• 분석과학
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• 제31권1호
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• pp.7-13
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• 2018

이 연구에서는 구석기 고토양층 석영의 시간분해 광자극 냉광 신호를 측정한 후 측정된 냉광신호로부터 시료의 냉광수명을 산출하였다. 냉광수명의 방사선 조사 후 열전처리 의존성 및 조사선량 의존성, 광자극 펄스 의존성을 모두 고려한 결과, 고토양층 석영의 시간분해 광자극 냉광은 방사선량 100 Gy 조사 후 별도의 열전처리 없이 펄스 주기 $250{\mu}s$, 펄스 폭 $10{\mu}s$, 반복수 100,000번인 광 자극 펄스를 가하여 측정할 때 정확한 냉광수명이 산출됨을 알 수 있었다. 냉광수명의 측정온도 의존성으로부터 산출된 열소광 활성화 에너지 ${\Delta}E$, thermal assistance 활성화 에너지 $E_a$는 각각 $0.60{\pm}0.14eV$, $0.053{\pm}0.029eV$ 이었으며, 이 결과는 기존에 보고된 연구 성과와 매우 잘 일치하였다. 결론적으로 이 연구에서 산출된 고토양층 석영의 운동학 변수 값은 매우 신뢰할 수 있다고 판단된다.

• 한국가축번식학회지
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• 제18권2호
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• pp.113-119
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• 1994

To improve nuclear transplantation(NT) efficiency and to produce a large scale genetically identical cloned calves, examined the in vitro development capacity after co-culture of bovine oviductal epithelial cells (BOEC) and granulosa cells in TCM-199 supplemented with 10% fetal calf serum (FCS) with early bovine embryos derived from in vitro matured fertilized(IVM-IVF) oocyte. In addition, the age dependence of IVM oocyte on electro-stimulation and the effective electric voltage on in ivtro development of bovine NT embryos were examined. The results obtained were summerized as follows; 1. The cleavage rates of IVM-IVF bovine embryos in co-culture with bovine oviductal epithelial cells and granulosa cells were not significantly different(P<0.05), but the developmental rate into morula and blastocyst stage were different showing 38.3 and 20.2%, respectively. 2. The activation (82.5%) and development in vitro(8.6%) into later embryo stages of the aging oocytes of 32 hours post-maturation (hpm) were significantly higher than those of 24 hpm at direct current (DC) voltage of 1.5kV/cm, 60$\mu$sec pulse duration and 1 pulse time. 3. The fusion rates of NT eggs of 32 hpm following to different DC voltages from range 0.75 to 1.5kV/cm were not differ, but the developmental rate into morula and blastocyst stages at DC voltages of 0.75 and 1.0kV/cm were higher(11.4 and 12.6%, respectively) than those of 1.5kV/cm(0%). From these results, it can be suggested the optimal culture system for in vitro culture of IVM-IVF bovine embryos is a co-culture system with BOEC in TCM-199 supplemented 10% FCS. The effective time and the DC voltage for activation, electrofusion and in vitro development of NT embryos derived from IVM-IVF bovine embryo are 32hpm and 0.75~1.0kV/cm. But to improve NT efficiency, the advanced research (cell cycle synchronization, micromanipulation, culture system, etc.) is needed.

• Reproductive and Developmental Biology
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• 제30권1호
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• pp.1-5
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• 2006

핵이식 방법을 이용하여 성공적인 복제를 이루기 위해서 인위적인 활성화 처리는 필수적인 요소이다. 본 연구는 전기자극에 의해 활성화된 난자를 chemical agent를 이용하여 추가적인 활성화 처리를 하였을 때 돼지 단위발생란의 발달에 미치는 영향을 알아보고자 수행되었다. 체외에서 $40{\sim}44$시간 동안 배양된 난자를 전기자극(E)으로 활성화 처리한 후 Thimerasol + Dithiothreitol(Thi+DTT), 6-Dimethylaminopurine(6-DMAP) 및 Cycloheximide(CH)를 사용하여 추가 활성화 처리를 하였다. 활성화 방법(E, E+Thi+DTT, E+6-DMAP 및 E+CH)에 따른 단위발생란의 배반포까지의 발달율을 조사한 결과, chemical agent에 의해 추가 활성화된 단위발생란이 전기자극만으로 처리된 구의 단위발생란보다 유의적으로 높은 발달율을 보였다($21.5{\sim}28.1%$ vs. 18.0%, P<0.05). 특히, E+Thi+DTT를 이용하였을 때 발달율이 유의적으로 높게 나타났다(28.1%, P<0.05). 활성화 처리별 전핵 형성율을 조사한 결과, chemical agent에 의해 추가 활성화 처리된 구에서 하나의 극체(1PN) 형성률은 처리별로 차이를 보이지 않았으나$(59.9{\sim}64.7%)$, 2PN 형성율은 추가 활성화 처리구에서 전기자극만을 사용하였을 때보다 유의적으로 높게 나타났다($7.2{\sim}9.7%$ vs. 4.3%, P<0.05). 이상의 결과를 살펴볼 때, 전기자극 후 chemical agent를 이용한 추가 활성화는 단위발생란의 배반포까지의 발달능력을 증가시키는 것으로 생각된다.

