• Journal of Animal Science and Technology
• /
• v.64 no.4
• /
• pp.800-811
• /
• 2022

The integration of metabolomics and transcriptomics may elucidate the correlation between the genotypic and phenotypic patterns in organisms. In equine physiology, various metabolite levels vary during exercise, which may be correlated with a modified gene expression pattern of related genes. Integrated metabolomic and transcriptomic studies in horses have not been conducted to date. The objective of this study was to detect the effect of moderate exercise on the metabolomic and transcriptomic levels in horses. In this study, using nuclear magnetic resonance (NMR) spectroscopy, we analyzed the concentrations of metabolites in muscle and plasma; we also determined the gene expression patterns of branched chain (alpha) keto acid dehydrogenase kinase complex (BCKDK), which encodes the key regulatory enzymes in branched-chain amino acid (BCAA) catabolism, in two breeds of horses, Thoroughbred and Jeju, at different time intervals. The concentrations of metabolites in muscle and plasma were measured by ¹H NMR (nuclear magnetic resonance) spectroscopy, and the relative metabolite levels before and after exercise in the two samples were compared. Subsequently, multivariate data analysis based on the metabolic profiles was performed using orthogonal partial least square discriminant analysis (OPLS-DA), and variable important plots and t-test were used for basic statistical analysis. The stress-induced expression patterns of BCKDK genes in horse muscle-derived cells were examined using quantitative reverse transcription polymerase chain reaction (qPCR) to gain insight into the role of transcript in response to exercise stress. In this study, we found higher concentrations of aspartate, leucine, isoleucine, and lysine in the skeletal muscle of Jeju horses than in Thoroughbred horses. In plasma, compared with Jeju horses, Thoroughbred horses had higher levels of alanine and methionine before exercise; whereas post-exercise, lysine levels were increased. Gene expression analysis revealed a decreased expression level of BCKDK in the post-exercise period in Thoroughbred horses.

• The Korea Journal of Herbology
• /
• v.32 no.2
• /
• pp.77-85
• /
• 2017

Objectives : This study aimed to investigate the unknown mechanisms behind the anti- inflammatory activity of Hyeonto-dan(HT) 70% ethanol extract on LPS-stimulated RAW 264.7 cells. Methods : Cells were treated with Hyeonto-dan 1 h prior to addition of 200 ng/mL of LPS. Cell viability was measured by the MTS assay. Nitric oxide levels were determined by the Griess assay. $PGE_2$ were measured using EIA kit. Pro-inflammatory cytokine production was measured by the enzyme-linked immunosorbent assay (ELISA). The expression of COX-2, iNOS, and MAPKs was investigated by Western blot, qRT-PCR. $NF-{\kappa}B$/p65 localization and interaction of the TLR-4 receptor with LPS was examined by immunofluorescence assays. Results : Hyeonto-dan had no cytotoxicity at the measured concentration. Hyeonto-dan inhibited NO production and pro-inflammatory cytokines such as IL-6, $TNF-{\alpha}$, and PGE2 as well as the protein and mRNA expression of iNOS and COX-2. Moreover, Hyeonto-dan inhibited the interaction between LPS and TLR-4 in murine macrophages. It suppressed phosphorylation of extracellular signal-regulated kinase (ERK 1/2), c-jun N-terminal kinase (JNK 1/2) and p38. Finally, it inhibited translocation of $NF-{\kappa}B$ in response to competitive LPS. Conclusions : Based on the results of this study, Hyeonto-dan inhibited the binding of TLR-4 receptor to LPS and inhibited the phosphorylation of extracellular signaling pathway MAPKs. These inhibitory effects are thought that the amount of $NF-{\kappa}B$ delivered to the nucleus was decreased and the inflammatory reaction was prevented by decreasing the production of LPS-induced $PGE_2$, NO, IL-6 and $TNF-{\alpha}$.

