• 한국동물위생학회지
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• 제31권1호
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• pp.1-16
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• 2008

Salmonella infections cause the disease in pigs but also some zoonotic Salmonella serotypes can be transmitted to human through swine products, resulting in food poisoning. The objective of this study was to investigate the bacteriological prevalence and detection of invA gene using Salmonella specific polymerase chain reaction (PCR), the epidemiological characteristics related to Salmonella strains cultured from pig samples in Gangwon areas using serotyping, random amplified polymorphic DNA (RAPD) and pulsed field gel electrophoresis (PFGE) methods. During the period of November 2001 through April 2002, 1,174 ileocecal lymph node were collected from the slaughtered pigs raised in 38 farms located in Gangwon province. The samples were submerged in boiling water and macerated in saline and lymph node homogenates were inoculated into Tetrathionate broth with iodine (TTB, Difco, 0.5% iodine was added) for enrichment growth. Then additional tests were performed using several mediums, and suspects were identified by API 20E kit (BioMerieux) and PCR. Of total 1,174 samples from 38 farms, 44 (3.7%) were isolated as Salmonella spp from 13 farms (34.2%). Of 44 isolates, 31 were in Yangyang region, followed by 9 in Goseong, 2 in both Gangneung and Sokcho. However, there was no difference in regional isolation frequency. All isolates have a 521bp amplified product in Salmonella specific PCR with primer invA which encodes in proteins for invasion of epithelial cells. Of 44 recovered serotypes, 23 (52.3%) were S Eingedi, 10 (22.7%) S Schwarzengrund, 9 (20.5%) S Typhimurium, and 2 (4.5%) S Mbandaka. In RAPD analysis, there appeared to be unique bands distinguishing each serotype, although similarities exist between the different serotypes. Four serotypes of 44 Salmonella isolates appeared to fall into 14 different RAPD types. In PFGE analysis, 9 S Typhimurium were tested with XbaI enzyme and SpeI enzyme. The combination of results obtained with two enzymes subdivided the 9 S Typhimurium into 4 PFGE types.

• 대한간호학회지
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• 제23권3호
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• pp.377-398
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• 1993

A survey of infant practices was conducted to provide information on which to base planned nursing interventions. The subjects were a convenience sample of 168 mothers visiting out - patients departments of five general hospitals and one public health center in Seoul for immunizations or treatment for common colds for their infants from two to 12 months of age. Data collection was carried out from July 8th to September 30th, 1991 using a questionnaire of 84 questions, 31 on the type of feeding, 22 on supplementary feeding and 21 on demographic infer mation. The results are summarized as follows : 1) The type of feeding was primarily artificial feed-ing (63.1%), followed by mixed feeding (22%) and breast feeding (14.9%) of the 59 mothers expecting during pregnancy to breast feed, 54. 2% changed artificial feeding and 30.5% went on to breast feed as they had expected. For mothers expecting to continue breast feeding over seven months. only one infant was being breast fed for over seven months. 2) For the 106 mothers using artificial feeding, 70. 8% hed attempted breast feeding, 64% of them for less than a month. Breast milk had been suppressed by for medication (38.7%) : 34.9% had used no specia] means. 3) The major reasons for replacing breast feeding with artificial feeding were the infants' demand for more milk (47.2% ) and insufficient supply of breast milk (49.3％). 4) Most mixed feeding was started at the age of one to three months (59.5%). Only 34.4% gave an artificial feeding after breast feeding : most (46%) alternated breast feeding with artificial feeding. On the whole, the motive for mixed feeding was the lack of breast milk (70.3%). 5) Many mothers (81.8%) were adding vitamin or mineral supplements to artificial milk and 51.5% were adding something to faciliate digestion. As for the method of sterilizing milk bottles and nipples, 56% had sterilized them together in boiling water from the beginning : 27% were just washing the bottles after boiling only once initially when measuring artificial milk powder, 31. 5% of the mothers over filled the measuring spoon rather than to the level. 6) The mother's occupation was related to her way of feeding. Mothers at home full time did more breast feeding than mothers employed outside the home. (x²=5.72, p=〈0.05). 7) Most mothers began supplementary food, from three to four months (48.8%) : 11.2% began later than seven months. Supplementary food was given between milk feedings by 67.2% of the mothers : 19.2% gave it before a milk feeding. Some mothers(26.4%) made their own supplementary food : 19.2% used ready - made supplementary food products for convenience. Recommendations for nursing interventions included : 1. Prenatal education about the advantages of breast feeding and breast care, and home visits after delivery for counselling related to breast feeding. Correct preparation of artificial feeding methods need to be taught in both pre & postnatal periods. In addition, specific education about supplementary feeding needed. 2. Further research is indicated about the Perceived lack of supply of breast milk and about the effectiveness of nursing interventions to Promote breast feeding.

