• Transactions of Materials Processing
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• v.14 no.6 s.78
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• pp.570-576
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• 2005

Powder Injection Molding(PIM) has recently been recognized as an advanced manufacturing technology for low-cost mass production of metal or ceramic parts of complicated geometry With this regards, design technology of dental scaler tip PIM mold, which has complex shape and small core pin (diameter=0.6mm), with the help of computer-aided analysis of powder injection molding process was developed. Computer-aided analysis for dental scaler tip mold was implemented by finite element method with non-Newtonian fluid, modified Cross model viscosity, PvT data of powder/binder mixture. Compter-aided analysis results, such as filling pattern, weldline formation, air vent position prediction were compared with experimental result, and eventually have been shown good agreement. The core pin (diameter=0.6mm) deflection analysis of dental scaler tip PIM mold during PIM filling process was also investigated before mold fabrication.

• Parasites, Hosts and Diseases
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• v.49 no.1
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• pp.85-90
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• 2011

Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8$^+$ T-cells and CD4$^+$ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8$^+$ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.

• Journal of Ginseng Research
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• v.47 no.1
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• pp.123-132
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• 2023

Background: Pseudotyped virus systems that incorporate viral proteins have been widely employed for the rapid determination of the effectiveness and neutralizing activity of drug and vaccine candidates in biosafety level 2 facilities. We report an efficient method for producing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus with dual luciferase and fluorescent protein reporters. Moreover, using the established method, we also aimed to investigate whether Korean Red Ginseng (KRG), a valuable Korean herbal medicine, can attenuate infectivity of the pseudotyped virus. Methods: A pseudovirus of SARS-CoV-2 (SARS-2pv) was constructed and efficiently produced using lentivirus vector systems available in the public domain by the introduction of critical mutations in the cytoplasmic tail of the spike protein. KRG extract was dose-dependently treated to Calu-3 cells during SARS2-pv treatment to evaluate the protective activity against SARS-CoV-2. Results: The use of Calu-3 cells or the expression of angiotensin-converting enzyme 2 (ACE2) in HEK293T cells enabled SARS-2pv infection of host cells. Coexpression of transmembrane protease serine subtype 2 (TMPRSS2), which is the activator of spike protein, with ACE2 dramatically elevated luciferase activity, confirming the importance of the TMPRSS2-mediated pathway during SARS-CoV-2 entry. Our pseudovirus assay also revealed that KRG elicited resistance to SARS-CoV-2 infection in lung cells, suggesting its beneficial health effect. Conclusion: The method demonstrated the production of SARS-2pv for the analysis of vaccine or drug candidates. When KRG was assessed by the method, it protected host cells from coronavirus infection. Further studies will be followed for demonstrating this potential benefit.

• Journal of Korean Foot and Ankle Society
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• v.10 no.2
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• pp.140-143
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• 2006

Purpose: To present tibial axis-talar ratio and tibia-ankle surface angle in lateral measured on standing ankle lateral radiographs of adults who did not have specific ankle pathology. Materials and Methods: On Fifty-six radiographs without ankle osteoarthritis and malalignment, radiographic measures were performed with use of a custom dizitizing program based on $PV-WAVE^{(R)}$. AP ankle alignment was quantified by tibia axis-talar ratio (TTR) and tibia ankle surface angle in lateral (TLS angle). The data was compared with previously reported american data and analyzed using a t-test. Results: The average TTR and TLS angle of our series were $33.9{\pm}3.3%$, and no significant difference compared with reported american adults (p=0.152). Conclusion: The T-T ratio and TLS angle measured on standing ankle lateral radiographs of our series were $33.9{\pm}3.3%$ and $80.4{\pm}3.3^{\circ}$. This measures appear to be a useful measure for determining AP ankle alignment.

• Nuclear Engineering and Technology
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• v.53 no.7
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• pp.2407-2417
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• 2021

A structural integrity evaluation program (STEP) was developed for the high temperature reactor design evaluation according to the ASME Boiler and Pressure Vessel Code (ASME B&PV), Section III, Rules for Construction of Nuclear Facility Components, Division 5, High Temperature Reactors, Subsection HB. The program computerized HBB-3200 (the design by analysis procedures for primary stress intensities in high temperature services) and Appendix T (HBB-T) (the evaluation procedures for strain, creep and fatigue in high temperature services). For evaluation, the material properties and isochronous curves presented in Section II, Part D and HBB-T were computerized for the candidate materials for high temperature reactors. The program computerized the evaluation procedures and the constants for the weldment. The program can generate stress/temperature time histories of various loads and superimpose them for creep damage evaluation. The program increases the efficiency of high temperature reactor design and eliminates human errors due to hand calculations. Comparisons that verified the evaluation results that used the STEP and the direct calculations that used the Excel confirmed that the STEP can perform complex evaluations in an efficient and reliable way. In particular, fatigue and creep damage assessment results are provided to validate the operating conditions with multiple types of cycles.

