• 한국동물학회지
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• 제16권1호
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• pp.13-23
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• 1973

저자들은 성숙한 雄性白鼠(Albino Wistar)에 INH 40mg/kg, pyridoxine 20mg/kg를 경구적으로 투여하여 mast cell과 enterochromaffin cell 의 변동을 관찰하여 다음과 같은 결과를 얻었다. 1. INH투여로 白鼠 약 舌 mast cell 의 심한 파괴와 細胞質顆粒의 逸出作用으로 세포수는 격감하였으나, pyridoxine 투여군에서는 대조군에 비해 상당한 수적증가를 보였다. 2. INH 투여로 白鼠 심이지장 enterochromaffin cell의 세포수와 細胞質顆粒양에 현저한 감소를 나타냈으나 pyridoxine 투여군에서는 대조군에 비해 enterochromaffin cell의 상당한 수적증가를 보였다. 3. 이상의 성적에 있어서 INH의 다량 투여는 mast cell과 enterochromaffin cell의 생성에 심한 장해를 주며 pyridoxine 의 적당한 투여는 mast cell과 enterochromaffin cell의 생성을 조장 하고 顆粒양을 증가시키는 것으로 보아 이들 세포의 分泌産物인 5-Hydroxytryptamine 대사에 pyridoxine이 관여하는 것으로 사료된다.

• Journal of Nutrition and Health
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• 제20권5호
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• pp.318-329
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• 1987

이유한 Sprague Dawley 암컷 쥐에게 성장, 임신, 수유기간 동안 Pyrridoxine이 충분한 식이와 Pyridoxine 이 부족된 식이를 주어 사육했으며, 또는 다른 군은 성장, 임신기간은 pyridoxine이 부족된 식이를 공급받아가 생후 5, 10, 21일에 각각 pyridoxine 이 풍부한 식이로 바꾸어 주어회복정도를 살펴보았다. 출생후, 5, 10, 15, 21,35, 50 일에 체중을 재고 새끼를 희생시켜 뇌와 적혈구를 실험에 사용하였다. 적혈구에서는 alanine aminotransferase 활성을 측정하였고, 뇌에서는 cholesterol, proteolipid protein, cerebroside를 측정하였다. Pyridoxine이 부족된 쥐는 실험기간동안 유의적으로 체중과 뇌무게가 감소했으며, erythrocyte alanine aminotransferase 활성의 증가정도가 훨씬 높은 것으로 보아 체내 비타민 B6 의 영양상태가 나쁨을 알 수 있었다. 할편, 뇌의 ceregroside의 gakfid은 유의적인 차리를 보였으며 5, 10, 21일에 supplementation 시킨 쥐의 이유후의 cerbroside 의 생성속도의 증가로 보아 supplementation 시킨 쥐의 myelinatino 이 저해되었음을 알수 있다. 한편, cholesterol, proteolipid protein 도 부족식이에 의해 영향을 받았는데 생후 5 일에 supplementation 시킨 쥐는 정상수준으로 회복되나 10 일 이후에 supplementation 시켰을대는 정상수준에 미치지 못하였다.

• 대한치과의사협회지
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• 제10권4호
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• pp.209-212
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• 1972

The effect of pyridoxine on the incisors were histochemically studied for the purpose of identifying the phosphoric acid. The microscopic observations were carried out by means of phosphoric acid reaction(Serraet Queiroz-Lopes method), mucoprotein reaction (Clara method), periodic acid-Schiff reaction (McManus method), methylen blue stain and hematoxylin-eosin stain. In the experimental result, it was found that phosphoric acid reactions of ameloblast, Tomes' process and preenamel were remarkably increased after pyridoxine administration.

• BMB Reports
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• 제39권1호
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• pp.76-83
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• 2006

Pyridoxine-5-P oxidase catalyses the terminal step in the biosynthesis of pyridoxal-S-P, the biologically active form of vitamin $B_6$ Which acts as an essential cofactor. Here, a human brain pyridoxine-5-P oxidase gene was fused with a gene fragment encoding the HIV-1 Tat protein transduction domain (RKKRRQRRR) in a bacterial expression vector to produce a genetic in-frame Tat-pyridoxine-5-P oxidase fusion protein. Expressed and purified Tat-pyridoxine-5-P oxidase fusion protein transduced efficiently into PC12 cells in a time- and dose-dependent manner when added exogenously to culture media. Once inside the cells, the transduced Tat-pyridoxine-5-P oxidase protein showed catalytic activity and was stable for 48 h. Moreover, the formation of pyridoxal-5-P was increased by adding exogenous Tat-pyridoxine-5-P oxidase to media pre-treated with the vitamin $B_6$ precursor pyridoxine. In addition, the intracellular concentration of pyridoxal-S-P was markedly increased when Tat-pyridoxal kinase was transduced together with Tat-pyridoxine-5-P oxidase into cells. These results suggest that the transduction of Tat-pyridoxine-5-P oxidase fusion protein presents a means of regulating the level of pyridoxal-5-P and of replenishing this enzyme in various neurological disorders related to vitamin $B_6$.

