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Immunocell Therapy for Lung Cancer: Dendritic Cell Based Adjuvant Therapy in Mouse Lung Cancer Model  

Lee, Seog-Jae (Department of Thoracic & Cardiovascular Surgery, Eulji University School of Medicine)
Kim, Myung-Joo (The Cancer Center, Samsung Medical Center, Sungkyunkwan University School of Medicine)
In, So-Hee (The Cancer Center, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Baek, So-Young (The Cancer Center, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Lee, Hyun-Ah (The Cancer Center, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Publication Information
IMMUNE NETWORK / v.5, no.1, 2005 , pp. 36-44 More about this Journal
Abstract
Background: The anti-tumor therapeutic effect of autologous tumor cell lysate pulseddendritic cells (DCs) was studied for non-immunogenic and immune suppressive lung cancer model. To test the possibility as an adjuvant therapy, minimal residual disease model was considered in mouse in vivo experiments. Methods: Syngeneic 3LL lung cancer cells were inoculated intravenously into the C57BL/6 mouse. Autologous tumor cell (3LL) or allogeneic leukemia cell (WEHI-3) lysate pulsed-DCs were injected twice in two weeks. Intraperitoneal DC injection was started one day (MRD model) after tumor cell inoculation. Two weeks after the final DC injection, tumor formation in the lung and the tumor-specific systemic immunity were observed. Tumor-specific lymphocyte proliferation and the IFN-${\gamma}$ secretion were analyzed for the immune monitoring. Therapeutic DCs were cultured from the bone marrow myeloid lineage cells with GM-CSF and IL-4 for 7 days and pulsed with tumor cell lysate for 18 hrs. Results: Compared to the saline treated group, tumor formation was suppressed in 3LL tumor cell lysate pulsed-DC treated group, while 3LL-specific immune stimulation was minimum. WEHI-3-specific immune stimulation occurred in WEHI-3 lysate-pulsed DC treated group, which had no correlation with tumor regression. Conclusion: The data suggest the possible anti-tumor effect of cultured DCs as an adjuvant therapy for minimal residual disease state of lung cancer. The significance of immune modulation in DC therapy including the possible involvement of NK cell as well as antigen-specific cytotoxic T cell activity induction was discussed.
Keywords
Dendritic cells; anti-cancer immunotherapy; lung cancer;
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1 Steinman RM: The dendritic cell system and its role in immunogenicity. Annu Rev Immunol 9;271-296, 1991   DOI   PUBMED   ScienceOn
2 Banchereau J, Steinman RM: Dendritic cells and the control of immunity. Nature 392;245-252, 1998   DOI   ScienceOn
3 Oosterwijk-Wakka J, Tiemessen D, Bleumer I, de Varies I, Jongmans W, Adema G, Debruyne F, de Mulder PH, Oosterwijk E, Mulders PF: Vaccination of patients with metastatic renal cell carcinoma with autologous dendritic cells pulsed with autologous tumor antigen in combination with interleukin-2: A phase I study. J Immunother 25;500-508, 2002   DOI
4 Holtl L, Zelle-Rieser C, Gander H, Papesh C, Ramoner R, Bartsch G, Rogatsch H, Barsoum AL, Coggin JH Jr, Thurnher M: Immunotherapy of metastatic renal cell carcinoma with tumor lysate-pulsed autologous dendritic cells. Clin Cancer Res 8;3369-3376, 2002   PUBMED
