• Journal of Environmental Health Sciences
• /
• v.24 no.2
• /
• pp.100-103
• /
• 1998

A survey on the incidence of metacercariae infection of Clonorchis sinensis in the fresh water fish, Pseudorasbora parva which is well known as the second intermediate host for this fluke was carried out in the upper stream of Phoung-tack stream, Kyungki province during the period of October to November 1997. Forty fish in P. parva were collected by netting, or using the bait in transparent bowls from the two different districts located in Osung-myun, Phoungtack-gun and transported to the laboratory. The body length and weight were measured. They were from 5.0 to 7.6 cm in length and 1.0 to 5.0 g in weight. A total of 40 P. parva were divided 3 groups by the size (70-79, 60-69 and 50-59 mm) and were digested by artificial digestion with pepsin-Hcl solution in a 36$\circ$C incubator and then examined the infection density of metacercariae of the fresh water fish under a binocular dissecting microscope. The incidence of metacercariae infection in P. parva was 406 of 40 examined and the metacercariae detection rate showed 77.5%. The average number of metacercariae detected in P. parva was 10.2. The results of the survey indicate that Clonorchis sinensis infection may occur by consuming raw fresh water fish caught from the upper stream of Phoungtackho, Kyungki province.

• Parasites, Hosts and Diseases
• /
• v.46 no.2
• /
• pp.83-86
• /
• 2008

Holostephanus metorchis (Digenea: Cyathocotylidae) is a parasite of birds, transmitted by freshwater fishes. H. metorchis adults were recovered from chicks experimentally infected with metacercariae collected from freshwater fishes, Pseudorasbora parva. The metacercariae were oval, surrounded with thick fibrous capsules. In adult flukes, the holdfast organ occupied the ventral concavity, and the anterior testis did not reach the level of the ventral sucker. Based on these morphological characteristics, these flukes were identified as H. metorchis.

• Fisheries and Aquatic Sciences
• /
• v.18 no.1
• /
• pp.99-107
• /
• 2015

A natural hybrid of a probable intergeneric mating between the striped shiner Pungtungia herzi and the stone morocco Pseudorasbora parva (Cypriniformes: Cyprinidae) was captured in the Geumho River, a tributary of the Nakdong River basin in Korea. Morphological characters and DNA sequences were analyzed to verify its hybrid state and identify the parentage of its parent species. The hybrid exhibited a phenotypic intermediacy between the two parent species in the number of vertebrae and the mouth shape. Out of 1,488 base pair (bp) positions of the nuclear recombination activating gene 1 gene (rag1), which has a biparental mode of inheritance, 41-bp substitutions were detected between the two parent species, whereas an electropherogram of the hybrid displayed polymorphic double peaks at all of the base positions, along with one additional one, strongly indicating its hybrid state. Meanwhile, sequence comparison of the mitochondrial cytochrome b gene (mt-cyb) (1,140 bp), which has a maternal mode of inheritance, showed only 5-22-bp differences (97.6-99.5% identities) between the hybrid and Pu. herzi, but as many as 158-168-bp differences (85.2-86.1% identities) between the hybrid and Ps. parva, clearly indicating Pu. herzi as the maternal species. Thus, combined analyses of independent data sets (i.e., morphology and nuclear and mitochondrial DNA sequences) offered convincing evidence for the hybrid state of a naturally occurring hybrid resulting from intergeneric mating between a female Pu. herzi and a male Ps. parva.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2005.09a
• /
• pp.456-457
• /
• 2005

• Journal of fish pathology
• /
• v.25 no.2
• /
• pp.85-93
• /
• 2012

Many metacercariae in the musculature of southern top mouthed minnow (Pseudorasbora parva) cultured at a farm in Chonbuk Province. The excysted metacercariae looked yellowish white and were $4.02{\pm}1.80$ mm (1.66-5.81) in length and $1.27{\pm}0.27$ mm (1.01-1.64) in width for acetocarmin-stained specimens. The oral sucker measuring $0.18{\pm}0.05$ mm (0.11-0.24) in length and $0.23{\pm}0.07$ mm (0.15-0.33) in width, was located at the anterior end of the body. The ventral sucker situated at the fourth third of the body were measured $0.47{\pm}0.08$ mm (0.35-0.53) in length and $0.55{\pm}0.09$ mm (0.43-0.63) in width. The tongue-shaped metacercariae were identified as Clinostomum complanatum on the basis of morphological characteristics. The metacercaria was also confirmed to be C. complanatum using PCR reaction with C. complanatum-specific primer sets, Trem 18S F and Ccom670 R. No histopathological changes in the infected fish were observed except necrosis and exfoliation of epidermis in the skin and atrophy of muscle fibers.

• Korean Journal of Ichthyology
• /
• v.27 no.1
• /
• pp.26-32
• /
• 2015

The histological structure of the heart in Pseudorasbora parva was investigated by light microscope. The heart consisted of four consecutive chambers, the sinus venosus, atrium, ventricle and bulbus arteriosus. The wall of the sinus venosus was divided into endocardium, myocardium and epicardium, and the walls of the atrium and ventricle were divided into endocardium, subendocardium, myocardium, subepicardium and epicardium, and the wall of the bulbus arteriosus was divided into endocardium, subendocardium (ridge tissue), middle layer, subepicardium and epicardium. The valves were observed in the sinoatrial, artrioventricular and bulboventricular junctions. The sinus venosus wall was mostly made up of collagen. The rings of tissue were observed at the sinoatrial junction. The atrium was composed of a spongy trabeculate myocardium surrounded by an external rim of thin myocardium, and collagens were distributed in the subepicardium and trabeculae. The ventricle was a spongy myocardium with vessels in subepicardium. In the subepicardium and trabeculae of the ventricle, collagens were distributed. In the bulbus arteriosus, the diameter and length of the ridges were differed. The endocardial cells were convex and the non-clustered subendocardial cells showed irregular shapes. The cells of the middle layer were arranged into incomplete layers that showed different orientations. The subepicardium was formed by cells of different morphology. Collagens and elastins were demonstrated in the subendocardium, middle layer and subepicardium of the bulbus arteriosus. The epicardium was a single layer composed of flattened cells.