• Solar Energy
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• v.6 no.2
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• pp.3-14
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• 1986

It is desirable to collect the solar thermal energy at relatively high temperature in order to minimize the size of thermal storage system and to enlarge the scope of solar thermal energy utilization. In this study, to develop a solar collector that has both advantages of collecting solar thermal energy at high temperature and fixing conveniently the collector system for long term period, a cylindrical parabolique concentrating solar collector (M.C.P.C.S.C) was designed, which has several rows of parabolique reflectors and thin thickness such as the flat-plate solar collector, maintaining the optical form of concentrating solar collector. The thermal performance of the M.C.P.C.S.C. newly designed in this study was analysed theoretically and experimentally. The results are summarized as follows: 1) prediction equation for outlet temperature, $T_o$, of heat transfer fluid and for the thermal efficiency, ${\eta}$, of the collector were derived as; o $$T_o=[C+B1_n(\frac{I_c(t)}{pv^3})]T_i$$ o $${\eta}=\frac{A}{A_c}\dot{m}[(C-1)+B1_n(E{\cdot}di^6\frac{I_c(t)}{\dot{m}^3})]\frac{T_i}{I_c(t)}$$ 2) When the insolation on the tilted solar collector surface, $I_c$, was $900-950W/m^2$ and the heat transfer fluid was not circulated in tubular absorber, the maximum temperature on the absorber surface was $100-118^{\circ}C$, this result suggested that the heat transfer fluid could be heated up to $98-116^{\circ}C$. The maximum temperature on the absorber surface was decreased with the increase of the collector shape factor, $L_p/L_w$ 3) There was a good agreement between the experimental and theoretical value of solar collector efficiency, ${\eta}$, which was proportional to the collector shape factor, $L_p/L_w$ 4) It is desirable to continue the study on the relationship between the collector shape factor, $L_p/L_w$, and the thermal efficiency of solar collector.

• Journal of IKEEE
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• v.22 no.3
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• pp.664-671
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• 2018

Generally, conventional solar tracking systems employ irradiance sensors to track a sun position, which enables the system to generate maximum solar energy. The usage of irradiance sensors increases system costs and deteriorates the performance of systems from sensor malfunctions. In this paper, a new solar tracking system without irradiance sensors has been proposed in which the controller capable of controlling and monitoring remotely is based on Artik platform. The proposed system tracks the sun position by comparing the amount of currents from several solar panels, resulting in removing irradiance sensors. In order to verify the performance of the proposed solar tracking method, the 12[V]-20[W] prototype system is built and implemented. Since the proposed system has remote monitoring functions through the employment of Artik as the IoT platform, more advantages in installation, maintenance and expanded functionality can be obtained compared to the conventional solar tracking system.

• The Transactions of the Korean Institute of Power Electronics
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• v.7 no.2
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• pp.171-177
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• 2002

The Photovoltaic systems with solar cell way Provide electrical energy to the utility/consumers, which are becoming one of the promising energy substitutes. The photovoltaic system can be classified into two types : One is the stand-alone type, and the other utility interactive one. The latter can return the generated power to the utility, but the former can't. The utility interactive systems are so valuable for peak power cut in summer season. In the photovoltaic systems the maximum power point tracking (MPPT) has been studied for the increase of the generating energy of the photovoltaic system. There are many control methods of MPPT, but a new MPPT algorithm is proposed to overcome the disadvantages of the conventional ones, and as a result the proposed method enables to improve both tracking ability and generating efficiency of photo voltaic system without DC chopper.

• The Plant Pathology Journal
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• v.34 no.3
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• pp.191-198
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• 2018

Fast and accurate diagnosis is needed to eradicate and manage economically important and invasive diseases like fire blight. Loop-mediated isothermal amplification (LAMP) is known as the best on-site diagnostic, because it is fast, highly specific to a target, and less sensitive to inhibitors in samples. In this study, LAMP assay that gives more consistent results for on-site diagnosis of fire blight than the previous developed LAMP assays was developed. Primers for new LAMP assay (named as DS-LAMP) were designed from a histidine-tRNA ligase gene (EAMY_RS32025) of E. amylovora CFBP1430 genome. The DS-LAMP amplified DNA (positive detection) only from genomic DNA of E. amylovora strains, not from either E. pyrifoliae (causing black shoot blight) or from Pseudomonas syringae pv. syringae (causing shoot blight on apple trees). The detection limit of DS-LAMP was 10 cells per LAMP reaction, equivalent to $10^4$ cells per ml of the sample extract. DS-LAMP successfully diagnosed the pathogens on four fire-blight infected apple and pear orchards. In addition, it could distinguish black shoot blight from fire blight. The $B{\ddot{u}}hlmann$-LAMP, developed previously for on-site diagnosis of fire blight, did not give consistent results for specificity to E. amylovora and on-site diagnosis; it gave positive reactions to three strains of E. pyrifoliae and two strains of P. syringae pv. syringae. It also, gave positive reactions to some healthy sample extracts. DS-LAMP, developed in this study, would give more accurate on-site diagnosis of fire blight, especially in the Republic of Korea, where fire blight and black shoot blight coexist.

• Journal of the Korean Solar Energy Society
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• v.30 no.6
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• pp.131-136
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• 2010

The purpose of this study is to provide foundational data for expansion of solar generation in building application, a clean energy, by introducing applicability of solar power generation system on roofs of industrial buildings and computing expected amounts of power and carbon dioxides reduction. As methodologies of this study, after reviewing 120,000 domestic factories to verify the BIPV feasibility for industrial building sthrough theoretical considerations of solar generation system, we calculated BIPV application methods and subsequent expected power generation quantity and carbon dioxide reductions through roof type analysis. we analyzed four cases of expected power generation amounts of solar batteries according to application methods, and when considering that the main type of roofs are slant roofs according to the investigation result about roof forms of domestic industrial complexes, we believe that the module angle of a slant roof around $17^{\circ}$(case3) is most suitable for the application. Finally, we came up with 517,944[TOE] as the corresponding petroleum tonnage based on this computed expected power generation amount and the amount of 1,214,836[$tCO_2$] carbon dioxide reductions by calculating them by energy sources.