• 동국한의학연구소논문집
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• 제5권
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• pp.197-207
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• 1996

As a mood-altering drug, long-term alcohol consumption have significant harmful effects on the human body and people's mental functioning. This study observed that the suppression of cell mediated immunity induced in spleen of ICR mouse by long-term alcohol administration. After 8% alcohol voluntary administered for 120 days, the splenic tissue irnmunohistochemically stained by following ABC method that used monoclonal antibody including L3T4(CD4), Ly-2(CD8), IL-2 receptor(CD25R) and NK-1.1(CD56) after embedding with paraffin. The results were as follows. 1. The size of marginal zone in splenic white pulp was diminished and the number of macrophage in marginal zone was decreased in test group than control group. 2. After alcohol administration, the number of Helper T lymphocyte, cytotoxic T lymphocyte, and IL-2 receptor were decreased in periarterial lymphatic sheaths of white pulp and penicilla artery of red pulp and the degree of CD4, CD8, and CD25R positive reaction were soften. 3. In test group, the number of NK cell were decreased. These results indicated that the secretion of lymphokine as IL-2 was inhibited by long-term alcohol administration and subsequently prevent to activate and proliferate splenic T lymphocytes and NK cells as cell mediated immunity component.

• 펄프종이기술
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• 제46권2호
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• pp.35-45
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• 2014

Confocal Reflection Microscopy (CRM) was applied to investigate external fibrillation of different types of fibers such as Kajaani reference fiber, Whatman filter fiber, thermomechanical pulp (TMP), and recycled TMP fiber. It was confirmed that the CRM images are created from surface structures of the fiber cell wall. Confocal Laser Scanning Microscopy (CLSM) captured overall shape of the fiber, but minute details of the surface of the fiber were missed. CRM captured the minute details of the fiber surface. From the CRM and CLSM images, it was observed that the CRM images mainly appeared on the fiber surfaces. External fibrillation of the fiber occurs at the fiber surface, not inside the cell wall. Thus, it was concluded that investigation on the external fibrillation of the fiber was possible by utilizing CRM images. A direct qualtitative and quantitative method for analysis of external fibrillation of fiber was demonstrated by utilizing surface area to volume ratio, volume fraction, and roughness calculated from 3-dimensional images reconstructed from stacks of CRM images from the different fibers.

• KSBB Journal
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• 제8권4호
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• pp.351-357
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• 1993

펄프 폐수처리를 위한 효과적인 생물학적 처리법 으로서 세 가지 고정화 방법과 자유균체를 이용한 방법을 사용하여 실험하였다. 균체로서는 Phaηe­r rochaete chrysosporium을 이용하였다. 고정화방법 중 Ca-alginate gel을 담체로 샤용한 방법이 가장 우수하였다. 이 방법은 안정성과 처리 효율변에서 좋은 결과를 보였으며, 약 400시간 동안 균체의 커다란 상태변화없이 반응시킬 수 있었다. 다른 고정화 방법들과 자유균체를 이용한 방법들 의 가장 큰 문제점은 생물 반응기 안에서의 공기 c channelling 현상에 의한 산소전달 저항과 균체끼리 의 clogging으로 인한 반응기 내부의 제어가 힘들게 된 것이었다.

• BMB Reports
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• 제55권7호
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• pp.336-341
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• 2022

Narrowing of arteries supplying blood to the limbs provokes critical hindlimb ischemia (CLI). Although CLI results in irreversible sequelae, such as amputation, few therapeutic options induce the formation of new functional blood vessels. Based on the proangiogenic potentials of stem cells, in this study, it was examined whether a combination of dental pulp stem cells (DPSCs) and human umbilical vein endothelial cells (HUVECs) could result in enhanced therapeutic effects of stem cells for CLI compared with those of DPSCs or HUVECs alone. The DPSCs+ HUVECs combination therapy resulted in significantly higher blood flow and lower ischemia damage than DPSCs or HUVECs alone. The improved therapeutic effects in the DPSCs+ HUVECs group were accompanied by a significantly higher number of microvessels in the ischemic tissue than in the other groups. In vitro proliferation and tube formation assay showed that VEGF in the conditioned media of DPSCs induced proliferation and vessel-like tube formation of HUVECs. Altogether, our results demonstrated that the combination of DPSCs and HUVECs had significantly better therapeutic effects on CLI via VEGF-mediated crosstalk. This combinational strategy could be used to develop novel clinical protocols for CLI proangiogenic regenerative treatments.

• Restorative Dentistry and Endodontics
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• 제43권4호
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• pp.36.1-36.12
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• 2018

Objectives: Direct pulp capping is a treatment for mechanically exposed pulp in which a biocompatible capping material is used to preserve pulpal vitality. Biocompatibility tests in animal studies have used a variety of experimental protocols, particularly with regard to the exposure site. In this study, pulp exposure on the occlusal and mesial surfaces of molar teeth was investigated in a rat model. Materials and Methods: A total of 58 maxillary first molars of Wistar rats were used. Forty molars were mechanically exposed and randomly assigned according to 3 factors: 1) the exposure site (occlusal or mesial), 2) the pulp-capping material (ProRoot White MTA or Bio-MA), and 3) 2 follow-up periods (1 day or 7 days) (n = 5 each). The pulp of 6 intact molars served as negative controls. The pulp of 12 molars was exposed without a capping material (n = 3 per exposure site for each period) and served as positive controls. Inflammatory cell infiltration and reparative dentin formation were histologically evaluated at 1 and 7 days using grading scores. Results: At 1 day, localized mild inflammation was detected in most teeth in all experimental groups. At 7 days, continuous/discontinuous calcified bridges were formed at exposure sites with no or few inflammatory cells. No significant differences in pulpal response according to the exposure site or calcium-silicate cement were observed. Conclusions: The location of the exposure site had no effect on rat pulpal healing. However, mesial exposures could be performed easily, with more consistent results. The pulpal responses were not significantly different between the 2 capping materials.

