• Title/Summary/Keyword: Pure water

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Characterization and phylogenetic analysis of halophilic bacteria isolated from rhizosphere soils of coastal plants in Dokdo islands (독도 해안식물로부터 분리된 호염성 세균들의 특성 및 계통학적 분석)

  • You, Young-Hyun;Park, Jong Myong;Lee, Myung-Chul;Kim, Jong-Guk
    • Korean Journal of Microbiology
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    • v.51 no.1
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    • pp.86-95
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    • 2015
  • To study the halobacterial diversity at the rhizospheric soil of coastal plant native to Dokdo islands, several host plant were selected and its rhizospheric soil was sampled. Soil sample was diluted serially and pure isolation was done by sub-culture using marine agar media. 26 halophilic strains cultivable at the marine medium containig concentration of 9.0% sodium chloride were selected among total 161 isolates. Their partial 16S rRNA gene sequences extracted from genomic DNA were analyzed and partially identified. Furthermore, to identify their genetic relationship, phylogenetic tree was deduced. Total 26 strains were belongs to Firmicutes (30.8%), Gamma proteobacteria (53.8%), Bacteroidetes (7.7%), Alpha proteobacteria (7.7%), and Actinobacteria (7.7%). These results showed the specific difference from previous researches which has been reported the microbial flora of soil or sea water around the Dokdo islands. Furthermore, 4 among 26 halophilic strains grew at above 12.0% NaCl concentrated marine broth, and 2 strains Idiomarina abyssalis LM4H23 and Halomonas huangheensis AS4H13 grew at 15.0% concentration. These halophilic strains thought to overcoming the severe stress like high salt concentration or variation derived from Dokdo-specific climate and might have unknown, specific relationship with their host coastal plant native to Dokdo islands.

Effects of Anodic Voltages of Photcatalytic TiO2 and Doping in H2SO4 Solutions on the Photocatalytic Activity (광촉매 TiO2의 황산용액에서의 양극산화전압과 도핑이 광촉매 활성에 미치는 영향)

  • Lee, Seung-Hyun;Oh, Han-Jun;Chi, Choong-Soo
    • Korean Journal of Materials Research
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    • v.22 no.8
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    • pp.439-444
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    • 2012
  • To compare the photocatalytic performances of titania for purification of waste water according to applied voltages and doping, $TiO_2$ films were prepared in a 1.0 M $H_2SO_4$ solution containing $NH_4F$ at different anodic voltages. Chemical bonding states of F-N-codoped $TiO_2$ were analyzed using surface X-ray photoelectron spectroscopy (XPS). The photocatalytic activity of the co-doped $TiO_2$ films was analyzed by the degradation of aniline blue solution. Nanotubes were formed with thicknesses of 200-300 nm for the films anodized at 30 V, but porous morphology was generated with pores of 1-2 ${\mu}m$ for the $TiO_2$ anodized at 180 V. The phenomenon of spark discharge was initiated at about 98 V due to the breakdown of the oxide films in both solutions. XPS analysis revealed the spectra of F1s at 684.3 eV and N1s at 399.8 eV for the $TiO_2$ anodized in the $H_2SO_4-NH_4F$ solution at 180 V, suggesting the incorporation of F and N species during anodization. Dye removal rates for the pure $TiO_2$ anodized at 30 V and 180 V were found to be 14.0% and 38.9%, respectively, in the photocatalytic degradation test of the aniline blue solution for 200 min irradiation; the rates for the F-N-codoped $TiO_2$ anodized at 30 V and 180 V were found to be 21.2% and 65.6%, respectively. From the results of diffuse reflectance absorption spectroscopy (DRS), it was found that the absorption edge of the F-N-codoped $TiO_2$ films shifted toward the visible light region up to 412 nm, indicating that the photocatalytic activity of $TiO_2$ is improved by appropriate doping of F and N by the addition of $NH_4F$.

