• Title/Summary/Keyword: Pseudomonas sp. P7014

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Identification of Auxin from Pseudomonas sp. P7014 for the Rapid Growth of Pleurotus eryngii Mycelium (큰느타리버섯 균사체의 생육촉진을 위한 Pseudomonas sp. P7014으로부터 옥신 확인)

  • Kang, Young Min;Cho, Kye Man
    • Korean Journal of Microbiology
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    • v.50 no.1
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    • pp.15-21
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    • 2014
  • The promoting effect of Pseudomonas sp. P7014 on the mycelia growth of Pleurotus eryngii was investigated. An ethyl acetate fraction (F5) from the culture supernatant of the bacteria was confirmed to contain the growth promoting compound (GPC). The GPC was identified to be indole acetic acid (IAA) by TLC, HPLC, MS/MS, and NMR analyses. P. eryngii mycelia grew rapidly both on PDA and in PDB after the treatment of GPC. The promoting concentration of GPC was as low as 1.0 nM. Tryptophan, the aminated form of IAA, was confirmed to be the precursor of IAA. These results suggested that bacterial secreted compound was IAA and plays an important role in promoting growth of mushroom mycelia.