• 사상체질의학회지
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• 제28권2호
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• pp.147-162
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• 2016

Objectives The objective of this study is to develop a taeeumin animal-experimental model induced lung fibrosis with Bleomycin and evaluate the effect on obesity in this animal-experimental model.Methods The subjects were divided into 3 groups : normal group, high fat diet(HFD) control group, and HFD group administered with bleomycin(n=10 per group). To develop taeeumin animal-experimental model with reduced respiratory metabolism, 8-week-old C57BL/6 mice were administered with 0.03ml solution of bleomycin 1U/ml dissolved in distilled water, intratracheal(IT), once. Then, the HFD control group and the experimental group were fed with high fat diet for 6 weeks. Airway hyperresponsiveness(AHR) to methacholine was measured at the 1st and 3rd week after bleomycin was administered. Food intake and body weight were measured at regular time weekly. After the final experiment, blood was gathered by cardiac puncture for bloodchemical examination and organs(liver, fatty tissue) were remoed, weighted, and mRNA was analyzed.Results and Conclusions Through the experiment, it was found that Bleomycin induced Taeeumin animal-experimental models have leptin resistace. In the experimental group administered with Bleomycin, fatty acid synthesizing gene expression increased and energy metabolizing gene expression decreased. As mRNA expression of adiponectin decreased, it was found that Taeeuim animal-experimental model is susceptible to metabolic syndrome and cardiovascular diseases.

• 동서간호학연구지
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• 제6권1호
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• pp.37-45
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• 2001

The purpose of this study was to identify the effect of hand acupuncture for reduction of dysmenorrhea. 71 female middle school and high school students were selected as subjects from April to May 2001, among which 38 students consisted of the experimental group while 22 students were the control group by convenient assignment. The experimental group participated in hand acupuncture treatment. The experimental treatment was to puncture corresponding points(A4, A5, J1, J3, J23, H2, F7) on both the palm and back of a hand with disposable acupuncture needles and to remove needles after 20-30 minutes' recess during which the subjects reposed themselves on bed. The collected data were processed using the SPSS PC Program and analyzed using $X^2test$ and The results of this study were as follows 1. The symptoms of premenstrual syndrome have decreased, but no significant differences were statistically observed between hand-acupunctured group and non-hand acupunctured group. 2. Subjective pain degree has decreased after hand acupuncture. 3. As for objective pain degree, a significant reduction in voice change was observed between two groups. Facial expressional changes and perspiration degree reduced but showed no significant statistically changes between two groups. 4. Comfort has increased after hand acupuncture. These results suggest that the hand acupuncture therapy works effectively on easing menstrual pain and symptoms as well as partially on reducing discomfort. It is particularly efficacious for a reduction in subjective pain and facial expressional changes in positive respect. Therefore the hand acupuncture therapy can be considered as an independent nursing intervention for dysmenorrhea reduction.

• Annals of Clinical Neurophysiology
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• 제5권2호
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• pp.171-176
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• 2003

Backgrounds and Objectives: Despite of enormous clinical and laboratory researches focused on the useful markers in status epilepticus(SE), clinically applicable methods are not yet available. Although ketogenic diet (KD) is an old method of treating epilepsies, its outstanding antiepileptic effect in some epileptic patients needs re-evaluation of this methods. This study was performed to evaluate the effect of KD on the change of nitric oxide(NO) during the SE. Methods: After the determination of critical EEG stages in the pilocarpine-induced SE model, serum NO levels were measured with Griess reaction. Open cardiac puncture was done immediately after the four different EEG stages of SE in the KD rats and regular diet (RD) rats. Cessation of SE was done with the 10~20 mg/Kg of diazepam i.p. injection in each stages of SE in KD and RD rats. Results: Pilocarpine-induced SE showed reliable EEG and behavioral patterns in all rats. Also, KD did not affect the SE induced by pilocarpine in terms of the SE induction time and SE severity. Serum NO was consistently higher in KD rats than RD rats in all SE stages. Conclusions: KD significantly increases NO during the pilocarpine-induced SE. These finding might contribute the neuroprotective effect of KD in the SE.

• 한국간호교육학회지
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• 제12권2호
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• pp.265-271
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• 2006

Purpose: To analyze the newly developed vacuum assisted auto-lancing technique applied to the forearm for the purpose of obtaining an adequate blood sample for glucose test with minimal pain. Methods: Visual and facial pain measures were introduced to compare lancing pain between the forearm and fingertip in 58 normal females. Sampled blood volumes were accurately measured by computer scanning technique. Results: Visual pain measure demonstrated significant pain reduction effect of the forearm sampling compared with the traditional fingertip sampling, which was also consistent with facial pain measure results. Blood volume more than $0.5{\mu}L$, enough for blood glucose testing with modern glucometers, was collected in 399(86%) of 464 forearm samples. Conclusions: Capillary blood sampling could be performed with minimal pain on the forearm by the newly developed vacuum assisted auto-lancing technique. With some resampling when necessary, the forearm sampling seemed very useful, considering almost no pain felt by the patients.

