• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.5
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• pp.380-384
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• 2004

The toxic impact of parathion on bagrid catfish (Pseudobagrus fulvidraco) juvenile was examined 96 hr $LC_{50}$ value of parathion estimated as 1 90 mg/L. Chronically, bagrid catfish were exposed for 30 days to concentrations of parathion, i.e. 63, 95, 190 and $380\;{\mu}g/L$, representing 3.3, 5.0, 10.0 and $20.0{\%}$ of the 96 hr $LC_{50}$. Survival rate was significantly affected by the concentration over $63\;{\mu}g/L$ after 10 days. Growth rate, specific growth rate (SGR) and feed efficiency were reduced in time as concentration dependent manner. Growth rates of the exposed groups were significantly reduced as $28.06-60.63{\%}$ to the control fish, SGR was decresed as $1.50-4.72{\%}$ and feed efficiency was declined as $6.10-11.90{\%}$. It can be concluded that if p. lulvidraco is chronically exposed to parathion over $63\;{\mu}g/L$ concentration, the physiological damages might affect growth factors of the fish.

• Fisheries and Aquatic Sciences
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• v.7 no.3
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• pp.109-114
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• 2004

To evaluate the impact of parathion on aquatic organisms, a freshwater bagrid catfish (Pseudobagrus fulvidraco) was exposed to sublethal concentrations $(63,\;95,\;190\;and\;380\;{\mu}g/L)$ of parathion, organophosphorus pesticide for 30 days. Glucose level in the serum of the bagrid catfish was significantly increased than that of control groups in the $190\;{\mu}g/L$ concentration at 30 days and in the $380\;{\mu}g/L$ concentration after 10 days. Bilirubin level was significantly increased in the $190\;{\mu}g/L$ concentration at the end of the experiment. After 10 days, a significant differences of lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activity increased in the ${\geq}190\;{\mu}g/L$ and $380\;{\mu}g/L$ groups. Though cholesterol concentration was stable, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in serum were significantly reduced in the $190\;{\mu}g/L$ at 10 days and in the $380\;{\mu}g/L$ concentration at the end of the experiment. In conclusion, if P. fulvidraco was chronically exposed to ${\geq}190\;{\mu}g/L$ concentration of parathion, the hematological changes may be induced due to the stress response.

• Journal of Aquaculture
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• v.21 no.4
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• pp.285-293
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• 2008

Vitellogenin (Vg) is the precursor of vitellin (Vn), the major yolk protein of teleost fishes. In this study, Vg and Vn proteins of the Korean bullhead Pseudobagrus fulvidraco were isolated using gel-filtration chromatography (Sephadex-G 200 column) and anion-exchange chromatography (Mono Q HR 5/5 column), respectively. Purified Vn with an estimated molecular mass of 360 kDa by gel filtration chromatography was obtained from ovarian egg, and it was composited to one major subunit with an estimated molecular mass of 107 kDa by SDS-PAGE. In the result of western blotting, one major band was detected using antiserum against Vn (anti-Vn). These results suggested that Vn was composed of three subunits having the same molecular weight in Pseudobagrus fulvidraco. Vg was induced by estradiol-$17{\beta}$ ($E_2$) and purified from $E_2$ treated male serum. The molecular weight of whole Vg was estimated to be 450 kDa by gel filtration chromatography, and it is composed of three subunits with estimated molecular masses of 110 kDa, 125 kDa and 147 kDa as determined by SDS-PAGE. In the Ouchterlony's immunodiffusion test using anti-Vn and antiserum against female and male serum, purified Vg was detected in matured female and Ez treated male serum but not in untreated male. These results can be used in detecting estrogenic contamination of the aquatic environment.

• Environmental Engineering Research
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• v.9 no.4
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• pp.154-159
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• 2004

• Korean Journal of Fisheries and Aquatic Sciences
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• v.38 no.5
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• pp.304-308
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• 2005

The effects of diethylhexyl phthalate (DEHP) on various oxidative stress responses in liver, kidney and gill tissues of freshwater bagrid catfish Pseudobagrus fulvidraco were investigated under laboratory conditions. Bagrid catfish were intraperitoneally injected with sunflower seed oil containing nominal concentrations of 0, 300 or 900mg DEHP per kilogram of body weight for 3 days and the effects after last injection were assessed in liver, kidney and gill tissues of the exposed organisms. The oxidative stress responses of fish were evaluated by analyzing the level of glutathione (GSH), as well as the activities of antioxidant enzymes such as glutathione S-transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GR). After exposure to the DEHP, there were significant decrease in GR, GPx activity and GSH content in liver of fish exposed to 900 mg DEHP per kilogram of body weight compared to the control group. Compared with the control group, significant decreases in renal GPx and GR activity were observed in the DEHP treatment groups (900 mg $kg^{-1}$ bw). However, no significant difference was observed in any oxidative stress responses in gills between the DEHP-treated and the untreated group of fish. The findings of the present investigation show that DEHP induce oxidative stress and the liver was the most affected organ followed by the kidney and gills. Furthermore, the changes of GPx and GR activities may be important indicators of oxidative stress responses but additional study is required to confirm the oxidative stress of DEHP.

