• 미생물학회지
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• 제25권1호
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• pp.1-8
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• 1987

An extracellular $\beta$-1, 3-glucanase from Pseudomonas stutzeri KF 13 was purified about 390 with 26% recovery. The purified enzyme revealed a single band by polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis. The enzyme was stable in a pH 6.0 to 9.0, and relatively thermostable. The optimal pH and temperature on the enzyme activity were found to be 5.8 and 45.deg.C, respectively. The activation energy was calculated to be 16,130 cal per mole. The Km value for laminarin was found to be 3ng per ml and the molecular weight was determined to be 28,000 by gel filtration and 26,000 daltons by SDS-acrylamide gel electrophoresis. The enzyme was inhibited by 1.0mM of $Hg^{2+}$, and strongly inhibited by 1.0mM of p-chloromercuribenzoic acid.

• 미생물학회지
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• 제24권3호
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• pp.295-301
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• 1986

The bacteria, which were capable of producing ${\beta}-1$, 3-glucanase inducibly by utilizing cell wall of Aspergillus fumigatus as a sole carbon source, were isolated from soil in the campus of Kyungpook National University. Among them, the strain which produced the enzyme excellently was selected and identified to be Pseudomonas stutzeri KF 13 by morphological, cultural and physiological examination. The optimal conditions for the enzyme production from Pseudomonas stutzeri KF 13 were investigated. the enzyme production was reached maximum state shen the broth cultured for 72hr at $30^{\circ}C$. And the enzyme showed the highest activity in the medium containing 3.5% cell wall as an inducer, 15% yeast autolysate as a nitrogen source and 0.05% $MnSO_4$ at pH 7.5.

• 미생물학회지
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• 제25권1호
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• pp.9-16
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• 1987

분리한 살리실산 자화세균 중 플라스미드블 갖는 세 균주를 션별하였다. 세 균주, KU801(pKUs, pKUS) , KUS03(pKU6 pKU9), KUS06(pKU7, pKU10)는 각각 두 개씩의 플라스미드플 가지고 있음이 전기갱동에 의해 밝혀졌고, Pseudomoηas putida로 동정되었다. 세 문주들은 모두 암피실린, 터l트라사이클린, 클로람페니콜등의 항생제에 대하여 내성을 지니며, 조사 된 방화족과 지방족 탄화수소들 중 삼리실산과 그의 중간 대사물인 카터1콜만을 이용하있다. 큰 분자량의 플라스비드(pKUS, p pKU6, pKU7)는 마이로마이신 C로 처리하였을때 큐어되며 그 빈도는 각각 0.40%, l,67%, 0.7S% 이었다. 큐어된 균주는 상리실산을 분해하지 못하였으나, 여전히 야생균주와 동일한 항생제 내성을 가지고 있었다. 살리실산 분해에 관여하는 유전자가 그들 플라스미드에 있는 것으로 판명되었다. pKU5와 pKU6의 분자량은 103, SMd, pKU7의 분자량은 101Md으로 측정되었다. SAL 플라스미드인 pKU5, pKU6, pKU7은 접합에 의해 P.putida와 P.aeruginosa로는 전달되었으나, E. coli에서는 발현되지 아니하였다.