5 Marten A, Renoth S, Heinicke T, Albers P, Pauli A, Mey U, Caspari R, Flieger D, Hanfland P, Von Ruecker A, EisHubinger A, Muller S, Schwaner I, Lohmann U, Heylmann G, Sauerbruch T, Schtnidt-Wolf I: Allogeneic dendritic cells fused with tumor cells: preclinical results and outcome of a clinical phase i/ii trial in patients with metastatic renal cell carcinoma. Hum Gene Ther 14;483-494, 2003   DOI   ScienceOn
6 Nestle F, Alijagic S, Gilliet M, Sun Y, Grabbe S, Dummer R, Burg G, Schadendorf D: Vaccination of melanoma patients with peptide- or tumor lysate-pulsed dendritic cells. Nat Med 3;328-332, 1998
7 Banchereau J, Palucka A, Dhodapkar M, Burkeholder S, Taquet N, Rolland A, Taquet S, Coquery S, Wittkowski K, Bhardwaj N, Pineiro L, Steinman R, Fay J: Immune and clinical responses in patients with metastatic melanoma to CD34 (+) progenitor-derived dendritic cell vaccine. Cancer Res 61;6451-6458, 2001   PUBMED
8 Tjoa B, Simmons S, Bowes V, Ragde H, Rogers M, Elgamal A, Kenny G, Cobb O, Ireton R, Troychak M, Salgaller M, Boynton A, Murphy G: Evaluation of phase I/II clinical trials in prostate cancer with dendritic cells and PSMA peptides. Prostate 36;39-44, 1998   DOI   ScienceOn
9 Barrou B, Benit G, Ouldkaci M, Cussenot O, Salcedo M, Agrawal S, Massicard S, Bercovici N, Ericson M, Thiounn N: Vaccination of prostatectomized prostate cancer patients in biochemical relapse with autologous dendritic cells pulsed with recombinant human PSA. Cancer Immunol Imrnunother 53;453-460, 2004   DOI   ScienceOn
10 Hege K, Carbone D: Lung cancer vaccines and gene therapy. Lung Cancer 41;S103-113, 2003   DOI   ScienceOn
11 서광욱, 이봉호,Choti M: 쥐의 전이성 간암 모델에서 자가 종양 백신을 이용한 면역 치료. J Korean Cancer Assoc 31 ;360-366, 1999
12 Hege K, Carbone D: Lung cancer vaccines and gene therapy. Lung Cancer 41;S103-113, 2003   DOI   ScienceOn
13 Katsenelson NS, Shurin GV, Bykovskaia SN, Shogan J, Shurin MR: Human small cell lung carcinoma and carcinoid tumor regulate dendritic cell maturation and function. Mol Pathol 14; 40-45, 2001   DOI   ScienceOn
14 Fields R, Shimizu K, Mule JJ: Murine dendritic cells pulsed with whole tumor Iysates mediate potent antitumor immune responses in vitro and in vivo. Proc Natl Acad Sci USA 95; 9482-9487, 1998
15 Salgia R, Lynch T, Skarin A, Lucca J, Lynch C, Jung K, Dranoff G: Vaccination with irradiated autologous tumor cells engineered to secrete granulocyte-macrophage colonystimulating factor augments antitumor immunity in some patients with metastatic non-small-cell lung carcinoma. J Clin Oncol 21;624-630, 2003   DOI   ScienceOn
16 Yoon HL, Singh K, Ratner S, Reiners JJ: Phorbol ester effects on splenic lymphocyte composition and cytotoxic T cell activities of SSIN mice: a strain deficient in CD8+ T cells. Carcinogenesis 17;2617-2624, 1996   DOI   ScienceOn
17 이진수: 폐암Overview. 대한의사협회지 1;17-11, 2003
18 Hersey P, Menzies S, Halliday G, Nguyen T, Farrelly M, DeSilva C, Lett M: Phase I/ll study of treatment with dendritic cell vaccines in patients with dissetninated melanoma. Cancer Immunol Immunother 53;125-134, 2004   DOI   ScienceOn
19 Kojima T, Yamazaki K, Tamura Y, Ogura S, Tani K, Konishi J, Shinagawa N, Kinoshita I, Hizawa N, Yamaguchi E, Dosaka-Akita H, Nishimura M: Granulocyte-macrophage colonystimulating fetor gene-transduced tumor cells combined with tumor-derived gp96 inhibit tumor growth in mice. Hum Gene The 14;715-728, 2003