Analyses of Hydrology and Groundwater Level Fluctuation in Granite Aquifer with Tunnel Excavation (터널 굴착에 의한 화강암 대수층의 수리 수문 및 지하수위변동 분석)

  • Chung, Sang-Yong;Kim, Byung-Woo;Kang, Dong-Hwan;Shim, Byoung-Ohan;Cheong, Sang-Won
    • The Journal of Engineering Geology
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    • v.17 no.4
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    • pp.643-653
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    • 2007
  • Average hydraulic conductivity was $2.64{\times}10^{-8}m/sec$ average RQD was 78%, average porosity was 0.51%, and range of groundwater level was $77.06{\sim}125.97m$ by measured in 8 boreholes at the Surak Mt. tunnel area. Groundwater level of two peaks in the Surak Mt. tunnel area were estimated through linear regression analysis for groundwater level versus elevation. And, average horizontal hydraulic gradient in the Surak Mt. tunnel area was calculated 0.267. Minimum, maximum, and average hydraulic conductivities that estimated by field tests were $5.56{\times}10^{-9}m/sec,\;6.12{\times}10^{-8}m/sec,\;and\;2.64{\times}10^{-8}m/sec$, respectively. Groundwater discharge rates per 1 meter that estimated using minimum, maximum, and average hydraulic conductivities and average horizontal hydraulic gradient were $0.00585m^2/day,\;0.06434m^2/day,\;and\;0.02775m^2/day$, respectively. Pure groundwater recharge rate per unit recharge area was calculated 223.96 mm/yr through water balance analysis. Prediction simulation of groundwater level fluctuation with minimum, maximum, and average hydraulic conductivities were conducted. Discharge rate into the Surak Mt. tunnel for minimum hydraulic conductivity was small, but groundwaer drawdown was highly. Discharge rate into the Surak Mt. tunnel for maximum hydraulic conductivity was higher, but groundwaer level was recovered quickly.

Changes in taste Characteristics of Traditional Korean Soy Sauce with Ripening Period (I) - Analysis of General Compounds and Sugars - (숙성 기간에 따른 전통 간장의 맛 특성 변화(I) -일반 성분과 당류 분석-)

  • Joo, Myoung-Sook;Sohn, Kyung-Hee;Park, Hyun-Kyung
    • Journal of the Korean Society of Food Culture
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    • v.12 no.2
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    • pp.183-188
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    • 1997
  • This study was carried out in order to analyze the changes in general compounds and sugars of traditional Korean soy sauce with varying Meju concentrations (Meju-water ratios of 1 : 4 and 1.3 : 4) and ripening periods (up to 2 years). Along with ripening period, pH decreased and titratable acidity, total acidity and crude protein contents increased, which were higher in 1.3 : 4 than in 1.4. Buffering power increased significantly with increasing ripening period. Crude fat content was higher in 1.3 : 4 than 1 : 4. Moisture content decreased and pure extract content increased significantly with increasing ripening period, which was high in 1.3 : 4. Salt contents increased with increasing ripening period, but decreasd in soy sauce ripened for 2 years. As the result of sugar analysis, reducing sugar showed slow increase with increasing ripening period. Among detected free sugars, galactose was present in the highest concentration and the rest were in the sequence of glucose, fructose. Total free sugar content was highest in soy sauce ripened for 150days in 1 : 4 and 210 days in 1.3 : 4.

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A Study on the Electrochemical Properties of Porous Carbon Electrode according to the Organic Solvent Contents (유기용매의 함량비에 따른 다공성 탄소전극의 전기화학적 특성 연구)

  • Lim, Jung-Ae;Choi, Jae-Hwan
    • Applied Chemistry for Engineering
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    • v.19 no.2
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    • pp.185-190
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    • 2008
  • In order to increase the surface area of electrodes for electrosorption, porous carbon electrodes were fabricated by a wet phase inversion method. A carbon slurry consisting of a mixture of activated carbon powder (ACP), polyvinylidene fluoride (PVdF), and N-methyl-2-pyrrolidone (NMP) as a solvent was cast directly on a graphite sheet. The cast film was then immersed in pure water for phase inversion. The physical and electrochemical properties of the electrodes were investigated using scanning electron microscopy (SEM), porosimetry, and cyclic voltammetry. The SEM images verified that the pores of various sizes were formed uniformly on the electrode surface. The average pore sizes determined for the electrodes fabricated with various NMP contents ranged from 64.2 to 82.4 nm and the size increased as the NMP content increased. All of the voltammograms showed a typical behavior of charging and discharging characteristic at the electric double layer. The electrical capacitance ranged from 3.88 to $5.87F/cm^2$ depending on the NMP contents, and the electrical capacitance increased as the solvent content decreased. The experimental results showed that the solvent content is an important variable controlling pore size and ultimately the capacitance of the electrode.