• 한국자기학회지
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• 제21권2호
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• pp.48-51
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• 2011

본 논문에서는 짧은 전류 펄스를 이용한 미소자기 소자에서의 전류 유도 자화 반전에 대한 매크로 스핀 시뮬레이션 연구를 수행하였다. 자화 반전 전류 분포에 있어서 에너지 장벽이 미치는 효과에 특별히 주목하였다. 자화 반전 전류의 크기 및 분포는 전류 펄스 폭의 감소에 따라 증가했다. 여기서 긴 전류 펄스 폭 영역에서는 에너지 장벽과 자화 반전 전류 분포 사이의 관계가 아레니우스-닐 법칙에 의해 서술된다. 하지만 짧은 전류 펄스 폭의 영역에서는 이 관계가 풀리지 않은 채로 남아있다. 이는 짧은 전류 펄스로 인한 자화 반전이 열적 활성화에 의해서가 아닌 세차 운동에 의해 좌우되기 때문이며, 이를 해결하는 데에 있어서 어려움이 발생한다. 그러므로 포커-플랑크 방정식을 풀어서 짧은 전류 펄스 영역에서의 자화 반전에 대한 정확한 공식을 얻어내는 것이 필요하며 이를 통해 짧은 전류 펄스 영역에서의 자화 반전 양상을 이해 할 수 있을 것으로 본다.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 1997년도 제8회 학술강연회논문집
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• pp.259-265
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• 1997

An analytical study has been peformed to investigate the unsteady ignition characteristics of pulse combustion. In many combustion applications, strain rate of the flow can significantly affect the combustion features; ignition, extinction, and reignition. In the pulse combustion, two jets (hot combustion gases and fresh mixtures) coming from the opposite side of the combustor will collide in the combustor forming a stagnation region where the chemical reaction is suppressed by the strain rate until this becomes below the critical value. In this research, the method of large activation energy asymptotic is adopted with one step irreversible kinetics to examine the ignition response to the periodic variation of the strain rate of flow. The results show the variation of the maximum value of strain rate can determine whether the ignition or extinction occur.

• 한국추진공학회지
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• 제1권1호
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• pp.64-72
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• 1997

맥동연소의 비정상 점화현상을 연구하기 위하여 이론적인 해석을 수행하였다. 맥동연소에서는 연소기의 양쪽에서 유입되는 고온의 연소가스와 미연 혼합가스가 연소실 내부에서 충돌하여 정체면을 형성하며 유동변형율이 임계 값 이하가 될 때까지 점화가 억제된다. 본 연구에서는 유동의 유동변형율의 변화에 대한 점화현상의 반응을 연구하기 위하여 활성화 에너지 점근법과 비가역 1단계 화학반응을 이용하였다. 또한 유동에 의한 유동변형율은 두 가지 요인에 의하여 발생하는 것으로 모델링 하였는데, 비정상 유동에 의한 평균 유동변형율과 난류에 의하여 유도되는 유동변형율이 그것이다. 해석 결과에 의하면, 맥동연소에서는 잘 정의된 점화지연이 존재하며, 점화 또는 소염의 발생 여부는 Damkohler 수에 의하여 거의 결정된다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 하계종합학술대회 논문집(2)
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• pp.367-370
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• 2004

We fabricated PLT(5) thin film on $Pt/TiO_x/SiO_2/Si$ substrate by using sol-gel method and investigated leakage current, switching and retention properties. The leakage current density of PLT(5) thin film was $3.56{\times}10^{-7}A/cm^2$ at 4V. In the examination of switching properties, pulse voltage and load resistance were $2V{\~}5V$ and $50{\Omega}{\~}3.3k{\Omega}$, respectively. Switching time had a tendency to decrease from 520ns to 140ns with the increase of pulse voltage, and also the time was increased from 140ns to $13.7{\mu}s$ with the increase of load resistance. The activation energy obtained from the relation of applied pulse voltage and switching time was about 143kV/cm. The error of switched charge density between hysteresis loop and experiment of polarization switching was about $10\%$. Also, polarization in retention was decreased as much as about $8\%$ after $10^5$s.