• Journal of Marine Life Science
• /
• v.6 no.2
• /
• pp.73-79
• /
• 2021

Bisphenol A is a representative endocrine disruptor and continuously detected in aquatic environment due to wide use, resulting in adverse effects on growth, development, and reproduction in diverse organisms as well as human. Structural analogs have been developed to substitute BPA are also suspected to have endocrine disrupting effects. In the present study, the time-dependent expression patterns of ecdysteroid synthesis (nvd, cyp314a1), receptors (EcRA, EcRB, USP, ERR), and downstream signaling pathway - related genes (HR3, E75, Vtg, VtgR) were investigated using quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) in the brackish water flea Diaphanosoma celebensis exposed to Bisphenol analogs (BPs; BPA, BPF, and BPS) for 6, 12, and 24 h. As results, the expression of nvd, cyp314a1, EcRs, USP, ERR and E75 mRNA was upregulated at 6 h exposure to BPF, which is earlier than BPA and BPS (12 h). On the other hand, HR3, E75 and VtgR mRNA levels were elevated at 6 h earlier at BPS and BPF than at BPA (12 h), but Vtg mRNA level was slightly changed within 24 h. These findings suggest that like BPA, BPF and BPS can also modulate the transcription of ecdysteroid pathway - related genes with different mechanisms, and have a potential as endocrine disruptors. This study will provide a better understanding the molecular mode of action of bisphenols on ecdysteroid pathway in the brackish water flea.

• Journal of Korean Society of Forest Science
• /
• v.111 no.2
• /
• pp.234-241
• /
• 2022

We performed this study to determine the foliar nutrient concentration and the nutrient stocks of restoration areas and adjacent Pinus densiflora S. et. Z. (red pine) stands in opencast kaolinite mines in Sancheong-gun, Gyeongsangnam-do, southern Korea. We chose six sites to determine foliage nutrient concentrations and the nutrient stocks of soils (0-10 cm depth). The dominant vegetation planted in restoration areas comprised Quercus acutissima Carruth., P. koraiensis S. et. Z., Festuca arundinacea Schreb., and Lespedeza cuneata G. Don. Invading vegetation in the restoration areas comprised Alnus incana (L.) Medik., Robinia pseudoacacia L., and Lespedeza spp., among others. The carbon and nitrogen stocks at 10 cm soil depth were significantly higher in the red pine stands than those in the restoration areas, whereas those of phosphorus, potassium, and magnesium were not significantly different between the two areas. However, calcium stocks were significantly higher in the restoration areas than in the red pine stands. Nitrogen concentration in foliage was higher in L. cuneata (20.28 mg N g^-1) than that in F. arundinacea (5.67 mg N g^-1), whereas potassium concentration was twice as high in F. arundinacea (18.8 mg K g^-1) as that in L. cuneata (9.07 mg K g^-1). Foliar nitrogen concentrations in invasive vegetation such as A. incana, R. pseudoacacia, and Lespedeza spp. were twice or four times higher than those of Q. acutissima and P. koraiensis. Our results indicate the development of suitable vegetation and soil amendment treatments to improve poor soil environmental conditions in restoration areas are necessary following opencast kaolinite mining.

• BMB Reports
• /
• v.55 no.6
• /
• pp.275-280
• /
• 2022

The treatment of atopic dermatitis (AD) is challenging due to its complex etiology. From epidermal disruption to chronic inflammation, various cells and inflammatory pathways contribute to the progression of AD. As with immunosuppressants, general inhibition of inflammatory pathways can be effective, but this approach is not suitable for long-term treatment due to its side effects. This study aimed to identify a plant extract (PE) with anti-inflammatory effects on multiple cell types involved in AD development and provide relevant mechanistic evidence. Degranulation was measured in RBL-2H3 cells to screen 30 PEs native to South Korea. To investigate the anti-inflammatory effects of Parasenecio auriculatus var. matsumurana Nakai extract (PAE) in AD, production of cytokines and nitric oxide, activation status of FcεRI and TLR4 signaling, cell-cell junction, and cell viability were evaluated using qRT-PCR, western blotting, confocal microscopy, Griess system, and an MTT assay in RBL-2H3, HEK293, RAW264.7, and HaCaT cells. For in vivo experiments, a DNCBinduced AD mouse model was constructed, and hematoxylin and eosin, periodic acid-Schiff, toluidine blue, and F4/80-staining were performed. The chemical constituents of PAE were analyzed by HPLC-MS. By measuring the anti-degranulation effects of 30 PEs in RBL-2H3 cells, we found that Paeonia lactiflora Pall., PA, and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. show an inhibitory activity of more than 50%. Of these, PAE most dramatically and consistently suppressed cytokine expression, including IL-4, IL-9, IL-13, and TNF-α. PAE potently inhibited FcεRI signaling, which mechanistically supports its basophil-stabilizing effects, and PAE downregulated cytokines and NO production in macrophages via perturbation of toll-like receptor signaling. Moreover, PAE suppressed cytokine production in keratinocytes and upregulated the expression of tight junction molecules ZO-1 and occludin. In a DNCB-induced AD mouse model, the topical application of PAE significantly improved atopic index scores, immune cell infiltration, cytokine expression, abnormal activation of signaling molecules in FcεRI and TLR signaling, and damaged skin structure compared with dexamethasone. The anti-inflammatory effect of PAE was mainly due to integerrimine. Our findings suggest that PAE could potently inhibit multi-inflammatory cells involved in AD development, synergistically block the propagation of inflammatory responses, and thus alleviate AD symptoms.

