• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 한국축산식품학회 2004년도 정기총회 및 제33차 춘계 학술대회
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• pp.89-119
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• 2004

This study was conducted to isolate lactobacilli having probiotic characteristics to be used as health adjuncts with fermented milk products. Acid tolerant strains were selected in Lactobacilli MRS broth adjusted to pH 4.0 from 80 healthy persons (infants, children and adults). And bile tolerant strains were examined in Lactobacilli MRS broth in which 1.0% bile salt was added. By estimation above characteristics, the strains No. 27, which was isolated from adult feces, was selected and identified as Lactobacillus salivarius subsp. salivarius based on carbohydrate fermentation and 16S rDNA sequencing. It was used as a probiotic strain in fermented milk products. The pH of fermented milk decreased from pH 6.7 to 5.0 and titratable acidity increased from 0.3% to 1.0% by L. salivarius subsp. salivarius (isolation strain 20, 35, and 37), when incubated for 36 h at $37^{\circ}C$. The number of viable cell counts of fermented milk was maximized at this incubation condition. The SDS-PAGE evidenced no significant change of casein but distinct changes of whey protein were observed by isolated L. salivarius subsp. salivarius for titratable acidity being incubated by $0.9{\sim}1.0%$ at $37^{\circ}C$. All of the strains produced 83.43 to 131.96 mM of lactic acid and 5.39 to 26.85 mM of isobutyric acid in fermented products. The in vitro culture experiment was performed to evaluate ability to reduce cholesterol levels and antimicrobial activity in the growth medium. The selected L. salivarius subsp. salivarius reduced $23{\sim}38%$ of cholesterol content in lactobacilli MRS broth during bacterial growth for 24 hours at $37^{\circ}C$. All of the isolated L. salivarius subsp. salivarius had an excellent antibacterial activity with $15{\sim}25$ mm of inhibition zone to E. coli KCTC1039, S. enteritidis KCCM3313, S. typhimurium M-15, and S. typhimurium KCCM40253 when its pH had not been adjusted. Also, all of the isolated L. salivarius subsp. salivarius had partial inhibition zone to E. coli KCTC1039, E. coli KCTC0115 and S. enteritidis KCCM3313 when it had been adjusted to pH 5.7. The selected strains were determined to have resistances of twelve antibiotic. Strains 27 and 35 among the L. salivarius subsp. salivarius showed the highest resistance to the antibiotics. Purified ${\alpha}$-galactosidase was obtained by DEAE-Sephadex A-50 ion exchange chromatography, Mono-Q ion exchange chromatography and HPLC column chromatography from L. salivarius subsp. salivarius 27. The specific activity of the purified enzyme was 8,994 units/mg protein, representing an 17.09 folds purification of the original cell crude extract. The molecular weight of enzyme was identified about 53,000 dalton by 12% SDS-PAGE. Optimal temperature and pH for activity of this enzyme were $40^{\circ}C$ and 7.0 respectively. The enzyme was found to be stable between 25 and $50^{\circ}C$. ${\alpha}$-galactosidase activity was lost rapidly below pH 5.0 and above pH 9.0. This enzyme was liberated galactose from melibiose, raffinose, and stachyose, and also the hydrolysis rate of substrate was compound by HPLC. These results indicated that some of the L. salivarius subsp. salivarius (strain 27 and 35) are considered as effective probiotic strains with a potential for industrial applications, but the further study is needed to establish their use as probiotics in vivo.

• Journal of Korean Society of Forest Science
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• 제96권4호
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• pp.438-447
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• 2007

The purpose of this study was to understand the ecosystem structure and function and soil water changes in Tricholoma matsutake producing pine stands. The investigated stands were pine forest in Sogrisan National Park in Chungbuk-do of Korea. For the purpose we investigated main vegetation, leaf area index(LAI) as ecosystem structural factors and measured photosynthesis, transpiration, xylem water potential, and soil water changes as ecosystem functional factors. Vertical vegetation structure of the site was composed of Pinus densiflora as a overstory species, Quercus mongolica as midstory, Rhododendron mucronulatum, R. schlippenbachii and Fraxinus sieboldiana as understory ones. In the stands LAI was 3.8 during June to September, 2.6 in October and 2.1 during November to April. Photosyntheses of the trees were 6.0 to $7.0{\mu}mol\;CO_2/m^2/s$ in August, and for P. densiflora about $4.0{\mu}mol\;CO_2/m^2/s$ and for Q. mongolica $2.0{\mu}mol\;CO_2/m^2/s$ in mid October. However, R. mucronulatum stopped fixing $CO_2$ and F. sieboldiana shed off the leaves already in mid October. Transpirations were 2.5 to $3.5mmol\;H_2O/m^2/s$ in late August and about $1.0mmol/H_2O/m^2/s$ in mid October. Plant water potentials were -10 to -22 bars for P. densiflora and -5 to -12 bars for the other woody species. The lowest potentials was in late August and highest in late October. Soil water in the stand was closely related to topography. Soil water contents were 7 to 11% at the ridge, 8 to 15% at the hillside and 11 to 19% at the base. Soil temperatures were 0.2 to $0.4^{\circ}C$ higher in T. matustuake colony than noncolony. Mid September soil temperature decreased to $19^{\circ}C$ at which T. matsutake forms primordia. In T. matsutake colony soil moisture was 0.5 to 2.0% lower due to metabolism for consuming water. We suggest that the complicate relationships between ecosystem structure and function in Tricholoma matsutake producing pine stand need to be further investigated.

