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http://dx.doi.org/10.5657/kfas.2005.38.5.304

Changes of Antioxidant Enzyme Activity in Bagrid Catfish, Pseudobagrus fulvidraco Exposed to Diethylhexyl Phthalate  

KEUM Yoo-Hwa (Department of Aquatic Life Medicine, Pukyong National University)
JEE Jung-Hoon (Institute of Fisheries Sciences, Pukyong National University)
KOO Ja-Geun (Incheon Fisheries Hatchery Research Institute)
KANG Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.38, no.5, 2005 , pp. 304-308 More about this Journal
Abstract
The effects of diethylhexyl phthalate (DEHP) on various oxidative stress responses in liver, kidney and gill tissues of freshwater bagrid catfish Pseudobagrus fulvidraco were investigated under laboratory conditions. Bagrid catfish were intraperitoneally injected with sunflower seed oil containing nominal concentrations of 0, 300 or 900mg DEHP per kilogram of body weight for 3 days and the effects after last injection were assessed in liver, kidney and gill tissues of the exposed organisms. The oxidative stress responses of fish were evaluated by analyzing the level of glutathione (GSH), as well as the activities of antioxidant enzymes such as glutathione S-transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GR). After exposure to the DEHP, there were significant decrease in GR, GPx activity and GSH content in liver of fish exposed to 900 mg DEHP per kilogram of body weight compared to the control group. Compared with the control group, significant decreases in renal GPx and GR activity were observed in the DEHP treatment groups (900 mg $kg^{-1}$ bw). However, no significant difference was observed in any oxidative stress responses in gills between the DEHP-treated and the untreated group of fish. The findings of the present investigation show that DEHP induce oxidative stress and the liver was the most affected organ followed by the kidney and gills. Furthermore, the changes of GPx and GR activities may be important indicators of oxidative stress responses but additional study is required to confirm the oxidative stress of DEHP.
Keywords
Antioxidant enzyme; Di-ethylhexyl phthalate (DEHP); Pseudobagrus fulvidraco; Oxidative stress;
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  • Reference
1 USEPA (U.S. Environmental Protection Agency). 1999. Integrated Risk Information System (IRIS) on Di (2-ethylhexyl) phthalate, National Center for Environmental Assess- ment, Office of Research and Development, Wa- shington, DC
2 Winston, G.W. and R.T. Di Giulio. 1991. Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat. Toxicol., 19, 137-161   DOI   ScienceOn
3 Worthington, D.J. and M.A. Rosemeyer. 1974. Human glutathione reductase: Purification of the crystalline enzyme from erythrocytes. Eur. J. Biochem., 48, 167-177   DOI   ScienceOn
4 Zhang, J.F., X.R. Wang, H.Y. Guo, J.C. Wu and Y.Q. Xue. 2004. Effects of water-soluble fractions of diesel oil on the antioxidant defenses of the goldfish, Carassius auratus. Ecotoxicol. Environ. Saf., 58, 110-116   DOI   ScienceOn
5 Keppler, D., I. Leier and G. Jedlitschky. 1997. Transport of glutathione conjugates and glucuronides by the multidrug resistance proteins MRP1 and MRP2. Biol. Chem., 378, 787-791
6 Casajuana, N. and S. Lacorte. 2004. New methodology for the determination of phthalate esters, bisphenol A, bisphenol A diglycidyl ether, and nonylphenol in commercial whole milk samples. J. Agric. Food Chem., 52, 3702-3707   DOI   ScienceOn
7 Radi, A.A.R., D.Q. Hai, B. Matkovics and T. Gabrielak. 1985. Comparative antioxidant enzyme study in fresh water fish with different types of feeding behavior. Comp. Biochem. Physiol., 81, 395-399   DOI   ScienceOn
8 Mannervik, B. 1985. Glutathione peroxidase. Meth. Enzymol., 113, 490-495   DOI
9 Watanuki, H., Y. Gushiken and M. Sakai. 2003. In vitro modulation of common carp (Cyprinus carpio L.) phagocytic cell by di-n-butyl phthalate and di-2-ethylhexyl phthalate. Aquat. Toxicol., 63, 119-126   DOI   ScienceOn
10 Li, W.M., D.Q. Yin, Y. Zhou, S.Q. Hu and L.S. Wang. 2003. 3,4-Dichloroaniline-induced oxidative stress in liver of crucian carp (Carassius auratus). Ecotoxicol. Environ. Saf., 56, 251-255   DOI   ScienceOn
11 Mather-Mihaich, E. and R.T. Di Giulio. 1991. Oxidant, mixed-function oxidase and peroxisomal responses in channel catfish exposed to a bleached kraft mill effluent. Arch. Environ. Contam. Toxicol., 20, 391-397   DOI   ScienceOn
12 Rudneva-Titova, I.I. and N.V. Zherko. 1994. Effects of polychlorinated biphenyls on the activity of antioxidant enzymes and lipid peroxidation in muscle and liver of two Black Sea fish species. Biochemistry-Moscow, 59, 25-31
