• Title/Summary/Keyword: Pseudomonas sp. P2

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Characterization of the pcbD Gene Encoding 2-Hydroxy-6-Ox0-6-Phenylgexa-2,4-Dienoate Hydrolase from Pseudomonas sp. P20

  • Lim, Jong-Chul;Lee, Jeong-Rai;Lim, Jai-Yun;Min, Kyung-Rak;Kim, Chi-Kyung;Ki, Young-Soo
    • Journal of Microbiology and Biotechnology
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    • v.10 no.2
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    • pp.258-263
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    • 2000
  • 2-Hydroxy-6-oxo-6phenylhexa-2,4-dienoate (HOPDA) hydrolase catalyzes the hydrolytic cleavage of HOPDA to bemzpate and 2-hydroxypenta-2, 4-dienoate (HPD) during microbial catabolism of biphenyl and polychlorinated biphenyls. A HOPDA hydrolase gene (pcbD) was isolated from the genomic library of Pseudomonas sp. P20 and designated as pCNUO1201; a 7.5-kb XbaI DNA fragment from Pseudomonas sp. P20 was inserted into the pBluescript SK(+) XbaI site. E. coli HB101 harboring pCNU1201 exhibited HOPDA hydrolase activity. The open reading frame (ORF) corresponding to the pcbD gene consisted of 855 base pairs with an ATG initiation codon and a TGA termination codon. The ORF was preceded by a rebosome-binding sequence of 5'-TGGAGC-3' and its G+C content was 55 mol%. The pcbD gene of Pseudomonas sp. P20 was located immedeately downstream of the pcbC gene encoding 2,3- dihydroxybiphenyl 1,2-dioxygenase, and approximately 4-kb upstream of the pcbE gene encoding HPD hydratase. The pcbK gene was able to encode a polypeptide with a molecular weight of 31,732 containing 284 amino acid residues. The deduced amino acid sequence of the HOPDA hydrolase of Pseudomonas sp. P20 exhibited high identity (62%) with those of the HOPDA hydrolases of P. putida KF715, P. pseudoalcaligenes KF707, and Burkholderia cepacia LB400, and also significant homology with those of other hydrolytic enzymes including esterase, transferase, and peptidase.

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Effect of the Various Heavy Metals on the Growth and Phosphorus (P) Removal Capacity of the Phosphorus Accumulating Microorganism (Pseudomonas sp.) (다양한 중금속이 인 축적 미생물 (Pseudomonas sp.)의 생장과 인 제거에 대한 효과)

  • Kim, Hee-Jung;Yoo, Ri-Bi;Han, Seok-Soon;Woo, Sun-Hee;Lee, Moon-Soon;Baek, Ki-Tae;Chung, Keun-Yook
    • Korean Journal of Environmental Agriculture
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    • v.29 no.2
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    • pp.189-196
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    • 2010
  • The removal of phosphorus (P) in the wastewater is essential for the prevention of eutrophication in the river and stream. This study was initiated to evaluate the effect of the various heavy metals on the growth and P removal capacity of Pseudomonas sp., which was well known as phosphorus accumulating microorganism(PAO's) in the EBPR(Enhanced Biological Phosphorus Removal) process. The five heavy metals used in the study were Cu, As, Zn, Ni, and Cd. The growth rate of Pseudomonas sp. was the greatest at $25^{\circ}C$, but the removal efficiency of P was the highest at $30^{\circ}C$. The $IC_{50}$ (median Inhibition Concentration) values of Pseudomonas sp. for the Cu, As, Zn, Ni, and Cd were 2.35, 11.04, 1.80, 4.92, and 0.24 mg/L, respectively. Therefore, it appears that the sensitivity of the heavy metals to Pseudomonas sp. was in the following order: Cd> Zn> Cu> Ni> AS. Also, the P removal efficiencies by Pseudomonas sp. were correspondingly decreased as the concentrations of heavy metals were increased.

Cloning of phnQ Gene Encoding Extradiol Dioxygenase from Pseudomonas sp. DJ77 and Its Expression in Escherichia coli (Pseudomonas sp. DJ77 균주에서 Extradiol Dioxygenase를 암호화하는 phnQ 유전자의 클로닝과 대장균에서의 발현)

  • 신희정;박용춘;민경희;김치경;임재윤;김영창
    • Korean Journal of Microbiology
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    • v.33 no.1
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    • pp.22-26
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    • 1997
  • We cloned the 5~kb Xlwl fragment containing gene responsible for degrad"tion of phenanthrene using pBLUES~ CRIPT SK( +) vector and E. coli XLI-Blue strain from the genomic library of Pseudomonas sp. 0177 and this recombinant plasmid was named pUPX5. The strain containing pUPX5 could produce a yellow meta-cleavage product using 2.3-dihydroxybiphenyl as a substrate. This strain have a higher activity toward 2,3-dihydroxybiphenyl than catechol. We sub cloned and localized the gene encoding 2.3-dihydroxybiphenyl-1.2-dioxygenase. which is designated as phn$\Omega$.