• Clinical and Experimental Reproductive Medicine
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• 제40권4호
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• pp.155-162
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• 2013

Objective: Stress is known to be an inhibitor of the reproductive hypothalamic-pituitary-gonadal (HPG) axis. However, the neural and molecular connections between stress and reproduction are not yet understood. It is well established that in both humans and rodents, kisspeptin (encoded by the kiss1 gene) is a strong stimulator of the HPG axis. In the present study we hypothesized that endocannabinoids, an important neuromodulatory system in the brain, can act on the HPG axis at the level of kiss1 expression to inhibit reproductive function under stress. Methods: Adult male Wistar rats were unilaterally implanted with an intracerebroventricular cannula. Afterwards, the animals were exposed to immobilization stress, with or without the presence of the cannabinoid CB1 receptor antagonist AM251 (1 ${\mu}g/rat$). Blood samples were collected through a retro-orbital plexus puncture before and after stress. Five hours after the stress, brain tissue was collected for reverse transcriptase-quantitative polymerase chain reaction measurements of kiss1 mRNA. Results: Immobilization stress (1 hour) resulted in a decrease in the serum luteinizing hormone concentration. Additionally, kiss1 gene expression was decreased in key hypothalamic nuclei that regulate gonadotrophin secretion, the medial preoptic area (mPOA), and to some extent the arcuate nucleus (ARC). A single central administration of AM251 was effective in blocking these inhibitory responses. Conclusion: These findings suggest that endocannabinoids mediate, at least in part, immobilization stress-induced inhibition of the reproductive system. Our data suggest that the connection between immobilization stress and the HPG axis is kiss1 expression in the mPOA rather than the ARC.

• 한방안이비인후피부과학회지
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• 제22권1호
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• pp.62-75
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• 2009

Objective : The purpose of this study is to examine the effects of Gamisipjeontang-gamibang(GT) on an experimental bum elicitation. Method : The absorbance of the photo cell mixed with GT at the Abs 560 nm was measured after irradiation for 1 min. In order to know the antioxidant effect on skin cell of mice after burn elicitation, superoxide dismutase(SOD) activity was measured. Also, in order to know effects on the skin regeneration in the burned mice, we counted the eosinophil in blood from animals via cardiac puncture and observed the histological structure in the epidermal basal layer and the dermal section on the 3rd, 7th and 14th day after burn elicitation. We also studied changes in angiogenesis in the capillary surrounding the basal layer and dermal papilla. The changes of HSP70 distribution and changes of p53 positive reaction were observed to investigate the changes of the stress in the skin as well. Result : The results indicated that GT has a significant impact on the antioxidant effect on skin cells of mice after bum elicitation by increasing SOD activity in vitro test. GT seems to decrease MIP-2 which induces neutrophil infiltration and promotes the angiogenesis dose-dependently. Furthermore, GT decreased HSP70, the expression of which was induced by elevated temperatures, and p53 which induce a apoptosis after stress. Conclusion : GT can be applied to burned skin through its antioxidant effect and skin regeneration property.

• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1983년도 제17차 학술대회연제순서 및 초록
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• pp.18.3-19
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• 1983

후비공 폐쇄는 협비막의 과열부전, 협인두막의 지속적 잔존, Olfactory pit의 발생시 불완전 등의 선천성 원인과, 매독디프테리아, 수술 후 또는 외상 등으로 오는 후천성으로 나눌 수 있으며 선천성인 경우에는 두부, 심장, 소화기 계통에 여러 가지 기형이 동반되기도 한다. Johann Roedere (1755년)은 이 질환의 예를 최초로 보고하였으며 Emmert (1853년)가 골성 폐쇄를 트로카를 이용하여 처음 수술을 시도한 이래 , 비강내, 구개, 상악동, 그리고 비중격을 통한 수술 방법이 제시되어 왔으나 이중 경주개 경유법이 술후 재협착 빈도가 가장 적고 또 수술시 폐쇄부의 확인이 용이하여 가장 널리 이용되고 있다. 저자들은 선천성 양측 부분적 골부 후비공 폐쇄를 보인 11세 남아와 선친성 우측 완전 골막부 후비공 폐쇄와 좌측 부분적 골막부 후비공 폐쇄와 좌측 부분적 골막부 후비공 폐쇄를 보인 9세 여아에서 경구개 경유법으로 치험하였기에 문헌적 고찰과 함께 보고하는 바이다.

