• Journal of Veterinary Science
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• 제21권2호
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• pp.33.1-33.15
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• 2020

Bovine ephemeral fever virus (BEFV) causes bovine ephemeral fever, which can produce considerable economic damage to the cattle industry. However, there is limited experimental evidence regarding the underlying mechanisms of BEFV. Annexin A2 (AnxA2) is a calcium and lipid-conjugated protein that binds phospholipids and the cytoskeleton in a Ca²⁺-dependent manner, and it participates in various cellular functions, including vesicular trafficking, organization of membrane domains, and virus proliferation. The role of the AnxA2 gene during virus infection has not yet been reported. In this study, we observed that AnxA2 gene expression was up-regulated in BHK-21 cells infected with the virus. Additionally, overexpression of the AnxA2 gene promoted the release of mature virus particles, whereas BEFV replication was remarkably inhibited after reducing AnxA2 gene expression by using the small interfering RNA (siRNA). For viral proteins, overexpression of the Matrix (M) gene promotes the release of mature virus particles. Moreover, the AnxA2 protein interaction with the M protein of BEFV was confirmed by GST pull-down and co-immunoprecipitation assays. Experimental results indicate that the C-terminal domain (268-334 aa) of AxnA2 contributes to this interaction. An additional mechanistic study showed that AnxA2 protein interacts with M protein and mediates the localization of the M protein at the plasma membrane. Furthermore, the absence of the AnxA2-V domain could attenuate the effect of AnxA2 on BEFV replication. These findings can contribute to elucidating the regulation of BEFV replication and may have implications for antiviral strategy development.

• 콘크리트학회논문집
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• 제20권2호
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• pp.165-173
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• 2008

철근콘크리트구조물의 거동에서 가장 중요한 요구사항 중의 하나는 철근과 콘크리트 상호간의 합성 거동을 위한 부착 성능의 확보이며, 순환골재 콘크리트를 구조체로 적용하기 위해서는 순환골재와 철근의 부착 거동을 구명하는 것이 매우 중요한 요소로 등장하게 된다. 이러한 분석에 따라 본 연구에서는 순환굵은골재를 사용한 콘크리트와 철근 상호간의 부착거동을 평가하기 위하여 총 36개의 시험체를 제작하여 압축인발 실험을 수행하였다. 연구를 수행함에 있어 실험에 사용된 변수는 0, 30, 60, 100%의 4가지의 순환굵은골재 치환율 및 철근의 배근 방향 및 위치 (상단근, 하단근)로 하였다. 본 연구를 통하여 얻어진 실험 결과를 종합해 보면, 순환굵은골재를 사용한 콘크리트와 철근간의 부착강도는 실험에 사용된 변수인 철근의 배근방향/위치 및 순환굵은골재 치환율에 따라서 그 영향이 상호 다르게 나타나는 것으로 파악되었다. 즉, 수직배근된 철근의 경우는 순환골재의 치환율에 관계없이 상호 유사한 값을 나타내고 있는 반면, 수평 배근된 시험체의 경우는 순환굵은골재 치환율 및 철근의 배근 위치에 영향을 받는 것으로 나타났다. 이는 순환골재의 치환율 변화에 따라 콘크리트 침하량의 차이가 발생되며 추가적으로 상부철근을 통과하지 못한 기포의 영향으로 인해 부착면적이 감소되었기 때문인 것으로 판단되며, 이로 인하여 HU type 시험체의 최대부착응력이 다른 시험체에 비하여 현저히 작은 것으로 나타났다. 따라서 순환골재 콘크리트를 포함하여 철근과 콘크리트의 부착강도 평가를 위한 기존의 규준식에서 철근의 위치에 따른 기여도를 재검토할 필요성이 있을 것으로 사료된다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.31-32
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• 2010

