• International Journal of Industrial Entomology and Biomaterials
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• v.1 no.1
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• pp.25-28
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• 2000

A comprehensive study on daily pupal weight loss due to histolysis and histgenesis in Bombyx mori Linn., has been undertaken. The percentag of pupal weight loss in the male pupa is higher than the female, conforming that the female pupa require more energy in the form of less pupal weight loss for ovipositional activities. The regression equation clearly shows higher percentage loss of daily pupal weight in male than female with respect to age and, this is also evident from the slope of the regression line. Moreover, analysis of the coefficient of correlation shows that the weight loss of pupa is directly correlated with the age rather than climatic parameters under which the pupa dwells.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.1
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• pp.19-24
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• 2005

Nutrition is very essential for growth, development and reproduction. The quantitative impetus of nutritional factor on adult characters and reproductive fitness was studied in Antheraea mylitta by providing fresh leaves of Terminalia tomentosa for 1 to 4 times a day to $5^{th}$ instar larvae. All the characters have improved on giving fresh diet more times over the single diet. The adult weight has strong effect on the reproductive potential i.e., female pupa and moth weight, survivability, pupation rate, emergence percentage, fecundity, fertility, egg weight and hatched out larval weight. The improvement recorded to the tune of $70\%,\;77.1\%,\;115.4\%,\;36.7\%,\;45.9\%\;and\;218.3\%$ in larval weight gain, survivability, pupation rate, female pupa weight, moth weight and fecundity respectively when fresh diets were provided 4 times a day against single diet. The adult emergence percentage has increased from $66.4\%\;to\;96.2\%$ and fertility rose from $52.2\%\;to\;93.6\%$. The weight of eggs and the larvae hatched out of it also found to be significantly higher in 4 diets a day condition. The study revealed that optimization of fresh diet frequency should be maintained to obtain higher egg production and fertility in order to get vigorous larvae to continue the generation.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.1
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• pp.45-49
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• 2005

Respiration in Antheraea mylitta was studied using constant pressure respirometer. Oxygen uptake in different stages of life showed that moth has highest rate of oxygen uptake as compared to larva and pupa. At each stage significant sexual differentiation was observed. Depression in the rate of oxygen uptake in diapausing pupa was found to be higher in male ($65.75\%$) than female ($60.65\%$) as compared to non-diapausing counterparts. During diapause lowest oxygen uptake was recorded in the month of February, and were in the order of February < January < December < March < November < April < May < June in male, and, February < January < December < March < April < November < May < June in female. Significant sexual differentiation in oxygen uptake was recorded throughout the period of diapause (November to June) where female pupae registered lower rate of oxygen uptake than that of male. Oxygen uptake in female pupae declined upto $28.89\%$ in non-diapausing and $18.29\%$ in diapausing broods over male. Highest respiratory quotient value was recorded in the moth of November (0.68 in male and 0.70 in female) and, the lowest in the month of March in male (0.54) and May in female (0.55). Percentage loss in pupa weight always remained at a significantly higher level in male (except in February and March). The lowest oxygen uptake rate and weight loss in the month of February shows that the pupae were at peak of their dormancy during this month.

• Journal of Microbiology and Biotechnology
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• v.34 no.5
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• pp.1082-1091
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• 2024

The antioxidant capacity and protective effect of peptides from protein hydrolysate of Cordyceps militaris cultivated with tussah pupa (ECPs) on H₂O₂-injured HepG2 cells were studied. Results indicated ECP1 (<3 kDa) presented the strongest antioxidant activity compared with other molecular weight peptides. Pretreated with ECPs observably enhanced survival rates and reduced apoptosis rates of HepG2 cells. ECPs treatment decreased the ROS level, MDA content and increased CAT and GSH-Px activities of HepG2 cells. Besides, the morphologies of natural peptides from C. militaris cultivated with tussah pupa (NCP1) and ECP1 were observed by scanning electron microscopy (SEM). Characterization results suggested the structure of NCP1 was changed by enzymatic hydrolysis treatment. Most of hydrophobic and acidic amino acids contents (ACC) in ECP1 were also observably improved by enzymatic hydrolysis. In conclusion, low molecular weight peptides had potential value in the development of cosmetics and health food.

• International Journal of Industrial Entomology and Biomaterials
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• v.24 no.2
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• pp.63-68
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• 2012

Osteoporosis is characterized by the reduced density of mineralized bone matrix. The loss of bone has been attributed to an imbalance between bone formation and bone resorption. The silkmoth is one of the famous traditional tonic medicines. Silkworm pupa was evaluated for its antiosteoporotic activity in an ovariectomized rat model of osteoporosis. The rats were ovariectomized at 6weeks of age and divided into 7 groups. All the groups were fed without calcium diet. The OVX rats were treated with water and silkworm powder for 6 weeks. In OVX rats, the body weight, feeding and water intake quantity did not show a significant change, but the silkworm pupa powder immediately before the eclosion of Yeonrokjam was significantly increased the bone mineral density in the femoral bone. The silkworm pupa powder increased the bone with increasing calcium in serum. These results also showed its protective action through promotion of bone formation. The silkworm pupa powder has a definite antiosteoporotic effect; similar to estrogen, it is especially effective for the prevention of bone fracture induced by estrogen deficiency. The silkworm pupa powder is a promising anti-osteoporotic agent that can prevent bone fractures in osteoporotic patients. The silkworm pupa powder does not show serious side effects because natural herbal medicine origin.