13 O'Brien, M.L., M.L. Cunningham, B.T. Spear and H.P. Glauert. 2001. Effects of peroxisome proliferators on glutathione and glutathione-related enzymes in rats and hamsters. Toxicol. Appl. Pharmacol., 171, 27-37   DOI   ScienceOn
14 Otto, D.M.E. and T.W. Moon. 1995. 3,3',4,4'-tetrachlorobiphenyl effects on antioxidant enzymes and glutathione status in different tissues of rainbow trout. Parmacol. Toxicol., 77, 281-287
15 Reddy, J.K. 1990. Carcinogenicity of peroxisomal proliferators: Evaluation and mechanisms. Biochem. Soc. Trans., 18, 92-94   DOI
16 Sayeed, I., S. Parvez, S. Pandey, B. Bin-Hafeez, R. Haque and S. Raisuddin. 2003. Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch. Ecotoxicol. Environ. Saf., 56, 295-301   DOI   ScienceOn
17 Seo, K.W., K.B. Kim, Y.J. Kim, J.Y. Choi, K.T. Lee and K.S. Cho. 2004. Comparison of oxidative stress and changes of xenobiotic metabolizing enzymes induced by phthalates in rats. Food Chem. Toxicol., 42, 107-114   DOI   ScienceOn
18 Stephensen, E., J. Sturve and L. Forlin. 2002. Effects of redox cycling Compounds on glutathione content and activity of glutathione-related enzymes in rainbow trout liver. Comp. Biochem. Physiol., 133, 435-442
19 Hamed, R.R., S.E. Elawa and N.M. Farid. 1999. Evaluation of detoxification ebzyme levels in Egyptian catfish, Clarias lazera, exposed to dimethoate. Bull. Environ. Contam. Toxicol., 63, 789-796   DOI   ScienceOn
20 Han, K.N., K.B. Nam and C.H. Jeong. 2001. Development of eggs, larvae and juvenile of the Korean bullhead, Pseudobagrus fitlvidraco (Richardson) reared in the laboratory. Korean. J. lchthyol., 13, 74-84
21 Kaplowitz, N., J.C., Femandez-Checa, R. Kannan, C. Garcia-Ruiz, M. Ookhtens and J.R. Yi. 1996. GSH transporters: molecular characterization and role in GSH homeostasis. Biol. Chem. Hoppe. Seyler, 377, 267-273
22 Hasspieler, B.M., J.V. Behar and R.T. Di Giulio. 1994. Glutathione dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurius nebulosus). Ecotoxicol. Environ. Saf., 28, 82-90   DOI   ScienceOn
23 James, N.H., A.R. Soames and R.A. Roberts. 1998. Suppression of hepatocyte apoptosis and induction of DNA synthesis by the rat and mouse hepatocarcinogen diethylhexylphlathate (DEHP) and the mouse hepatocarcinogen 1,4-dichlorobenzene (DCB). Arch. Toxicol., 72, 784-790   DOI   ScienceOn
24 Jobling, S., T. Reynolds, R. White, M.G. Parker and J.P. Sumpeter. 1995. A variety of environmentally persistent chemicals, including some phthalate plasticizers, are weakly estrogenic. Environ. Health Perspect., 103, 582-587   DOI   ScienceOn
25 Kappus, H. 1985. Lipid peroxidation; mechanisms, analysis, enzymology and biological relevance. In: Oxidative Stress. Sies, H., ed. Academic Press, London, pp. 273-310
26 Karle, V.A., B.L. Short, G.R. Martin, D.I. Bulas, P.R. Getson, N.L.C. Luban, A.N. O'Brien and R.J. Rubin. 1997. Extracorporeal membrane oxygenation exposes infants to the plasticizer, di (2-ethyhexyl)phthalate. Crit. Care. Med., 25, 696-703   DOI   ScienceOn
27 Bainy, A.C.D., E. Saito, P.S.M. Carvalho and B.V.C. Junquieira. 1996. Oxidative stress in gill, erythrocytes, liver andkidney of Nile tilapia (Oreochromis niloticus) from a polluted site. Aquat. Toxicol., 34, 151-162   DOI   ScienceOn
28 Beutler, E. 1984. Red cell metabolism. In: Manual of Biochemical Methods. 3rd ed., Grune and Starton, Inc. Orlando, USA, pp. 78-83
29 Baker, M.A., G.J. Cerniglia and A. Zaman. 1990. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal. Biochem., 190, 360-365   DOI   ScienceOn
30 Bell, J.G., C.B. Cowey, J.W. Adron and A.M. Shanks. 1985. Some effects of vitamin E and selenium deprivation on tissue enzyme levels and indices of tissue peroxidation in rainbow trout (Salmo gairdneri). Br. J. Nutr., 53, 149-157   DOI   ScienceOn
31 Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254   DOI   ScienceOn
32 Di Gilulio, R.T., P.C. Washburn and R.J. Wenning. 1989. Biochemical responses in aquatic animals; a review of determinants of oxidative stress. Environ. Toxicol. Chem., 8, 1103-1123   DOI
33 Di Giulio, R.T., C. Habig and E.P. Gallagher. 1993. Effects of black river harbour sediments on indices of biotransformation, oxidative stress, and DNA integrity in channel catfish. Aquat. Toxicol., 26, 1-22   DOI   ScienceOn
34 Fournier, D., J.M. Bride, M. Poirie, J.B. Berge and F.W. Plapp. 1992. Insect glutathione S-transferases: Biochemical characteristics of the major forms of houseflies susceptible and resistant to insecticides. J. Biol. Chem., 267, 1840-1845
35 Habig, W.H., M.J. Pabst and W.B. Jakoby. 1974. Glutathione S-Transferase. J. Bio. Chem., 249, 7130-7139