20 Kemp R, Ronchese F: Tumor-specific Tel, but not Tc2, cells deliver protective antitumor immunity. J Immunol 167; 64976502, 2001
21 Lee H: Dendritic cell based cancer immunotherapy: The 2nd International Symposium on Stem Cells and Cell Therapy May 1, 2004
22 Sharma S, Miller P, Stolina M, Zhu L, Huang M, Paul R, Dubinett S: Multicomponent gene therapy vaccines for lung cancer : effective eradication of established murine tumors in vivo with interleukin-7/herpes simplex thytnidine kinasetransduced autologous tumor and ex vivo activated dendritic cells. Gene Ther 4;1361-1370, 1997   DOI   ScienceOn
23 Paglia P, Chiodoni C, Rodolfo M, Colombo MP: Murine dendritic cells loaded in vitro with soluble protein prime cytotoxic T lymphocytes against tumor antigen in vivo. J Exp Med 183;317-322, 1996   DOI   ScienceOn
24 한국중앙암등록사업 연례 보고서- 보건복지부 한국중앙 암 등록본부, 2002
25 Palucka K, Banchereau J: Linking innate and adaptive immunity. Nat Med 5;868-870, 1999   DOI   ScienceOn
26 Murphy G, Tjoa B, Simmons S, Jarisch J, Bowes V, Ragde H, Rogers M, Elgamal A, Kenny G, Cobb O, Ireton R, Troychak M, Salgaller M, Boynton A: Infusion of dendritic cells pulsed with HLA-A2-specific prostate-specific mernbrane antigen pep tides: a phase II prostate cancer vaccine trial involving patients with hormone-refractory metastatic disease. Prostate 38;73-78, 1999   DOI   ScienceOn
27 Fong L, Hou Y, Rivas A: Altered peptide ligand vaccination with Flt3 ligand expanded dendritic cells for tumor immunotherapy. Proc Natl Acad Sci USA 98;8809-8814, 2001
28 Dematos P, Abdel-Wahab Z, Vervaert C, Hester D, Seigler H: Pulsing of dendritic cells with cell Iysates from either B 16 melanoma or MCA -106 fibrosarcoma yields equally dffective vaccines against B16 tumors in mice. J Surg Oncol 68;79-91, 1998   DOI   ScienceOn
29 권희충, 정재민, 김정현, 함용호, 서지숙, 이기호, 김장민, 이한수, 이춘택: Lewis 폐암 마우스 모델 에서 Rerivort Vector나 Adenoviral Vector로 이입된 Herpes Simplex Virus Thymidine Kinase 유전자 치료. 결핵 및 호흡기 질환 49; 298-309, 2000
30 Lee H, In S, Kim M, Baek S, and Lee S: Antitumor immunity modulated by IL-10 producing dendritic cells pulsed with Lewis lung carcinoma cell lysates: Proceedings of 95th Annual meeting of American Association for Cancer Research 45, 2004
31 Fernandez N, Lozier A, Flament C, Ricciardi-Castagnoli P, Bellet D, Suter M, Perricandet M, Tursz T, Maraskovsky E, Zitvogel L: Dendritic cells directly trigger NK cell functions: Cross-talk relevant in innate anti-tumor immune responses in vivo. Nature Medicine 4;405-410, 1999
32 Park K, Kim G, Jang S, Kim C, Kwon S, Yoo C, Kim Y, Kwon H, Kim C, Han S, Shim Y, Lee C: Genetherapy with GM-CSF, interleukin-4 and herpes simplex virus thymidine kinase shows strong antitumor effect on lung cancer. Anticancer Res 23;1559-1564, 2003   PUBMED
33 Zeid N, Muller H: S100 positive dendritic cells in human lung tumors associated with cell differentiation and enhanced survival. Pathology 25;338-343, 1993   DOI   ScienceOn
34 Krause S, Neumann C, Soruri A, Mayer S, Peters J, Andreesen R: The treatment of patients with disseminated malignant melanoma by vaccination with autologous cell hybrids of tumor cells and dendritic cells. J Immunother 25;421-428, 2002   DOI