• Korean Chemical Engineering Research
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• 제50권2호
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• pp.310-316
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• 2012

활성탄소 분말과 탄소필터를 이용하여 실리콘 폐슬러리에서 회수한 폴리에틸렌글리콜(polyethylene glycol, PEG) 폐액의 색도 개선연구를 수행하였다. 활성탄소의 사용량, 흡착온도 및 흡착시간 변화에 따른 색도 변화를 관찰하였다. 탄소필터를 사용할 경우 충진된 활성탄소의 재생에 따른 성능 저하 여부를 검정하였다. 활성탄소의 표면적은 색도 개선에 큰 영향을 주지 않았다. 흡착제 함량에 따른 색도 변화 결과로부터 흡착제의 최적 사용량은 100~150 mg-C/g-PEG이였다. 흡착제 양을 고정하고 흡착 온도를 상온에서 $100^{\circ}C$까지 변화시키면서 PEG의 APHA(American Public Health Association) 색 값을 관찰한 결과 폐 PEG의 색도 개선을 위해서는 $40{\sim}50^{\circ}C$ 온도에서 운전하는 것이 가장 효과적이었다. 활성탄소필터의 운전 조건별 APHA 특성을 관찰하였고 APHA=53인 폐 PEG를 $APHA{\leq}10$로 정제 가능함을 확인하였다. 흡착에 사용된 활성탄소필터는 증류수를 순환시켜 세척함으로써 큰 성능 저하 없이 효과적으로 재생됨을 확인하였다.

• 한국환경보건학회지
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• 제35권1호
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• pp.21-35
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• 2009

The Asian dust storms which originated in the deserts of Mongolia and China transported particles to Korea and led to a high concentration of atmospheric particulate matters (PM) of more than $1000{\mu}g/m^3$ throughout the country in the spring, of 2007. Public concern, in Korea, about the possible adverse effects of these dust events has increased, as these dust storms can contain various air pollutants emitted from heavily industrialized eastern China. The objectives of this study were to understand the concentration characteristics of PM as a function of particle size between the Asian dust storm episodes and non-Asian dust period and to consider the mass size distribution of PM in the Asian dust storms and their water soluble ion species on the potential, possible effects on deposition levels in the three regions (nasopharyngeal, tracheobronchial, and alveolar) of the human respiratory system. The size distribution of PM mass concentration during the Asian dust storms showed a peak in the coarse particle region due to the long-range transport of soil particles from the deserts of Mongolia and China, which was identified by HYSPLIT-4 model for backward trajectory analysis of air arriving in the sampling site of Iksan. During the non-Asian dust period, there were two different types in PM size distribution: bimodal distribution when low concentrations of $PM_{2.5}$ were observed, while unimodal distribution having a peak in fine particle region when high concentrations of $PM_{2.5}$ were showed. This unimodal distribution with high concentrations of fine particulate and secondary air pollutants such as ${SO_4}^{2-}$, ${NO_3}^-$, ${NH_4}^+$ was found to be due to the long-range transport of air pollutants from industrialized eastern China. During the Asian dust storms, the mean concentrations of PM that can be deposited in the nasopharyngeal, tracheobronchial, and alveolar region were $128.8{\mu}g/m^3$, $216.5{\mu}g/m^3$, and $89.6{\mu}g/m^3$, respectively. During the non-Asian dust period, the mean concentrations of PM that can be deposited in the nasopharyngeal, tracheobronchial, and alveolar region were $8.4{\mu}g/m^3$, $9.5{\mu}g/m^3$ and $38.5{\mu}g/m^3$, respectively.

