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http://dx.doi.org/10.5322/JES.2002.11.3.177

Optimal Culture Conditions for Production of Environment-Friendly Biosurfactant by Pseudomonas sp. EL-G527  

차미선 (부산대학교 미생물학과)
임은경 (부산대학교 미생물학과)
이근희 (부산대학교 미생물학과)
조순자 (부산대학교 미생물학과)
손홍주 (밀양대학교 생물공학과)
이상준 (부산대학교 미생물학과)
Publication Information
Journal of Environmental Science International / v.11, no.3, 2002 , pp. 177-182 More about this Journal
Abstract
A biosurfactant-producing microorganism was isolated from activated sludge by enrichment culture when grown on a minimal salt medium containing n-hexadecane as a sole carbon source. This microorganism was identified as Pseudomonas sp. and it was named Pseudomonas sp. EL-G527. It's optimal culture condition is 2% n-hexadecane, 0.2% NH$_4$NO$_3$, 0.3% KH$_2$PO$_4$, 0.3% $K_2$HPO$_4$, 0.02% MgSO$_4$ㆍ7$H_2O$, 0.0025% CaCk$_2$ㆍ6$H_2O$, 0.0015% FeSO$_4$ㆍ7$H_2O$ in 1$\ell$ distilled water and initial pH 7.0. Cultivation was initiated with a 2% inoculum obtained from starter cultures grown in 30 $m\ell$ of the same medium in 250 $m\ell$ flask. They were cultivated at 3$0^{\circ}C$ in reciprocal shaking incubator and the highest biosurfactant production was observed after 4 days.
Keywords
Pseudomonas sp. EL-G527; biosurfactant; activated sludge; n-hexadecane;
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