• Korean Journal of Environment and Ecology
• /
• v.36 no.1
• /
• pp.87-101
• /
• 2022

This study analyzed changes in the vegetation structure of 16 permanent plots (experimental and control) installed in Wando Arboretum in 1996 to monitor the long-term change process of evergreen broad-leaved forests in warm temperate. Especially, this study discusses the effects of trial treatment (selection cutting and plant introduction), introduced as a restoration technique in 1996, on vegetation development. In communities dominated by Quercus acuta in the canopy (permanent plots 1 through 3), this species's mean important percentage (MIP) decreased, and the evergreen broad-leaved species was introduced from outside increased, likely to change the vegetation structure in the future. The expansion of the evergreen broad-leaved species group was also confirmed in Q. acuta-deciduous broad-leaved forests (permanent plots 5 and 7) and Pinus densifloraforests (permanent plots 9 and 10). In the experimental plots where thinning was carried out, the zoochory (the dispersal of seeds by birds), Cinnamomum yabunikkei, Neolitsea sericea, Machilus thunbergii, etc., and the expansion of the influence of evergreen broad-leaved species were remarkable, so it is considered to have effectively promoted the vegetation development in warm temperate forests. Although evergreen broad-leaved species were planted in the experimental plot to change vegetation structure, it seems the effect on the change was weak due to the small amount of planting. Compared to other vegetation types, the change in the vegetative structure of the pine forest to an evergreen broad-leaved forest was clear due to the decline of P. densiflora and P. thunbergii.

• Korean Journal of Environment and Ecology
• /
• v.36 no.1
• /
• pp.72-86
• /
• 2022

This study was conducted to identify structural characteristics of the evergreen broad-leaved forests distributed in Hak-dong, Geojae island. For a survey, 52 sites were set up in areas with changes in the vegetation community or location environment where Cinnamomum yabunikkei, Neolitsea sericea, and Machilus thunbergii dominated or appeared in the canopy, sub-canopy, or shrub layer. The community classification with TWINSPAN identified the following communities: N. sericea-C. yabunikkei, C. yabunikkei-Camellia japonica, Ca. japonica, Quercus variabilis-Ca. japonica, Pinus thunbergii-Ca. japonica, Castanopsis sieboldii, P. thunbergii, and Platycarya strobilacea-Mallotus japonicus. Considering the result of the study that succession series of warm-temperate forest reflecting the latent natural vegetation is the transition of conifers and deciduous broad-leaved forest to evergreen broad-leaved forest, the communities predominated by the communities predominated by the communities predominated by P. thunbergii, Q. variabilis, and Pl. strobilacea are likely to transform into the evergreen forest predominated by N. sericea and C. yabunikkei. The sites where C. yabunikkei, N. sericea, and Castanopsis sieboldii are dominant in the canopy and sub-canopy layers are likely to maintain the status quo if there is no artificial disturbance. The relationship between the impact of the environmental factors and the vegetation distribution showed silt among the physical properties of the soil directly or indirectly affected it, which was judged to be due to the fact that it was located on a steep slope. The soil acidity (pH) was 5-5.84, electrical conductivity 0.047-0.139 dS/m, and organic matter content was 3.32-12.06%. Although there were differences by the colony, they were generally low.

