• Journal of the Korean Institute of Landscape Architecture
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• v.30 no.2
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• pp.95-104
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• 2002

This study was carried out to investigate the effects of pyroligneous acid on turfgrass growth and to propose usage for sustainable management on Korean golf course green. Each plot was treated with 0, 300, 500, and 800 acid to water solution. On all the plots, turfgrass growth increment and visual quality were measured at fried times. The data were subjected to paired samples t-test and corelation analysis. The summarized results are as follows; 1) Leaf growth increment, density, and root spread depth of turfgrass treated with 1: 500 and 1: 800 diluted pyroligneous acid were significantly superior to the control. Particularly, 1: 500 diluted solution was superior to the others. 2) Color, texture, and uniformity of turfgrass treated with 1: 500 and 1: 800 diluted pyroligneous acid were significantly superior to the control. Especially, 1: 500 diluted solution tended to be superior to the others. 3) There were no symptoms of disease in all plots treated with the diluted pyroligneous acid. 1 500 and 1: 800 diluted pyroligneous acid were not thought to trigger or promote disease. 4) Before and after investigating the effect of pyroligneous acid on turfgrass growth ,the soils were analyzed. In all treatments, the chemical properties of the soils did not change noticeably. The chemical properties of the soils might be not changed by the diluted pyroligneous acid treatments. 5) Considering the results mentioned above, 1: 500 diluted pyroligneous acid was the most effective. Though pyroligneous acid is an organic fertilizer and contains only a little nitrogen and phosphorus, 1: 500 diluted pyroligneous acid promoted turfgrass growth effectively. Thus this use might lead to a reduction in the amount of fertilizers used and result in ecologically responsive management of Korean golf courses.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.1
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• pp.97-100
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• 2014

To explore the possibility of using pyroligneous acid for environmentally friendly ginseng farming, this study observed samples of ginseng whose shoots were treated with pyroligneous acid sprays beginning in mid June, which is after foliation stage. The spongy tissue structure got thickened from triple layers to quadruple layers with the pyroligneous acid regardless of the concentration. The upper and lower epidermis cell of the leaves as well as the leaf mesopyll cells also became thicker. Compared to the no-treatment group, the overall growth and development of ginseng roots treated with pyroligneous acid were excellent. Accordingly, it is believed that pyroligneous acid can be an environmentally friendly alternative to conventional agro-chemicals applied to ginseng that can be used to facilitate the growth and development of ginseng.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.164-169
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• 2013

Alternatives to commercial chemical herbicide are currently being searched and tested due to the numerous adverse effects of commercially available herbicides to the environment. Barnyardgrass (Echinochloa crusgalli) is an important weed species around the world, especially in paddy rice fields. This study focuses on the favorable effects of mixing pyroligneous acids with commercial liquid herbicides. Seedlings were transplanted and grown under greenhouse conditions. The effect of treatment time or leaf-stage on herbicide-pyroligneous acid efficacies was checked, coupled with isolation and quantification of biochemical compounds. Results revealed that herbicide treatment at early post emergence (2~3 leaf stage) of Echnochloa crus-galli leads to effective control. Both liquid herbicides affected fatty acid, protein, and amino acid syntheses as reflected on their contents. The influence of wood vinegar (WV) or rice vinegar (RV) on these compounds was not thoroughly verified due to lack of information on the pyroligneous products. We observed that mixing WV or RV with BCB (bentazone + cyhalof-butyl) gives more favorable results than BUC (butachlor + clomazone), mixed with WV or RV. The result would indicate the potential of mixing pyroligneous acid in reducing herbicide application rate.

• Asian Journal of Turfgrass Science
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• v.19 no.2
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• pp.73-83
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• 2005

This study was carried out to investigate control efficacy of fungicides mixed with pyroligneous acid on Large patch caused by Rhizoctonia solani AG2-2. R. solani AG2-2 was not inhibited by concentration of 500 times and 1,000 times diluents of pyroligneous acid. It is thought that R. solani AG2-2 was not inhibited by Pyroligneous acid alone. Pencycuron and tebuconazol mixed with pyroligneous acid had a good effect in prevention of R. solani AG2-2 in zoysiagrass. Concurrently, it was observed that those were reduced $50\%$ of usage of fungicides. 500 times and 1,000 times diluents of pyroligneous acid treated group had a significantly effect compared to control turf in the leaf length, leaf width and dry weight. In conclusion, usage of fungicides could be reduced about $50\%$ by a fungicides mixed with pyroligneous acid for protection of R. solani AG2-2. Therefore, It is thought that fungicides mixed with pyroligneous acids can be reduced an economic load and also an environmental pollution such as a tolerance to pathogenic bacteria and a destruction of an ecological system of soil microbes according to use fungicides.

• Food Science of Animal Resources
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• v.18 no.1
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• pp.75-80
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• 1998

pH in sausages with 0.5% and 2% pyroligneous liquor was lowered rapidly respectively. Sausage with 2% pyroligneous liquor showed the lowest Aw. Antioxidant activity of 0.02% and 0.5% pyroligneous liquor was negligible but rancidity was inhibited in the sausages with 2% pyroligneous liquor and 0.02% BHA/BHT(1:1) Sensory tests showed that sausages treated with pyrolignesous liquor had lower score. The growth of lactic acid bacteria was not affected by pyroligneous liquor. But enterobacteria was not detected after 10 days fermentation of the sausages wit 0.02% BHA/ BHT 8 days in the 0.5% pyroligneous liquor and 6 days in the 2% pyroligneous liquor.