• Asian-Australasian Journal of Animal Sciences
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• 제27권12호
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• pp.1744-1748
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• 2014

A dose-response experiment with seven supplemental pyridoxine levels (0, 0.66, 1.32, 1.98, 2.64, 3.30, and 3.96 mg/kg) was conducted to investigate the effects of pyridoxine on growth performance and plasma aminotransferases and homocysteine of White Pekin ducks and to estimate pyridoxine requirement for these birds. A total of 336 one-day-old male White Pekin ducks were divided to 7 experimental treatments and each treatment contained 8 replicate pens with 6 birds per pen. Ducks were reared in raised wire-floor pens from hatch to 28 d of age. At 28 d of age, the weight gain, feed intake, feed/gain, and the aspartate aminotransferase, alanine aminotransferase, and homocysteine in plasma of ducks from each pen were all measured. In our study, the pyridoxine deficiency of ducks was characterized by growth depression, decreasing plasma aspartate aminotransferase activity and increasing plasma homocysteine. The ducks fed vitamin $B_6$-deficient basal diets had the worst weight gain and feed/gain among all birds and this growth depression was alleviated (p<0.05) when pyridoxine was supplemented to basal diets. On the other hand, plasma aspartate aminotransferase and homocysteine may be the sensitive indicators for vitamin $B_6$ status of ducks. The ducks fed basal diets had much lower aspartate aminotransferase activity and higher homocysteine level in plasma compared with other birds fed pyridoxine-supplemented diets (p<0.05). According to quadratic regression, the supplemental pyridoxine requirements of Pekin ducks from hatch to 28 days of age was 2.44 mg/kg for feed/gain and 2.08 mg/kg for plasma aspartate aminotransferase and the corresponding total requirements of this vitamin for these two criteria were 4.37 and 4.01 mg/kg when the pyridoxine concentration of basal diets was included, respectively. All data suggested that pyridoxine deficiency could cause growth retardation in ducks and the deficiency of this vitamin could be indicated by decreasing plasma aspartate aminotransferase activity and increasing plasma homocysteine.

• Journal of Nutrition and Health
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• 제19권4호
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• pp.246-254
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• 1986

이유한 Sprague Dawley 종의 암컷 흰쥐를 두군으로 나누어 각각 pyridoxine이 충분한 식이 (22mg/kg diet)와 pyridoxine이 부족한 식이 (1.2mg/kg diet0로 성장시킨후, 적절한 시기에 임신시켰다. 태어난 새끼가 이유할 때 결핍식이군을 둘로 나누어, 한쪽은 pyridoxine이 충분한 식이로 바꾸어준후, 세군의 쥐를 생후 10-주까지 성장시켰다. 각 군의 새끼쥐가 각각 0, 3, 7, 10 주 되었을대, 간의 mitochondria 및 cytosolic fraction 에 있는 Asparate aminotransferase [ EC 2.6.1.1] activity 및 pyridoxal phosphate의 함량을 측정하였다. 결핍군의 간 aspartate aminotransterase activity는 mitochondria 및 cytosolic fraction에서 각각 대조군에 비해 유의적으로 낮았으며 10-4 M pyridoxal phosphate를 첨가한 후 측정한 total enzyme activity 는 거의 대조군과 비슷하였다. 3 주이후 pyridoxine 이 충분한 식이로 바구어준 회복군에서도 대조군에 비해 유의적으로 낮았으나, 결핍군보다는 높았다. 이두 fraction 에서의 pyridoxal phosphate 함량은 결핍군이 대조군보다 현저히 낮았으며, 회복군은 대조군과 비슷하였다. 이로써, 장기간에 걸친 pyridoxine결핍이간의 aspartate aminotransferase activity 및 pyridoxal phosphate에 현저한 영향을 미치며 이유기 이후의 식이보충에 의해서도 enzyme activity는 쉽게 회복되지 않음을 알 수있다.