Structure and physical properties of the earth crustal material in the middle of Korean Peninsula : A study on the prescription of standard test by mortar-bar method (한반도 중부권 지각물질의 구조와 물성연구 : 콘크리트 공시체에 의한 표준시험 규정에 대하여)

  • 정진곤;유신애
    • The Journal of Engineering Geology
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    • v.5 no.2
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    • pp.193-200
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    • 1995
  • It has been well known that the alkali-aggregate reaction between the aggregates and cement paste is one of the reasons of a concrete siructre expansion. Because of a serious demage on the concrete stnicture from the expansion, in many countries, the safety of the materials is checked in laboratory by mortar-bar test and the upper limit of expansion in length is 0.1%. The prescriptions are presented in the ASTM C227 and 490 of U.S. which has been international currency and in the KS Handbook F2503, F2546 and L5107 of Korea published by Korean Standards Association. Both of the prescriptions are almost same in their contents. Actually, in the process of preparing and measuring the mortar-bar according to the prescription mentioned above, it seems that there are no problems for its own purpose but a few points are found to be improved upon the methods to increase the accuracy for laboratory work as follows. 1. The prescription of blending ratio(aggregate, cement and water) should be noted by volume not by weight. 2. It is unreasonable to measure the initial length of mortar bars after 24$\pm$2 hours at once regardless the kind of aggregates. 3. It may bring about errors in calculating the expansion ratio under the condition of the denominator value fixed as 254mm. 4. The measuring methods of specific gravity are selected according to the purposes but the pure specific gravity displays the highest accuracy among them.

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Removal of H2S by Selective Catalytic Oxidation II. Selective Oxidation of H2S on TiO2/SiO2 Catalysts (선택적 촉매 산화 반응에 의한 황화 수소의 제거 II. TiO2/SiO2 촉매 상에서 황화 수소의 선택적 산화 반응)

  • Chun, S.W.;Park, D.W.;Woo, H.C.;Hong, S.S.;Chung, J.S.
    • Applied Chemistry for Engineering
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    • v.7 no.4
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    • pp.645-652
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    • 1996
  • Selective catalytic oxidation of $H_2S$ to elemental sulfur using $TiO_2/SiO_2$ catalysts was investigated in this study. The reaction test with pure $TiS_2$ and $Ti(SO_4)_2$ and cyclic temperature operation revealed that $TiO_2$ had a good resistance to sulfation and sulfidation, which are known as the main cause of catalytic deactivation in sulfur recovery process. With the increase of $TiO_2$ loading amount in $TiO_2/SiO_2$ catalysts, the conversion of $H_2S$ increased and the selectivity of elemental sulfur was very slightly decreased. As the ratio of $O_2/H_2S$ increased, the selectivity to elemental sulfur was drastically decreased. In the presence of 10 vol.% water vapor to a stoichiometric mixture of $H_2S$ and $O_2$($H_2S$= 5 vol.% O=2.5 vol.% ), both activity and selectivity of 10 wt.% $TiO_2/SiO_2$ catalyst are decreased, but it still showed more than 80% of sulfur yield.

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A Fundamental Study on the Auditory Characteristics of Amberjack Seriola dumerili in the Coast of Jeju Island (제주 연안산 잿방어의 청각특성에 관한 기초적 연구)

  • 서익조;김성호;김병엽;이창헌;서두옥
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.39 no.4
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    • pp.269-275
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    • 2003
  • In this paper, We examined auditory threshold and critical ratio of amberjack seriola dumerili, in the Jeju Island coastal waters, to find out hearing ability of the fish. The auditory threshold level, critical ratio and hearing index of amberjack were determinded by conditioning method using a sound coupled with electric shock in the condition of ambient noise or white noise in an experimental water tank. The audio-signals of pure tone and electric shock were from 80 HZ to 800 Hz and DC 7 V, respectively. Values for the critical ratios were calculated in terms of the masked thresholds using the noise projected to stable spectrum levels at all measurement frequencies of background noise. Masking noises were in the spectrum level range of 65 dB∼75 dB $(re 1{\mu}Pa\sqrt{Hz})$. The auditory thresholds of amberjack within the test the frequencies were most sensitive at 300HZ as 94.5 dB. The critical ratios of fishes ranged from 36.4 to 52.8 dB. The noise spectrum level that started masking was about 58∼72 dB within frequencies.

