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Effects of the exposure site on histological pulpal responses after direct capping with 2 calcium-silicate based cements in a rat model

  • Trongkij, Panruethai (Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University) ;
  • Sutimuntanakul, Supachai (Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University) ;
  • Lapthanasupkul, Puangwan (Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University) ;
  • Chaimanakarn, Chitpol (Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University) ;
  • Wong, Rebecca (Restorative Section, Melbourne Dental School, University of Melbourne) ;
  • Banomyong, Danuchit (Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University)
  • Received : 2018.03.21
  • Accepted : 2018.07.02
  • Published : 2018.11.30

Abstract

Objectives: Direct pulp capping is a treatment for mechanically exposed pulp in which a biocompatible capping material is used to preserve pulpal vitality. Biocompatibility tests in animal studies have used a variety of experimental protocols, particularly with regard to the exposure site. In this study, pulp exposure on the occlusal and mesial surfaces of molar teeth was investigated in a rat model. Materials and Methods: A total of 58 maxillary first molars of Wistar rats were used. Forty molars were mechanically exposed and randomly assigned according to 3 factors: 1) the exposure site (occlusal or mesial), 2) the pulp-capping material (ProRoot White MTA or Bio-MA), and 3) 2 follow-up periods (1 day or 7 days) (n = 5 each). The pulp of 6 intact molars served as negative controls. The pulp of 12 molars was exposed without a capping material (n = 3 per exposure site for each period) and served as positive controls. Inflammatory cell infiltration and reparative dentin formation were histologically evaluated at 1 and 7 days using grading scores. Results: At 1 day, localized mild inflammation was detected in most teeth in all experimental groups. At 7 days, continuous/discontinuous calcified bridges were formed at exposure sites with no or few inflammatory cells. No significant differences in pulpal response according to the exposure site or calcium-silicate cement were observed. Conclusions: The location of the exposure site had no effect on rat pulpal healing. However, mesial exposures could be performed easily, with more consistent results. The pulpal responses were not significantly different between the 2 capping materials.

