• 제목/요약/키워드: Pyrenophora graminea

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Vegetative Compatibility Groups and Virulence Variation Among Isolates of Pyrenophora graminea

  • Arabi, Mohammad Imad Eddin;Jawhar, Mohammad
    • The Plant Pathology Journal
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    • 제27권2호
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    • pp.116-119
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    • 2011
  • Pyrenophora graminea, the causal agent of leaf stripe disease, is an economically important pathogen of barley found worldwide. Forty-four isolates of diverse geographical origin within Syria were grouped into vegetative compatibility groups (VCGs) by demonstrating heterokaryosis by complementation tests using nitrate nonutilizing (nit) mutants. All isolates were grouped into three VCGs-1-A, 1-B and 1-C. No self-incompatibility was observed in any of the isolates tested. VCG 1-A was the most common group within growing regions in Syria and proved to be the most virulent of the VCGs identified. These data indicate that the level of virulence in P. graminea is related to VCG.

맥류(麥類)에 발생(發生)하는 Ustilago nuda 및 Pyrenophora graminea에 대한 종자(種子) 소독(消毒)에 관한 연구(硏究) (Control of Seed-borne Infection of Ustilago nuda and Pyrenophora graminea on Barley)

  • 이두형
    • 한국균학회지
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    • 제8권2호
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    • pp.89-94
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    • 1980
  • 맥류(麥類)의 겉깜부기병(라흑종병(裸黑種病))과 줄무늬병(반엽병(班葉病))에 대한 종자(種子) 소독(消毒) 효과(效果)를 보기 위하여 자연이병(自然罹病)된 종자(種子)에 Baytan외(外) 13종(種)의 엽제(葉劑)를 처리(處理)한 다음 포장(圃場)에 파종(播種)하여 발병상황(發病狀況)을 조사(調査)하였다. Sisthane 및 Benlate T는 겉깜부기병과 줄무늬병에 대하여 Vitathiram과 같은 방제효과(防除效果)를 나타냈으며, Baytan U는 겉깜부기병에는 효과적(效果的)이었으나 줄무늬병에는 효과(效果)가 떨어졌다. P242는 줄무늬병에는 좋은 약효(藥效)를 나타냈으나 겉깜부기병의 발생(發生)을 막는데는 효과(效果)가 적었으며, Busan 30은 겉깜부기병과 줄무늬병에 대하여 Vitathiram에 비해서 효과(效果)가 불안정(不安定)하였다. 홍시약제(洪試藥劑)로 처리한 보리 쌀보리 및 밀을 실내(室內)서에 발아상(發芽箱)에 키웠을 때 Sisthane과 Baytan U의 고농도처리구(高濃度處理區)에서는 뿌리뒤틀림 현상으로 생육(生育)의 저해(沮害)가 나타나지 않았다.

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Characterization of Pyrenophora graminea Markers Associated with a Locus Conferring Virulence on Barley

  • Mokrani, Lubna;Jawhar, Mohammad;Shoaib, Amina;Arabi, Mohammad Imad Eddin
    • The Plant Pathology Journal
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    • 제28권3호
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    • pp.290-294
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    • 2012
  • The fungus Pyrenophora graminea is the causal agent of barley leaf stripe disease. Two leaf stripe isolates PgSy3 (exhibiting high virulence on the barley cultivar 'Arabi Abiad') and PgSy1 (exhibiting low virulence on Arabi Abiad), were mated and 63 progeny were isolated and phenotyped for the reaction on Arabi Abiad. The population segregated in a 1:1 ratio, 32 virulent to 31 avirulent (${\chi}^2$ = 0.05, P = 0.36), indicating single gene control of PgSy3 virulence on Arabi Abiad. Among 96 AFLP markers identified, three AFLP markers, E37M50-400, E35M59-100 and E38M47-800 were linked to the virulence locus VHv1 in isolate PgSy3. The results of this study indicate that (the three markers) are closely linked to VHv1 and are unique to isolates carrying the virulence locus. This work represents an initial step towards map-based cloning of VHv1 in P. graminea.

Alternative Measure for Assessing Incidence of Leaf Stripe on Barley

  • Arabi, Mohammad Imad Eddin;Jawhar, Mohammad
    • The Plant Pathology Journal
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    • 제28권2호
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    • pp.212-215
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    • 2012
  • Pyrenophora graminea, the causal agent of leaf stripe disease, is an economically important pathogen of barley found worldwide. It is critical to clearly define and standardize the leaf stripe assessment methods to avoid subjectivity and variability between assessors. Therefore, in this study, a comparison between the proportion of diseased plants (DP) and the proportion of diseased leaves (DL) per plant was investigated. Disease assessments were made visually at multiple sample sites in artificially and naturally inoculated research and production fields during four growing seasons. There were significant differences (P = 0.001) among cultivars in mean DP and DL averages, which are consistently higher in susceptible barley. However, DP values increased linearly as DL increased. The slopes and intercepts of the DP-DL relationship were consistent over the four growing seasons. This result might make a significant contribution for leaf stripe assessment in barley breeding programme.