Taste Compounds of Fresh-Water Fishes 6. Taste Compounds of Korean Catfish Meat (담수어의 정미성분에 관한 연구 6. 메기의 정미성분)

  • YANG Syng-Taek;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.16 no.3
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    • pp.202-210
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    • 1983
  • In order to elucidate the taste compounds of Korean catfish, Parasilurus asotus, free amino acids, nucleotides and their related compounds, organic bases, sugars, organic acids and minerals in the extracts of Korean catfish were analyzed, and then evaluated by sensory test of synthetic extract prepared on the basis of the analytical data. Taste panel assessments of synthetic extracts prepared with each extractive component omitted were carried out by a triangle difference test, and changes in taste profile were assessed. In free amino acid composition, glycine was dominant occupying $25\%$ of total free amino acids. The other abundant free amino acids were lysine, taurine and alanine. Judging from the result of analysis of nucleotides, IMP was dominant showing about $70\%$ of total nucleotides while ATP, ADP, AMP, inosine and hypoxanthine were low in content. Among organic bases, total creatinine was abundant and its nitrogen content occupied more than $50\%$ of the total extractive nitrogen. The amount of betaine was 18mg/100g and trace amount of trimethylamine and trimethylamine oxide were detected. The main organic acids were succinic, butyric, propionic and valeric acid. On the other hand, oxalic, fumaric, maleic, tartaric and citric acids were analyzed in trace. As for the sugars, glucose was found to be the most abundant monosaccharide. Extremely small amounts of fructoae, inositol were also detected and ribose and arabinose were trace in content. $K^+,\;Na^+,\;PO_{4}^{3-}$ and $Cl^-$ were found to be the major ions and small amount of $Ca^{2+}$ were defected. The synthetic extract, prepared with about 40 pure chemicals based on the analytical data satisfactorily revealed the natural taste of the original extract except slight difference in meaty taste and mildness. From the results of omission test the major components which contribute to produce the taste were serine, IMP, succinic acid and $PO_{4}^{3-}$.

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EFFECTS OF TITANIUM SURFACE COATING ON CERAMIC ADHESION (타이타늄 표면 코팅이 도재 결합에 미치는 영향)

  • Kim, Yeon-Mi;Kim, Hyun-Seung;Lee, Kwang-Min;Lee, Doh-Jae;Oh, Gye-Jeong;Lim, Hyun-Pil;Seo, Yoon-Jung;Park, Sang-Won
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.5
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    • pp.601-610
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    • 2007
  • Statement of problem: The adhesion between titanium and ceramic is less optimal than conventional metal-ceramic bonding, due to reaction layer form on cast titanium surface during porcelain firing. Purpose: This study characterized the effect of titanium-ceramic adhesion after gold and TiN coating on cast and wrought titanium substrates. Material and method: Six groups of ASTM grade II commercially pure titanium and cast titanium specimens$(13mm{\times}13mm{\times}1mm)$ were prepared(n=8). The conventional Au-Pd-In alloy served as the control. All specimens were sandblasted with $110{\mu}m\;Al_2O_3$ particles and ultrasonically cleaned for 5min in deionized water and dried in air before porcelain firing. An ultra-low-fusing dental porcelain (Vita Titankeramik) was fused on titanium surfaces. Porcelain was debonded by a biaxial flexure test at a cross head speed of 0.25mm/min. The excellent titanium-ceramic adherence was exhibited by the presence of a dentin porcelain layer on the specimen surface after the biaxial flexure test. Area fraction of adherent porcelain (AFAP) was determined by SEM/EDS. Numerical results were statistically analyzed by one-way ANOVA and Student-Newman-Keuls test at ${\alpha}=0.05$. Results: The AFAP value of cast titanium was greatest in the group 2 with TiN coating, followed by group 1 with Au coating and the group 3 with $Al_2O_3$ sandblasting. Significant statistical difference was found between the group 1, 2 and the group 3 (p<.05). The AFAP value of wrought titanium was greatest in the group 5 with TiN coating, followed by the group 4 with Au coating and the group 6 with $Al_2O_3$ sandblasting. Conclusion: No significant difference was observed among the three groups (p>.05). The AFAP values of the cast titanium and the wrought titanium were similar. However the group treated with $Al_2O_3$ sandblasting showed significantly lower value (p<.05).