• Journal of the Korean institute of surface engineering
• /
• v.30 no.5
• /
• pp.298-309
• /
• 1997

Hard Tin coating on tool steel substrate were prepared using the pulsed PACVE. An orthogonal experimental design was employed to find the best deposition conditions for TiN coating and to systematically understand the effect of processing parameters. The small size Taguchi matrix called the L16 was used for experiment and ANOVA(ANalysis of VAriance) was followed to study the effect of main parameters on hardness and adhesion TiN coatings. In conclusion, pulse on/off time ratio and pulsing frequency were the major deposition parameters to determine hardness and adhesion of TiN coating in the pulsed DC PACVE process. (200) preferred orientation, columnar growth and dome-shaped surface morphology of the TiN films gave rise to a high hardness and a good adhesion to the substrates.

• IMMUNE NETWORK
• /
• v.5 no.3
• /
• pp.163-171
• /
• 2005

Background: To perform the successful dendritic cell-based cancer immunotherapy one of the main issues to be solved is the source of antigen for DC pulsing. Limitations occur by using auto-tumor lysate due to the difficulties obtaining enough tumor tissue(s) quantitatively as well as qualitatively. In this study the possibility of allogeneic tumor cell lysate as a DC pulsing antigen has been tested in mouse melanoma pulmonary me tastasis model. Methods: B16F10 melanoma cells $(1{\timeS}10^5/mouse)$ were inoculated intra venously into the C57BL/6 mouse. Therapeutic DCs were cultured from the bone marrow myeloid lineage cells with GM-CSF and IL-4 (1,000 U/ml each) for 7 days and pulsed with lysate of either autologous B16F10 (B-DC), allogeneic K1735 (C3H/He origin; K-DC) or CloneM3 (DBA2 origin; C-DC) melanoma cells for 18 hrs. Pulsed-DCs $(1{\times}10^6/mouse)_{[CGP1]}$ were injected i.p. twice with one week interval starting from the day 1 after tumor cell inoculation. Results: Without observable toxicity, allogeneic tumor cell lysate pulsed-DC induced the significantly better anti-tumor response (tumor scale: $2.7{\pm}0.3,\;0.7{\pm}0.3\;and\;0.3{\pm}0.2$ for saline, B-DC and C-DC treated group, respectively). Along with increased tumor specific lymphocyte proliferations, induction of IFN-${\gamma}$ secretion against both auto- and allo-tumor cell lysates was observed from the DC treated mice. (w/B16F10-lysate: $44.97{\pm}10.31,\;1787.94{\pm}131.18,\;1257.15{\pm}48.27$, w/CloneM3 lysate: 0, $1591.13{\pm}1.83,\;1460.47{\pm}86.05pg/ml$ for saline, B-DC and C-DC treated group, respectively) Natural killer cell activity was also increased in the mice treated with tumor cell lysate pulsed-DC ($8.9{\pm}_{[CGP2]}0.1,\;11.6{\pm}0.8\;and\;12.6{\pm}0.7%$ specific NK activity for saline, B-DC and C-DC treated group, respectively). Conclusion: Conclusively, promising data were obtained that allogeneic-tumor cell lysate can be used as a tumor antigen for DC-based cancer immunotherapy.

• Proceedings of the KIPE Conference
• /
• 2003.07a
• /
• pp.264-268
• /
• 2003

This paper describes a high voltage high power DC power supply which has the ability of pulsed mode operation. The power supply Is constructed with several series connected power converters based on modified multilevel converters. The modified multilevel converters are suitable for the protection of frequent output short-circuit. The output dc power of the proposed converter can be disconnected from the load within several hundred microseconds at the instant of short-circuit fault. The rising time of the dc load voltage is as small as several hundred microseconds, and there is no overshoot of the do voltage because the dc output capacitors keep undischarged state. Analysis, simulations, and experiments are carried out to Investigate the operation and usefulness of the proposed scheme.

• IMMUNE NETWORK
• /
• v.5 no.1
• /
• pp.36-44
• /
• 2005

Background: The anti-tumor therapeutic effect of autologous tumor cell lysate pulseddendritic cells (DCs) was studied for non-immunogenic and immune suppressive lung cancer model. To test the possibility as an adjuvant therapy, minimal residual disease model was considered in mouse in vivo experiments. Methods: Syngeneic 3LL lung cancer cells were inoculated intravenously into the C57BL/6 mouse. Autologous tumor cell (3LL) or allogeneic leukemia cell (WEHI-3) lysate pulsed-DCs were injected twice in two weeks. Intraperitoneal DC injection was started one day (MRD model) after tumor cell inoculation. Two weeks after the final DC injection, tumor formation in the lung and the tumor-specific systemic immunity were observed. Tumor-specific lymphocyte proliferation and the IFN-${\gamma}$ secretion were analyzed for the immune monitoring. Therapeutic DCs were cultured from the bone marrow myeloid lineage cells with GM-CSF and IL-4 for 7 days and pulsed with tumor cell lysate for 18 hrs. Results: Compared to the saline treated group, tumor formation was suppressed in 3LL tumor cell lysate pulsed-DC treated group, while 3LL-specific immune stimulation was minimum. WEHI-3-specific immune stimulation occurred in WEHI-3 lysate-pulsed DC treated group, which had no correlation with tumor regression. Conclusion: The data suggest the possible anti-tumor effect of cultured DCs as an adjuvant therapy for minimal residual disease state of lung cancer. The significance of immune modulation in DC therapy including the possible involvement of NK cell as well as antigen-specific cytotoxic T cell activity induction was discussed.

