• 환경생물
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• 제32권2호
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• pp.95-101
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• 2014

한반도의 낙엽성 참나무인 졸참나무(Quercus serrata)의 생태적 지위폭이 $CO_2$ 농도와 온도가 상승하였을 때 광, 수분 그리고 영양소 구배에서 어떻게 변화하는지 알아보고자 유리온실에서 대조구와 온난화처리구로 구분하여 재배한 후, 유식물의 20가지 형질을 측정하고 생태적 지위폭을 계산하였다. 그 결과, 생태적 지위폭은 광구배에서 대조구보다 온난화처리구가 넓어졌고, 수분과 영양소 구배에서는 대조구보다 온난화처리구가 좁아졌다. 이것은 지구온난화 진행 시 졸참나무는 광 환경에 대한 내성이 증가되고, 수분과 영양소 환경에 대한 내성이 감소될 것으로 예측할 수 있다. 주성분분석(PCA) 결과, 각 환경 구배에서 요인 1과 요인 2에 의해 대조구와 온난화처리구가 구분되었다. 생태학적 반응은 다양한 형질이 종합적으로 관여하며, 그 중에서 식물의 생산량을 나타내는 형질이 많은 부분 관여하는 것으로 판단된다.

• 동의생리병리학회지
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• 제16권3호
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• pp.499-506
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• 2002

Two of the essential processes required for metastasis are neoangiogenesis and tumor cell invasion of basement membranes (BM) and extracellular matrix (ECM). Recently, data showed that herbs removing blood stasis has an anti-angiogenic effects. Tonifying vital Qi and eliminating pathogenic factor was a basic modality in Oriental oncology. In this study, we investigated several Qi and Blood tonics for potent angiogenic inhibitors. Methanol extracts of samples inhibited the proliferation of ECV-304 at the concentration of 100 ㎍/㎖. Zizyphi Fructus, Glycyrrhizae Radix, Angelicae Gigantis Radix decreased the gelatinolytic activity of MMP-9 from ECV-3Q4, at the concentration of 100 ㎍/㎖ in gelatin zymography. In in vitro invasion assay, herbs inhibited the invasion activity of ECV-304 by 53% of control (Ginseng Radix), 39% (Zizyphi Fructus), 36% (Angelicae Gigantis Radix), 25% (Glycyrrhizae Radix). Ginseng Radix inhibited the capillary-like tube formation of ECV-304 at the concentration of 160 ㎍/㎖, Angelicae Gigantis Radix and Paeoniae Radix Alba inhibited at the concentration of 320 ㎍/㎖. These results indicated that Ginseng Radix, Glycyrrhizae Radix, and Angelicae Gigantis Radix could be considered as potent angiogenic inhibitiors.

• 생명과학회지
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• 제17권4호
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• pp.581-586
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• 2007

본 연구에서는 피부상재균이며 기회병원균이기도 한 Staphylococcus epidermidis ATCC12228로부터 urease효소를 4단계 크로마토그라피 방법을 사용하여 1,127배 정제하고 그 생화학적인 특성을 규명하였다. 정제된 urease 효소는 SDS-PACE 전기영동분석 및 gel-filtration 크로마토그라피를 이용한 천연분자량 분석결과, 67, 16.1 및 12.7 kDa의 3개 subunit가 3량체로 회합되어 존재하는 것으로 나타났으며 catalytic unit 당 2.2개의 니켈 원소를 함유하는 것으로 측정되었다. 정제된 효소의 비활성은 993.8 U/mg, $K_m$값은 8.5mM로 각각 산출되었다.

• Biomolecules & Therapeutics
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• 제24권1호
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• pp.19-24
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• 2016

Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide. The exploration of new biomarkers with high sensitivity and specificity for early diagnosis of AMI therefore becomes one of the primary task. In the current study, we aim to detect whether there is any heart specific long noncoding RNA (lncRNA) releasing into the circulation during AMI, and explore its function in the neonatal rat cardiac myocytes injury induced by $H_2O_2$. Our results revealed that the cardiac-specific lncRNA MHRT (Myosin Heavy Chain Associated RNA Transcripts) was significantly elevated in the blood from AMI patients compared with the healthy control ($^*p<0.05$). Using an in vitro neonatal rat cardiac myocytes injury model, we demonstrated that lncRNA MHRT was upregulated in the cardiac myocytes after treatment with hydrogen peroxide ($H_2O_2$) via real-time RT-PCR (qRT-PCR). Furthermore, we knockdowned the MHRT gene by siRNA to confirm its roles in the $H_2O_2$-induced cardiac cell apoptosis, and found that knockdown of MHRT led to significant more apoptotic cells than the non-target control ($^{**}p<0.01$), indicating that the lncRNA MHRT is a protective factor for cardiomyocyte and the plasma concentration of MHRT may serve as a biomarker for myocardial infarction diagnosis in humans AMI.

• Molecular & Cellular Toxicology
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• 제4권4호
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• pp.307-310
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• 2008

AAD16034 is an alginate lyase from Pseudoalteromonas sp. IAM14594. A very close homologue with known 3D structure exists (marine bacterium Pseudoalteromonas sp. strain no. 272). A three-dimensional structure of AAD16034 was generated based on this template (PDB code: 1J1T) by comparative modeling. The modeled enzyme exhibited a jelly-roll like structure very similar to its template structure. Both enzymes possess the characteristic alginate sequence YFKhG+Y-Q. Since AAD16034 displays enzymatic activity for poly-M alginate, docking of a tri-mannuronate into the modeled structure was performed. Two separate and adjacent binding sites were found. The ligand was accommodated inside each binding site. By considering both binding sites, a plausible binding pose for the poly-M alginate polymer could be deduced. From the modeled docking pose (i.e., the most important factor that attracts alginate polymer into this lyase) the most likely interaction was electrostatic. In accordance with a previous report, the hydroxyl group of Y345 was positioned close to the ${\alpha}$-hydrogen of ${\beta}$-mannuronate, which was suitable to initiate a ${\beta}$-elimination reaction. K347 was also very near to the carboxylatemoiety of the ligand, which might stabilize the dianion intermediate during the ${\beta}$-elimination reaction. This implies that the characteristic alginate sequence is absolutely crucial for the catalysis. These results may be exploited in the design of novel enzymes with desired properties.

• Reproductive and Developmental Biology
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• 제36권2호
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• pp.121-131
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• 2012

After spermatogenesis, spermatozoa come in contact with fluids in the epididymis where they mature. During ejaculation, spermatozoa are mixed with secretions from prostate gland, vesicular glands, and bulbourethral glands. During natural mating, seminal plasma is deposited in the female reproductive tract eliciting various physiological and immunological responses. With the advances in proteomics, the components of seminal plasma have been identified and the information may be valuable in identifying markers for fertility. Components of seminal plasma that affect fertility have been discovered and the mechanism of action of these factors has been determined. The objective of this study was to determine the specific seminal plasma proteins from Korean native cattle, Hanwoo, and Korean native brindle cattle (KNBC) with the long term goal of improving fertilization rate. After SDS-PAGE and 2-dimensional gel electrophoresis, proteins were identified by Q-ToF analysis. They include plasma serine protease inhibitor precursor and platelet-activating factor acetylhydrolase after SDS-PAGE. Number and density of the spots in 2-dimensional gels were higher in KNBC than Hanwoo. Proteins identified from the paired spots of both breeds include chain A, bull seminal plasma PDC-109 Fibronectin Type II module, BSP-30 kDa precursor, and Spermadhesin Z13 or its precursor. Interestingly, some proteins were identified from multiple spots. The functional differences of these diverse forms of the proteins may require further studies. With their previously reported roles in sperm capacitation by these proteins, the studies on the mechanism of action, ligand interaction and the variation in the genome may help improving fertility in cattle.