• Mycobiology
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• v.38 no.2
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• pp.133-136
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• 2010

Stromatal fruiting bodies of Cordyceps cardinalis were successfully produced in cereals. Brown rice, German millet and standard millet produced the longest-length of stromata, followed by Chinese pearl barley, Indian millet, black rice and standard barley. Oatmeal produced the shortest-length of fruiting bodies. Supplementation of pupa and larva to the grains resulted in a slightly enhanced production of fruiting bodies; pupa showing better production than larva. 50~60 g of brown rice and 10~20 g of pupa mixed with 50~60 mL of water in 1,000 mL polypropylene (PP) bottle was found to be optimum for fruiting body production. Liquid inoculation of 15~20 mL per PP bottle produced best fruiting bodies. The optimal temperature for the formation of fruiting bodies was $25^{\circ}C$, under conditions of continuous light. Few fruiting bodies were produced under the condition of complete darkness, and the fresh weight was considerable low, compared to that of light condition.

• Korean journal of applied entomology
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• v.22 no.3 s.56
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• pp.228-231
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• 1983

In order to eradicate harmful insect by use of sterile male techniques, it is necessary to disperse about 10 times as many male insect as in wild condition. However, it is so difficult to discriminate male insect in pupal stage that usually the population are likely to be contaminated by female insect when that are released in field. The present study was conducted to establish a useful method capable of differenciating the male pupae of Stomawys calcitsans by means of measuring the pupal weight and length. The results were summarized as follows; 1. Male pupa was lighter and shorter than female and high correlation existed between length and width of pupa. 2. There was significant relationship between the length of pupa and width of adult 3. When the pupal sex of stable ay was identified by median of mean length and mean weight of the pupae, the Ratio of female to male was 1 : 1. 4 and 1 : 2. 2, respectively. Therefore, median of pupal weight seemed to be applicable for obtaining more number of male pupae.

• Korean Journal of Plant Resources
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• v.12 no.2
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• pp.102-106
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• 1999

This experiment was carried out to find the method for mass production by artifical cultivition and to analyze the components of Paecilomyces japonica according to several media. Time of inoculation of the Paecilomyces japonica using silkworm was on first day of five molting and infection rate was 72.0%. Optium medium for mass production of the Paecilomyces japonica was known effective for increasing dry weight and fruitbody at brown rice 80g plus pupa powder 20g. Dry weight of Paecilomyces japonica using fungus of silkworm was 1.2g including pupa and length of fruitbody was appeared 3.0cm to 3.5cm. Content of $\beta$ - glucan was very high as 40.5% at inoculation on the first day of the five molting while 16.4% at brown rice, 20.7% at pupa, 23.1% at brown rice plus pupa powder, and 28.7% at pine sawdust plus wheat bran. Mycelium was poor and pinkly conidiospore was formed on media of centipede, maggot and powder of silkworm.

• International Journal of Industrial Entomology and Biomaterials
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• v.20 no.2
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• pp.75-77
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• 2010

Until now, the main treatment for impotence has been the vasodilator injection, penile implants and etc. Among many methods, most effective way is known to be the medical therapy. The oral drug for an impotence remedy has to be above all, effective to the erection, and second, safety when taking in long-term. However, so far the drug in such condition and sufficiency has not been discovered. Consequently, it is crucial to develop the new medicine, made of natural materials only, without any uncertainty of side effect. The silkworm is very difference in physiological chemical change according to growth stage. Therefore the functional effect shows a very big difference according to preparation method with a growth stage. This research carry out the experiment with the pupa powder immediately before the eclosion, eclosion adult, the pupa extract immediately before the eclosion, and the silkworm powder of 5 instar 3rd day. The result showed not a big chance in weight, feed and water intake quantity. But testosterone hormone secretion effects of the freeze drying powder of the pupa immediately before the eclosion was very high 7.31 ng/ml, compared to 2.37 ng/ml of non treatment and 2.67 ng/ml of alcohol extraction method. As this result, the freeze drying pupa powder of the pupa immediately before the eclosion was confirmed to promote the secretion of the testosterone hormone more than alcohol extraction method of existing.

• Journal of Life Science
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• v.21 no.11
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• pp.1541-1548
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• 2011

This study was carried out to evaluate the effects of the addition of different levels of CFP (Cordyceps with fly pupa) on growth performance in broiler chickens. 400 broiler chicks (Ross 308, 1 day old) were sorted randomly into 4 treatment groups and fed experimental diets for 35 days. The treatment groups were divided into a control group not fed with CFP (T1), and treatment groups fed with CFP 2.0% (T2), CFP 3.5% (T3), and CFP 5.0% (T4). Although the broilers' weight gain and feed efficiency were significantly higher (p<0.05) in the T3 group throughout the entirety of the test period, no statistically significant differences were noted among the T1 and T2, T4 groups. Triglyceride in the blood, total cholesterol, and LDL-C were significantly lower in the CFP treatment groups than in the control group (p<0.05). The blood lipid reduction rate ranged from 5.32 to 10.63% for triglycerides, from 9.23 to 12.62% for total cholesterol, and from 44.67 to 53.81% for LDL-C in the CFP treatment groups relative to the control group. The abdominal fat weight ratio was reduced significantly in the CFP treatment groups (p<0.05) compared with the control group, with a reduction rate range of 17.67-21.68%. Broiler carcass weight, carcass rate, and ratios of breast muscle, skin and thigh muscle weights to carcass weight were significantly higher in the T3 group, and statistically significant differences were noted among the T1 and T2, T4 groups (p<0.05). Enteropathogenic E.coli and Salmonella were lower in the CFP treatment groups than in the control group, whereas the beneficial bacteria Bifidobacteria were significantly higher in the CFP treatment groups than in the control group (p<0.05). These findings suggest that the Cordyceps with fly pupa can improve the carcass characteristics and body weight gain in broiler chickens.