역환경변화 분석결과 토지이용은 1980s 대비 2010s에서 도시와 농업지역은 증가하였으나, 산림과 수역은 감소하였다. 지하수 이용은 1980s 대비 2010s에서 +18.9 백만 m³/년 증가한 평균 31.5 백만 m³/년으로 분석되었고, 산림높이는 1980s 대비 2010s에서 +0.6 m 증가한 평균 12.4 m의 수고를 가지는 것으로 분석되었다. 토양깊이와 도로망의 경우 각각 1980s 대비 2010s에서 -0.2 cm, +29.2 km 증가한 61.3 cm, 51.5 km로 나타났다. 유역환경변화 요인을 SWAT에 적용한 결과, 남강댐유역의 평균 유량은 1980s 대비 2010s에서 -9.5 m³/sec 감소한 77.3 m³/sec로 분석되었다. 남강댐유역의 환경생태유량을 산정하기 위해 하류에 위치한 정암교가 위치한 하천에 대해 PHABSIM을 구축하였고, 대표어종인 피라미에 대한 서식처적합도지수를 적용하여 환경생태유량을 산정하였다. 최적 환경생태유량은 21.0 m³/sec로 나타났고, 가중가용면적-유량 관계를 활용하여 가중가용면적 비율별(100%~25%) 환경생태유량을 산정하였다. 2010s에서 환경생태유량을 만족하지 못하는 73일(Q293~Q365)에 대하여 각 유황과 환경생태유량과의 차이를 일별로 계산한 후 10일 간격의 차이의 총합을 확보량으로 정의하여 산정하였다. 100% 환경생태유량 기준일 때 평균 확보량과 확보기간은 각각 5.36 m³/sec, 73일로 나타났고, 80% 기준일 때 평균 확보량과 확보기간은 각각 2.75 m³/sec, 20일로 나타났다.

• 한국동물위생학회지
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• 제45권1호
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• pp.19-30
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• 2022

In this study, a simple loop-mediated isothermal amplification (LAMP) combined with visual detection method (vLAMP) assay was developed for the rapid and specific detection of African swine fever virus (ASFV), overcoming the shortcomings of previously described LAMP assays that require additional detection steps or pose a cross-contamination risk. The assay results can be directly detected by the naked eye using hydroxynaphthol blue after incubation for 40 min at 62℃. The assay specifically amplified ASFV DNA and no other viral nucleic acids. The limit of detection of the assay was <50 DNA copies/reaction, which was ten times more sensitive than conventional polymerase chain reaction (cPCR) and comparable to real-time PCR (qPCR). For clinical evaluation, the ASFV detection rate of vLAMP was higher than cPCR and comparable to OIE-recommended qPCR, showing 100% concordance, with a κ value (95% confidence interval) of 1 (1.00~1.00). Considering the advantages of high sensitivity and specificity, no possibility for cross-contamination, and being able to be used as low-cost equipment, the developed vLAMP assay will be a valuable tool for detecting ASFV from clinical samples, even in resource-limited laboratories.