• Proceedings of the Turfgrass Society of Korea Conference
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• 2002.02a
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• pp.17-17
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• 2002

• Journal of Korea Foresty Energy
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• v.22 no.3
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• pp.49-56
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• 2003

As a part of agricultural utilization of charcoal and pyroligneous acid, the effect of wood carbonization products on the growth of red pepper and soil microorganisms was investigated. The treatment of charcoal and pyroligneous acid provided good growth conditions to microorganisms through neutralizing soil acidity and improving the physicochemical properties of soil. Therefore the density of useful microorganism in the soil has been increased. In the growth of red pepper, the length, diameter, and the fruit numbers of red pepper have been increased by treating with wood carbonization products. It was especially shown that yield has increased about 50% in the fruit number, by treating charcoal 1kg, 1000 time-diluted solution of pyroligneous acid and bacteria, compared with the control. It was estimated that increasing the length of seedling and the diameter of red pepper stem contributed to the resistance against the prerequisites of various environmental changes in open field. Therefore, the final yield would be increased. In the antagonism experiment of red pepper mold (Colletotrichum gloeosporioides), the mold became extinct in the 2- and 10-time diluted solution of pyroligneous acid, compared with the control. On the other hand, their growth speed was delayed in the 100- and 1000 time-diluted solution.

• Asian Journal of Turfgrass Science
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• v.15 no.4
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• pp.157-168
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• 2001

This study was carried out to investigate the offsets of pyroligneous acid on the growth of ‘Penncross’Creeping Bentgrass (Agrostis palustris Huds.) and to propose the practical use for ecologically responsive management in Korean golf courses. In the plots affected by 300, 500, and 800 times diluted pyroligneous acid, the turfgrass growth increment and the visual quality were measured at fixed periods. The data were subjected to paired samples T-test. The results summarized are as follows； 1) In leaf growth increment, density, and root spread depth, turfgrass affected by 500 and 800 times diluted pyroligneous acid were superior to the control continually. It was reliable in the statistics. Especially, 500 times diluted solutions was superior to the others. 2) In color, texture, uniformity, and recuperative strength, the visual quality of turfgrass affected by 500 and 800 times diluted pyroligneous acid were superior to the control continually. It was reliable in the statistics except the recuperative strength. Espe-cially, 500 times diluted solutions tended to be superior to the others. 3) There was no symptom of a disease in all plots. The existing management was thought to be suitable in this golf course. 500 and 800 times diluted pyroligneous acid were not thought to trigger or promote a disease, 4) After investigating the effect of pyroligneous acid on turfgrass growth, the soils of all plots were analyzed. In all plots, the chemical properties of the soils were not changed obviously. 5) Considering the results mentioned above, the practical use of 500 and 800 times diluted solutions might decrease the amount of nutrients and agricultural chemicals used in golf courses as well as promote turfgrass growth in golf courses.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.673-677
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• 2005

This experiment was conducted to clarify the effect of pyroligneous liquor on endogenous substances that were GA (gibberelic acid)-like substances, starch, and protein of Neofinetia falcata cultured in vitro. When seedlings of Neofinetia falcata were treated with several concentrations (0, 0.5, 1.0, 2.0, 3.0 ml/L) of pyroligneous liquor, the growth of seedlings was enhanced in 1.0ml/L pyroligneous liquor added media. The activity of GA-like substances was lower in the leaf, but higher in the and root than that of the control. The content of starch in 1.0ml/L pyroligneous liquor was lower in the leaf, but higher in the root than that of the control. And the content of protein in 1.0ml/L pyroligneous liquor was also lower in the leaf, but higher in the root than that of the control.

• Korean Journal of Environmental Agriculture
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• v.30 no.4
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• pp.453-458
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• 2011

BACKGROUND: Coleosporium plectranthi, an obligate parasite, which is responsible for the rust disease of Perilla frutescens, a plant in Korea, commonly known as Perilla. All rusts are obligate parasites, meaning that they require a living host to complete their life cycle. They generally do not kill the host plant but can severely reduce growth and yield. Food and feed spoilage fungi cause great economic losses worldwide. It is estimated that between 5 and 10% of the world food production is wasted due to fungal deterioration. Rust disease of Perilla is highly frequent and is widely spread in Korea. The present study was designed to investigate a novel media for the urediniospore germination in vitro and anti-rust activity as well as GC-MS analysis of oak pyroligneous liquor. METHOD AND RESULTS: Urediniospores were collected from the infected leaf of Perilla. Spore suspension was made and the suspension was inoculated in the 2% water agar media with proper humidity, then they were incubated at $26^{\circ}C$ for 56 hrs. The GC-MS analysis of the oak pyroligneous liquor was also done to check the chemical composition. GC-MS analysis of the wood vinegar was found 15 compounds, among them o-mthoxyphenol (25.93%), 2,6-dimethoxyphenol (16.06%), 4-methylenecyclohexanone (10.69%), 2,3-dihydroxytoluene (7.84%), levoglucosane (6.14%) and propanoic acid (5.32%) were the major components. Different concentration of the oak pyroligneous liquor was used, and spore inhibition was recorded on the basis of spore counting. The best results were noted at the concentration of 50% solution where 31.8% spores were inhibited. CONCLUSION: On the basis of the chemical composition of the oak pyroligneous liquor and the activity recorded we can use it as an anti-rust agent.