• Korean Journal of Environment and Ecology
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• 제27권1호
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• pp.113-126
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• 2013

This study was performed to introduce ecological education program as for reporting the current vegetation state within the remaining trees as ecological golf course and to provide basic data. The survey site was S Golf course, which is located in Woonseodong, Incheon city. Its total area was about $3,298,428m^2$, but the relict forest was about $225,143m^2$. Existing landuse, topological structure, the flora, actual vegetation, and plants community structure survey were performed within the relict forest. As result of comparing and analyzing the existing land use, the relict forest was distributed in the forested areas (89.2%) and around the area (10.8%) which had been bare land and SAMMOK earthen ramparts. There were two courses (Ocean and Hanul) with the relict forests. The ocean course was compared of a natural forest, such as Quercus spp. mixed forest, Quercus acutissima forest, Pinus thunbergii forest within the rock fields, and an artificial forest (Ailanthus altissima-Robinia pseudoacacia forest, Robinia pseudoacacia forest) and Quercus acutissima - Elaeagnus umbellata forest. On the Hanul course, Pinus rigida forest and Robinia pseudoacacia forest were the main vegetation, which were artificial forest. It was the contrast aspect of vegetation species in a natural forest, a restoration forest and an artificial forest, which were Q. spp. mixed forest 26~28 species in a natural forest within the vegetation type per investigation area, 3 Pinus thunbergii forest species, and 5~7 artificial forest species on the Hanul course. Based on these vegetation status, the Ocean course was designed into ecological theme spaces named 'Quercus spp.' indigenous forest, 'Pinus thunbergii' restoration forest and ecological story of 'SAMMOK earthen ramparts'. The Hannul course was designed into an artificial forest observation area of 'Robinia pseudoacacia' and 'Pinus rigida' and a fragrance forest area of 'Robinia pseudoacacia'. At the time of the discussion about the introduction of eco-friendly approval system of golf course, it would be estimated that this survey would work as a major material not only raising awareness of the golf course on the ecological environment but also providing programs that can contribute to the community.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• 제18권6호
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• pp.87-104
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• 2023

Recently, concerns are growing over small and medium-sized companies holding out on debt as domestic loan interest rates have risen sharply due to the prolonged impact of COVID-19 and soaring raw material prices. In addition, loans from small and medium-sized enterprises, which are difficult in the business environment, are increasingly being rejected due to high loan interest rates and excessive submission documents and financial statements-oriented screening of loans by the financial sector. Therefore, since it is necessary to discuss ways to promote financing and investment by SMEs, this study intends to suggest ways to promote investment through the use of SMEs' ESG systems. The purpose of this study is to suggest that the use of ESG evaluation indicators used as non-financial indicators helps predict the corporate value of SMEs and the importance of SMEs actively participating in ESG information disclosure. This study suggests the necessity of introducing and practicing ESG by SMEs where financing is important, and aims to analyze as an empirical result that the use of non-financial indicators helps predict corporate value. As a result of the study, the ESG performance and corporate value of SMEs showed a positive (+) relationship. It can be seen that both the grades and corporate value of SMEs by ESG sector have a positive (+) influence relationship. The total ESG rating was confirmed to have a positive effect on corporate value, and it was confirmed that SMEs with higher ESG environment, social, and governance ratings were evaluated higher. According to the research results, it is suggested that SMEs also need to use ESG evaluation indicators, and in order to promote the growth of SMEs, it is suggested that research on ways to re-examine the corporate value of SMEs is necessary. Therefore, this study suggests that the use of ESG should be actively recommended and implemented as a way to establish a management strategy for SMEs, and that efforts to disclose ESG information can soon help SMEs solve information asymmetry. In addition, SMEs want to understand the investment mechanism that the introduction and practice of ESG can lead to the improvement of the value of SMEs and suggest the necessity of SME-type ESG policies in the future.