35 Zhou Y, McEarchern J, Howard E, Prestano G, Salgaller M, Bosch M: Dendritic cells efficiently acquire and present antigen derived from lung cancer cells and induce antigenspecific T-cell responses. Cancer Immunol Immunother 52, 413-422, 2003   DOI   ScienceOn
36 Smithers M, O'Connell K, MacFadyen S, Chambers M, Greenwood K, Boyce A, Abdul-Jabbar I, Barker K, Grimmett K, Walpole E, Thomas R: Clinical response after intradermal immature dendritic cell vaccination in metastatic melanoma is associated with immune response to particulate antigen. Cancer Immunol Immunother 52;41-52, 2003
37 Tjoa B, Erickson S, Bowes V, Ragde H, Kenny G, Cobb O, Ireton R, Troychak M, Boynton A, Murphy G: Follow-up evaluation of prostate cancer patients infused with autologous dendritic cells pulsed with PSMA peptides. Prostate 32;272-278, 1997   DOI   ScienceOn
38 Hirschowitz E, Foody T, Kryscio R, Dickson L, Sturgill J, Yannelli J: Autologous dendritic cell vaccine for non-smallcell lung cancer. J Clin Oncol 22;2808-2815, 2004   DOI   ScienceOn
39 Specht J, Wang G, Do M, Lam J, Royal R, Reeves M, Rosenberg S, Hwu P: Dendritic cells retrovirally transduced with a model antigen gene are therapeutically effective against established pulmonary metastasis. J Exp Med 186;1213-1221, 1997   DOI   ScienceOn
40 Palucka K, Banchereau J: Dendritic cells: a link between innate and adaptive immunity. J Clin Immunol 19;12-25, 1999   DOI   ScienceOn
41 Gitlitz B, Belldegrun A, Zisman A, Chao D, Pantuck A, Hinkel A, Mulders P, Moldawer N, Tso C, Figlin R: A pilot trial of tumor lysate-loaded dendritic cells for the treatment of metastatic renal cell carcinoma. J Immunother 26;412419, 2003
42 Young M, Wright M, Vellody K, Lathers D: Skewed differentiation of bone marrow CD34+ cells of tumor bearers from dendritic toward monocytic cells, and the redirection of differentiation toward dendritic cells by 1 alpha, 25-dihydroxyvitamin D3. Int J Immunopharmaol 21;675-688, 1999   DOI   ScienceOn
43 Haenssle H, Krause S, Emmert S, Zutt M, Kretschmer L, Schtnidberger H, Andreesen R, Soruri A: Hybrid cell vaccination in metastatic melanoma: clinical and immunologic results of phase I/II study. J Immunother 27;147-155, 2004   DOI
44 Inaba K, lnaba M, Romani N, Aya H, Dequchi M, Ikehara S, Steinman RM: Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J Exp Med 176;1693-1702, 1992   DOI   ScienceOn
45 Azuma T, Horie S, Tomita K, Takahashi T, Tanaka Y, Kashiwase K, Nieda M, Takeuchi T, Ohta N, Shibata Y, Hirai H, Kitamura T: Dendritic cell immunotherapy for patients with metastatic renal cell carcinoma: University of Tokyo experience. Int J Urol 9;340-6, 2002   DOI   ScienceOn
46 Sharma S, Yang S, Batra R, Dubinett S: Intratumoral therapy with cytokine gene-modified dendritic cells in murine lung cancer models. Methods Mol Med 75;711-22, 2003   PUBMED
47 Neuner A, Schindel M, Wildenberg U, Muley T, Lahm H, Fischer J: Cytokine secretion: clinical relevance of immunosuppression in non-small cell lung cancer. Lung Cancer 34; S79-S82, 2001   DOI   ScienceOn
48 Inoshima N, Nakanishi Y, Minami T, Izutni M, Takayama K, Yoshino I, Hara N: The influence of dendritic cell infiltration and vascular endithelial growth factor expression on the prognosis of non-small-cell lung cancer. Clin Cancer Res 8;3480-3486, 2002   PUBMED
49 이현아, 최광민, 백소영, 이홍기, 정철원: 수지상 세포를 이용한 항암 면역 치료. 생쥐 신장암 모델을 이용한 연구. Immune Network 4;44-52, 2004   DOI