In our previous reports [1-3], electron transport for the switching and memory devices using alkyl thiol-tethered Ru-terpyridine complex compounds with metal-insulator-metal crossbar structure has been presented. On the other hand, among organic memory devices, a memory based on the OFET is attractive because of its nondestructive readout and single transistor applications. Several attempts at nonvolatile organic memories involve electrets, which are chargeable dielectrics. However, these devices still do not sufficiently satisfy the criteria demanded in order to compete with other types of memory devices, and the electrets are generally limited to polymer materials. Until now, there is no report on nonvolatile organic electrets using nano-interfaced organic monomer layer as a dielectric material even though the use of organic monomer materials become important for the development of molecularly interfaced memory and logic elements. Furthermore, to increase a retention time for the nonvolatile organic memory device as well as to understand an intrinsic memory property, a molecular design of the organic materials is also getting important issue. In this presentation, we report on the OFET memory device built on a silicon wafer and based on films of pentacene and a SiO2 gate insulator that are separated by organic molecules which act as a gate dielectric. We proposed push-pull organic molecules (PPOM) containing triarylamine asan electron donating group (EDG), thiophene as a spacer, and malononitrile as an electron withdrawing group (EWG). The PPOM were designed to control charge transport by differences of the dihedral angles induced by a steric hindrance effect of side chainswithin the molecules. Therefore, we expect that these PPOM with potential energy barrier can save the charges which are transported to the nano-interface between the semiconductor and organic molecules used as the dielectrics. Finally, we also expect that the charges can be contributed to the memory capacity of the memory OFET device.[4]

• 한국철도학회논문집
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• 제14권3호
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• pp.240-247
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• 2011

본 연구에서는 콘크리트 궤도 침목 내에 설치되는 인서트에 예기치 못하게 침투된 수분의 결빙압이 앵커볼트의 인발강도에 미치는 영향을 유한요소해석을 통해 고찰하였다. 3차원 유한요소해석모델은 콘크리트 침목의 현장실험 결과, 도면 및 레일체결장치의 제원 실측치를 기반으로 수립되었으며, 비선형구성방정식과 파괴 모델은 측정된 압축강도로부터 CEB-FIP 1990 모델코드를 이용하여 추정하였다. 해석모델의 적정성은 철도기술연구원에서 수행한 현장 인발시험 결과 및 실내시험 결과와의 비교를 통해 확인하였다. 다양한 인자, 즉 결빙위치, 앵커볼트 초기 체결력의 크기 및 콘크리트 압축강도에 따른 해석을 수행하였으며, 그 결과를 제시하였다. 해석결과에 의하면, 매립전내 침투수의 결빙력은 균열손상의 가장 가능성 있는 직접적인 원인 중 하나로 간주될 수 있음을 확인하였다. 또한, 외측매립전의 결빙력이 내측 매립전 보다 작은 것으로 나타났으나 그 차이는 크지 않았다.

• Journal of Ginseng Research
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• 제48권1호
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• pp.40-51
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• 2024

Background: Ginsenoside 20(S)-Rg3 shows promising tumor-suppressive effects in ovarian cancer via inhibiting NF-kB signaling. This study aimed to explore the downstream tumor suppressive mechanisms of ginsenoside Rg3 via this signaling pathway. Materials and methods: A systematical screening was applied to examine the expression profile of 41 kinesin family member genes in ovarian cancer. The regulatory effect of ginsenoside Rg3 on KIF20A expression was studied. In addition, we explored interacting proteins of KIF20A and their molecular regulations in ovarian cancer. RNA-seq data from The Cancer Genome Atlas (TCGA) was used for bioinformatic analysis. Epithelial ovarian cancer cell lines SKOV3 and A2780 were used as in vitro and in vivo cell models. Commercial human ovarian cancer tissue arrays were used for immunohistochemistry staining. Results: KIF20A is a biomarker of poor prognosis among the kinesin genes. It promotes ovarian cancer cell growth in vitro and in vivo. Ginsenoside Rg3 can suppress the transcription of KIF20A. GST pull-down and co-immunoprecipitation (IP) assays confirmed that KIF20A physically interacts with BTRC (β-TrCP1), a substrate recognition subunit for SCF^β-TrCP E3 ubiquitin ligase. In vitro ubiquitination and cycloheximide (CHX) chase assays showed that via interacting with BTRC, KIF20A reduces BTRC-mediated CDC25A poly-ubiquitination and enhances its stability. Ginsenoside Rg3 treatment partly abrogates KIF20A overexpression-induced CDC25A upregulation. Conclusion: This study revealed a novel anti-tumor mechanism of ginsenoside Rg3. It can inhibit KIF20A transcription and promote CDC25A proteasomal degradation in epithelial ovarian cancer.