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Isolation and Characterization of Pseudomonas sp. P2 Degrading Polychlorinated Biphenyls (PCBs)

  • Kim, Jung Ho;Sang Ki Choi;Moon Ki Park;Young Ho Kim;Seung Kyo Suh;Cheol Joo Woo;Heui Dong park
    • Journal of Microbiology and Biotechnology
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    • v.6 no.3
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    • pp.167-172
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    • 1996
  • The bacterial strain P2 degrading polychlorinated biphenyls (PCBs) was isolated from the soil around the Shinchun stream in Taegu after enrichment culture in a media containing biphenyl as the sole carbon source. The isolate was identified as a strain of Pseudomonas sp. based on its morphological and physiological characteristics. The optimal conditions of initial pH of media and temperature for growth were 7.0 and $30^{\circ}C$, respectively. Degradation of biphenyl and PCBs was confirmed by GC during the culture of Pseudomonas sp. P2 in a media containing them at a concentration of 500 mg/I. It was observed that Pseudomonas sp. P2 could degrade 97.0$%$ of biphenyl and 60.0$%$ of PCBs after 160 h culture.

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Characterization of Oil Degrading Bacterium Pseudomonas sp. BCK-1 Isolated from the Coastal Water of Yosu, Korea (여수주변해역에서 분리한 유류분해세균 Pseudomonas sp. BCK-1의 특성)

  • KOO Hoen-Seo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.2
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    • pp.145-150
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    • 2001
  • A marine bacterium having a high oil-degrading activity was isolated from the coastal water of Yosu, Korea, identified as Pseudomonas sp. and named Pseudomonas sp. BCK-1. The optimal temperature, pH and NaCl concentration for cell growth was $30^{\circ}C$, 7.0 and $3\%$ (w/v), respectively. After cultivation at $30^{\circ}C$, 180 rpm in 250 mL erlenmeyer flask for 72 and 168 hours, $2\%$ (w/v) arabian light crude oil (ACO) and bunker C oil (BCO) which are considered to be hardly biodegradable compounds were degraded $92\%$ (w/w) and $72\%$ (w/w), respectively.

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Characterization of Bacteria Isolated from Rotted Onions (Allium cepa) (양파 부패병변에서 분리한 세균의 특성)

  • Lee Chan-Jung;Lim Si-Kyu;Kim Byung-Chun;Park Wan
    • Microbiology and Biotechnology Letters
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    • v.33 no.4
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    • pp.248-254
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    • 2005
  • One hundred thirty nine bacteria were isolated from rotten onions collected from main producing districts, Chang-Nyung, Eui-Ryung, and Ham-Yang in Korea. The $18\%$ (25 strains) of bacterial isolates have carboxymethylcellulase (CMCase) activity and the $53\%$ (74 strains) have polygalacturonase (PGase) activity. Thirty one among randomly selected 45 strains of PGase producing bacteria have pathogenicity to onions. The isolates were classified into Pseudomonas sp. (18 strains), Bacillus sp. (11 strains), Yers-inia sp. (7 strains), and others (9 strains) on the basis of FAMEs patterns. Eighteen strains of Pseudomonas sp. were mainly divided into three cluster in the dendrogram and only the two clusters of them showed pathogenicity to onions. CMCase and PGase activities of Pseudomonas sp. weaker than those of Bacillus sp.. However, the pathogenicity of pseudomonas sp. to soften onions was stronger than that of Bacillus sp. Inoculation of $10^{2}$ cfu of Pseudomonas sp. gives rise to softening of onions. Pseudomonas sp. was identified as Pseudomonas gladioli by biochemical and physiological characteristics. P. gladioli is the first reported bacterium as a pathogen of onion in Korea. In low temperature, P. gladioli showed better growth and higher PGase activity than those of Bacillus sp. identified as Bacillus subtilis. And pH 9.0 is optimal pH for PGase activity of B. subtilis while that of P. gladioli is pH $5.0\∼6.0$ which is the acidity of onions. Taken together, P. gladioli may be a main pathogene of onion rot during the cold storage condition.

