• Journal of the Korean Magnetic Resonance Society
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• v.16 no.2
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• pp.147-161
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• 2012

Human melanocortin-4 receptor (hMC4R) among MC-Rs, expressed in the brain, is in charge of the control on energy homeostasis and food intake. The structure and function of human MC4R have been studied to understand their essential function and roles. To investigate the structure and function, it is necessary to prepare sufficient amounts of proteins. However, their expression and purification is demanding and time-consuming due to their innate insoluble and toxic properties. The heterozygous mutations of hMC4R, exchange of Asp 90 to Asn located in second transmembrane, cause severe obesity in human. To obtain purified hMC4R wt-TM2 for structural studies, it was first over-expressed and purified by fast protein liquid chromatography (FPLC) and then solution NMR studies were performed to get high-resolution spectra. In here, we established optimized purification scheme to get more purified target peptide.

• KCID journal
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• v.11 no.1
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• pp.36-44
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• 2004

The objective of this study is to analyze the characteristics of the nutrients loading and the water purification function in the paddy-fields. This study was carried out based on the research data in the three case studies. 1. Irrigation requirement is m

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.2
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• pp.179-185
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• 2006

The Ministry of Agriculture and Forestry announced in 2001 that the overall amount of paddy land set aside for rice will be cut down by 12% by 2005, decreasing from 1.08 million to 953,000 hectares. When evaluating the value of paddy rice systems, the multi-function of paddy systems in the monsoon climate is vital importance. The main functions of paddy rice systems are to conserve biodiversity and maintain sustainability. Some crucial environmental benefits of the paddy rice systems include: flood prevention, recharge of water resources, water purification, soil erosion and landslide prevention, soil purification, landscape preservation and air purification. The paddy rice systems in Korea, which are more diverse than upland crop systems, are known to be composed of 14 orders, 36 families and 134 species. The sustain ability of paddy rice production systems can never be overestimated. Rice is part of the culture and even the heart of spiritual life in the area under the monsoon climate. Therefore paddy rice systems should be preserved with the highest priority being the enhancement of the systems' multi-function. As an outlook to future research, the need of joint and interdisciplinary research projects between economists and natural scientists at inland as well as international levels were emphasized in establishing the development of counter-measure logic through actual proofed analysis.

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.277.1-277
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• 1998

No Abstract, See Full Text

• Journal of the Korean Applied Science and Technology
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• v.16 no.1
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• pp.75-81
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• 1999

HF purification performance of an ion exchange membrane(IEM) was evaluated with 0.5% HF spiked with 10ppb of Fe, Ni and Cu nitrates. The result show that after less than five turnovers through an IEM, the metallic impurity concentration drops below 1ppb. The decrease rate can be fitted to a model assuming the experimental tanks to be continuously stirred tank reaction and that the metallic impurity concentration after the IEM is a function of the single-pass purification efficiency of the membrane, the concentration before purification and the metals desorbed form the IEM. The Concentration after purification was investigated up to a cumulative Fe loading of 300ppb in the 23 liter recirculated loop. It increases linearly vs. cumulative loading and can be explained by the Langmuir theory resulting in a purification efficiency at the equilibrium of close to 99.5% in this loading regime.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.427-432
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• 2005

This paper is concerned with a simultaneous design of TSK (Takagi-Sugeno-Kang)-linguistic fuzzy models with uncertain model output and the computationally efficient representation. For this purpose, we use the fundamental idea of linguistic models introduced by Pedrycz and develop their comprehensive design framework. The design process consists of several main phases such as (a) the automatic generation of the linguistic contexts by probabilistic distribution using CDF (conditional density function) and PDF (probability density function) (b) performing context-based fuzzy clustering preserving homogeneity based on the concept of fuzzy granulation (c) augment of bias term to compensate bias error (d) combination of TSK and linguistic context in the consequent part. Finally, we contrast the performance of the enhanced models with other fuzzy models for automobile MPG predication data and coagulant dosing process in a water purification plant.

• Journal of Korean Society of Water and Wastewater
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• v.32 no.4
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• pp.309-315
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• 2018

Currently, the commercialization of the $5^{th}$ Generation (5G) service is becoming more prevalent in domestic communication network technology. This has reduced communication delay time and enabled large-capacity data transmission and video streaming services in real-time. In order to keep pace with these developments, K-water has introduced a smart process control system in water purification plants to monitor the status of the water purification process. However, since wireless networks are based on the public Long Term Evolution (LTE) network, communication delay time remains high, and high-resolution video services are limited. This is because communication networks still have a closed structure due to expense and security issues. Therefore, with 5G in its current form, it is very difficult to accommodate future services without improving the infrastructure of its communication networks. In recognition of these problems, this study implemented the authentication and management function of wireless networks on a wired network management system in the K-water Bansong water purification plant. The results confirmed that wired Local Area Network (LAN) services give a higher security performance than an expensive commercial wireless LAN system. This was achieved by using an Internet Protocol (IP) address management system of wired networks and the packet filtering function of the Layer2 (L2) switch. This study also confirmed that it is possible to create a wireless LAN service that is 3.7 times faster than the existing LTE communication network.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.12-17
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• 2006

Natural rivers have water purification functions called Gravel Contact Oxidation Process, which decontaminate river water by biological absorption, oxidation and degradation on riverbed gravels. This function has been developed and applied to many small/medium-sized urban rivers in Japan as one of the direct river water purification methods. However the method hasn't been verified in the tropics, which have different climate conditions and river characteristics. A preliminary experiment carried out at a polluted urban tributary in the outskirts of Kuala Lumpur, Malaysia where an increasing attention has been paid to river environment, showed a good applicability to the tropical conditions as a technically practical water purification measure with some maintenance cares for sludge management.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2004.11a
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• pp.92-92
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• 2004

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1808-1816
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• 2016

As a scaffolding subunit of the PIK3C3/VPS34 complex, Beclin 1 recruits a variety of proteins to class III phosphatidylinositol-3-kinase (VPS34), resulting in the formation of a distinct PIK3C3/VPS34 complex with a specific function. Therefore, the investigation of a number of Beclin 1 domains required for the protein-protein interactions will provide important clues to understand the PIK3C3/VPS34 complex, of which Beclin1-VPS34 interaction is the core unit. In the present study, we have designed a bacterial overexpression system for the Beclin 1 domain corresponding to VPS34 binding (Vps34-BD) and set up the denaturing purification protocol due to the massive aggregation of Vps34-BD in Escherichia coli. The expression and purification conditions determined in this study successfully provided soluble and functional Vps34-BD.