• 한국대기환경학회지
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• 제15권4호
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• pp.475-483
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• 1999

A lab-scale test was carried out to study the reduction of electrical energy consumption in the pulsed corona discharge process for nitrogen oxides removal. The experiment was mainly focused on 1) the activation of pollution removal reactions by chemical additives and 2) the optimization of electrical circuit for the efficient energy transfer from the power supply to the corona reactor. Hydrocarbon chemical additives used in the experiment are thought to be responsible for the enhancement of the NO conversion through the chain reactions of free radicals such as, R, RCO, and RO. Electrical energy consumption per converted NO molecule has a minimum value of 17 eV when pentanol is injected. When ethylene and propylene are injected, 30 eV and 22 eV of electrical energy consumption is required for the conversion of NO molecule respectively. The ratio of the pulse forming capacitance$(C_e)$ to the reactor capacitance$(C_R)$ plays an important role in the energy transfer efficiency to the reactor. Maximum energy transfer efficiency of approximately 72% could be obtained by using the pulse forming capacitance which is 3.4 times larger than the reactor capacitance, and also the maximum NO conversion efficiency was observed with the same condition.

• 한국가축번식학회지
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• 제19권3호
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• pp.171-179
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• 1995

This study was carried out to investigate the fertilizing ability of round spematids isolated from seminiferous tubules. A round spermatid was introduced into the perivitelline space of a mature oocyte using Leitz micromanipulators and then subjected to electrofusion. Electrofusion was induced by applying a single DC pulse of 90V with a duration of 60$\mu$sec using Model 611 Square Wave Stimulator(Phipps and Bird, U.S.A) in 0.3 M sucrose fusion medium containing 0.05mM CaCl2 and 0.1mM MgSO4, Oocyte pre-activation was conducted by exposure to a single DC(80V, 80$\mu$sec) pulse in electrofusion medium at 1 hour before electrofusion. The incidence of fusion with pre-activated oocytes(23.8%, 57/239) was higher than that with nonactivated oocytes(6.7%, 3/45). The most of electro-stimulated mouse oocytes cleaved regardless of the success or failure of fusion. Karyotyping of embryos that developed into blastocysts after exposure to the fusion pulse were performe. We found that blastocysts from the fused oocytes were diploid whereas blastocysts from the unfused oocytes were haploid. About 11.7 and 11.5% of fused and unfused oocytes were developmental potentials of fused and unfused oocytes. Therefore, these results suggest that the mouse mture oocyte can be fertilized by fusion with a round spermtid and subsequently developed normally.

• The Korean Journal of Physiology
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• 제24권2호
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• pp.377-388
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• 1990

Ca movements during the late plateau phase in rabbit atrium implicate Na-Ca exchange. In single atrial cells isolated from the rabbit the properties of the inward current of Na-Ca exchange were investigated using the whole cell voltage clamp technique. The inward currents were recorded during repolarization following brief 2 ms depolarizing pulse to +40 mV from a holding potential of -70 mV. Followings are the results obtained: 1) When stimulated every 30 sec, the inward currents were activated and reached peak values $6{\sim}12\;ms$ after the beginning of depolarizing pulse. The mean current amplitude was 342 pA/cell. 2) The current decayed spontaneously from the peak activation and the timecourse of the relaxation showed two different phases: fast and slow phase. 3) The recovery of the inward current was tested by paired pulse of various interval. The peak current recovered exponentialy with a time course similar to that of Ca current recovery. 4) Relaxation timecourse was also affected by pulse interval and time constant was reduced almost linearly according to the decrease of pulse interval between 30 sec and 1 sec. 5) The peak inward current was increased by long prepulse stimulation, Bay K, isoprenaline or c-AMP. 6) The relaxation time constant of the inward current was prolonged by Bay K or c-AMP, and shortened by isoprenaline. From the above results, it could be concluded that increase of the calcium current potentiates and prolongs intracellular calcium transients, while shortening of the timecourse by isoprenaline or short interval stimulations might be due to the facilitation of Ca uptake by SR.