• 한국식품과학회지
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• 제9권3호
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• pp.183-189
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• 1977

고정화(固定化) 균체(菌體)를 이용(利用)한 pyridoxal 5'-phosphate(pyridoxal-p)의 연속생산(連續生産)에 관(關)해실험(實驗)하였다. pyridoxine 5'-phosphate (pyridoxine-p) oxidase활성(活性)을 갖는 Pseudomonas polycolor 균체(菌體)와 Catalase 활성(活性)을 갖는 Kloeckera sp. No. 2201균체(菌體)를 효소원(酵素源)으로 사용(使用)하였다. 균체(菌體)는 Polyacrylamide gel에 불용화(不溶化) 시켰으며 동시고정균체(同時固定菌體)의 활성(活性)이 Pseudomonas Polycoler단독고정균체(單獨固定菌體)의 활성(活性)보다 강(强)하였다. 이 결과(結果)는 동시고정균체(同時固定菌體)의 Pyridoxine-p oxidase-cat-alase system은 Pyridoxine-p 산화(酸化)의 부산물(副産物)인 $H_2O_2$를 분해(分解)하므로서 보호효과를 얻을 수 있음을 의미(意味)한다. 동시고정균체(同時固定菌體)와 생균체(生菌體)의 효소활성(酵素活性)에 미치는 최적(最適)pH는 9.0이었고 동시고정균체(同時固定菌體)의 최적온도(最適溫度)는 $45^{\circ}C$로 생균체(生菌體)보다 $5^{\circ}C$높았다. 고정균체(固定菌體)의 Pyridoxine-p oxidase는 $Hg^{2+}$와 몇몇 SH-화합물(化合物)에 의(依)하여 활성화(活性化)되었다.

• Journal of Medicine and Life Science
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• 제21권1호
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• pp.11-14
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• 2024

Excess of pyridoxine, in contrast to other nutrients, may result in neuropathy. Case reports are sparse, and little is known about the clinical and electrophysiological findings. Eight patients with pyridoxine-induced neuropathy were investigated, and a review of the literature was undertaken. Nerve conduction studies showed axonal sensory or sensorimotor polyneuropathy. And the blood levels of vitamin B6 were markedly elevated. After discontinuation of vitamin supplements, all patients showed no significant improvement in clinical and electrophysiological findings. Supplementation with pyridoxine at doses greater than 50 mg/day for extended durations may be harmful and should be discouraged.

• Journal of Nutrition and Health
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• 제17권3호
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• pp.199-209
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• 1984

Pregnant rats were fed a pyridoxine deficient diet during the gestation and lactation. DEF I group received the deficient diet from delivery ; DEF II group, from the 15 th day of gestation. Body and brain weights, brain protein, DNA, RNA, plasma GOT and GPT, and catecholamines were measured. Effect of MAO inhibiting drug, pargyline, was determined. Brain protein, DNA, and RNA of offsprings of deficient groups were significantly lower than the control group, but RNA/ DNA, brain weight/DNA, and protein/DNA show that cell number were more affected than cell size by the pyridoxine deficiency during the 3rd week of gestation and lactation. Plasma GOT activities were more significantly different than plasma GPT between the control and deficient group. Brain norepinephrine of offsprings of deficient group were significantly lower than the control, but brain dopamine content was not significantly different from the control. At 2nd and 3rd week, norepinephrine was significantly depressed in deficient groups. Pargyline treatment affected a 1.2 fold increase in catecholamines in 3hr while the control had a 1.5 fold increase. Thus norepinephrine and dopamine synthesis was depressed in the deficient groups. Dopaminergic neurons may be less dependent on pyridoxine level than neurons from norepinephrine. Pyridoxine deficiency in maternal diet is not so critical to brain catecholamines of offspring except to the neonatal rats.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2004년도 제21차 정기총회 및 학술발표회
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• pp.93-94
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• 2004

The experiments was conducted to investigate the effects of dietary supplementation of feather meal and pyridoxine on the taurine content of egg yolk and performance of laying hens. Feeding trial was conducted with 900 31-wk-old Hy-Line Brown layers for 4wks. The experiment consisted of six dietary treatments: control(basal diet), feather meal(FM) 3 % diet(FM 3 %), FM 3 % + pyridoxine supplemented diet(FM 3 % + Pyridox), FM 6 % diet, FM 6 % + pyridoxine supplemented diet(FM 6% + Pyridox), Synthetic taurine 0.25 % supplemented diet(Taurine). Egg production of birds fed FM 3% was highest and those of the FM diets were also higher than those of Taurine and the control. Egg weight of Taurine was significantly lower than those of FM 3 %, FM 6 % and the control but were not significantly different from those of FM 3 % + Pyridox or FM 6 % + Pyridox. Feed intake of the control was greater than those of FM 6 %, FM 6% + Pyridox or Taurine treatment but was not significantly different from those of FM 3 % and FM 3 % + Pyridox. Feed conversion of the control was significantly higher than other treatments in which that of FM 6 % was lowest. Broken and soft egg production of Taurine was highest while that of the control was lowest among treatments. Taurine content of egg yolk significantly increased by supplementation of taurine(64.7 %). FM 6 % + pyridoxine(57 %), FM 3% + pyridoxine (32.1%) and FM 6% (16.6 %). Sensory evaluation data of Taurine has shown the highest score in most of sensory attributes. It is concluded that taurine can be enriched in egg yolk by supplementation of 6 % FM diet and pyridoxine as well as 0.25 % synthetic taurine.