• Analytical Science and Technology
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• v.30 no.1
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• pp.10-19
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• 2017

Anabolic steroids have similar structures to testosterone, both of which promote the growth of muscle mass and increase strength. However, the side effects of anabolic steroid use may lead to heart attacks or strokes. Additionally, the excessive use of steroids inhibits the production of the sex hormones in the body via a negative feedback loop, which results in testicular atrophy in males and amenorrhea in females. Currently, the method of choice used to test for the presence of anabolic steroids is GC-MS. However, GC-MS methods require chemical derivatization of the steroid sample to ensure compatibility with the analytical method; therefore, analysis of many different samples is difficult and time consuming. Unlike GC-MS, the liquid chromatography-quadrupole-time of flight mass spectrometry (LC-Q-TOF-MS) method is suitable for many samples. Twenty-two different anabolic steroids were analyzed by LC-Q-TOF-MS with various collision energies (CE). Accurate mass spectral data were obtained using a Q-TOF-MS equipped with an electro-spray ionization source and operated in the positive MS/MS mode for several classes of steroids that are often the targets of testing. Based on the collected data, fragmentation pathways were carefully elucidated. The high selectivity and sensitivity of the LC-Q-TOF-MS instrument combined with these fragmentation pathways offers a new approach for the rapid and accurate screening of anabolic steroids. The obtained data from the 22 different anabolic steroids will be shared with the scientific community in order to establish a library to aid in the screening of illegal anabolic steroids.

• Journal of Microbiology and Biotechnology
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• v.31 no.3
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• pp.358-367
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• 2021

The World Health Organization (WHO) has declared the coronavirus disease 2019 (COVID-19) as an international health emergency. Current diagnostic tests are based on the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method, which is the gold standard test that involves the amplification of viral RNA. However, the RT-qPCR assay has limitations in terms of sensitivity and quantification. In this study, we tested both qPCR and droplet digital PCR (ddPCR) to detect low amounts of viral RNA. The cycle threshold (CT) of the viral RNA by RT-PCR significantly varied according to the sequences of the primer and probe sets with in vitro transcript (IVT) RNA or viral RNA as templates, whereas the copy number of the viral RNA by ddPCR was effectively quantified with IVT RNA, cultured viral RNA, and RNA from clinical samples. Furthermore, the clinical samples were assayed via both methods, and the sensitivity of the ddPCR was determined to be equal to or more than that of the RT-qPCR. However, the ddPCR assay is more suitable for determining the copy number of reference materials. These findings suggest that the qPCR assay with the ddPCR defined reference materials could be used as a highly sensitive and compatible diagnostic method for viral RNA detection.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.46 no.4
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• pp.148-156
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• 2009

This paper presents a new image rejection algorithm based on the analysis of the distortion of a single-frequency continuous-wave (CW) signal due to the I/Q mismatch. Existing methods estimated the gain mismatch and phase mismatch on RF receivers and compensated them However, this paper shows that the circular trajectory of a single-frequency CW signal is distorted elliptic-type trajectory due to the I/Q mismatch. Utilizing the analysis, we propose a I/Q mismatch compensation method. It has two processing steps. In the first processing step, the generated signal is rotated to align the major axis of the elliptic-type trajectory diagram with the x-axis. In the second processing step, the Q-channel signal in the regenerated signal is scaled to align the regenerated signal with the transmitted single-frequency CW signal. Simulation results show that a receiver using the proposed image rejection algorithm can achieve an image rejection ratio of more than 70dB. And, simulation results show that the bit error rate performances of receivers using the proposed image rejection algorithm are almost the same as those of conventional coherent demodulators, even in fading channels.

• Korean Journal of Adult Nursing
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• v.17 no.2
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• pp.259-267
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• 2005

Purpose: The purpose of the study was to investigate change of life in middle-aged men. Method: The research method employed Q-methodology. twenty-five participants rated 39 selected Q-statements on a scale of 1~9. The collected data were analyzed using pc-QUNAL software. Result: Principal component analysis identified 3 types of change of life in middle-aged men. The categories were labeled 'Mature-oriented', 'Effeminate-oriented', 'Juvenescence-oriented'. Conclusion: We have found change of life in middle-aged men through this research. To setup and apply differences based on this result is needed.

