• 미생물학회지
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• 제33권1호
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• pp.22-26
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• 1997

Pseudomonas sp. DJ77의 게놈 library로부터 phenanthrene 분해에 관련된 유전자를 포함하는 약 5-kb의 XhoI 절편을 pBLUESCRIPT SK(+)로 클로닝하였으며, 이 재조합 plasmid를 pUPX5라 명명하였다. 이 재조합 균주에 catechol과 2,3-dihydroxybiphenyl 용액을 분무하면 노란색의 meta-cleavage 화합물이 생성됨을 관찰 할 수 있었다. 그리고 효소 활성을 측정한 결과 catechol에서보다 2,3-dihydroxybiphenyl에 더 큰 활성을 나타냈다. 이 부분에 존재하는 2,3-dihydroxybiphenyl 1,2-dioxygenase 유전자의 위치를 결정하고, 이 유전자를 phnQ라 명명하였다.

• 한국환경과학회지
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• 제11권3호
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• pp.177-182
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• 2002

A biosurfactant-producing microorganism was isolated from activated sludge by enrichment culture when grown on a minimal salt medium containing n-hexadecane as a sole carbon source. This microorganism was identified as Pseudomonas sp. and it was named Pseudomonas sp. EL-G527. It's optimal culture condition is 2% n-hexadecane, 0.2% NH$_4$NO$_3$, 0.3% KH$_2$PO$_4$, 0.3% $K_2$HPO$_4$, 0.02% MgSO$_4$ㆍ7$H_2O$, 0.0025% CaCk$_2$ㆍ6$H_2O$, 0.0015% FeSO$_4$ㆍ7$H_2O$ in 1$\ell$ distilled water and initial pH 7.0. Cultivation was initiated with a 2% inoculum obtained from starter cultures grown in 30 $m\ell$ of the same medium in 250 $m\ell$ flask. They were cultivated at 3$0^{\circ}C$ in reciprocal shaking incubator and the highest biosurfactant production was observed after 4 days.

• Journal of Microbiology and Biotechnology
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• 제8권6호
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• pp.692-695
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• 1998

Pseudomonas sp. DJ-12 was able to degrade 4-chlorobenzoate by hydrolytic dechlorination to produce 4-hydroxybenzoate and chloride ion. The fcbABC genes responsible for the hydrolytic dechlorination were cloned from the chromosomal DNA of the organism. The genes were found to be organized in the order fcbB-fcbA-fcbC, but there was an intergenic space between the fcbA and fcbC genes.

• Journal of Microbiology
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• 제37권3호
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• pp.123-127
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• 1999

A Pseudomonas sp. strain DJ-12 isolated by 4-cholrobiphenyl enrichment culture technique is capable of utilizing 4-hydroxybenzoic acid as a sole source of carbon and energy. The bacterium catabolized 4-hydroxybenzoic acid through the intermediate formation of protocatechuic acid, which was further metabolized. The cell free extracts of pseudomonas sp. DJ-12, grown on 4-hydroxybenzoic acid showed higher activities of 4-hydroxyenzoate 3-hydroxylase and protocatechuate 4,5-dioxygenase, but the activity of catechnol 2,3-dioxygenase was lower. The results suggest that 4-hydroxybenzoic acid is catabolized via protocatechuic acid rather than catechol or gentisic acid in this bacterium and that the protocatechuic acid formed was metabolized through a metacleavage pathway by protocatechuate 4,5-dioxygenase.

• 한국미생물·생명공학회지
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• 제19권6호
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• pp.631-636
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• 1991

방향족화합물을 생분해하는 미생물을 분리하여 생물학적 처리에 응용하기 위해 폐수 및 토양에서 150종의 균을 분리하였다. 그 중에서 COD 제거율과 방향족화합물의 이용능이 가장 우수한 HC107균을 선발하여 Pseudomonas sp.로 동정하였다. 활성슬러지 장치에서 Pseudomonas sp. HC107 배양액을 2ml/day씩 처리하면서 화학, 제약 및 도료공장의 폐수를 혼합하여 연속처리한 결과 처리수의 COD, BOD 및 phenol 제거율이 평균 92.5%, 95.53 및 93%.5로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.406-411
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• 2001