• Korean Journal of Environment and Ecology
• /
• v.26 no.6
• /
• pp.884-891
• /
• 2012

The characteristic of fish fauna and distribution by habitat type in the Yanghwa stream were investigated from August, 2008 to June, 2009. During the surveyed period, 30 species belonging 8 families were collected. And there were 10 Korea endemic species(33.3%), including Rhodeus uyekii et and so on. Dominant species was Rhodeus notatus, and subdominant species was Pseudorasbora parva. Dominant species according to habitat type was Pseudorasbora parva in Dam type pool and Channel conected pool, Pseudogobio esocinus(Run), Zacco platypus(Riffle), Rhodeus notatus(Side channel and Substrate type pool), Squalidus gracilis majimae(Meander type pool), and Carassius auratus(Channel unconnected pool), respectively. As a results of community analysis in the Yanghwa Stream, diversity, richness, and abundance indices showed relatively high values, indicating that studied stream have relatively stable community structure. Moreover, cluster and principal component analysis were divided by two groups(lotic and lentic habitats), suggest that species and individuals were different among habitat types.

• Biomedical Science Letters
• /
• v.8 no.1
• /
• pp.7-11
• /
• 2002

This study was performed to investigate the infection status with Clonorchis sinensis metacercariae in Pseudorasbora parva from a tributary stream of Naktonggang (River) in Kangso-gu, Pusan. A total of 325 P. parva was collected in 4 sites using fish traps. They were transferred in the laboratory, measured the length and examined by artificial digestion method. In the site I, a total of 72 (80.0%) out of 90 fish examined was infected with 1~665 (112 in average) metacercariae of C. sinensis. The average number of metacercariae infected by the size of fish was 146 in the group of over 8 cm, 61 in 7.0~7.9 cm and 86 in below 6.9 cm. In the site II, a total of 50 (83.3%) out of 60 fish examined was infected with 1~178 (31 in average) metacercariae. The average number of metacercariae was 40 in the group of over 8 cm 35 in 7.0~7.9 cm, 23 in 6.0~6.9 cm and 25 in below 5.9 cm. In the site III, a total of 68 (97.1%) out of 70 fish examined was infected with 1~2,662 (508 in average) metacercariae. The average number of metacercariae was 679 in the group of over 7.0 cm, 444 in 6.0~6.9 cm and 426 in below 5.9 cm. In the site IV, a total of 103 (98.1%) out of 105 fish examined was infected with 1~1,536 (416 in average) metacercariae. The average number of metacercariae was 532 in the group of over 7 cm, 353 in 6.0~6.9 cm and 262 in below 5.9 cm. From the above results, it is confirmed that there are wide differences in the infection status of C. sinensis metacercariae in P. paeva according to investigation sites, and P. parva collected from the lower stream are more heavily infected than those from the upper stream.

• Journal of Korean Society on Water Environment
• /
• v.21 no.1
• /
• pp.79-83
• /
• 2005

Foraging behaviour of false dace, Pseudorasbora parva, was investigated in water flowing at various velocities with the existence of a cavity for rest. The pursuit comprised three succeeding processes such as, approaching, chasing and attacking. Angles between the fish body and the water flow direction and swimming speeds increased in the latter stages of approaching, chasing and attacking. All pursuit angles, swimming speeds and distances increased with flow velocity and peaked at the flow velocity of 7 cm/sec. At higher velocities, however, the fish avoided the use of much energy against the large drag force. The probability of capture and the feeding rate steadily decreased with increasing flow velocity. Under the fast flow, the fish adjusted their swimming speed to get the optimum velocity relative to the flowing water for the energetic budget. Fish spent more time in the cavity as flow velocity increased to avoid the energy expenditure necessitated by the high velocity.

• Microbiology and Biotechnology Letters
• /
• v.3 no.2
• /
• pp.63-68
• /
• 1975

A biological active substance was isolated from the cultured medium of Streptomyces sp. and its biochemical characteristics were investigated. Isolation process of the substance was as follows; the pH of filterate of the cultured medium was adjusted to 3.0 with N-hydrochloric acid and saturated with sodium chloride, then chloroform was added to this filterate in one fifth portions and stirred vigorously. After extracting the active substance with chloroform in 3 stages, the chloroform layer combined and evaporatea after dehydrating with sodium sulfate. The substance was found to be to be toxic to various fresh water fishes; the lethal dose for an average size Pseudorasbora parva T. et. S. was 50ug per ml. In the acidic condition, the toxicity of the substance remained fora long time, while in the alkaline state, the toxicity was decreased very fast. This substance was found to be stable to organic solvents, but labile to heat treatment. The maximal revival time of Pseudorasbora parva T. et. S. was about 20 minutes in 25 ug/ml of the substance solution.