• 펄프종이기술
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• 제41권2호
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• pp.47-54
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• 2009

Anatomical characteristics of kenaf were investigated in transverse, radial and tangential direction by optical and scanning electron micrograph. Kenaf was made up of bast fibers, wood fibers, vessels and parenchyma cells. Bast fibers were long slender cells with different types of pits. The shape of wood fibers were in various ways and pointed at the ends. The pits were observed on the surface of bast fibers. Kenafs were diffuse and radial porous. and composed of solitary pores and two or three radial pore multiples. Various types of vessels were observed. The pits showed alternate pitting and larger diameter than other cells. Parenchyma cells were rectangular or square with different shapes of pith parenchyma cells compared to conventional types of parenchyma cells in wood. The number of pith on the surfaces were small.

• 펄프종이기술
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• 제42권2호
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• pp.27-34
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• 2010

To obtain the basic information for the use of bamboo, the anatomical characteristics of moso bamboo shoots, and 20-day-old, 60-day-old, one-year-old, and two-year-old moso bamboo were observed by using polarizing microscope. The cross section showed that the bamboo shoots consists of the early stage of cell formation, atactostele, parenchyma, and bundle sheath was formed in 20-day-old moso bamboo, and all cells completely formed in 60-day-old moso bamboo. The tissues in one-year-old moso bamboo appeared to be completely matured. On the tangential surface, no atactostele was observed in the bamboo shoot, but metaxylem, parenchyma, and bundle sheath were found. atactostele and parenchyma appeared to be formed between 20 and 60 days. All tissues and cells were totally formed and the cell wall of parenchyma were completely matured after one year. The metaxylem in bamboo shoots was observed to have pits, which means that it could be the tissues first formed of all bamboo cells.

• 펄프종이기술
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• 제31권2호
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• pp.70-76
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• 1999

This study was to measure the potential of nonwoody fibrous material, kenaf. Whole stalk of kenaf, Hibiscus cannabinus was separated by two parts of bast and core portion, and cooked separately by alkaline method. Morphological characteristic was evaluated using confocal laser scanning microscope (CLSM) and fiber quality analyzer(FQA). The strength properties of handsheets, made by different mixing ration between kenaf base and core fibers, were measured. Cross-sectional area of bast fibers was smaller than that of core fibers, but the bast fibers had a thick cell wall and narrow lumen area. Bast fibers were longer in length than core fibers. Core fibers had thin cell walls, broad lumen areas, and short lengths, and they had collapsed shape even in water. These characteristics of core fibers affected strength properties of handsheet positively. When the amount of core fibers increased, the strength properties of handsheet were increased. When the amount of bast fibers increased, the handsheet had rougher surface and higher air permeability.

• 펄프종이기술
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• 제43권1호
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• pp.29-35
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• 2011

Since the role of lignin in the wood cell wall is to keep integrity and structure rigidity of lignocellulosic substrate, lignin of the cell wall has to be destroyed before enzymatic hydrolysis of wood polysaccharides. The aspen wood meals were delignified with ozone in acidic condition. The chemical characteristics of wood meal were investigated. The 60% of lignin and almost zero % of polysaccharides in aspen wood meal was degraded with 10min. ozone treatment. The phenolic hydroxyl groups of lignin in ozonated wood meal were increased with ozonation time. The sugar composition of ozonated wood meal showed that the hemicellulose was more susceptible to ozonation than cellulose. The yield of aldehyde was increased in some degree with 10min. ozone treatment and decreased with longer ozone treatment.

• Restorative Dentistry and Endodontics
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• 제21권1호
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• pp.35-53
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• 1996

The development and repair requires the formation of new tissues comprised of various extracellular matrix components. The present study investigated the formation and distribution of the major ECM components such as type I collagen, type III collagen, fibronection, bone sialoprotein, and osteonection during development and repair. For developing observation. Sprague-Dawley rats weighing $27{\pm}1gm$ were sacrificed. For repair observation, Sprague-Dawley rats weighing $110{\pm}5gm$ were used. The pulp perforation were prepared on mesial surface of the maxillary first molar by using 1/2round bur. At 5 days after perforation, rats were sacrificed by perfusion with 3 % paroformaldehyde. The maxillary first molar region were cut, demineralized, dehydrated and embedded in paraffin. Immunostaining the ECM components was achieved by the avidin-biotin complex method. The results as follows : 1. Bright immunoreaction for fibronectin was present in the basement membrane at the inner epithelial-mesenchymal interface, especially concentrated in the blood vessel walls, cell membrane of odontoblasts, and initial predentin. 2. Type I and III collagen was observed in the newly formed pulp tissue, predentin, and its intensity increased as more of these components during repair. 3. Strong immunostaining for bone sialoprotein and osteonectin was found in dentin while no or weaker staining was observed loose connective tissue of the pulp. 4. These results suggest that develpment and repair is achieved through a series of cell differentiation and attachment by the specific ECM components.