• Spatial Information Research
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• 제14권3호
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• pp.349-361
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• 2006

국내외적으로 농촌 경관에 대한 관심과 함께 경관에 대한 관리 및 보전에 노력을 기울이고 있다. 천혜의 자연조건과 함께 아름다운 농촌경관을 유지하고 있는 제주도에서도 경관의 중요성을 인식하여 제주국제자유도시특별법 및 동법 시행조례로서 경관관리를 하고 있다. 그러나 이러한 경관관리에 대한 노력에도 무분별한 건축물의 난립 등으로 인하여 경관훼손이 가해져 체계적인 관리방안이 시급한 처지이다. 이러한 농촌경관 가운데에서도 대표적인 경관을 구성하는 돌담의 훼손이 심각히 진행되어지고 있지만 현재의 경관관리는 조망지역의 가시권에 대해서만 초점을 맞추고 있고 중요한 경관요소에 대해서는 경관평가 항목에 포함되어 있지 않아 돌담 경관의 훼손에 대해 아무런 조치를 취할 수 없는 실정에 있다. 이러한 배경 하에 본 연구는 제주도 돌담이 경관 요소로서 가지고 있는 중요한 내재적 가치를 정립하였고 또한 기존의 가시권 중심의 조망지점 경관분석에서 훼손 가능성을 경관분석에 적용함으로서 보전측면을 강화한 경관평가를 제안하였다. 또한 GIS를 활용하여 돌담 경관 실태와 훼손정도를 추정하여 GIS 기술의 활용여부도 검토하였다. 이 연구를 위해 제주도의 대표성을 지니는 한경, 애월, 신촌 등 여섯 군데의 농촌지역을 사례지역으로 정하였으며 이 지역에 대한 경관분석을 실시하였다.

• 치위생과학회지
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• 제6권2호
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• pp.93-99
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• 2006

본 연구는 구강위생용품의 사용 실태를 알아보기 위하여 S시 D중공업의 근로자를 대상으로 가정에서 가장 관심을 가지고 관리하는 구강병, 잇솔의 사용실태, 불소치약의 사용여부, 구강위생보조용품의 사용실태, 구강위생보조용품의 사용 및 비사용의 동기를 조사 분석한 결과 다음과 같은 결론을 얻었다. 1. 가정에서 가장 관심을 가지고 관리하는 구강병은 치아우식증(62.4%)으로 나타났다. 2. 칫솔 사용실태는 중간 크기를(79.6%) 가장 많이 사용하였다. 3. 잇솔의 강모 모양은 일반 수평형(73.5%)을 가장 많이 사용하였다. 4. 불소치약 사용여부는 아무 종류나 사용한다(73.5%)가 가장 많았다. 5. 구강위생용품의 인지도에 대해서는 전동잇솔이(77.0%)로 가장 높으며 구강위생보조 용품에 대한 인지도는 이쑤시개(89.4%)와 양치용액(84.5%)을 가장 많이 알고 있었다. 구강위생용품의 사용여부는 전동잇솔(13.3%), 치주환자용 잇솔(2.7%), 순서로 나타나며, 구강위생보조용품에서는 이쑤시개(58.4%), 양치용액(41.2%)의 순이었다. 사용효과는 틀니잇솔(50.0%), 전동잇솔(43.3%)로 나타났으며, 구강위생보조용 품에서 효과 여부는 물사출기(80.0%), 치실(75.8%)이 매우 높았다. 6. 구강위생보조용품을 사용하게 된 동기는 TV나 광고 홍보물을 보고 독자적인 판단으로 사용하는 경우가 (57.6%) 높게 나타났으며, 구강위생보조용품을 사용하지 않는 이유로는 자신에게 적절한 보조용품을 모르기 때문에 사용하지 못한다는 의견이 매우 높게 (57.4%)나타났다.