• Journal of The Korean Society of Inherited Metabolic disease
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• 제17권1호
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• pp.31-37
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• 2017

Carbamoyl phosphate synthetase 1 deficiency (CPS1D) is an autosomal recessive disorder of the urea cycle that causes hyperammonemia. Two forms of CPS1D are recognized: a lethal neonatal type and a less severe, delayed-onset type. Neonatal CPS1D cases often present their symptoms within the first days of life. Delayed-onset cases are predominantly adolescents or adults, and infantile delayed-onset cases are rare. Severe hyperammonemia in the neonatal period leads to serious brain damage, coma, and death if not treated promptly. Therefore, early diagnosis and acute treatment are crucial. Despite the improvement of treatments, including continuous hemodialysis, ammonia-lowering agents, and a low-protein diet, the overall outcome of severe forms of hyperammonemia often remains disappointing. As the liver is the only organ in which ammonia is converted into urea, liver transplantation has been considered as an elegant and radical alternative therapy to classical dietary and medical therapies. However, liver transplantation has many disadvantages, such as a considerable risk for technical complications and perioperative metabolic derangement, especially in neonates. Additionally, there is a lack of suitable donor organs in most countries. According to recent studies, liver cell transplantation is a therapeutic option and serves as a bridge to liver transplantation. Here, we report a Korean CPS1D patient with novel mutations in CPS1 who was treated by liver cell transplantation after being diagnosed in the neonatal period and showed a good neurodevelopmental outcome at the last follow-up at six months of age.

• The Korean Journal of Financial Management
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• 제24권1호
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• pp.29-58
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• 2007

We develop a model to estimate the value of growth opportunities, which is based on the seminal papers of M&M(1961, 1963) and Lee(2006). Making use of the estimation model, we estimate a new proxy variable of the growth opportunities, other than the usual proxy variables such as quasi Tobin's Q, MBR, and so on. The new proxy variable of growth opportunities can represent a necessary condition to identify whether the increase in new investments are successful or not. The empirical findings on the growth opportunities during the IMF currency crisis period and the post-IMF period are as follows; First, the overall success rate of new investments is as low as 50%, that means only half of the listed firms were increasing new investments when they had the growth opportunities. Second, during the crisis, one third of the listed firms were experiencing negative growth opportunities. However, during the post-IMF period, the growth opportunities on new investments were turned to be positive due to the turnaround efforts of listed firms. Third, the value of intangible assets, which are resulting from the investment of R&D and human capital, are becoming more important than ever. It seems to be true that larger portions of the value of growth opportunities are coming from the intangible assets, not from the increases in new investments of the physical assets.

• Korean Journal of Poultry Science
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• 제33권3호
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• pp.233-238
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• 2006

In order to study the effect of feeding rare earth(RE) on growth of broiler chicks, feed conversion ratio, and probable harmfulness of feeding high levels of RE, two feeding trials with broiler chicks were conducted; one using a commercial broiler compound feed and the other using a self mixed feed excluding any growth stimulating feed additives. The the first trial used three hundred sixty of one day old Cobb broiler chicks for six levels of dietary supplementation of RE : 0, 100, 200, 400, 800 and 1,600 mg/kg. There were significant effect of RE stimulating broiler chick growth and improving feed conversion ratio, The dietary supplementation of RE at a level of 100 mg/kg was the best of all and increased body weight gain by 8.9% (p<0.05). Chicks fed RE at a level of 1,600 mg/kg grew as good as chicks fed feeds without RE and did not show my abnormalities. The second feeding trial was conducted in the same manner as the first trial using Ross broiler chicks and self-mixed experimental diets supplemented with RE at levels of 0, 50, 100 and 150 mg/kg. Differently from Results of the first trial, body weight gain and feed conversion ratio of were the best at the level of 50 mg/kg RE supplementation(p<0.05). In this trial all the birds fed RE showed significantly lower ratio of abdominal fat against live weight than those of the control group birds. Overall, it can be said that dietary supplementation of RE will improve broiler growth and feed conversion ratio and the proper dietary level would be $50\sim100mg/kg$. These results suggested that the proper level of RE for broiler chicks would be 50 to 100 mg/kg and its effectiveness is varied depending upon RE mixture. There appears abdominal fat of broiler chicks is decreased by feeding RE but further investigation is in need.