In a bacterial denitrification test with Pseudomonas sp. and anaerobic consortium, more nitrates and less substrate were consumed but less metabolic nitrite was produced under an anaerobic $H_2$ condition rather than under $N_2$ condition. In a bioelectrochemical denitrification test with the same organisms, the electrochemically reduced neutral red was confirmed to be a substitute electron donor and a reducing power like $H_2$. The biocatalytic activity of membrane-free bacterial extract, membrane fraction, and intact cell for bioelectrochemical denitrification was measured using cyclic voltammetry. When neutral red was used as an electron mediator, the electron transfer from electrode to electron acceptor (nitrate) via neutral red was not observed in the cyclic voltammogram with the membrane-free bacterial extract, but it was confirmed to gradually increase in proportion to the concentration of nitrate in that of the membrane fraction and the intact cell of Pseudomonas sp.

• Journal of Microbiology and Biotechnology
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• 제8권1호
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• pp.96-100
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• 1998

Pseudomonas sp. S-47 degraded 4-chlorobenzoate (4CBA) to 4-chlorocatechol (4CC) that was subsequently ring-cleaved to form 5-chloro-2-hydroxymuconic semialdehyde. These intermediate compounds were identified by GC-mass spectrometry and UV-visible spectrophotometry. 5-chloro-2-hydroxymuconic acid converted from 5-chloro-2- hydroxymuconic semialdehyde (5C-2HMS) was dechlorinated to produce 2-hydroxypenta-2,4-dienoic acid (2HP-2,4DA) by the strain. These results indicate that Pseudomonas sp. S-47 degrades 4CBA to 2HP-2,4DA via a novel pathway including the meta-cleavage of 4CC and dechlorination of 5C-2HMS.

• 한국미생물·생명공학회지
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• 제21권2호
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• pp.150-156
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• 1993

The 2,3-dihydroxybiphenyl(2,3-DHBP) dioxygenase, the product of pcbC gene, was purified from the biphenyl and 4-chlorobiphenyl degrading Pseudomonas sp. DJ-12 by the methods of acetone precipitation, DEAE-Sephadex A-50 ion exchange chromatography, and Sephadex G-150 gel filtration chromatography. The enzyme was estimated to be about 260 kilodaltons in molecular weight and to be consisted of eight subunits. The Km value of the enzyme was 61 nM to 2,3-DHBP and the highest activity of the enzyme was observed at pH 8 and 30C.

• 미생물학회지
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• 제24권4호
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• pp.389-393
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• 1986

염소계 방향족 탄화수로를 분해하는 다수의 세균을 토양과 하천 포품으로부터 분리하여 동정하였다. 이들중 2-메틸-4-클로로페녹시 아세트산을 강력하게 분해하고, 또한 플라스미드를 갖고 있는 7군주의 Pseudomonas sp.를 선별하였다. 2-메틸-4-클로로페녹시 아세트산의 분해 유전자가 플라스미드에 기인함을 전기영동과 큐어링 실험에 의해서 확이하였다. 항생물질에 대한 내성과 다른 제초제에 대한 분해능도 아울러 조사하였다.

• 미생물학회지
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• 제41권1호
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• pp.13-17
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• 2005

본 연구에서는 이 전 연구에서 단일 탄소원과 질소원 및 에너지원으로 aniline을 이용하는 Delftia sp. JK-2에서 분리, 정제된 바 있는 C2,3O의 N-말단 아미노산과 DNA 서열을 분석하였다. Aniline에서 배양한 Delftia sp. JK-2에서 분리된 약 35kDa의 C2,3O의 N-말단 아미노산 서열을 분석 한 결과 $^1MGVMRIGHASLKVMDMDAAVRHYENV^{26}$로 Pseudomonas sp. AW-2와 Comamonas sp. JS765의 C2,3O와 일치하는 것으로 나타났다. 위에서 확인된 아미노산 서열을 바탕으로 제작된 primer와 JK-2의 total genomic DNA를 기질로 사용하여 PCR을 수행한 결과 약 950 bp의 유전자 증폭산물을 획득하였다. 이 증폭산물 중 정확히 확인된 890 bp의 염기서열을 분석한 결과 Delftia JK-2의 C2,3O유전자 염기서열은 Pseudomonas su. AW-2의 C2,3O와 일치하였으며 Comamonas sp. Js765의 C2,3O와 $97\%$의 높은 상동성을 나타내었다.