Organic Solvent-tolerant Lipase from Pseudomonas sp. BCNU 154 (Pseudomonas sp. BCNU 154 유래의 유기용매 내성 리파아제)

  • Choi, Hye Jung;Hwang, Min Jung;Seo, Jeoung-Yoon;Joo, Woo Hong
    • Journal of Life Science
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    • v.23 no.10
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    • pp.1246-1251
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    • 2013
  • An organic solvent-tolerant lipase of Pseudomonas sp. BCNU 154 that was isolated from wastewater in the industrial complex region had optimal activity at $37^{\circ}C$ and pH 8. This crude extracellular lipase from BCNU 154 exhibited maximum stability in toluene, retaining about 6.01 U/ml (117.53%) activity for 2 h. $Ca^{2+}$, $Mg^{2+}$, $NH_4{^+}$, and $Na^+$ ions and triton X-100 activated the enzymes, whereas $Ba^{2+}$, $Hg^{2+}$, and $Zn^{2+}$ ions inhibited their activity. Pseudomonas sp. BCNU 154 lipase revealed stable activity comparable to that of the commercial immobilized Novozym 435. Thus, this organic solvent-tolerant lipase could have potential as a whole cell biocatalyst in industrial chemical processes without the use of immobilization.

Characterization of Organic Solvent Stable Lipase from Pseudomonas sp. BCNU 106 (Pseudomonas sp. BCNU 106이 생산하는 유기용매 내성 리파아제의 특성)

  • Choi, Hye Jung;Hwang, Min Jung;Kim, Dong Wan;Joo, Woo Hong
    • Journal of Life Science
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    • v.26 no.5
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    • pp.603-607
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    • 2016
  • A crude extracellular lipase from solvent-tolerant bacterium Pseudomonas sp. BCNU 106 was highly stable in the broad pH range of 4-10 and at temperature of 37℃. Crude lipase of BCNU 106 exhibited enhanced stability in 25% organic solvents such as xylene (121.85%), hexane (120.35%), octane (120.41 %), toluene (118.14%), chloroform (103.66%) and dodecane (102.94%) and showed excellent stability comparable with the commercial immobilized enzyme. In addition, the stability of BCNU 106 lipase retained above 110% of its enzyme activity in the presence of Cu2+, Hg2+, Zn2+ and Mn2+, whereas Fe2+ strongly inhibited its stability. The detergents including tween 80, triton X-100 and SDS were positive signals for lipase stability. Because of its stability in multiple organic solvents, cations and surfactants, the Pseudomonas sp. BCNU 106 lipase could be considered as a potential biocatalyst in the industrial chemical processes without using immobilization.

Effect of Cosubstrate on tile Production of Poly(3-Hydroxybutyric-Co-3-Hydroxyvaleric) Acid from Glucose by Pseudomonas sp, HJ (Pseudomonas sp. HJ에 의한 포도당으로부터 Poly(3-Hydroxybutyric-Co-3-Hydroxyvaleric) Acid의 생합성에 대한 보조기질의 영향)

  • 손홍주;고명선이상준
    • KSBB Journal
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    • v.11 no.5
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    • pp.586-592
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    • 1996
  • Poly(3-hydroxybutyric-co-3-hydroxyvaleric) acid(PHB/HV) copolymer synthesis by Pseudomonas sp. HJ from glucose and cosubstrate was investigated. Taxonomic analysis suggested that Pseudomonas sp. HJ was best marched to Pseudomonas picketti having 78.8% similarity. Pseudomonas sp. HJ produced PHB/HV copolymer containing 60.8 mol% HV and 44.9 mol% HV when supplied with hexadecane and propionic acid as a cosubstrate, respectively. The HV composition in PHB/HV copolymer was controlled by varying the concentration of hexadecane and propionic acid. Propionic acid added after 24 hours of incubation was incorporated as the HV monomer in the PHB/HV copolymer up to 49.6 mol%.

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Antifungal Activity of Pseudomonas sp. DGUM 5051 Against Apple Bitter-rot Causing Fungus, Glomerella cingulata (사과탄저병균(Glomerella cingulata)에 대한 Pseudomonas sp. DGUM 5051의 항진균 활성)

  • Kim, Jung-Mi;Lee, Min-Woong;Han, Yeong-Hwan
    • The Korean Journal of Mycology
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    • v.26 no.4 s.87
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    • pp.458-465
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    • 1998
  • The strain DGUM 5051, an antagonistic bacterium against apple-bitter rot causing Glomerella cingulata, was isolated from soil in Kyongju. Based on the morphological and physiological characteristics, the bacterium was identified as Pseudomonas sp. and named as Pseudomonas sp. DGUM 5051. The optimal pH and temperature for cell growth were pH 6.0 and $30^{\circ}C$, whereas those for antifungal activity were pH 7.0 and $24^{\circ}C$, respectively. Among the complex media tested, brucella medium, brain heart infusion medium and Luria-Bertani medium were good for both cell growth and antifungal activity. The high antifungal activity was found in the mineral salts medium, in which sucrose, $KNO_3$ and $K_2HPO_4$ were used as sources of carbon, nitrogen and phosphorus, respectively.

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