• Radiation Oncology Journal
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• 제6권1호
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• pp.1-11
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• 1988

Recovery from potentially lethal damage (PLDR) after irradiation was studied in plateau-phase culture of Vero cells in vitro. Unfed plateau-phase cells were irradiated with dose of 1 to 9Gy using Cs-137 irradiator. Cells then were incubated again and left in situ for 0, 1, 2, 3, 4, 5, 6, and 24 hours and then were trypsinized explanted, and subcultured in fresh RPMI-1640 media containing $0.33\%$ agar. Cell survival was measured by colony forming ability. An adequate number of heavily irradiated Vero cells were added as feeder cells to make the total cell number constant in every culture dish. As the postirradiation in situ incubation time increased, surviving fraction increased by PLDR. The rate of PLDR was so rapid that increased surviving fraction reached saturation level at 2 to 4 hours after in situ incubation. As the radiation dose increased, the rate of PLDR fastened and the magnitude of increased surviving fraction at saturation level by PLOR also increased. In analysis of cell survival curve fitted to the linear-quadratic model, the linear inactivation coefficient $(\alpha)$ decreased largely and reached nearly to zero but the quadratic inactivation coefficient $(\beta)$ increased minimally by increment of postirradiation in situ incubation time. So PLDR mainly affected the damage expressed as $\alpha$, In the multitarget model, significant change was not obtained in $D_0\;but\;in D_q$. Therefore, shoulder region in cell survival curve was mainly affected by PLDR and terminal slope was not influenced at all. And dose-modifying factor by PLDR was relatively higher in shoulder region, that is, in low dose area below 3 Gy.

• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3403-3408
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• 2012

The molecular mechanisms involved in the progression of clear cell renal cell carcinomas (ccRCCs) are still unclear. The aim of this study was to analyse the relationships between expression of RALYL and clinical characteristics. In 41 paired samples of ccRCCs and adjacent normal tissues, we used real-time qPCR to evaluate the expression of RALYL mRNA. RALYL protein levels were determined in 146 samples of ccRCC and 37 adjacent normal tissues by immunohistochemistry. Statistical analysis was used to explore the relationships between expression of RALYL and the clinical characteristics (gender, age, tumor size, T stage, N stage, M stage, survival times and survival outcome) in ccRCC. In addition, these patients were follow-up period 64 months (range: 4~116months) to investigate the influence on prognosis. We found significantly differences between ccRCC tissues and normal tissues (p<0.001, paired-sample t test) in mRNA levels of RALYL. Immunohistochemistry analyses in 146 ccRCC samples and 37 adjacent normal tissues showed significantly lower RALYL protein levels in ccRCC samples (${\chi}^2$-test, p<0.001), inversely correlating with tumour size (p=0.024), T stage (0.005), N stage (p<0.001) as well as M stage (p=0.019), but not age (p=0.357) and gender (p=0.348). Kaplan-Meier survival analysis demonstrated that people with lower level of RALYL expression had a poorer survival rate than those with a higher level of RALYL expression, significantly different by the log-rank test (p=0.011). Cox regression analysis indicated that RALYL expression (p=0.039), N stage (p=0.008) and distant metastasis (p<0.001) were independent prognosis factors for the overall survival of ccRCC patients. We demonstrated that the expression of RALYL was significantly low in ccRCC and correlated with a poor prognosis in a large number of clinical samples. Our findings showed that RALYL may be a potential therapeutic target as well as a poor prognostic factor.

• Journal of Korean Society of Forest Science
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• 제80권3호
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• pp.279-286
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• 1991

This study was carried out to investigate relationships between atmospheric $SO_2$ concentration and both soluble sulphur contents in the tree leaves and bark pH to test the possibility of using them as bioindicators for air pollution. Mt. Kwanak, Mt. Nam, Mt. Bukhan (located in Seoul) and Mt. Paldal (located in Suwon, Kyonggi-do) as polluted areas and Pyongchang, Kwangwon-do as an unpolluted area were selected for this study. Soluble sulphur contents in the leaves and hark pH of two tree species (Pines densiflora S. et Z. and Quercus mongolica Fisch.) were analyzed in May, August, and October, 1990 by $BaSO_4$ precipitation method and pH measurement, respectively. In both species, concentration of soluble sulphur in the leaves increased with increasing concentration of atmospheric $SO_2$ (correlation coefficient : 0.52). Soluble sulphur contents in the 2-year-old needles of Pines densiflora (0.170%) and current year leaves of Quercus mongolica (0.081%) in Mt. Nam in the center of Seoul were higher than those in unpolluted Pyongchang area (0.023% and 0.034%, respectively). Bark pH decreased with increasing atmospheric $SO_2$ concentration (correlation coefficient : -0.52). Bark pH of P. densiflora (pH 3.42) and Q. mongolica (pH 3.63) in Mt. Nam were lower than those in Pyongchang area (pH 3.94 and pH 4.93, respectively). Both soluble S content in the leaves and bark pH were recognized as suitable bioindicators for air pollution by $SO_2$. Especially, bark pH showed more sensitive response to air pollution by $SO_2$ than soluble S concentration in the leaves. The lowest concentration of soluble sulphur and the highest bark pH in August were considered to be due to heavy rain during the rainy season. Soluble S content in the leaves and bark pH were not significantly different at 5% level between the two species in polluted areas.