• The Korean Journal of Nuclear Medicine Technology
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• v.22 no.1
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• pp.90-97
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• 2018

Purpose The lung segment ratio which is obtained through quantitative analyses of lung perfusion scan images is calculated to evaluate the lung function pre and post surgery. In this Study, the planar image production methods by using Q-Metrix (GE Healthcare, USA) program capable of not only quantitative analysis but also computation of the segment ratio after having performed SPECT/CT are comparatively evaluated. Materials and Methods Lung perfusion scan and SPECT/CT were performed on 50 lung cancer patients prior to surgery who visited our hospital from May 1, 2015 to September 13, 2016 by using Discovery 670(GE Healthcare, USA) equipment. AP(Anterior Posterior)method that uses planar image divided the frontal and rear images into three rectangular portions by means of ROI tool while PO(Posterior Oblique)method computed the segment ratio by dividing the right lobe into three parts and the left lobe into two parts on the oblique image. Segment ratio was computed by setting the ROI and VOI in the CT image by using Q-Metrix program and statistically analysis was performed with SPSS Ver. 23. Results Regarding the correlation concordance rate of Q-Metrix and AP methods, RUL(Right upper lobe), RML(Right middle lobe) and RLL(Right lower lobe) were 0.224, 0.035 and 0.447. LUL(Left upper lobe) and LLL(Left lower lobe) were found to be 0.643 and 0.456, respectively. In the PO method, the right lobe were 0.663, 0.623 and 0.702, respectively, while the left lobe were 0.754 and 0.823. When comparison was made by using the Paired sample T-test, Right lobe were $11.6{\pm}4.5$, $26.9{\pm}6.2$ and $17.8{\pm}4.2$, respectively in the AP method. Left lobe were $28.4{\pm}4.8$ and $15.4{\pm}5.6$. The right lobe of PO had values of $17.4{\pm}5.0$, $10.5{\pm}3.6$ and $27.3{\pm}6.0$, while the left lobe had values of $21.6{\pm}4.8$ and $23.1{\pm}6.6$, thereby having statistically significant difference in comparison to the Q-Metrix method for each of the lobes (P<0.05). However, there was no statistically significant difference in Right middle lobe (P>0.05). Conclusion The AP method showed low concordance rate in correlation with the Q-Metrix method. However, PO method displayed high concordance rate overall. although AP method had significant differences in all lobes, there was no significant difference in Right middle lobe of PO method. Therefore, at the time of production of lung perfusion scan results, utilization of Q-Metrix method of SPECT/CT would be useful in computation of accurate resultant values. Moreover, it is deemed possible to expect obtain more practical sectional computation result values by using PO method at the time of planar image acquisition.

• Journal of Korean Tunnelling and Underground Space Association
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• v.5 no.2
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• pp.209-216
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• 2003

The present with in this country, rock bolt installation, shotcrete, and concrete lining in construction order has become accepted as a general tunnelling method in NATM. On the other hand Unlined tunnelling method, which was developed by many countries near Scandinavian Peninsula with hard rocks comparatively, has recently been introduced all over the world, and numerous studies about that have been being devoted to domestic tunnels. Unlined tunnelling method has been developed on the basis of the permanently unsupported openings, and general 7 requirements for them were suggested by Nick Barton. There are no case record about these conditions for Q-system in this country. Therefore, input parameters for Q-system under these conditions were applied to general road tunnel cross-section and numerical analyses for each condition were executed with UDEC-BB, Distinct Element Method.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.9
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• pp.3723-3727
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• 2015