• The Korean Journal of Physiology and Pharmacology
• /
• v.26 no.5
• /
• pp.335-345
• /
• 2022

Pyroptosis is an inflammatory form of programmed cell death that is linked with invading intracellular pathogens. Cardiac pyroptosis has a significant role in coronary microembolization (CME), thus causing myocardial injury. Tanshinone IIA (Tan IIA) has powerful cardioprotective effects. Hence, this study aimed to identify the effect of Tan IIA on CME and its underlying mechanism. Forty Sprague-Dawley (SD) rats were randomly grouped into sham, CME, CME + low-dose Tan IIA, and CME + high-dose Tan IIA groups. Except for the sham group, polyethylene microspheres (42 ㎛) were injected to establish the CME model. The Tan-L and Tan-H groups received intraperitoneal Tan IIA for 7 days before CME. After CME, cardiac function, myocardial histopathology, and serum myocardial injury markers were assessed. The expression of pyroptosis-associated molecules and TLR4/MyD88/NF-κB/NLRP3 cascade was evaluated by qRT-PCR, Western blotting, ELISA, and IHC. Relative to the sham group, CME group's cardiac functions were significantly reduced, with a high level of serum myocardial injury markers, and microinfarct area. Also, the levels of caspase-1 p20, GSDMD-N, IL-18, IL-1β, TLR4, MyD88, p-NF-κB p65, NLRP3, and ASC expression were increased. Relative to the CME group, the Tan-H and Tan-L groups had considerably improved cardiac functions, with a considerably low level of serum myocardial injury markers and microinfarct area. Tan IIA can reduce the levels of pyroptosis-associated mRNA and protein, which may be caused by inhibiting TLR4/MyD88/NF-κB/NLRP3 cascade. In conclusion, Tanshinone IIA can suppress cardiomyocyte pyroptosis probably through modulating the TLR4/MyD88/NF-κB/NLRP3 cascade, lowering cardiac dysfunction, and myocardial damage.

• Nutrition Research and Practice
• /
• v.16 no.5
• /
• pp.549-564
• /
• 2022

BACKGROUND/OBJECTIVES: Oxidative stress is caused by an imbalance between harmful free radicals and antioxidants. Long-term oxidative stress can lead to an "exhausted" status of antioxidant defense system triggering development of metabolic syndrome and chronic inflammation. Green perilla (Perilla frutescens) is commonly used in Asian cuisines and traditional medicine in southeast Asia. Green perilla possesses numerous beneficial effects including anti-inflammatory and antioxidant functions. To investigate the potentials of green perilla leaf extract (PE) on oxidative stress, we induced oxidative stress by high-fat diet (HFD) in aging mice. MATERIALS/METHODS: C57BL/6J male mice were fed HFD continuously for 53 weeks. Then, mice were divided into three groups for 12 weeks: a normal diet fed reference group (NDcon), high-fat diet fed group (HDcon), and high-fat diet PE treated group (HDPE, 400 mg/kg of body weight). Biochemical analyses of serum and liver tissues were performed to assess metabolic and inflammatory damage and oxidative status. Hepatic gene expression of oxidative stress and inflammation related enzymes were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: PE improved hepatopathology. PE also improved the lipid profiles and antioxidant enzymes, including hepatic glutathione peroxidase (GPx) and superoxide dismutase (SOD) and catalase (CAT) in serum and liver. Hepatic gene expressions of antioxidant and anti-inflammatory related enzymes, such as SOD-1, CAT, interleukin 4 (IL-4) and nuclear factor erythroid 2-related factor (Nrf2) were significantly enhanced by PE. PE also reduced the levels of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) in the serum and liver; moreover, PE suppressed hepatic gene expression involved in pro-inflammatory response; Cyclooxygenase-2 (COX-2), nitric oxide synthase (NOS), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6). CONCLUSIONS: This research opens opportunities for further investigations of PE as a functional food and possible anti-aging agent due to its attenuative effects against oxidative stress, resulting from HFD and aging in the future.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.25 no.3
• /
• pp.50-58
• /
• 1980

A cytoplasmic-genetic male sterile line(MS) in rapes of which fertility is not restored under any temperature regimes and have the better quaility of and oil cake was developed. A MS line named Mokpo-MS, which is form the cross between Tower and Isuzu and its stamens is degenerated completely by interact~g with cytoplasmic and nuclear genes was selected. This MS line was found as a complete cytoplasmic-genetic male sterile having the quality of oil and oil cake were greatly improved by introducing Zero-erucic and Zero-glucosinolate gene from Tower.