• 한국세라믹학회지
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• 제54권2호
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• pp.150-157
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• 2017

Three types of piezoelectric single crystals [PMN-PT (Generation I $[Pb(Mg_{1/3}Nb_{2/3})O_3-PbTiO_3]$), PMN-PZT (Generation II $[Pb(Mg_{1/3}Nb_{2/3})O_3-Pb(Zr,Ti)O_3]$), PMN-PZT-Mn (Generation III)] were grown by the solid-state single crystal growth (SSCG) method, and their dielectric and piezoelectric properties were measured and compared. Compared to (001) PMN-PT and PMN-PZT single crystals, the (001) PMN-PZT-Mn single crystals exhibited a higher transition temperature between the rhombohedral and tetragonal phases ($T_{RT}=144^{\circ}C$), as well as a higher coercive electric field ($E_C=6.3kV/cm$) and internal bias field ($E_I=1.6kV/cm$). The (011) PMN-PZT-Mn single crystals showed the highest coercive electric field ($E_C=7.0kV/cm$), and the highest stability of $E_C$ and $E_I$ during 60 cycles of polarization measurement. These results demonstrate that both Mn doping (for higher electromechanical quality factor ($Q_m$)) and a (011) crystallographic orientation (for higher coercive electric field and stability) are necessary for high power transducer applications of these piezoelectric single crystals. Specifically, the (011) PMN-PZT-Mn single crystal (Gen. III) had the highest potential for application in the fields of SONAR transducers, high intensity focused ultrasound (HIFU), ultrasonic motors, and others.

• 한국전자파학회논문지
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• 제23권4호
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• pp.515-523
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• 2012

본 논문에서는 구형 유전체 위에 있는 구형 패치의 전파 특성으로서 공진 특성과 방사 특성을 분석하였다. 공진 주파수와 Q를 계산하여 특정 모드의 공진 특성을 해석하였으며, 원거리에서의 전기장을 계산하여 방사 특성을 해석하였다. 구형 유전체의 해석을 위해 스펙트럴 영역 해석법(spectral domain analysis method)를 적용하였으며, Vector Legendre transform pair와 Galerkin's method를 이용하여 스펙트럴 영역으로 변환한 후 대수식으로 정리하여 효율적인 계산을 할 수 있었으며, 이를 통해 구형 패치의 반지름이나 곡률, 유전체의 특성이 구형 패치의 특성에 미치는 영향을 해석하였다.

• 한국자기학회지
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• 제6권6호
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• pp.367-374
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• 1996

자기소자중 가장 기본이 되는 박막인덕터의 제작에 관한 연구를 수행하기 위하여, photolitho-graphy와 에칭공정을 도입하고, 도체간격 및 도체폭이 수십 $\mu\textrm{m}$, 도체코일 턴수가 각각 13회와 20회, 크기가 $4\;mm{\times}4\;mm$인 공심형 박막인덕터를 제작하였다. 이것을 마이크로스트립선로에 정착하고, network analyzer로 주파수 1 MHz ~ 1 GHz에서 신호의 반사계수법을 이용하여 간편하고 비교적 정확한 측정을 하였다. 특히, 공정이 간단한 습식 에칭공정을 도입하여, 안정된 에칭기술을 통해 양호한 미세패턴구조를 얻었다. 박막인덕터의 특성은, 크기가 같을때, L 및 Q값은 spiral형이 meander형 보다 큰 값을 갖는 반면, 공진주파수는 인덕턴스의 증가에 의한 영향으로 spiral형이 meander형보다 감소하였다.

• 한국광학회지
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• 제10권2호
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• pp.162-168
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• 1999

본 연구에서는 개념적으로 기생 발진을 비롯한 증폭기의 오동작 가능성을 최소화하고 좁은 선폭의 연속파 레이저를 펄스 증폭하기 위한 목적으로 새로운 구조를 갖는 사중경로 색소 레이저 증폭기를 제안하고 실험적으로 구현하였다. 펌핑 레이저의 펄스 에너지가 5.6 mJ이고 연속파 레이저의 입력 강도 100 mW일 때, 사중경로 증폭기는 약 130 MHz(FWHM)의 선폭과 1.5 mJ의 에너지를 갖는 레이저 펄스를 출력하였으며, 이는 약 2$\times$106 이상의 높은 증폭 이득과 27%의 에너지 효율에 해당하는 것이다. 사중경로 증폭기 내에 회절격자를 사용하면, 파장 선택 소자가 없는 보통의 증폭기와 비교할 때 총 출력 에너지가 4% 정도 증가됨과 동시에 ASE가 차지하는 비율이 10배 이상 감소하여, 총 출력 빔에 대해 ASE 에너지가 1.5% 이하로 억제된다.