• Journal of Welding and Joining
• /
• v.25 no.6
• /
• pp.71-77
• /
• 2007

Until now, many researches on laser-arc hybrid welding processes have been conducted mainly for high power CW laser and high direct current arc to weld the thick steel plates for shipbuilding. Recently, however the usage of thin steel plates, which tend to be deformed easily by thermal energy, is been increasing because of demand of light structure such as car body in the automobile industry. Accordingly, heat sources having relatively low heat input such as pulsed laser, dip-transfer DC GMA and pulsed GMA seem to be applied more increasingly and the study about those heat sources is needed more intensively. Any heat source mentioned above can not stand alone without weld defects at a relatively high welding speed for increasing the welding productivity. This is main reason to apply the hybrid welding process which uses pulsed laser and low-heat-input GMA heat sources simultaneously to weld the thin steel plate. In this study, parameters of pulsed laser and dip-transfer DC GMA welding are studied firstly through preliminary experiments, and then analyzed in the viewpoint of their physical phenomena. Before conducting the hybrid welding, a pulse control technique is developed based on the parallel port communication and Visual C++ 6.0. Owing to development of this technique, interactions of laser and arc pulses can be controlled consistently. Using the pulse control technique, the hybrid welding is conducted and then its interactive welding phenomenon is analyzed.

• Proceedings of the Korean Institute of Surface Engineering Conference
• /
• 2009.10a
• /
• pp.127-127
• /
• 2009

고밀도 유도 결합 플라즈마와 함께 -1 kV의 높은 펄스 직류 바이어스를 이용한 플라즈마 공정 장치를 CFD-ACE+를 이용하여 시변 모델로 해석하였다. 수 kHz의 낮은 펄스주파수와 $5{\mu}s$의 빠른 펄스 상승 시간의 효과에 의한 플라즈마 특성을 모사한 결과 전자 온도의 상승과 그에 의한 플라즈마 가열은 펄스 상승 시간 보다 수 ${\mu}s$늦게 발생하였으며 쉬스의 팽창은 Ar 10 mTorr에서 약 20 mm정도이며 계산된 전자 온도는 최고 20 eV에 이른다.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.22 no.3
• /
• pp.380-388
• /
• 2011

In this paper, we developed the X-band 63 watt pulsed SSPA(Solid State Power Amplifier) by using HMIC(Hybrid Microwave Integrated Circuits). The pulsed SSPA consists of power supply and 3-stage amplifier modules : pre-amplifier stage, driver-amplifier stage, final-amplifier stage. The developed pulsed SSPA provides more than 63 watts of output power with a short pulse width and the duty cycle of up to 1.2 % at $70^{\circ}C$. The fabricated module offers great than 37 dB of saturated gain across the operating band. Input and output VSWR is <1.5:1. This module has an average current of 400 mA typical and operates at a +28 $V_{dc}$ supply. The developed SSPA in this paper can apply to pulsed Doppler radar with high speed operation.

• The Transactions of the Korean Institute of Electrical Engineers B
• /
• v.53 no.1
• /
• pp.47-54
• /
• 2004

A new high voltage DC power supply is proposed. The proposed power supply is constructed with several power converters connected in series. It is easy to obtain high DC voltage for the same structure of each power converter. The output DC power of the proposed power supply can be disconnected from the load within several hundred microseconds at the instant of a load short-circuit fault. The rising time of the output DC voltage is also as small as several hundred microseconds, and there is no overshoot of the voltage because all of the output filter capacitors keep undischarged state even in load short-circuit condition. Therefore, the proposed scheme is suitable for the protection of frequent output short-circuit and fast on/off switching of output DC voltage. The proposed power supply has improved features such as simple structure, high power factor, and reduced size and volume compared with the conventional schemes. The operating principle is described and the validity of the proposed scheme is proved through simulations and experiments.

• Proceedings of the Korean Institute of Surface Engineering Conference
• /
• 1998.11a
• /
• pp.86-87
• /
• 1998

• IMMUNE NETWORK
• /
• v.5 no.3
• /
• pp.172-178
• /
• 2005

Background: Carcinoembryonic antigen (CEA) is well-known soluble tumor marker frequently detectable in peripheral blood of carcinoma patients and considered as good target for antigen-specific immunotherapy. However, it is known that the induction of immune response to CEA is very difficult because CEA is a self-antigen expressed in fetal cells and weakly expressed in normal colorectal epithelial cells. To enhance anti-tumor immunity specific for CEA, recombinant CEA protein was modified using listeriolysin O (LLO) for endosomal lysis and trans activator of transcription (Tat) domain for transducing extracellular proteins into cytoplasm. Methods: After immunization using dendritic cells pulsed with Tat-CEA, both Tat-CEA and LLO, and both Tat-CEA and Tat-LLO, antibody titer to CEA and LLO, cytotoxic T lymphocyte activity and the frequency of IFN-${\gamma}$ producing T lymphocytes were measured. Results: Immunization using DC pulsed with both Tat-CEA and Tat-LLO protein showed the increasement of production of CEA-specific antibody in serum, cytotoxic T lymphocyte activity, the frequency of IFN-${\gamma}$ secreting T cells, compared with DC pulsed with both Tat-CEA and LLO. Furthermore the ratio of CD8+T cell to $CD4^+$ cell among CEA-specific T cells was increased in group pulsed with both Tat-CEA and Tat-LLO. Conclusion: These results suggested that DC vaccine using Tat-LLO could be used for the development of effective immunotherapy for the treatment of tumor.