• Korean Journal of Ichthyology
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• v.8 no.2
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• pp.10-15
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• 1996

The chromosome numbers of bagrid catfish, Pseudobagrus fulvidraco was 52, nine pairs (No. 1 to 9) were metacentrics with the range of relative length 2.89~6.22 and arm ratio 1.09~1.58 ; thirteen pairs (No. 10 to 22) were submetacentrics with the range of relative length 2.88~5.88 and arm ratio 1.80~3.65 ; and all other pairs (No. 23 to 26) were acrocentrics with the range of relative length 2.63~3.30 and arm ratio 9.01~10.67, and fundamental number was 104. Heteromorphic sex chromosomes were not found. There was not exist significant difference in resultant erythrocyte measurements and parameters between female and male (p<0.05). The mean sizes of cell and nucleus, were $11.03{\times}9.67{\mu}m$ and $4.18{\times}3.66{\mu}m$ respectively. The number of erythrocytes of both females and males were $6{\sim}7{\times}10^5/ml$. Gill tissues from diploid individuals had cells with one or two nucleoli.

• Journal of Fisheries and Marine Sciences Education
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• v.26 no.2
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• pp.245-256
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• 2014

잉어, Cyprinus carpio와 동자개, Pseudobagrus fulvidraco의 상악 하악 수염을 조직학적으로 조사하였다. 동자개의 수염은 연골성 증축(axial rod of cartilage), 신경섬유다발(bundle of nerve fiber), 표피(epidermis), 평활근 층(smooth muscle layer) 및 미뢰(taste bud)로 구성되었으며, 잉어의 수염은 표피, 신경섬유다발, 혈관(blood vessel) 및 미뢰로 구성되었다. 수염 길이에서 잉어는 상악 바깥쪽 수염(second maxillary barbel)이 상악 안쪽 수염(first maxillary barbel) 보다 길게 나타났으며, 동자개는 하악 안쪽(inner mandibular barbel), 상악 위쪽(upper maxillary barbel), 하악 바깥쪽(outer mandibular barbel), 상악 아래쪽(lower maxillary barbel) 순으로 길게 나타났다(P<0.05). 미뢰의 수를 고려하였을 때, 동자개와 잉어간의 미각에 대한 유의적 차이가 없었다(P>0.05). 아울러, 두 어종의 모든 수염에서 수염 상부의 미뢰 수가 하부의 미뢰 수 보다 높게 나타났다(P<0.05). 본 연구 결과, 동자개의 수염은 딱딱하며 굴절성인 수염(flexible and stiff type)이었으며 잉어의 수염은 연하고 유연한 수염(tender and yielding type)으로 파악되었다.

• Applied Microscopy
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• v.46 no.3
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• pp.150-154
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• 2016

The ultrastructure of fertilized egg envelope from Pseudobagrus fulvidraco belongs to Bagridae was investigated using light and electron microscopes. The fertilized egg was compressed spherical, light-yellowish, demersal, and adhesive. The size of fertilized egg is about $1.85{\pm}0.13mm$, perivitelline space is not well developed, and there were no appendicular structures on the outer surface of egg envelope and oil droplets in vitelline membrane under light microscope. The micropyle was located in the animal pole of fertilized egg. Adhesive reticular fiber was covered fertilized egg envelope. The thickness of egg envelope was about $3.7{\sim}4.2{\mu}m$, and the egg envelope consisted of two layers: an outer, electron-dense adhesive fibers layer and an simple inner layer with pore. Therefore, the ultrastructure of cross section of the fertilized egg envelope showed species specificity, but studies on the other species belongs to Bagridae were need to get correct information about common traits in family.

• Korean Journal of Ichthyology
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• v.13 no.2
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• pp.93-99
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• 2001

Skin mucous cells in the three regions of the body in Pseudobagrus fulvidaraco and Leiocassius nitidus were investigated using three histochemical methods (PAS, AB-PAS and HID). In the two species, components of mucous cell were not distinguished in each region, but presented a differences in position and type, size, and count in all regions. Mucous cells of P. fulvidraco were located in two layers, a superficial epithelial cell and the space within alarm substance cells (ASCs). Mucous cells of L. nitidus were situated only in the superficial epithelial cell layer. The size of mucous cells in the ASC layer were larger than those of the superficial epithelial cells for all measured values: diameter, length, width, and area. In the superficial epithelial cell layer, the average number of mucous cells within the three regions did not differ significantly between species by unpaired T-test, but the average number of those within two cell layers of P. fulvidraco had a similarity at each region by paired T-test. The dismilarity in dorsal and ventral regions in average number of ASCs between P. fulvidraco and L. niditus was confirmed by unpaired T-test.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.5
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• pp.823-833
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• 1997

To clarify the annual reproductive cycle of the banded catfish, Pseudobagrus fulvidraco (Richardson), the seasonal changes in histological aspect of gonad and liver were examined. The adult fish was raptured from the upper stream of Young-San river, Chunnam in each month from May 1992 to June 1993. Based on the annual changes in GSI (gonadosomatic index), HSI (hepatosomatic index), CF (condition factor) and histological aspects of the gonads, the annual reproductive cycle were classified into 5 periods as follows: 1) Growing phase (from April to early May): The value of GSI increased and the size of oocytes in perinucleolus stage in oocytes increased gradually. Spermatogonia were developed actively from the epithelial tissues of seminiferous tubules. 2) Maturing phase (from Hay to early June): GSI levels increased rapidly in both sex. Oocytes at various developmental stages were observed. Appearance of active spermatogenesis were observed. 3) Mature and spawning phase (from June to August): High values of GSI remained static and oocytes accumulated significant quantitis of yolk globules. 4) Degenerating phase (from September to November): GSI levels decreased and ovaries were filled mostly with oocytes at the perinucleolus stage. Hepatic cells accumulated significant amounts of lipid droplets. 5) Resting period (from December to March) : Low values of GSI were kept and the size of oocytes at the perinucleolus stage did not increase. Spermatogenesis was not observed.