The p53 tumor suppressor protein is a principal mediator of growth arrest, senescence, and apoptosis in response to a broad array of cellular damage. p53 is a substrate for the ubiquitin-proteasome system, however, the ubiquitin-conjugating enzymes (E2s) involved in p53 ubiquitination have not been well studied. UBE2Q1 is a novel E2 ubiquitin conjugating enzyme gene. Here, we investigated the effect of UBE2Q1 overexpression on the level of p53 in the MDA-MB-468 breast cancer cell line as well as the interaction between UBE2Q1 and p53. By using a lipofection method, the p53 mutated breast cancer cell line, MDA-MB-468, was transfected with the vector pCMV6-AN-GFP, containing UBE2Q1 ORF. Western blot analysis was employed to verify the overexpression of UBE2Q1 in MDA-MB-468 cells and to evaluate the expression level of p53 before and after cell transfection. Immunoprecipitation and GST pull-down protocols were used to investigate the binding of UBE2Q1 to p53. We established MDA-MB-468 cells that transiently expressed a GFP fusion proteins containing UBE2Q1 (GFP-UBE2Q1). Western blot analysis revealed that levels of p53 were markedly lower in UBE2Q1 transfected MDA-MB-468 cells as compared with control MDA-MB-468 cells. Both in vivo and in vitro data showed that UBE2Q1 co-precipitated with p53 protein. Our data for the first time showed that overexpression of UBE2Q1can lead to the repression of p53 in MDA-MB-468 cells. This repression of p53 may be due to its UBE2Q1 mediated ubiquitination and subsequent proteasome degradation, a process that may involve direct interaction of UBE2Q1with p53.

• 한국정보통신설비학회:학술대회논문집
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• 2007.08a
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• pp.436-439
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• 2007

This paper describes the optimization of PGS(Patterned Ground Shield) of 5.5 turns rectangular spiral inductor using Taguchi's method. PGS is decrease method of parasite component by silicon substrate among dielectric loss reduction method. By using the taguchi's method, each parameter is fixed upon that PGS high poison(A), slot spacing(B), strip width(C) and overlap turn number(D) of PGS design parameter. Then we verified that percentage contribution and design sensitivity analysis of each parameter and level by signal to noise ratio of larger-the-better type. We consider percentage contribution and design sensitivity of each parameter and level, and then verify that model of optimization for PGS is lower inductance decreasing ratio and higher Q-factor increasing ratio by EM simulation.

• Child Health Nursing Research
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• v.5 no.1
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• pp.5-17
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• 1999

This study was designed to determine the schemata and their characteristics of stress experience the subjectivity of stress experience(structure of subjectivity) would be a basic step for the effective clinical education through the stress management for characteristics of these types. Q-methodological method was used for that purpose. The research method statements were collected prior to the study through indepth interviews. For the study, 31 Q-statements were selected. There were 34 nursing students as subjects for the research. The 34 nursing students sorted the 31 statements using the principal of forced normal distribution. The principle of forced normal distribution, which has 9 scales to measure the individual opinions, was called. Q-factor analysis by using PC QUANL program supply the material. According to the outcomes of this study, there were 3 types of special opinion about the stress experience of clinical practice. The first type is called 'Influence of practical atmosphere type'. Members of this type experienced stress by an inadequate orientation and undesirable role model of nursing. The second type is called 'conflict of nursing role type'. Members of this type experienced stress by an inadequate orientation and undesirable role model of nursing. The third type is called 'Lack of confidence type'. Members of this type experienced stress because of a lack of confidence for their own nursing knowledge and skill. As a result, we now need further study to identify individual psychological aspects of stress for clinical practice. The findings will guide the development in effective approaches for clinical education. Finally, the result of the study will provide us the need for developing systematic and integrated practice education program for students and active involvement of clinical instructor.

• Journal of the Korean Geotechnical Society
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• v.28 no.1
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• pp.29-39
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• 2012

The maximum unit point resistance ($q_{max}$) of rock socketed drilled shafts subjected to axial loads was investigated by a numerical analysis. A 3D Finite Difference Method (FDM) analysis and a Distinct Element Method (DEM) analysis were performed with varying rock elastic modulus (E), discontinuity spacing ($S_j$), discontinuity dip angle ($i_j$), and pile diameter (D). Based on the results of obtained, it was found that the ultimate point resistance ($q_{max}$) increased as rock elastic modulus (E) and rock discontinuity spacing ($S_j$) increased. But, it was found that $q_{max}$ decreased as pile diameter (D) increased. As for the influence of the dip angle of rock discontinuity ($i_j$), it was shown that $q_{max}$ decreased up to 50% of maximum value within the range of $0^{\circ}$ < $i_j$ < $60^{\circ}$ due to the shear failure at rock discontinuities. Furthermore, it was found that if $20^{\circ}{\leq}i_j{\leq}40^{\circ}$, influence of $i_j$ should be taken into account because $q_{max}$ tended to approach a minimum value as $i_j$ approached a value near the friction angle of the discontinuity (${\phi}_j$).