• Bulletin of the Korean Chemical Society
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• 제23권8호
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• pp.1089-1096
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• 2002

Solvolyses rate constants of trimethylacetyl chloride (2), isobutyryl chloride (3), diphenylacetyl chloride (4) and p-methoxyphenylacetyl chloride (5) in 2,2,2-trifluoroethanol (TFE)-water, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP)-water and TFE-et hanol solvent systems at $10^{\circ}C$ are determined by a conductimetric method. Kinetic solvent isotope effects (KSIE) are reported from additional kinetic data for methanolyses of various substituted acetylchlorides in methanol According to the results of those reactions analyzed in terms of rate-rate profiles,extended Grunwald-Winstein type correlations, application of a third order reaction model based a general base catalyzed (GBC) and KSIE values. Regardless of the kind of neighboring groups (CH3- or Ph-groups) of reaction center, for aqueous fluorinated alcohol systems, solvolyses of 2, 3, 4, and 5 were exposed to the reaction with the same mechanism (a loose SN2 type mechanism by electrophilic solvation) controlled by a similarity of solvation of the transition sate (TS). Whereas, for TFE-ethanol solvent systems, the reactivity depended on whether substituted acetyl chloride have aromatic rings (Ph-) or alkyl groups (CH3-); the solvations by the predominant stoichiometric effect (third order reaction mechanism by GBC and/or by push-pull type) for Ph- groups (4 and 5) and the same solvation effects as those shown in TFE-water solvent systems for CH3- groups (2 and 3) were exhibited Such phenomena can be interpreted as having relevance to the inductive effect ( $\sigmaI)$ of substituted groups; the plot of log (KSIE) vs. ${\sigma}I$ parameter give an acceptable the linear correlation with r = 0.970 (slope = 0.44 $\pm$ 0.06, n = 5).

• 콘크리트학회논문집
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• 제25권2호
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• pp.155-163
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• 2013

이 연구는 도넛형 이방향 중공슬래브에 매입된 이형철근의 부착특성 및 부착강도 산정을 위한 기초적인 연구이다. 도넛형 이방향 중공슬래브의 철근 부착특성 및 부착강도 산정을 위해 pull-out test를 수행하였다. 도넛형 중공형성체는 슬래브 내부에 배치되어 철근과 중공형성체 사이에 내부의 피복이 형성된다. 이러한 내부피복두께는 외부피복두께보다 상대적으로 작은 피복두께를 가지며 중공형상에 따라 $2.5d_b$보다 작은 내부피복두께가 형성되기 때문에 철근의 부착에 영향을 미치게 된다. 또한 중공형성체가 일정 간격으로 떨어져 배치되어 있으므로 인장철근을 감싸고 있는 피복두께의 조건이 철근의 길이방향으로 변하게 된다. 따라서 도넛형 이방향 중공슬래브의 부착특성을 알아보기 위해서 중공형성체 형상에 따라 부착구간을 구분하였다. 구간별로 내부피복두께에 따른 부착응력-슬립 관계를 확인하였으며, 중공형성체 전 구간에 걸친 부착응력분포를 확인하여 철근의 길이방향에 따른 부착응력의 발현정도를 확인하였다. 또한 구간별 부착응력-슬립 관계를 기반으로 하여 도넛형 중공슬래브의 부착강도를 산정할 수 있는 부착강도 산정 방법을 제안하였다.

• 콘크리트학회논문집
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• 제22권1호
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• pp.123-130
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• 2010