• Korean Journal of Acupuncture
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• 제33권2호
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• pp.75-83
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• 2016

Objectives : A previous study demonstrated that the connecting points of three yang meridians attenuated changes of nNOS, and Norepinephrine(NE) in rats. The current study investigated the changes in nNOS, NO and NE upon the needle insertion at varying depths at the connecting point of three yin meridians of the hand. Methods : Needles were inserted into rats, on both left and right sides of the connecting point, including the LU7, HT5 and PC6 acupoints which are three yin meridians of the hand. After insertion, needles were retained for five minutes. Each acupuncture groups were treated acupuncture at each acupoint and at the depths of superficial, middle and deep layer. After the retention, blood was drawn via cardiac puncture, and tissues of each point near meridian vessel was extracted to examine the changes in the expression of nNOS, NO and NE. Results : Compared with the superficial layer group, nNOS production significantly decreased in the middle and deep layer at LU7 acupoint group and in the deep layer at HT5, PC6 acupoint group. The tissue NE decreased in the deep layer on PC6 acupoint and the plasma NE increased at the middle layer at LU7 acupoint group but decreased at the deep layer on at LU7 acupoint group. Conclusions : Acupuncture at connecting points of three yin meridians of the hand can regulate the activities of nNOS, and NE.

• The Korean Journal of Physiology
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• 제14권2호
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• pp.1-5
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• 1980

This study was undertaken to investigate the influence of prostaglandin $E_1(PGE_1)$ upon the activity of carbonic anhydrase and upon the inhibitory action of acetazolamide on carbonic anhydrase. The heparinized blood was sampled by cardiac puncture from Sprague-Dawley strain rats under ether anesthesia and was hemolysed by adding distilled water 1,000 times the amount of the blood. The activity of carbonic anhydrase of 0.1 ml of the hemolysate was measured by Maren's simplified micro-method. In the first experiment, the 7 rats were used, and the activity was measured by adding 0.1 ml of various concentrations of $PGE_1$(0.5, 1.25, 2.5, 5.0, 10 and $20\;{\mu}g/ml$). In the second experiment, the 6 rats were used and the activity was measured by adding 0.1 ml of $PGE_1(5\;{\mu}g/ml)$ and 0.1 ml of acetazolamide$(6{\times}10^{-7}M/l)$ respectively or simultaneously. Obtained results were as follows: 1) The activity of carbonic anhydrase was significantly inhibited by $PGE_1$ at doses of $0.5{\sim}10\;{\mu}g/ml$, maximally at a dose of $2.5\;{\mu}g/ml$, but inhibition was no more observed at a dose of $20\;{\mu}g/ml$. 2) The activity of the acetazolamide group was significantly less than that of the control group. 3) The activity of the $PGE_1+acetazolamide$ group was significantly less than those of the $PGE_1$ group and the acetazolamide group. It is inferred from the above results that the $PGE_1$ inhibits the activity of carbonic anhydrase dose-dependently and strengthens the inhibitory effect of acetazolamide on carbonic anhydrase.

• 미생물학회지
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• 제5권2호
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• pp.97-101
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• 1967

Up to the present time, there are only three methods by which we can obtain bacteria free crown gall tissue. According to some papers related to this field, the first method is based on the works of Braun(53') who maintained infected plants at 46-47'c for several days. But the method has a problem that very few plants can tolerate this temperature. The second method is based on the well known observation that old tumors appear to be bacteria free at least 1 or 2% of the explants. Also this method is known to us as laborious and time consuming. The third method is the one we were using that was attempting to kill the bacteria with bacteriacidal agent such as Antibiotics. In fact., it is reported that almost complete control of crown gall of tomato was obtained by Blanchard('51) when plants were grown in a nutrient containing Aureomycin(20${\mu}g$/ml) following needle puncture with the gall bacteria. We have been engaged in making the experiment by applying solution of Penicillin, Streptomycin and of Chloramphenicol(Succinate free) to find the strong bacteriacidal agent through the method of disc plate, and to confirm the effect of antimicrobial action through the method of plant tissue culture system without possible injury to the host plant. The result of this report is the fact the strongest bacteriacidal agent among the above three Antibiotics was Chloramphenicol(Succinate free 1000 p.p.m). and that there happened no injury to the tissue cultures in a White's 10X media containing 1000 p.p.m. of Chloramphenicol.