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http://dx.doi.org/10.5423/PPJ.NT.11.2011.0217

Characterization of Pyrenophora graminea Markers Associated with a Locus Conferring Virulence on Barley  

Mokrani, Lubna (Department of Molecular Biology and Biotechnology)
Jawhar, Mohammad (Department of Molecular Biology and Biotechnology)
Shoaib, Amina (Department of Molecular Biology and Biotechnology)
Arabi, Mohammad Imad Eddin (Department of Molecular Biology and Biotechnology)
Publication Information
The Plant Pathology Journal / v.28, no.3, 2012 , pp. 290-294 More about this Journal
Abstract
The fungus Pyrenophora graminea is the causal agent of barley leaf stripe disease. Two leaf stripe isolates PgSy3 (exhibiting high virulence on the barley cultivar 'Arabi Abiad') and PgSy1 (exhibiting low virulence on Arabi Abiad), were mated and 63 progeny were isolated and phenotyped for the reaction on Arabi Abiad. The population segregated in a 1:1 ratio, 32 virulent to 31 avirulent (${\chi}^2$ = 0.05, P = 0.36), indicating single gene control of PgSy3 virulence on Arabi Abiad. Among 96 AFLP markers identified, three AFLP markers, E37M50-400, E35M59-100 and E38M47-800 were linked to the virulence locus VHv1 in isolate PgSy3. The results of this study indicate that (the three markers) are closely linked to VHv1 and are unique to isolates carrying the virulence locus. This work represents an initial step towards map-based cloning of VHv1 in P. graminea.
Keywords
AFLP markers; barley; leaf stripe; Pyrenophora graminea; virulence gene;
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1 Tekauz, A. 1983. Reaction of Canadian barley cultivars to Pyrenophora graminea, the incitant of leaf stripe. Can. J. Plant Pathol. 5:294-301.   DOI
2 Vale, G., Aragona, M., Torrigiani, E., Cattivelli, L., Statanca, A., Porta-Puglia, A. and Delogu, G. 1994. Characterization of a hypo-virulent insertional mutant of Pyrenophora graminea and analysis of the barley defense response after inoculation. Plant Pathol. 47:657-667.
3 Vos, P., Hogers, R., Bleeker, M., Reijans, M. and Van de Lee, T. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23:4407-4414.   DOI   ScienceOn
4 Weiland, J. J., Steffenson, B. J., Cartwright, R. D. and Webster, R. K. 1999. Identification of molecular genetic markers in Pyrenophora teres f. teres associated with low virulence on 'Harbin' barley. Phytopathology 89:176-181.   DOI   ScienceOn
5 Zadoks, J. C., Chang, T. T. and Konzak, C. F. 1974. A decimal code for the growth stages of cereals. Weed Res. 14:415-421.   DOI
6 Lin, X., Huang, J. C., Mitchell, T. G. and Heitman, J. 2006. Virulence attributes and hyphal growth of C. neoformans are quantitative traits and the MAT$\delta$ allele enhances filamentation. PLoS Genet. 11:1801-1814.
7 Lind, M., Kerstin, D., Jan, S., Bo, K. and Ake, O. 2007. Identification of quantitative trait loci affecting virulence in the basidiomycete Heterobasidion annosum. Curr. Genet. 1:35-44.
8 Mathre, D. E. 1997. Compendium of barley diseases. American Phytopathological Society. pp. 120.
9 Michelmore, R. W., Paran, I. and Kesseli, R. V. 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA 88: 9828-9832.   DOI   ScienceOn
10 Paterson, A. H. 1996. Making genetic maps. In: Genome mapping in plants, ed. by A. H. Paterson, pp. 23-39, Landes Company, San Diego, California, Academic Press, Austin, Texas, USA.
11 Pecchioni, N., Faccioli, P., Toubia-Rahme, H. and Terzi, V. 1996. Quantitative resistance to barley leaf stripe (Pyrenophora graminea) is dominated by one major locus. Theor. Appl. Genet. 93:97-101.   DOI   ScienceOn
12 Porta-Puglia, A., Delogu, G. and Vannacci, G. 1986. Pyrenophora graminea on winter barley seed: effect on disease incidence and yield losses. Phytopathology 117:26-33.   DOI
13 Skou, J. P., Nielsen, B. J. and Haahr, V. 1994. Evaluation and importance genetic resistance to leaf stripe in western European barleys. Acta Agri. Scand. 44:68-106.
14 Smedegaard-Petersen, V. 1987. Genetics of heterothallism in Pyrenophora graminea and P. teres. Transact. British Mycol. Soc. 70:170-177.
15 Tacconi, G., Cattivelli, L., Faccioli, P., Toubia-Rahme, H., Vannacci, G., Stanca, A. M. and Vale, G. 2001. Identification and mapping of new leaf stripe resistance gene in barley (Hordeum vulgare L.). Theor. Appl. Genet. 102:1286-1291.   DOI
16 Arabi, M. I. E, Jawhar, M., Al-Safadi, B. and MirAli, N. 2004. Yield response of barley to leaf stripe (Pyrenophora graminea) under experimental conditions in southern Syria. J. Phytopathol. 152:519-523.   DOI   ScienceOn
17 Arabi, M. I. E., Jawhar, M. and MirAli, N. 2005. Storage protein (hordein) patterns of barley-Pyrenophora graminea interaction. Seed Sci. Technol. 33:409-418.   DOI
18 Arabi, M. I. E. and Jawhar, M. 2007. Inheritance of virulence in Pyrenophora graminea. Austr. Plant Pathol. 36:373-375.   DOI   ScienceOn
19 Delogu, G., Porta-Puglia, A. and Vannacci, G. 1989. Resistance of winter barley varieties subjected to nature of Pyrenophora graminea. J. Genet. Breed. 43:61-66.
20 Arru, L., Francia, E. and Pecchioni, N. 2003. Isolate-specific OTLs of resistance to leaf stripe (Pyrenophora graminea) in the Steptoe x Morex spring barley cross. Theor. Appl. Genet. 106:668-675.
21 Gatti, A., Rizza, F., Delogu, G., Terzi, V., Porta-Puglia, A. and Vannacci, G. 1992. Physiological and biochemical variability in a population of Drechslera graminea. J. Genet. Breed. 46:179-186.
22 Jawhar, M., Sangawn, R. S. and Arabi, M. I. E. 2000. Identification of Drechslera graminea isolates by cultural characters and RAPD analysis. Cereal Res. Comm. 28:89-93.
23 Hammouda, A. M. 1986. Modified technique for inoculation in leaf stripe of barley. Acta Phytopathol. Entomol. Hung. 21:255-259.
24 Kosambi, D. D. 1944. The estimation of map distances from recombination values. Ann. Eugen. 12:172-175.
25 Lander, E. S., Green, P., Abrahamson, J., Barlow, A., Daly, M. J., Lincoln, S. E. and Newburg, L. 1987. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174-181.   DOI
26 Aljanabi, S. M. and Martinez, I. 1997. Universal and rapid saltextraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res. 25:4692-4693.   DOI