순환골재 콘크리트와 철근의 부착 거동 규명은 철근콘크리트 구조물에 순환골재를 적용하기 위해서 매우 중요한 요소이다. 이에 따라 이 연구에서는 콘크리트와 철근 상호간의 부착 거동을 평가하기 압축인발 실험을 수행하였다. 실험에 사용된 변수는 설계압축강도(21, 27, 40 MPa), 순환 굵은골재 치환율(0, 30, 60, 100%) 및 철근의 배근방향 및 위치(상단근, 하단근)로 하였다. 이 연구를 통하여 얻어진 실험 결과를 종합해 보면, V형 시험체의 경우 순환골재 치환율에 관계없이 상호 유사한 값을 나타내고 있으며 H형 시험체의 경우는 철근의 배근 위치에 영향을 받는 것으로 나타났으나 일부 HT형 시험체를 제외하고는 대부분 CEB-FIP 및 ACI 408 기준식에 의한 계산 값을 상회하는 것으로 나타나 재생골재 사용에 따른 성능 저하는 크지 않은 것으로 판단된다. HT형 시험체의 경우 순환골재 치환율 증가에 따라 골재 침하 현상이 크게 발생되며 불리딩 및 레이턴스에 의한 부착면적의 감소로 최대 부착응력이 다른 시험체에 비하여 낮게 나타났다. 전술한 바에 의하면 현행 규준에서 정하는 상부철근의 높이인 300 mm 이하(높이 225 mm)에서도 부착강도의 저하가 발생하며 이에 따라 현재 규준에서 제안하는 철근 위치 보정계수 1.3의 값의 수정과 신뢰성 확보가 필요할 것으로 판단된다.

• 대한약침학회지
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• 제13권2호
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• pp.93-100
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• 2010

Background: Auricular acupressure is one of the traditional health care treatments in oriental medicine. Approximately, 30~40% of the cancer patients have said to be suffering from insomnia and half of them having chronic and severe insomnia at the same time. Insomnia caused cancer patients feel more pain, fatigue, depression and anxiety and it sometimes let the power to have the best of cancer pull down. Objective: To investigate how effective the auricular acupressure treatment to cancer patients suffering from insomnia. Methods: We recruited participants from East-West Cancer Center of Daejeon University. Finally, of the people whose age range from 20 to 75, 12 patients who got less than 40 points from the score of Oh's sleeping score (OSS) were recruited. Single-blind, randomized pilot study was performed. The treatment group received auricular acupressure treatment (AAT) on active points and the control group had received sham acupressure treatment (SAT) for five times. Sleep parameters were checked by using OSS and numeric rating scale (NRS). We checked the scale everytime, both before and after treatment. We analyzed the data statistically by using independent T-test, paired T-test and analysis of variance (ANOVA) test. (p<0.05) Results: Twelve cancer patients participated in this pilot study and there was no significant difference between control and treatment group. Only 7 of them had completed the whole treatment process, 4 patients of AAT group and 3 participants of SAT. The OSS of AAT group had increased from $34.0{\pm}4.3$ to $39.5{\pm}3.1$ and that of SAT group had increased from $38.3{\pm}3.5$ to $40.0{\pm}0.0$. There was no significant difference between them. The NRS of AAT group had increased from $6.3{\pm}2.9$, $04.8{\pm}2.1$ and that of SAT group had increased from $7.0{\pm}1.0$ to $5.0{\pm}2.6$. No significant difference was observed between them. Conclusion: Although both groups did not show significant differences, most of the experimental participants showed increasing OSS and NRS after treatments. Significant participants' number will be needed in the next study.

• Steel and Composite Structures
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• 제35권4호
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• pp.463-474
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• 2020

In this paper, the influence of adding multi-walled carbon nanotube (MWCNT) on the pull behavior of steel and GFRP bars in ultra-high-performance concrete (UHPC) was examined experimentally and numerically. For numerical analysis, 3D nonlinear finite element modeling (FEM) with the help of ABAQUS software was used. Mechanical properties of the specimens, including Young's modulus, tensile strength and compressive strength, were extracted from the experimental results of the tests performed on standard cube specimens and for different values of weight percent of MWCNTs. In order to consider more realistic assumptions, the bond between concrete and bar was simulated using adhesive surfaces and Cohesive Zone Model (CZM), whose parameters were obtained by calibrating the results of the finite element model with the experimental results of pullout tests. The accuracy of the results of the finite element model was proved with conducting the pullout experimental test which showed high accuracy of the proposed model. Then, the effect of different parameters such as the material of bar, the diameter of the bar, as well as the weight percent of MWCNT on the bond behavior of bar and UHPC were studied. The results suggest that modifying UHPC with MWCNT improves bond strength between concrete and bar. In MWCNT per 0.01 and 0.3 wt% of MWCNT, the maximum pullout strength of steel bar with a diameter of 16 mm increased by 52.5% and 58.7% compared to the control specimen (UHPC without nanoparticle). Also, this increase in GFRP bars with a diameter of 16 mm was 34.3% and 45%.