• 한국환경교육학회지:환경교육
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• 제5권1호
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• pp.112-137
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• 1993

As the concern with the creation of environmental problems and regulations about them becomes internationally growing, environmental cooperations with countries concerned are emerging as the first item on the political agenda. In case of South Korea, cooperations with countries in the Asian region is needed urgently. In particular, cooperations with North Korea will be an important factor to reunify the Korean peninsula as well as to solve environmental problems in South Korea. Accordingly, it is necessary to seek feasible alternatives and possibilities of coping with international environment in South and North Korea. This study is limited to literature study based on collecting materials and the testimony of former residents in North Korea. The period of study ranges from May to December in 1992. The research findings show the following environmental problems and alternatives : It is recognized in South Korea that the creation of environmental problems is product of economic development. Currently, the Korean government makes an effort to revise environmental administration and law, to encourage environmental education, and to proceed with environmental technology policies by establishing environmental policies together with economic development plans. North Korea ascribes the cause of environmental problems to colonial exploitation by Japan and the U.S. Environmental pollutants in North Korea is relatively less than South Korea, provisions such as environmental conservation movement, environmental education, and environmental administration and law are not well organized in the country. However, North korea will speed up its economic development to solve economic crises. Therefore, it is expected that industrial wastes, corrosion of nature, and water pollution will result in serious environmental problems. The fields expected to cooperate with South and North Korea are in the following : South Korea may adopt the model of the former East and West Germany and proceed to cooperate with North Korea in public sector as well as private sector. The possible programs are such as academic exchange to conserve environment, joint research in the area of the Demilitarized Zone, conservation of ecosystems around Imjin and Pukhan Rivers, South Korea's support of advanced environmental technology and industrial and financial aid to North Korea. Conflicts between countries concerned in the North East Asian region will be caused by the responsibilities of pollutions because of the seashore development of the Yellow Sea by South Korea and increasing seashore industrial complexes around the Yellow Sea in China, Therefore, it is desirable that the two countries will participate in organizations related to environment. Joint projects between South and North Korea will be as follows : 1. Construction of long-range monitoring system : 2. Investigation of the state of pollutions around the Yellow Sea and alternatives : 3. Construction of joint monitoring system to regulate the movement of pollutants : 4. Cooperation of environmental technology and exchange of information ; and 5. Support of Cooperation of environment in private sector. Efforts to cooperate with North korea is expected to overcome differences between the two countries as well as to encourage unification of the korean peninsula, which will lead to reducing the cost of environmental recovery. These efforts will also contribute to the maintenance for peace and stability on the korean peninsula as well as in the North East Asian region.

• 약학회지
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• 제55권5호
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• pp.411-418
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• 2011