Keywords

References

  1. Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol 1965;20:340-349. https://doi.org/10.1016/0030-4220(65)90166-0
  2. ISO. ISO 7405: 2008 Dentistry - Evaluation of biocompatibility of medical devices used in dentistry. Part 6: test procedures specific to dental materials. 2nd ed. New York (NY): International Organization for Standardization; 2008. p19-27.
  3. Dammaschke T. Rat molar teeth as a study model for direct pulp capping research in dentistry. Lab Anim 2010;44:1-6. https://doi.org/10.1258/la.2009.008120
  4. Decup F, Six N, Palmier B, Buch D, Lasfargues JJ, Salih E, Goldberg M. Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat's molar. Clin Oral Investig 2000;4:110-119. https://doi.org/10.1007/s007840050126
  5. Six N, Lasfargues JJ, Goldberg M. Differential repair responses in the coronal and radicular areas of the exposed rat molar pulp induced by recombinant human bone morphogenetic protein 7 (osteogenic protein 1). Arch Oral Biol 2002;47:177-187. https://doi.org/10.1016/S0003-9969(01)00100-5
  6. Louwakul P, Lertchirakarn V. Response of inflamed pulps of rat molars after capping with pulp-capping material containing fluocinolone acetonide. J Endod 2015;41:508-512. https://doi.org/10.1016/j.joen.2014.12.004
  7. Dammaschke T, Stratmann U, Fischer RJ, Sagheri D, Schafer E. A histologic investigation of direct pulp capping in rodents with dentin adhesives and calcium hydroxide. Quintessence Int 2010;41:e62-e71.
  8. Hayashi K, Handa K, Koike T, Saito T. The possibility of genistein as a new direct pulp capping agent. Dent Mater J 2013;32:976-985. https://doi.org/10.4012/dmj.2013-091
  9. Liu S, Wang S, Dong Y. Evaluation of a bioceramic as a pulp capping agent in vitro and in vivo. J Endod 2015;41:652-657. https://doi.org/10.1016/j.joen.2014.12.009
  10. Long Y, Liu S, Zhu L, Liang Q, Chen X, Dong Y. Evaluation of pulp response to novel bioactive glass pulp capping materials. J Endod 2017;43:1647-1650. https://doi.org/10.1016/j.joen.2017.03.011
  11. Kawashima S, Shinkai K, Suzuki M. Effect of an experimental adhesive resin containing multi-ion releasing fillers on direct pulp-capping. Dent Mater J 2016;35:479-489. https://doi.org/10.4012/dmj.2015-381
  12. Suzuki M, Taira Y, Kato C, Shinkai K, Katoh Y. Histological evaluation of direct pulp capping of rat pulp with experimentally developed low-viscosity adhesives containing reparative dentin-promoting agents. J Dent 2016;44:27-36. https://doi.org/10.1016/j.jdent.2015.11.005
  13. Berman DS, Massler M. Experimental pulpotomies in rat molars. J Dent Res 1958;37:229-242. https://doi.org/10.1177/00220345580370020701
  14. Paranjpe A, Zhang H, Johnson JD. Effects of mineral trioxide aggregate on human dental pulp cells after pulp-capping procedures. J Endod 2010;36:1042-1047. https://doi.org/10.1016/j.joen.2010.02.013
  15. Takita T, Hayashi M, Takeichi O, Ogiso B, Suzuki N, Otsuka K, Ito K. Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells. Int Endod J 2006;39:415-422. https://doi.org/10.1111/j.1365-2591.2006.01097.x
  16. Ford TR, Torabinejad M, Abedi HR, Bakland LK, Kariyawasam SP. Using mineral trioxide aggregate as a pulp-capping material. J Am Dent Assoc 1996;127:1491-1494. https://doi.org/10.14219/jada.archive.1996.0058
  17. Faraco IM Jr, Holland R. Response of the pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement. Dent Traumatol 2001;17:163-166. https://doi.org/10.1034/j.1600-9657.2001.170405.x
  18. Nair PN, Duncan HF, Pitt Ford TR, Luder HU. Histological, ultrastructural and quantitative investigations on the response of healthy human pulps to experimental capping with mineral trioxide aggregate: a randomized controlled trial. Int Endod J 2008;41:128-150.
  19. Yun YR, Yang IS, Hwang YC, Hwang IN, Choi HR, Yoon SJ, Kim SH, Oh WM. Pulp response of mineral trioxide aggregate, calcium sulfate or calcium hydroxide. J Korean Acad Conserv Dent 2007;32:95-101. https://doi.org/10.5395/JKACD.2007.32.2.095
  20. Settawacharawanich S, Sutimuntanakul S, Phuvaravan S, Plang-ngern S. The chemical compositions and physicochemical properties of a Thai white Portland cement [Master's thesis]. Bangkok: Mahidol University; 2006.
  21. Pisalchaiyong N, Sutimuntanakul S, Korsuwannawong S, Vajrabhaya L. Evaluating cytotoxicity of Thai white Portland cement in cell culture using MTT assay. Mahidol Dent J 2010;30:17-26.
  22. Chaimanakarn C, Sutimuntanakul S, Jantarat J. Subcutaneous tissue response to calcium silicate-based cement [Master's thesis]. Bangkok: Mahidol University; 2014.
  23. Warotamawichaya S, Sutimuntanakul S. Effect of calcium chloride on setting time of Thai white Portland cement [Master's thesis]. Bangkok: Mahidol University; 2011.
  24. Dentsply Maillefer. $ProRoot^{(R)}$ MTA (Mineral Trioxide Aggregate) Root canal repair material [Internet]. Johnson City (TN): DENTSPLY Tulsa Dental Specialties; 2005 [cited 2016 April 4]. Available from: http://www.dentsplymaillefer.com/wp-content/uploads/2016/10/Dentsply_Maillefer_PROROOT_MTA_0216_DFU_EN.pdf. (updated 2017 Mar 16).
  25. D'Souza RN, Bachman T, Baumgardner KR, Butler WT, Litz M. Characterization of cellular responses involved in reparative dentinogenesis in rat molars. J Dent Res 1995;74:702-709. https://doi.org/10.1177/00220345950740021301
  26. Cotton WR. Bacterial contamination as a factor in healing of pulp exposures. Oral Surg Oral Med Oral Pathol 1974;38:441-450. https://doi.org/10.1016/0030-4220(74)90372-7
  27. Heys DR, Fitzgerald M, Heys RJ, Chiego DJ Jr. Healing of primate dental pulps capped with Teflon. Oral Surg Oral Med Oral Pathol 1990;69:227-237. https://doi.org/10.1016/0030-4220(90)90333-N
  28. Cvek M, Granath L, Cleaton-Jones P, Austin J. Hard tissue barrier formation in pulpotomized monkey teeth capped with cyanoacrylate or calcium hydroxide for 10 and 60 minutes. J Dent Res 1987;66:1166-1174. https://doi.org/10.1177/00220345870660061501
  29. Pereira JC, Stanley HR. Pulp capping: influence of the exposure site on pulp healing--histologic and radiographic study in dogs' pulp. J Endod 1981;7:213-223. https://doi.org/10.1016/S0099-2399(81)80178-1
  30. Tziafas D, Kolokuris I, Alvanou A, Kaidoglou K. Short-term dentinogenic response of dog dental pulp tissue after its induction by demineralized or native dentine, or predentine. Arch Oral Biol 1992;37:119-128. https://doi.org/10.1016/0003-9969(92)90007-U
  31. Camilleri J. Characterization of hydration products of mineral trioxide aggregate. Int Endod J 2008;41:408-417. https://doi.org/10.1111/j.1365-2591.2007.01370.x
  32. Chang SW. Chemical characteristics of mineral trioxide aggregate and its hydration reaction. Restor Dent Endod 2012;37:188-193. https://doi.org/10.5395/rde.2012.37.4.188
  33. Yasuda Y, Ogawa M, Arakawa T, Kadowaki T, Saito T. The effect of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells: an in vitro study. J Endod 2008;34:1057-1060. https://doi.org/10.1016/j.joen.2008.06.007

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