The dissolution test method and an analytical procedure by HPLC were developed and validated for nafronyl oxalate capsules and tramadol hydrochloride capsules. These drugs were not yet characterized by the dissolution specifications in the Korean Pharmaceutical Codex. So, with each reference and test drugs, we did the preliminary and standard experiments based on the Korean Pharmacopeia Guideline of dissolution testing for solid oral dosage forms. The dissolution test for nafronyl oxalate capsules was carried out under sink conditions as follows: dissolution medium phosphate buffer pH 6.8, paddle rotation speed 100 rpm and vessel volume 900 ml. More than 80% of its label amount was released within 30 min in this method. Also the dissolution test for tramadol hydrochloride capsules was carried out under sink conditions as follows: dissolution medium water, paddle rotation speed 50 rpm and vessel volume 900 ml. More than 90% of its label amount was released within 15 min in this method. The dissolution samples were analyzed with a validated HPLC analytical procedure. The analytical methodology showed acceptable values in terms of specificity, linearity, precision and accuracy. The dissolution test methods described above were adequate for the purpose and may be proposed as a pharmacopeial standard to assess the performance of nafronyl oxalate capsules and tramadol hydrochloride capsules. Furthermore, the outcomes of this study were expected to help create an environment where safe and high quality drugs would be distributed on the domestic market making contributions to advancing public health.

• 한국식품영양과학회지
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• 제41권11호
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• pp.1571-1576
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• 2012

가공용으로 공급되는 국내산 정부미와 수입산 정부미의 품질 특성을 조사하고 빵이나 국수의 원료로 사용하기 위하여 제빵 및 제면성을 시험하였다. 두 가지 정부미 모두 가루의 백색도가 낮고 황색도가 증가되어 저장된 쌀의 색의 특성이 관찰되었고 아밀로오스 함량이 13% 이하로 국수나 빵을 만드는데 적합한 고 아밀로오스(22% 이상) 쌀로 사용하기에는 부적합하였다. 낟알의 경도는 수입산 정부미가 가장 단단하였지만 제분된 가루의 손상 전분 함량과 수분 흡수율은 시료 간에 유의적인 차이가 없었다. 수입산 정부미의 호화 점도가 높고 국내산 정부미의 호화 점도가 상대적으로 낮았으나 두 가지 모두 저장되지 않은 가공용 쌀 Goamibyeo에 비하여 높은 점도를 나타내어 일반적인 저장 쌀의 호화 특성이 관찰되었다. 그러나 저장기간에 따라서 감소된다고 알려진 breakdown viscosity는 정부미가 오히려 높은데 이는 저장에 따른 효과보다 정부미 자체 전분의 특성에 기인한 것으로 예측된다. 두 가지 정부미 모두 빵의 발효와 굽기과정까지는 우수한 제빵성이 관찰되었지만 굽기 후 식히는 과정에서 빵의 모양이 유지되지 못하고 가라앉아서 100% 쌀 빵을 만들기에 부적합하였다. 100% 쌀로 만든 국수의 조리 손실률은 정부미가 Goamibyeo보다 적었지만 국수의 탄성이 적고 신장성이 커서 무른 특성이 나타났다. 따라서 가공용으로 공급되는 국내산 및 수입산 정부미를 빵이나 국수 등의 원료로 이용하려면 밀가루 또는 제면 제빵성이 우수한 곡류를 혼합하여 반죽의 물성을 개량한 후 사용하는 것이 유리할 것으로 판단된다.

• 한국미생물·생명공학회지
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• 제43권4호
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• pp.396-400
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• 2015

Enterobacteriaceae에 속하는 세균들은 보건학적 문제를 발생시키기도 하며 항생제 내성 병원균의 발생으로 인하여 자연환경 중의 항생제 내성균에 분포에 대한 관심이 증대되고 있다. 따라서 배양법을 이용하여 국내 5개의 하천시료로부터 그람 음성 Enterobacteriaceae의 종 다양성을 조사하였으며 분리된 세균에 대한 다제내성을 항생제디스크 확산법으로 분석하였다. 그 결과, 분리된 그람 음성 Enterobacteriaceae는 Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Pantoea, Plesiomonas, Raoultella, Shigella로 동정되었으며 49%가 Enterobacter로서 우점종으로 분석되었다. 또한 이들 세균들의 78.3%가 조사된 13종의 항생제 중에서 7종 이상에 내성을 보였으며 이것은 국내 자연하천환경에 높은 비율의 다제내성균이 서식한다는 것을 제시한다.